Direct evidence of the existence of specific LPS binding sites on vascular endothelial cells

(整期优先)网络出版时间:2004-02-12
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Objective:Toinvestigatethebindingcharacteristicsofendothelialcell(EC)withLPSfreefromtheparticipationofserumfactors.Methods:LaserconfocalmicroscopewasemployedintheobservationofthebindingofECwithFITC-LPS.TheKDandthebindingsitesofeachECwerecalculatedbyradioligandbindingassayofreceptors(RBA)using[3H]-LPS.Results:ThebindingofECwithLPSwassaturable,timeandconcentrationdependentanditcouldbecompetedwithoverdosedLPSofthesametype.Thefluorescencemainlydistributedincytoplasm,especiallynearthenucleus,whichcouldalsobestained.Conclusions:TheremightbesomespecificLPSbindingsitesexistingonEcsandLPScouldfunctionintracellularily.