简介：Alpiniapurpurata的目的Ethylacetate摘录在vitro抗氧化剂和anticancer活动为它的潜力被评估。抗氧化剂活动被1评估的方法，1-diphenyl-2-picrylhydrazyl(DPPH)免费激进的清除方法，氢氧根基活动，superoxide基清除活动，氮的氧化物基清除活动，氢过氧化物基清除活动和减少的力量活动。OAW42房间的生存能力被MTT试金评估。结果A。purpurata与一种集中依赖者方式展出了潜在的抗氧化剂活动。摘录与130.20g的IC50在第48小时显示出潜在的anticancer活动?潴??祥獥吗？

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简介：EuphorbiakansuiisacommonlyusedtraditionalChinesemedicineforthetreatmentofedema,pleuraleffusion,andasthma,etc.Accordingtothepreviousresearches,terpenoidsinE.kansuipossessvariousbiologicalactivities,e.g.,anti-virus,anti-allergy,antitumoreffects.Inthiswork,twentyfiveterpenoidswereisolatedfromE.kansui,includingthirteeningenane-andeightjatrophane-typediterpenoids(withtwonewcompounds,kansuininPandQ)andfourtriterpenoids.EighteenofthemwereanalyzedbyMTSassayforinvitroanticanceractivityinfivehumancancercelllines.Structure-activityrelationshipfor12ingenane-typediterpenoidsincolorectalcancerColo205cellswerepreliminarystudied.Significantanti-proliferationactivitieswereobservedinhumanmelanomacellsbreastcancerMDA-MB-435cellsandColo205cells.Morethanhalfoftheisolatedingenane-typediterpenoidsshowedinhibitoryactivitiesinMDA-MB-435cells.Eightingenane-andonejatrophane-typediterpenoidspossessedmuchlowerIC_(50)valuesinMDA-MB-435cellsthanpositivecontrolstaurosporine.Preliminarystructure-activityrelationshipanalysisshowedthatsubstituentonposition20wasimportantfortheactivityofingenane-typediterpenoidsinColo205cellsandsubstituentonposition3contributedmoresignificantbiologicalactivityofthecompoundsthanthatonposition5inbothMDA-MB-435andColo205cells.

简介：研究证明了许多化学成分由哌啶碱统治了，flavonoids例如lobelanidine，洛贝林，和lobelanine，从山梗莱属的植物chinensis愁相被获得了。试验性的研究和临床的应用也显示了那L。chinensis拥有很多项药理学活动(例如，利尿剂，choleretic，呼吸兴奋，反毒液，抗菌剂，和anticancer)。这篇论文集中于L的性质，化学成分，和anticancer活动。chinensis将在他们之中澄清连接，并且识别活跃anticancer混合物。这个工作为L的进一步的研究和开发用作基础。chinensis。

简介：调查的目的在里面vitro抗氧化剂活动和methanolic叶的全部的酉分的内容Nyctanthesarbor-tristisL提取。(NA)。样品在vitro抗氧化剂方法用五被测试的方法(1，1-diphenyl-2-picryl肼基清除活动(DPPH)，氢氧根激进清除的活动(-OH),氮的氧化物清除活动(没有)，superoxide激进清除的活动，并且总计抗氧化剂活动)评估在里面NA和全部的酉分的内容(Folin-Ciocalteu方法)的vitro抗氧化剂潜力。摘录看好免费激进的清除性质哪个作为IC50价值计算。methanolic摘录的结果IC50(半最大的禁止的集中)被发现是57.93?g牴慥整?慲獴愠?潢桴搠獯獥

简介：目的马尾藻类海草wightiiGreville是属于有大约200种类的Sargassaceae家庭的海洋的棕色的水藻。整个干燥植物粉末的ethanolic摘录包含了众多的phytoconstituents，包括flavonoids。学习在老鼠集中于马尾藻类海草wightii的anticancer活动。在二剂量的马尾藻类海草wightii(EESW)的ethanolic摘录铺平的方法被用来用DAL房间线在老鼠检验anticancer活动导致癌症。身体重量，可行、不能生存的肿瘤房间计数，吝啬的幸存时间，寿命的增加，和hematological参数为EESW的anticancer活动被观察。在老鼠的DAL房间的intraperitoneal接种显著地增加了的结果癌症房间计数。在在对待EESW的组癌症动物观察的癌症细胞数字的减少显示测试药在肿瘤细胞增长上有重要禁止的效果。有EESW的治疗也在肿瘤重量显示出重要减少，并且因此增加了对待DAL的老鼠的lifespan。另外，EESW管理显著地在对待DAL的老鼠恢复了hematological参数。现在的学习结果建议摘录提议的那个政府的结论提高了抗氧化剂潜力。因此，EESW拥有anticancer活动，这能从这研究被结束。

