简介:重建的向量pCANTAB5EE被插入包含了一个EcoRV识别序列进pCANTAB的34搁浅bpdouble的oligonucleotide获得5E。Whitespot症候群病毒(WSSV)染色体DNA被sonication碎裂主要在0的范围孤立碎片。8~2.0kb,当时,碎片与T4DNA聚合酶是钝结束的并且克隆5EE进pCANTAB的EcoRV地点。图书馆的主要recombinant克隆是随机的选择re-combinants的5.ColonyPCR显示出的3.0x10~inserts的尺寸是0.12~整个图书馆recombinant噬菌体感染了的1.77kb.AfterEscherichi--关口iHB2151房间,细胞外并且periplasmic摘录在PVDF膜上被扔执行点污点,usingpoly同种细胞的老鼠anti-VP24浆液,anti-WSV026浆液,anti-WSV063浆液,anti-WSV069浆液,anti-WSV112浆液,anti-WSV238浆液,anti-WSV303浆液和anti-VP26浆液结果证明显示图书馆能表示病毒的蛋白质。
简介:Whitespotsyndromevirus(WSSV)-resistantmolecularmarkerswerescreenedfromtheselectivelybrednewvariety‘HuanghaiNo.2’ofFenneropenaeuschinensisusingunlabeled-probehigh-resolutionmelting(HRM)technique.AftertheartificialinfectionwithWSSV,thefirst96deadshrimpsandthelast96survivingshrimpswerecollected,representingWSSV-susceptibleand-resistantpopulations,respectively.Thegenotypesatwell-developed39singlenucleotidepolymorphisms(SNPs)lociwereobtained.AsrevealedintheChi-squaretest,3SNPs,genotypeA/AofcontigC364-89AT,genotypeA/AofC2635-527CAandgenotypeC/TofcontigC12355-592CT,werepositivelycorrelatedwithdisease-resistancetraits.Other2SNPs,genotypeG/GofcontigC283-145AGandgenotypeC/CofcontigC12355-592CT,werenegativelycorrelated.Moreover,analysiswithBlastXprogramfordisease-resistantSNPsindicatedthat3contigs,Contig283,Contig364andContig12355,matchedtothefunctionalgenesofeffectorcaspaseofPenaeusmonodon,peptidetransporterfamily1-likeprotein,and40SribosomalproteinS2ofPercaflavescenswithhighsequencesimilarity.Theresultswillbehelpfultoprovidetheoreticalandtechnicalsupportsformolecularmarker-assistedselectivebreedingofF.chinensis.
简介:Whitespotsyndromevirus(WSSV)isoneofthemajorshrimppathogenscausinglargeeconomiclossestoshrimpfarming.Inanattempttoidentifytheenvelopeproteinsinvolvedinthevirusinfection,purifiedWSSVvirionsweremixedwiththreeantiseraagainstWSSVenvelopeproteins(VP39,VP124andVP187),individually.Andthentheywereinjectedintramuscularlyintocrayfish(Procambarusclarkii)toconductinvivoneutralizationassays.TheresultsshowedthatforgroupsinjectedwithvirionsonlyandgroupsinjectedwiththemixtureofvirionsandantiserumagainstVP124,thecrayfishmortalitieswere100%and60%onthe8thdaypostinfection,individually.ThevirusinfectioncouldbedelayedorneutralizedbyantibodyagainsttheenvelopeproteinVP124.QuantitativePCRwasusedtofurtherinvestigatetheinfluenceofthreeantiseradescribedaboveonthevirusinfection.TheresultsshowedthattheantiserumagainstVP124couldrestrainthepropagationofWSSVincrayfish.AlloftheresultssuggestedthattheviralenvelopeproteinVP124playedaroleinWSSVinfection.
