IMMOBILIZATION OF GLUCOSE OXIDASE AND CELLULASE BY CHITOSAN— POLYACRYLIC ACID COMPLEX

(整期优先)网络出版时间:1990-02-12
/ 1
Thisstudyisconcernedwithchitosan-polyacrylicacidcomplexasacarriertoimmobilizeglucoseoxidase(GOD)andcellulase.TheoptimumemperatureoftheimmobilizedGOD(IG)wasdeterminedtobe60℃whichishigherthanthatofthenativeGODabout40℃.Theoptimumtemperatureoftheimmobilizedcellulase(IC)wasdeterminedtobeabout30℃higherthanthatofnativecellulase.BothoftheoptimumpHofIGandICshiftedonepHunittoacid.ImmobilizedenzymemaybeusedinmorewidepHrange.Theirstoragelifearemuchlongercomparedwiththeirnativestates.Bothofthemcanbereusedatleast12times.