简介：ThepresentstudyinvestigatedthechemicalcompositionofethylacetateextractsfromanendophyticactinomyceteStreptomycessp.A0916anditshostPolygonumcuspidatum.Acomparativeanalysisoftheantimicrobialandantioxidantpropertiesoftheextractswasalsoconducted.32compoundsofP.cuspidatumand23compoundsofStreptomycessp.A0916wereisolatedandidentifiedbyGC/MS.Antimicrobialactivitiesoftheextractswereevaluatedusingeightmicrobialstrains(3Gram-positivebacteria,3Gram-negativebacteria,and2fungi).TheStreptomycessp.A0916extractsshowedawiderangeofantimicrobialactivitiesandpresentedgreaterantimicrobialeffectivenessthantheP.cuspidatumextracts.Theminimuminhibitoryconcentration(MIC)ofStreptomycessp.A0916extractsagainsttheampicillin-resistantstrainEnterococcusfaeciumSIIA843was32μg·mL-1.Furthermore,theextractshadgreaterantimicrobialeffectagainstGram-positivebacteriathanGram-negativebacteria.Finally,theantioxidantactivityoftheStreptomycessp.A0916extractswasequaltothatoftheP.cuspidatumextracts.Inconclusion,ourresultssuggestthattheendophyticactinomycetesofthemedicinalplantsareanimportantsourceofbioactivesubstances.

简介：Pomegranateleaf(PGL)hasadefiniteroleinregulatinglipidmetabolism.However,pharmacokineticresultsshowthemainactiveingredient,ellagicacid,inPGLhasloweroralbioavailability,suggestingthatthelipid-loweringeffectofPGLmayactthroughinhibitinglipidabsorptioninthesmallintestine.Ourresultsdemonstratedthatpomegranateleafanditsmainactiveingredients(i.e.,ellagicacid,gallicacid,pyrogallicacidandtannicacid)werecapableofinhibitingpancreaticlipaseactivityinvitro.Incomputationalmoleculardocking,thefouringredientshadgoodaffinityforpancreaticlipase.AcutelipidoverloadexperimentsshowedthatalargedosageofPGLsignificantlyreducedserumtotalcholesterol(TG)andtriglycerides(TC)levelsinadditiontoinhibitingintestinallipaseactivity,whichdemonstratedthatPGLcouldinhibitlipaseactivityandreducetheabsorptionoflipids.WealsofoundthatPGLcouldreversethereducedtight-junctionproteinexpressionduetointestinallipidoverload,promoteOccludinandClaudin4expressioninthesmallintestine,andenhancetheintestinalmucosalbarrier.Inconclusion,wedemonstratedthatPGLcaninhibitlipidabsorptionandreducebloodTGandTCbytargetingpancreaticlipase,promotingtight-junctionproteinexpressionandtherebypreventingintestinalmucosadamagefromanoverloadoflipidsintheintestine.

简介：瞄准validatory屏蔽puffer鱼以puffer鱼皮肤和肝提取的Arothronimmaculatus为抗菌剂试金被使遭到的如此的一个方法被做的抗菌剂。屏蔽试金的方法抗菌剂用标准圆盘散开方法在十连续人的病原的细菌、真菌的病原体被做。结果证实了的结果对大多数病原体的阳性的测试使用了。对在肝摘录的2.5公里和对在皮肤摘录的Vibrio霍乱的抗菌剂效果的9.8公里的Staphyloccocusaureus的最大的抗菌剂效果被报导。没有抗真菌的效果。这个实验证实那条puffer鱼的结论是抗菌剂力量的来源。

简介：试图学习在里面vivo男白化体老鼠上的Tabernaemontanadivaricata叶摘录的反煽动性的活动。方法水的煎和甲醇叶摘录为他们减少巴豆的能力被测试在在热门申请以后的鼠标耳朵的导致油的浮肿。甲醇叶摘录dose-dependently禁止了巴豆在老鼠的导致油的耳朵浮肿(ID50<500?g