简介:塑造耳朵的热前面(ESTF),由黄海温暖的水流(YSWC)和山东沿海的水流(SCC)的集中形成了,在冬季是在黄海(YS)的很重要的海洋的现象。在所有表明的situ大小和分析数据集,ESTF在1950s-1990s,和一个类似的变弱趋势期间一直在变弱,这是也在YS上在冬季季风发现。数字实验证明变弱冬季季风能沿着瓷器海岸和向南方的异常水流与向北方异常的水流在多十的timescale上在YS导致异常发行量在里面中央对季节的吝啬的循环YSgenerally相反。异常发行量引起YSWC和SCC的减速,并且因此削弱ESTF。因为ESTF在地区性的海洋动力学和由空至海的相互作用起重要作用,它变弱在冬季在YS为地区性的气候有重要含意。
简介:Inordertotheoreticallystudythebucklepropagationofsubseapipelineswithslip-onbucklearrestors,atwo-dimensionalringmodelwassetuptorepresentthepipelineandanonlinearspringmodelwasadoptedtosimulatethecontactbetweenpipeline’sinnerwallsandbetweenpipeline’souterwallandslip-onbucklearrestor’sinnerwallduringbucklepropagation.Inaddition,somereversespringsareaddedtopreventthewallofleftandrightsidesseparatingfromtheinnerwallofslip-onbucklearrestors.Consideringlargedeformationkinematicsrelationsandtheelastic-plasticconstitutiverelationofmaterial,balanceequationswereestablishedwiththeprincipleofvirtualwork.Thevariationofexternalpressurewithrespecttothecross-sectionalareaofpipelineswasanalyzed,andthelowerboundofthecrossoverpressureofslip-onbucklearrestorswascalculatedbasedonMaxwell’senergybalancemethod.Bycomparingthetheoreticalresultswithexperimentandfiniteelementnumericalsimulation,thetheoreticalmethodisprovedtobecorrectandreliable.
简介:First,Wirsching'smodel,whichiswidelyemployedinfatiguereliabilityanlysisofmarineandoffshorestructures,isanalysedsystematically.Itisfoundthattheveryimportantrandomvariable△inWirsching'smodelcannotbedirectlydeterminedfromfatigueexperimentbecauseoftheirreversibilityoffatiguetest,andinfact,whatWirschingstudiedfromtestingresultsisnot△butαofthestatisticalMiner'srule.Second,byuseofthestatisticalMiner'srule,amodifiedWirsching'smodelisproposed.Thirdlyandmoreimportantly,basedonthetwo-dimensionalprobabilisticMiner'srule,anewmodelisestablishedforfatiguereliabilityanalysisofstructuralcomponentssubjectedtospecifiedcyclicloadingofvariableamplitudeorstochastictimehistory.Intheend,anexampleispresented,fromwhichitwillbeseenthatthisnewmodelisveryconvenienttouseandfeasibletoengineeringpractice.
简介:根据一系列重要历史的地图,即,华南海岛,Nansha岛,Zhongsha岛,Xisha岛,Yongxing岛和Shidao岛的地点地图,并且太平天国的岛(在1946由中华民国的内部的部的领土的管理部门转存),并且中华民国的管理区域地图在1948出版了,包围华南海岛的击破的线在华南代表瓷器海边界它是连接与的两个,和延期,中国的陆地边界。在当稳固的线被用来代表陆地boundarya时,击破的线被用来代表水边界的那时间,通用方法在然后国际性地被认出的地图使用了。上述观察在为在华南海区域上的国际海上的定界线是有用的华南海提供瓷器海边界的历史、科学的证据。
简介:ByimplementingtheARGOprogram,alargenumberofT-Sprofilescanbeobservedintheworldocean.However,itisverydifficulttoexaminechangesofthesensitivityofthesensorsequippedattheARGOfloats,becauseitisdifficulttounderstandtheirconditionintheseaandthereliabilityofthedata.Qualitycontrolmustbedoneinordertoavoidthewrongconclusiondeducedfromthewrongdata.OneoftherealisticmethodsforqualitycontroloftheARGOdataisthecomparisonwiththelocalclimatology.HighqualityclimatologicalT-SmodelsinnorthwestPacifichavebeenbuiltbasedontheNansenbottledataandCTDdataforthequalitycontrolinNMDIS.ThemodelsareusedtochecktheARGOdatainthisareaandhavegotgoodresult.
