简介：Inordertocreateanewmimicofglutathioneperoxidase(GPx),bioimprintingwasusedtogenerategluta-thione(GSH)bindingsiteandchemicalmodificationwasusedtoincorporatecatalyticgroupselenocystine(Sec).Humanserumalbumin(HSA)andS-substituteddinitrophenylglutathione(GSH-S-DNP)werechosenastheimprintedmatrixandimprintingtemplate,respectively,togenerateaGSH-imprintedprotein(GSH-HSA)bybioimprinting.SecwasincorporatedintotheGSH-HSAbychemicalmodificationtogiveanewGPxmimic(Se-GSH-HSA).Se-GSH-HSAdisplayedconsiderablyhigherGPxactivitythannon-printedHSA(Se-HSA).TheenzymicpropertiesandkineticsofSe-GSH-HSAwerestudied.Moreover,Se-GSH-HSAwasconfirmedtohavestrongerantioxidantabilitytoprotectmitochondriaagainstoxidativedamagewithferroussulfate/ascorbate-inducedmitochondriadamagemodel,indicatingthatSe-GSH-HSAhaspotentialapplicationinmedicine.

简介：Alpiniapurpurata的目的Ethylacetate摘录在vitro抗氧化剂和anticancer活动为它的潜力被评估。抗氧化剂活动被1评估的方法，1-diphenyl-2-picrylhydrazyl(DPPH)免费激进的清除方法，氢氧根基活动，superoxide基清除活动，氮的氧化物基清除活动，氢过氧化物基清除活动和减少的力量活动。OAW42房间的生存能力被MTT试金评估。结果A。purpurata与一种集中依赖者方式展出了潜在的抗氧化剂活动。摘录与130.20g的IC50在第48小时显示出潜在的anticancer活动?潴??祥獥吗？

简介：一系列新与酉分的抗氧化剂消炎痛结合为提高的antiinflammatory被综合活动以及减少的ulcerogenic力量。都结合，这被发现很在vitro的有势力抗氧化剂。他们能显著地禁止类脂化合物peroxidation，当11b11e和13b13e能也与DPPH交往时。然而这些对巴豆油结合显示出的小抑制导致的老鼠耳朵胀大。

简介：超声的抽取(UE)为竹子叶多糖(BLP)的抽取被采用。包括抽取时间，超声的力量和固体/液体的比率的UE过程的有影响的参数被直角的实验优化。DEAE纤维素列层析被使用净化BLP然后BLP的激进的清除活动也被评估。最佳的抽取条件是：15min，300W的超声的力量，和1:15的固体/液体的比率的抽取时间。四种多糖被DEAE纤维素列层析获得；清除的最大的superoxide激进分子BLP的率(20.4%)低于维生素C的(VC，控制)并且清除的氢氧根激进分子率(50%)等价于VC的。

简介：BackgroundFlavonoidsfromfruits,vegetablesandplantshavebeenwidelystudiedontheireffectsofimprovinglipidemia,anti-inflammation,anti-plateletaggregationandanti-oxidativeactivities.However,wedon'tknowifflavonoidsfromrapebeepollenhavethesameeffectsinpatientswithdyslipidemia(DL),type2diabetesmellitus(T2DM),andcerebralinfarction(CI).MethodsThestudyinvolvedwithpatientsselectedanddividedinto4groups,30casesofDL,ofT2DM,ofCIandhealthcontrol(HC)foreach.Fortymgflavonoidsfromrapebeepollenadministratedorallytwiceadayfortwomonths.Bloodsugar(BG),totalcholesterol(TC),triglyceride(TG),highdensitylipoprotein-cholesterol(HDL-C),lowdensitylipoprotein-cholesterol(LDL-C),whiteblood-cellcounts(WBC),plateletcounts(PLT),high-sensitivityC-reactiveprotein(hsCRP)andmalondialdehyde(MDA)wereanalyzedinfourgroupsbeforeandaftertakingflavonoidsfortwomonths.Results(1)BasiclevelsofTC,BG,WBC,PLTandhsCRPingroupsofT2DM,CI,andTGandMDAingroupsofDL,T2DMandCIweresignificantlyhigherthanthoseinHC(P<0.05-0.001).(2)ThelevelsofTG,WBC,PLT,hsCRPandMDAweresignificantlydecreased,whileHDL-Cwasremarkablyincreasedaftertakingflavonoidsfortwomonths(P<0.05-0.001)ingroupofDL,T2MD,andCI.TheseindexeswerenotdifferentinthegroupofHCaftertakingflavonoids(P>0.05).ConclusionsTheresultscouldindicatemetabolicdisturbance,inflammation,andhighoxidativestressinpatientswithdyslipidemisa,type2diabetesmellitus,andcerebralinfarction.Theflavonoidsfromrapebeepollenmayhaveeffectsofantioxidantactivity,improvinglipids,andanti-inflammationonthesepatients.