简介：Theanticoagulanteffectofleechsalivawastraditionallyemployedinthetreatmentofdiabetesmellituscomplicationssuchasperipheralvascularcomplications.Thisstudywascarriedouttoexaminetheeffectofleechsalivaextract(LSE)onbloodglucoselevelsinalloxan-induceddiabeticrats.First,LSEwascollectedfromleecheswhichwerefedonaphagostimulatorysolution.Second,totalproteinconcentrationwasestimatedusingtheBradfordassay.Third,diabeticratswereinjectedsubcutaneously(sc)withLSEatdosesof500and1000μg·kg1bodyweight(bw).Otherdiabeticratswereinjectedscwithinsulinatdosesof10and20U·kg1bw.AnothergroupwasinjectedsimultaneouslywithLSE(250μg·kg1bw)andinsulin(10U·kg1bw).Fastingbloodglucose(FBG)concentrationsweremonitoredduringastudyperiodofeighthoursatregularintervals.FindingsshowedthatbothdosesofLSEresultedinasignificantandgradualdecreaseinFBGstartingfrom10%18%downfallaftertwohoursofinjectionreachingthemaximalreductionactivityof58%aftereighthours.Remarkably,LSEwassufficienttobringtheratstoanearnorm-glycemicstate.Thehighdoseofinsulininducedaseverehypoglycemicconditionafter24hofinjection.ThelowerdosewasabletodeclineFBGfor26hinratswhichbecamediabeticagainafter8h.Ontheotherhand,theconcurrentinjectionoflowdosesofLSEandinsulinproducedahypoglycemiceffectwithallratsshowingnormalFBGlevels.Takentogether,thesefindingsindicatedthatthesubcutaneousinjectionofLSEofthemedicinalMalaysianleechwasabletoprovidebetterglycemiccontrolcomparedwithinsulin.Moreover,thesynergismbetweenLSEandinsulinsuggeststhatLSEcouldbeutilizedasanadjuvantmedicationinordertoreduceinsulindosageortoachievebettercontrolofbloodglucose.

简介：Pleurotussajorcaju(P。sajorcaju)，一朵可食、无毒的蘑菇，作为抗氧化剂被评估，antitumor，反煽动性并且antihypertensive活动。P。sajorcaju是糖类，饮食的纤维，必要氨基酸，矿物质，维生素B，folic酸和类固醇的好来源。反煽动性，immunomodulatory和止痛活动水并且P的菌丝体的methanolic摘录。sajorcaju被调查(数据没被显示出)。这发现建议那P提取。sajorcaju能对煽动性、自体免疫的疾病被使用。那么，P。为它的antiarthritic活动检验的sajorcaju。植物与水和甲醇镇定、独立提取。为antiarthritic活动500和1000mg

简介：试图从粗略的摘录和从东北泰国收集的Phellinus蘑菇的十种的粗略的部分调查抗氧化剂活动和全部的酉分的内容。样品向2为他们的激进的清除活动被测试的方法，2-diphenyl-1-pricylhydrazyl(DPPH)激进分子(DPPH方法)和全部的酉分的内容(Folin-Ciocalteu方法)。一些调查摘录展出了有势力基清除的结果有从的IC50的活动(7.30来慲整?楷桴琠敨?湩漠摲牥琠?湩牣慥敳琠敨物瀠潲畤瑣癩瑩y

简介：Poriacocos(Schw.)Wolf,animportantmedicinalandfoodfungus,iswellknowninEastAsia.Duetogrowingmarketdemand,longcultivationperiod,andconsumptionofpinetrunkduringcultivation,developingalternativemethodsforproducingP.cocosand/oritsactivecomponentsisofinterest.Inthepresentstudy,theeffectsofdifferentculturemethodsonbiomassandaccumulationoffourtriterpenoidswereinvestigated.Theethanolextractoffermentedmycelium(EFM)wasorallyadministeredtorats.Urineoutputandconcentrationsofelectrolytes(Na~+,K~+,andCl~-)weremeasured.Ourresultsshowedthatmyceliagrewbetterundercontinuousshakingculturecondition(7.5gDW·L~(-1)),andhighertriterpenoidlevelswereaccumulatedintwo-stageculture(112mg·L-1,2.03%).Theoptimalstartingtimeofstaticculturefortriterpenoidyieldwas4~(th)daftershakingculture.SingleadministrationofmiddleandhighdoseofEFMsignificantlyincreasedurineoutput,Na~+andCl~-excretion,andNa~+/K~+ratio.Theseresultssuggestedthatethanolextractofculturedmyceliashowedsignificantdiureticactivityinratsandtwo-stagecultureofP.cocoscouldbeanalternativewaytoproducemyceliaandtriterpenoids.

简介：MarinesedimentsampleswerecollectedfromthecoastalareasofSouthernIndia,particularlyinKanyakumariDistrict.Twenty-eightdifferentfungalstrainswereisolated.Thescreeningoffungifrommarinesedimentwasdonetoisolateapotentfungusthatcanproducebioactivecompoundsforbiomedicalapplications.OnlythreestrainsvizTrichodermagamsiiSP4,TalaromycesflavusSP5andAspergillusoryzaeSP6werescreenedforfurtherstudies.Theintracellularbioactivecompoundswereextractedusingsolventextractionmethod.Thecrudeextractsweretestedforitsanti-microbialandanti-cancerpropertiesandanalyticallycharacterizedusingGasChromatographyMassSpectrometry(GC-MS).Allthethreeextractswereactive,buttheextractfromT.flavusSP5wasfoundtobemoreactiveagainstvarioushumanpathogens,viz.,PseudomonasaeruginosaATCC27853(17.8±0.1),EscherichiacoliATCC52922(18.3±0.3),andCandidatropicalisATCC750(17.7±0.4).ItalsoexhibitedcytotoxicactivityagainstHEp2carcinomacelllinewiththeLC_(50)valueof25.7μg·mL~(-1).TheGC-MSdatarevealedthepresenceofeffectivebioactivecompounds.TheseresultsrevealedthattheextractfromisolatedfungusT.flavusSP5actedasapotentantimicrobial,antifungal,andanticanceragent,providingbasicinformationonthepotencyofmarinefungitowardsbiomedicalapplications;furtherinvestigationmayleadtothedevelopmentofnovelanticancerdrugs.