简介:在国际标准电子海图显示信息系统(ECDIS)中,信息的表达模型是独立于数据存储的。根据空间数据处理的特点和可视化的基本技术,矢量数据的几何图形表示方法通常包括点数据表示、线数据表示和面数据表示等三种。介绍了关于点、线、面基本图元的符号化问题。
简介:DragforcesactingonSchlegel'sblackrockfishSebastesschlegeliwerestudied.AnewdragforcetransducerwasdesignedandusedtomeasurethewaterdragonSchlegel'sblackrockfishinaverticalrecirculatingflumetank.Fourteenindividualswereinvestigated,yieldingtwomeandragcoefficientsreferredtothecross-sectionalareaandvolume2/3respectivelyatwatervelocitiesrangingfrom0.3to1.0ms-1.ThedragcoefficientscanbeusedforestimatingthedragforcesactingonSebastesschlegeliinwater.
简介:Generally,thereliabilityofami-slidingandanti-overturningstabilityofanisolatedgravitycylinderinacertainworkingperiodcanbeevaluatedonlywhenthestatisticalpropertiesofshorttermstabilityaregivenfirst.Theauthorsusednumericalmethodtosimulatethestabilitystatefunctionofacylinderinshort-crestedsea,andfurthertogettheprobabiliticalcharacteristicsofthestructure’sstabilitybytimedomainanalysis.Theexternalloadsappearedinthestatefunctionsincludehorizontalwaveforce,liftforceandtherespectivemoments,andtheloadsarecorrelatedbyco-spectrum.Thenumericalmethodpresentedinthispapercanbeusednotonlytosolveshorttermreliabilityproblemdirectly,buttocalculateandanalysethelongtermreliabilityproblemaswell.Forcircularcylinders,anexampleofsimulationandanalysisisdisplayedinthispaper.
简介:BasedonthesimulationwiththeOcean-AtmosphereCoupledModelCCSMandOceanModelPOPunderthegreenhousegasemissionscenariooftheIPCCSRESA2(IPCC,2001),andontheearthcrustsubsidenceandglaciermeltingdata,therelativesealevelchangeisobtainedalongthecoastofChinainthe21stcentury.UsingtheSRTMelevationdatathesubmergenceofcoastallowlandiscalculatedundertheextremewaterlevelwitha100-yearreturnperiod.Thetotalfloodingareasare98.3×103and104.9×103km2for2050and2080,respectively.Forthethreeregionsmostvulnerabletoextremesealevelrise,i.e.,thecoastofBohaiBay,theYangtzeRiverDeltatogetherwithneighboringJiangsuProvinceandnorthernZhejiangProvince,andthePearlRiverDelta,thefloodedareasare5.0×103,64.1×103and15.3×103km2in2050and5.2×103,67.8×103and17.2×103km2in2080,respectively.
简介:Thispaperreportstheabsorption,accumulation,distributionandcycleofCu,Pb,Zn,CrandNiinS.apetala+S.caseolaris,mangrovecommunityatFutianMangroveNatureReserveofShenzhen,TheCu,Pb,Zn,CrandNicontentsinforestsoilincreasefrombottomtosurfacelayers,andthestorageofthefiveheavymetalsinthesurfacelayer(depth0-30cm)isZn>Pb>Ni>Cr>Cu.TheconcentrationabilityisS.caseolaris>S.apetala>K.candel.TheexistingaccumulationofCu,Pb,Zn,CrandNiinthecommunityis23019.63μg/m^2,23429.66μg/m^2.177870.42μg/m^2,6835.80μg/m^2,and12995.22μg/m^2,respectively.Theannualabsorptionis6592.57μg/m^2,2664.80μg/m^2,23123.56μg/m^2,853.24μg/m^2,and1990.95μg/m^2,respectively.Theannualreturnis3179.50μg/m^2,1300.65μg/m^2,7401.31μg/m^2,398.99μg/m^2,and646.20μg/m^2respectively.TheannualnetretentionaccumulationofCu,Pb,Zn,CrandNiinthecommunityis3413.07μg/m^2,1364.15μg/m^2,15722.25μg/m^2,454.25μg/m^2,and1344.75μg/m^2,respectively,TheturnoverperiodsofCu,Pb,Zn,CrandNiare8,19,15,18and21years,respectively.