简介：影响在米饭变化在光合作用和抗氧化剂能力上浸没Swarna和Swarna-Sub1与或没有Sub1QTL在控制下面被评估，模仿完全浸没并且随后的重新通风。叶光合的率和有气孔的传导力在前进期间在两个变化减少了当与控制相比植物，而是重要品种的差别在1d以后被观察，浸没浸没。浸没也改变了PSII活动，在Fo的价值在减少思考了叶绿素的Fm和Fv/Fm和降级，更在里面Swarna比在Swarna-Sub1。在期间早浸没时期，superoxidedismutase(草皮)的活动，ascorbateperoxidase(APX)，dehydroascorbatereductase(DHAR)，对反应的氧种类的谷胱甘肽reductase(GR)在两个变化被增加。然而，与进步浸没时期(在7d以后)，草皮，过氧化氢酶(猫)，APX，guaiacolperoxidase(GPX)，GR和DHAR的活动衰退了，更在里面Swarna比在Swarna-Sub1。在重新通风期间，Swarna-Sub1显示出上面的抗氧化剂酶然而并非在Swarna的重要增加。Swarna-Sub1改进光合的活动，显示出与Swarna在下面相比的更高光合的率浸没并且随后的重新通风因为叶绿素，更高有气孔的传导力，和与从氧化损坏的更好的抗氧化剂保护一起的有效PSII活动的更少的降级。

简介：尽管氧化是最普通生物并且精力生产反应，因为象自由激进分子和过氧化物那样的氧化的产品损坏细胞的部件，氧化应力对房间有害，引起几疾病。在DNA的损坏为癌症形成和前进负责。然而，几酶象superoxidedismutase那样，过氧化氢酶，谷胱甘肽peroxidase，谷胱甘肽reductase，是的谷胱甘肽S-transferase等等行为影响氧化应力的抗氧化剂。在这些酶的多型性应当与DNA损坏被联系，随后，个人癌症冒险危险性。这篇评论文章试图进一步阐明在由在癌症病人有关表示层次和抗氧化剂酶的基因多型性总结一些重要学习的调查结果的抗氧化剂酶和癌症之间的关系。

简介：Objective:TheaimofthepresentstudywastoinvestigateantioxidantandtheanticancerigenactivityofamethanolextractfromArtemisiaprincepsvar.orientalis(APME),awell-knowntraditionalherbalmedicineinAsia,inhepatocellularcancercells.Methods:ToevaluatetheantioxidantactivityofAPME,reactiveoxygenspecies(ROS)andtheantioxidantenzymes,superoxidedismutase(SOD)andcatalasewereinvestigatedinHepG2cellsexposedtoAPME(5,100,and200μg/mL)for72h.Then,toevaluatetheanticanceractivityofAPME,weinvestigatedtheproliferationandapoptosisinductionofHepG2andHep3BcellsexposedtoAPME(1-200μg/mL)for24,48,and72h.Results:APMEdose-dependentlyreducedthegenerationofROSinthepresenceofH2O2comparedwithcontrolcells.Furthermore,itincreasedcatalaseandSODactivity.Moreover,APMEinhibitedcellproliferationinadose-andtime-dependentmanner,butatconcentrationslowerthan100μg/mL,theinhibitionwaslessdose-dependentthantime-dependent.HepG2andHep3Bcellsexposedto5,100,and200μg/mLAPMEfor72hunderwentcellcyclearrestandapoptosis.ExposuretoAPMEresultedinasignificantincreaseinthenumberofcellsinG1phaseandadecreaseintheG2/Mphasecellpopulation.Inaddition,APMEinducedP53expressionofHepG2cellsinadose-dependentmanner,andplayedaroleinthedownregulationofBcl-2andupregulationofBaxinbothHepG2andHep3Bcells.Conclusions:TheseresultsindicatethepotentialroleofAPMEasanantioxidantandanticancerigenagentinhepatocarcinomacelllines.

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简介：AbstractOxidative stress is caused by the imbalance between the generation of free radicals/reactive oxygen species (ROS) and the antioxidant defense systems, which can activate various transcription factors and affect their transcriptional pathways. Oxidative stress plays an important role in the occurrence and development of leukemia and is closely related to the treatment and prognosis of leukemia. The standard chemotherapy strategies for the pre-treatment of leukemia have many drawbacks. Hence, the usage of antioxidants and oxidants in the treatment of leukemia is being explored and has been preliminarily applied. This article reviews the research progress of oxidative stress and leukemia. In addition, the application of antioxidants treatment in leukemia has been summarized.