简介：在忍冬装饰用的梨树花的di-O-caffeoylquinic酸(diCQAs)的三种类型的抗菌剂活动，繁体中文药(TCM)，在杆菌shigae上，生长被microcalorimetry调查并且比较。diCQAs的三种类型是3，4-di-O-caffeoylquinic酸(3，4-diCQA)，3，5-di-O-caffeoylquinic酸(3，5-diCQA)，并且4，5-di-O-caffeoylquinic酸(4，5-diCQA)。新陈代谢的力量时间上的三diCQAs的效果的质、量的信息弄弯的一些，生长率常数k，最大的热产量力量下午，和产生预定tG，全部的热产量Qt，和生长禁止的比率我B。shigae是计算的。根据一个thermo运动的模型，k，下午，Qt，我和c的相应量的关系被建立。另外，这些药(IC50)的一半禁止的集中被定量分析获得。基于数量活动关系和IC50价值，禁止的活动的顺序是3，5-diCQA>4，5-diCQA>3，4-diCQA。结果说明在C-5的caffeoyl酉旨组是为细菌的房间有一种更高的亲密关系的主要的组的可能性，并且二caffeoyl酉旨的intramolecular距离组织，这也在diCQAs的抗菌剂活动有重要影响。

简介：AnovelPleurotusnebrodensispolysaccharide(PN-S)waspurifiedandcharacterized,anditsimmune-stimulatingactivitywasevaluatedinRAW264.7macrophages.PN-SinducedtheproliferationofRAW264.7cellsinadose-dependentmanner,asdeterminedbytheMTTassay.AfterexposuretoPN-S,thephagocytosisofthemacrophageswassignificantlyimproved,withremarkablechangesinmorphologybeingobserved.FlowcytometricanalysisdemonstratedthatPN-SpromotedRAW264.7cellstoprogressthroughSandG2/Mphases.PN-Streatmentenhancedtheproductionsofinterleukin-6(IL-6),nitricoxide(NO),interferongamma(INF-γ),andtumornecrosisfactor-α(TNF-α)inthemacrophages,withup-regulationofmRNAexpressionsofinterleukin-6(IL-6),induciblenitricoxidesynthase(iNOS),interferongamma(INF-γ)andtumornecrosisfactor-α(TNF-α)beingobservedinadose-dependentmanner,asmeasuredbyqRT-PCR.Inconclusion,theseresultssuggestthatthepurifiedPN-Scanimproveimmunitybyactivatingmacrophages.

简介：AIM:JS-38(mitothiolore),asyntheticversionofametaboliteisolatedfromXenorhabdussp.,wasevaluatedforitsanti-tumorandwhitebloodcell(WBC)elevatingactivities.METHOD:Theseanti-proliferativeactivitieswereassessedinvitrousingapaneloftencelllines.Theanti-tumoractivitiesweretestedinvivousingB16allograftmousemodelsandxenograftmodelsofA549humanlungcarcinomaandQGYhumanhepatomainnudemice.Theanti-tumorinteractionsofJS-38andcyclophosphamide(CTX)or5-fluorouracil(5-Fu)werestudiedinaS180sarcomamodelinICRmice.SpecificstimulatoryeffectsweredeterminedonperipheralneutrophilsinnormalandCTX-and5-Fu-inducedneutropenicmice.RESULTS:TheIC50valuesrangedfrom0.1to2.0μmol·L-1.JS-38(1μmol·L-1)causedanincreaseinA549tumorcellapoptosis.Multi-dailygavageofJS-38(15,30,and60mg?kg-1?d-1)inhibitedinvivotumorprogressionwithoutasignificanteffectonbodyweight.JS-38additivelyenhancedtheinvivoanti-tumoreffectsofCTXor5-Fu.JS-38increasedperipheralneutrophilcountsandneutrophilratesinnormalBALB/cmicealmostaseffectivelyasgranulocytecolony-stimulatingfactor(G-CSF).InmicewithneutropeniainducedbyCTXor5-Fu,JS-38rapidlyrestoredneutrophilcounts.CONCLUSION:TheseresultssuggestthatJS-38hasanti-tumoractivity,andalsohastheabilitytoincreaseperipheralbloodneutrophils.

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