简介：Thepresentstudywasdesignedtoinvestigatetheanti-sepsiseffectsofphyscion8-O-β-glucopyranoside(POG)isolatedfromRumexjaponicasandexploreitspossiblepharmacologicalmechanisms.POGwasextractedfromR.japonicasbybioactivity-guidedisolationwiththeanti-sepsisagents.SurvivalanalysisinsepticmouseinducedbyLPSandheat-killedEscherichiacoliwereusedtoevaluatetheprotectiveeffectofPOG(40mg·kg~(-1),i.p.)onsepsis.CytokinesincludingTNF-α,IL-1βandIL-6inRAW264.7cellsinducedbyLPS(100ng·mL~(-1))weredeterminedbyELISA.Inaddition,theproteinsexpressionsofTLR2andTLR4weredeterminedbyWesternblottingassay.OurresultsdemonstratedthatPOG(40mg·kg~(-1),i.p.)possessedsignificantprotectiveactivityontheendotoxemicmice.ThePOGtreatment(20,40,and80μg·mL~(-1))significantlydecreasedtheTNF-α,IL-1βandIL-6inducedbyLPS(P<0.01)inaconcentration-dependentmanner.Furthermore,theTLR4andTLR2proteinswerealsodown-regulatedbyPOGat20(P<0.01),40(P<0.01),and80μg·mL~(-1)(P<0.01).ThepresentstudydemonstratedthatthePOGextractedfromR.japonicaspossessedsignificantanti-sepsiseffectonendotoxemicmice,andcanbedevelopedasanoveldrugfortreatingsepsisinthefuture.

简介：目的：建立同时测定不同产地半枝莲中野黄芩苷、黄芩苷、野黄芩素、异野黄芩素、木犀草素、芹菜素、黄芩素和汉黄芩素含量的高效液相色谱法。方法：采用AgilentZORBAXSB-C18色谱柱（250mm×4.6mm，5μm），以乙腈-0.1％甲酸水为流动相，梯度洗脱，检测波长：350nm，流速：1.0mL·min^-1，柱温：25℃。结果：半枝莲药材中野黄芩苷、黄芩苷、野黄芩素、异野黄芩素、木犀草素、芹菜素、黄芩素和汉黄芩素分别在7.23~72.32、3.28~32.76、2.35~23.53、2.54~25.37、1.77~17.65、2.26~22.57、1.95~19.48、3.52~35.24μg呈良好线性关系，相关系数r在0.9983~0.9999，平均加样回收率为98.9%~99.9%。结论：该方法简单、快速，为半枝莲的质量控制提供了参考。