简介：三新diarylheptanoids，即，钠(5S,2E)-1,7-bis(4-hydroxyphenyl)-1-hydroxy-2-hepten-5-sulfonate(1)，钠(5R,2E)-1,7-bis(4-hydroxyphenyl)-1-hydroxy-2-hepten-5-sulfonate(2)和3,5-diacetoxy-1-(3-methoxy-4,5-dihydroxyphenyl)-7-(4-hydroxy-3-methoxyphenyl)heptane(3)从姜officinale的根茎被孤立。新混合物的结构根据分光镜的方法被阐明。3的抗氧化剂活动在包含DPPH自由激进分子和超级氧化物阴离子激进分子的vitro模型旁边是assayed。

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简介：Inordertoelucidatetheroleofantioxidantresponsesinsalinitytoleranceinricegenotypesundersaltstress,experimentswereconductedusingfourricevarieties,includingsalt-sensitiveBRRIdhan28andthreesalt-tolerantvarietiesBRRIdhan47,BINAdhan8andBINAdhan10.Thirty-day-oldriceseedlingsweretransplantedintopots.Attheactivetilleringstage(35daftertransplanting),plantswereexposedtodifferentsalinitylevels(0,20,40and60mmol/LNaCl).Saltstresscausedasignificantreductioningrowthforallthericegenotypes.Growthreductionwashigherinthesalt-sensitivegenotypethaninthesalt-tolerantones,andBINAdhan10showedhighersalttoleranceinallmeasuredphysiologicalparameters.ThereductioninshootandrootbiomasswasfoundtobeminimalinBINAdhan10.ChlorophyllcontentsignificantlydecreasedundersaltstressexceptforBINAdhan10.Prolinecontentsignificantlyincreasedinsalt-tolerantricegenotypeswithincreasedsaltconcentration,andthehighestprolinecontentwasobtainedfromBINAdhan10undersaltstress.Catalaseandascorbateperoxidaseactivitiessignificantlydecreasedinsalt-sensitivegenotypewhereassignificantlyincreasedinsalt-tolerantoneswithincreasingsaltconcentration.However,saltstresssignificantlydecreasedguaiacolperoxidaseactivityinallthericegenotypesirrespectiveofsalttolerance.K+/Na+ratioalsosignificantlydecreasedinshootsandrootsofallthericegenotypes.Thesalt-tolerantgenotypeBINAdhan10maintainedhigherlevelsofchlorophyllandprolinecontentsaswellascatalaseandascorbateperoxidaseactivitiesundersaltstress,thus,thismightbetheunderlyingmechanismforsalttolerance.

简介：Marineorganismsconstituteunlimitedresourceofbioactivesubstancesduetotheirhighbiodiversityandrepresentavaluablesourceofnewcompounds.Thisstudyoptimizedthealkali-extractionconditionsandantioxidantactivitiesofsolublepolysaccharidesfromthebodywallofSipunculusnudus.Theeffectsofsolid-liquidratio,extractionduration,extractiontemperature,andalkaliconcentrationontheyieldofS.nuduspolysaccharides(SNP)wereexamined,accordingtowhichtheoptimalcombinationofextractionparameterswasobtainedbyanorthogonaltest.TherelativeinfluencingimportanceofdifferentextractionparametersontheyieldofSNPfollowedtheorderassolid-liquidratio>extractiontemperature>alkaliconcentration>extractionduration.Thehighestextractionyield,1.98%,wasachievedunderanoptimalextractioncondition:temperature60℃,solid-liquidratio1:6gmL?1,duration5h,andalkali(NaOH)massfraction6%.TheinvitroantioxidantactivitiesexaminationshowedthatextractedSNPunderthisoptimizedconditionhadstrongpowerinreducingcertainhydroxylandsuperoxideradicalscavengingabilities.ThepromisingresultsshowedthatextractedSNPcouldbeapotentnaturalantioxidant.

简介：联系精索静脉曲张的男不孕古典主义地用外科被管理了或帮助了繁殖技术。与在联系精索静脉曲张的不孕作为一个pathophysiological因素增加氧化应力的证据，医药治疗特别抗氧化剂可能与更低的风险成为一种治疗选择。我们在归因于精索静脉曲张的男不孕的管理在各种各样的医药代理人的角色上考察了存在文学。医药治疗典型地与外科对药治疗作为辅助治疗与安慰剂，药对手术的(b)比较，和药的(c)比较处于象二药或一药的(a)比较那样的三种不同状况被评估独自一个。由于数据的异质和进行得好的研究的缺乏，有不够的数据与联系精索静脉曲张的不孕为人推荐医药治疗的平淡的使用，外科仍然是选择的处理。怀孕和实时出生率通常没在精子参数在大多数研究和仅仅的改进被报导或抗氧化剂能力是不够的支持它的平淡的使用。抗氧化剂治疗由于它的理论利益，从现出症状之前的潜的研究的数据，和主要副作用的缺乏是一种潜在的选择。有在精索静脉曲张的外科的修理以后的抗氧化剂的辅助治疗可以改进结果并且是为进一步的研究的一个潜在的区域。