简介：MolecularmechanismsoftheKru?ppel-likefamilyoftranscriptionfactors(KLFs)havebeenstudiedmoreinproliferatingcellsthaninpost-mitoticcellssuchasneurons.WerecentlyfoundthatKLFsregulateintrinsicaxongrowthabilityincentralnervoussystem(CNS)neuronsincludingretinalganglioncells,andhippocampalandcorticalneurons.Withatleast15of17KLFfamilymembersexpressedinneuronsandatleast5structurallyuniquesubfamilies,itisimportanttodeterminehowthiscomplexfamilyfunctionsinneuronstoregulatetheintricategeneticprogramsofaxongrowthandregeneration.BycharacterizingthemolecularmechanismsoftheKLFfamilyinthenervoussystem,includingbindingpartnersandgenetargets,andcomparingthemtodefinedmechanismsdefinedoutsidethenervoussystem,wemaybetterunderstandhowKLFsregulateneuritegrowthandaxonregeneration.

简介：ThedefiningneuropathologicalfeatureofParkinson’sdisease(PD)isthelossofnigrostriataldopaminergic(DA)projections.Thisresultsinabiochemicalreductionofstriataldopaminelevelsandmovementdisorders,suchasatremoratrest,rigidityofthelimbs,bradykinesia,andposturalinstability(Kimetal.,2011;Kimetal.,2012;BurkeandO’Malley,2013;Leemetal.,2014;Nametal.,2014).BecausetheetiologyofPDisnotfullyunderstood,itcannotguidethedevelopmentofknowledge-basedtargetedtherapeutics.

简介：OBJECTIVE:Toinvestigatethefactorsassociatedwithsensoryandmotorrecoveryaftertherepairofupperlimbperipheralnerveinjuries.DATASOURCES:TheonlinePubMeddatabasewassearchedforEnglisharticlesdescribingoutcomesaftertherepairofmedian,ulnar,radial,anddigitalnerveinjuriesinhumanswithapublicationdatebetween1January1990and16February2011.STUDYSELECTION:Thefollowingtypesofarticlewereselected:(1)clinicaltrialsdescribingtherepairofmedian,ulnar,radial,anddigitalnerveinjuriespublishedinEnglish;and(2)studiesthatreportedsufficientpatientinformation,includingage,mechanismofinjury,nerveinjured,injurylocation,defectlength,repairtime,repairmethod,andrepairmaterials.SPSS13.0softwarewasusedtoperformunivariateandmultivariatelogisticregressionanalysesandtoinvestigatethepatientandinterventionfactorsassociatedwithoutcomes.MAINOUTCOMEMEASURES:SensoryfunctionwasassessedusingtheMackinnon-Dellonscaleandmotorfunctionwasassessedusingthemanualmuscletest.SatisfactorymotorrecoverywasdefinedasgradeM4orM5,andsatisfactorysensoryrecoverywasdefinedasgradeS3+orS4.RESULTS:Seventy-onearticleswereincludedinthisstudy.Univariateandmultivariatelogisticregressionanalysesshowedthatrepairtime,repairmaterials,andnerveinjuredwereindependentpredictorsofoutcomeaftertherepairofnerveinjuries(P<0.05),andthatthenerveinjuredwasthemainfactoraffectingtherateofgoodtoexcellentrecovery.CONCLUSION:Predictorsofoutcomeaftertherepairofperipheralnerveinjuriesincludeage,gender,repairtime,repairmaterials,nerveinjured,defectlength,anddurationoffollow-up.

简介：Previousstudieshavedemonstratedthatdoublecortin-positiveimmatureneuronsexistpredominantlyinthesuperficiallayerofthecerebralcortexofadultmammalssuchasguineapigs,andtheseneuronsexhibitveryweakpropertiesofself-proliferationduringadulthoodunderphysiologicalconditions.Toverifywhetherenvironmentalenrichmenthasanimpactontheproliferationandmaturationoftheseimmatureneuronsintheprefrontalcortexofadultguineapigs,healthyadultguineapigsweresubjectedtoshort-termenvironmentalenrichment.Animalswereallowedtoplaywithvariouscognitiveandphysicalstimulatingobjectsoveraperiodof2weeks,twiceperday,for60minuteseach.Immunofluorescencestainingresultsindicatedthatthenumberofdoublecortin-positivecellsinlayerIIoftheprefrontalcortexwassignificantlyincreasedaftershort-termenvironmentalenrichmentexposure.Inaddition,thesedoublecortin-positivecellsco-expressed5-bromo-2-deoxyuridine(amarkerofcellproliferation),c-Fos(amarkerofcellviability)andNeuN(amarkerofmatureneurons).Experimentalfindingsshowedthatshort-termenvironmentalenrichmentcaninduceproliferation,activationandmaturationofdoublecortin-positivecellsinlayerIIoftheprefrontalcortexofadultguineapigs.