简介:ThewildPorphyrayezoensiscollectedfromtheQingdaocoastwasusedtoprepareprotoplastsbyenzymedigestion.Thepurelinewasconstructedbycultivatingtheprotoplasts.The18SrDNAoftheP.yezoensispurelinewasclonedandsequenced.Sequenceanalysiswasexecutedforthissequenceandother22sequencesretrievedfromGenBank.Aphylogenetictreewasconstructedusingtheneighbor-joiningmethod.Theresultsrevealedahighdiversityof18SrDNAsequencesingenusPorphyraandtheconsiderablevariationof18SrDNAsequencesindifferentstrainsofthesamespeciesP.yezoensisandP.tenera.Significantdifferenceof18SrDNAsequencewasobservedbetweenP.yezoensisfromQingdao,China,andthetwostrainsofP.yezoensisfromJapan,butthethreestrainsofP.yezoensisformedastablecladeinthephylogenetictree.TheseresultsindicatethepossibilityofinterspeciesandintraspeciesdiscriminationofPorphyrausingthe18SrDNAsequences.
简介:18SribosomalDNA基因(18SrDNA)序列(近似在长度的1300bp)从从与线虫大批从Qingdao海岸的内部潮汐的沉积收集的免费生活的海洋的线虫提取的DNA被放大特定的教材。PCR产品被克隆,重新放大,与Rsa消化了我和Hin6我限制endonucleases和分开的inagarose胶化。在17种限制碎片长度类型之中,打1,2和6盖住61.2%,分别地,当时,克隆的14.4%and9.3%分析了留下14仅仅盖住21克隆,它说明了15.1%总数。24代表性的克隆andphylogenetically被定序由指在RDP和GenBank数据库当前可得到的那些分析了。尽管它是难的由于已知的海洋的免费生活的线虫的18SrDNA顺序数据的缺乏把这些序列分到已知的种类或类,获得的序列被分到Adenophorea的线虫。在他们之中,12个序列接近了PontonemavulgareandAdoncholaimussp,四到Daptonemaprocerus并且二(相同)到Enoplusbrevis。我们的结果证明免费生活的海洋的线虫差异能被检索的PCR决定,18SrDNA的分析定序,仅当免费生活的海洋的线虫的18SrDNA序列被积累到某程度,一个18SrDNA序列能被分到种或一个类。
简介:Comparedtotowedstreamers,ocean-bottomseismometers(OBS)obtainbothS-wavedataandricherwavefieldinformation.Inthispaper,theinducedpolarizationmethodisusedtoconductwavefieldseparationonOBSdataobtainedfromtheShenhuareaintheSouthChinaSea.AcomparisonofthechangesinP-andS-waves,andacomprehensiveanalysisofgeologicalfactorswithinthearea,enableanalysisanddescriptionoftheoccurrenceofnaturalgashydrateinthestudyarea.ResultsshowanincreaseinP-wavevelocitywhennaturalgashydrateexistsintheformation,whereastheS-wavevelocityremainsalmostconstant,asS-wavescanonlypropagatethroughtherockskeleton.Therefore,thebottom-simulatingreflection(BSR)responseoftheP-waveisbetterthanthatoftheS-waveinthefrequencyanalysisprofile.Inawide-anglesection,therefractivewaveofthehydratelayerisevidentwhenusingP-wavecomponentsbutidentificationisdifficultwithS-wavecomponents.ThisvelocitymodelillustratesthesensitivityofPandS-wavecomponentstogashydrate.TheuseofthispolarizationmethodandresultsofanalysisprovidetechnicalandtheoreticalsupportforresearchonhydratedepositsandothergeologicalfeaturesintheShenhuarea.