简介：AbstractPlatelet-rich fibrin (PRF) is a second-generation platelet concentrate obtained from autologous blood. PRF is composed of abundant platelets, leucocytes, and a high concentration of various growth factors and fibrinogen. The composition and three-dimensional structure of PRF enable it to effectively make cells migrate and proliferate, playing an important role in tissue repair. Furthermore, the easy preparation and low cost of PRF make it a good treatment option. Numerous articles have been published about the application of PRF in clinical practice, however, the application of PRF in dermatology has not been comprehensively reviewed. The objective of this review article was to discuss various applications of PRF in dermatology, including healing chronic wounds, treating androgenic alopecia, skin rejuvenation, autologous fat transplantation, and treating vitiligo. PRF is a promising dermatologic treatment, but lacks a standardized protocol regarding its methods of attainment and use, which needs more investigations.

简介：AbstractObjective:Platelet-rich plasma (PRP) releases growth factors upon activation, which in turn accelerates healing and regeneration of the target tissue. However, PRP composition may vary according to the patient’s demographics, and wider applications of PRP warrant product standardization. The current study aimed to examine variables influencing the platelet-derived growth factor BB (PDGF-BB) concentration in PRP.Methods:This observational study was conducted in the Department of Pathology and Dentistry at Swami Rama Himalayan University, a tertiary care hospital in northern India from December 2016 to November 2017. PRP was prepared from 40 mL of whole blood from 35 individuals (22 women, 13 men). Platelet counts, platelet indices (platelet distribution width, mean platelet volume) and PDGF-BB levels were measured, and platelet yield, platelet dose, and growth factor dose in PRP were also calculated. All parameters were analyzed using Pearson’s correlation coefficient. The association between PDGF-BB and PRP platelet count was evaluated using logistic regression. This study was approved by the Ethics Committee of Swami Rama Himalayan University (SRHU/HIMS/ETHICS/2016/103) on September 7, 2016.Results:The mean platelet count, PDGF-BB concentration, platelet yield, platelet dose, and growth factor dose in PRP were 1317×109/L, 30±9.89ng/mL, 71.62±28.34%, 6.5±3.5×109, and 159.62±52.39ng/mL, respectively. Linear regression analysis indicated that PRP platelet counts were a good predictor for PGDF-BB (P<0.05; adjusted R2=0.96. PRP platelet count was significantly positively correlated with PDGF-BB concentration (r=0.74, P<0.001), platelet yield (r=0.80, P<0.001), platelet dose (r=1, P<0.001), and growth factor dose (r=0.74, P<0.001).Conclusions:PRP has wide clinical applications associated with its healing and regenerative properties, and both the quality and quantity of PRP thus need to be standardized as per the requirements. Evaluating variables affecting PRP will thus aid pathologists and clinical practitioners.

简介：AbstractObjective:Androgenetic alopecia (AA) is a progressive hair loss disorder mediated by systemic androgens and genetic factors. A variety of AA treatments have been investigated. Currently, there is emerging evidence and growing interest in the use of platelet-rich plasma (PRP) for AA. This study describes a single-center experience using PRP to treat AA in women.Methods:A retrospective observational study design was employed. The study cohort comprised 20 women >18 years of age who were diagnosed with AA. PRP was prepared using a commercially available PRP kit. Each patient received six PRP treatment sessions at 4-week intervals. The severity of alopecia tool (SALT) scoring system was used to measure the severity of alopecia, and a paired t-test was used to calculate significance levels.Results:The mean pre-intervention and post-intervention total SALT score was 27.5 ± 6.35 and 9.41 ± 3.71, respectively. The difference in the total mean SALT score was 18.33 ± 1.64 and the effect size was 3.52. The scalp area with the largest effect size was the vertex (Cohen d= 2.53). The effect size was similar across other scalp areas (Cohen d= 1.91-2.09). There were no serious adverse effects of the treatment; only mild transient adverse effects were reported.Conclusion:The present study demonstrated the efficacy and safety of PRP injections in treating AA in women. However, these findings require confirmation in well-designed studies using standardized treatment protocols and evaluation methods.

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简介：为阐明β榄香烯的抗血小板作用，本文分别采用比浊法和放免法测定大鼠连续ｉｐ７ｄβ榄香烯乳６．２５～１２．５ｍｇ·ｋｇ－１·ｄ－１后对血小板聚集、血浆ｋｅｔｏＰＧＦ１α和ＴＸＢ２水平的影响。结果表明，本品分别使凝血酶、花生四烯酸和ＡＤＰ诱导血小板最大聚集率下降３８．３％～４２．６％，１４．７％～１９．１％和７．２％～１０．８％，血浆ＴＸＢ２从８８ｎｇ·Ｌ－１下降至７２ｎｇ·Ｌ－１，ｋｅｔｏＰＧＦ１α从１７．５ｎｇ·Ｌ－１增加至２０．９ｎｇ·Ｌ－１。提示ＴＸＢ２降低和ｋｅｔｏＰＧＦ１α值增加是其抑制血小板聚集的作用机制之一。

简介：AIM:Toinvestigatethediagnosticaccuracyofthemeanplateletvolumeandplateletdistributionwidthinacuteappendicitis.METHODS:Thisretrospective,case-controlledstudycompared295patientswithacuteappendicitis(Group?Ⅰ),100patientswithotherintra-abdominalinfections(GroupⅡ),and100healthyindividuals(GroupⅢ)betweenJanuary2012andJanuary2013.Theage,gender,andwhitebloodcellcount,neutrophilpercentage,meanplateletvolume,andplateletdistributionwidthvaluesfrombloodsampleswerecomparedamongthegroups.StatisticalanalyseswereperformedusingSPSSforWindows21.0software.Inaddition,thesensitivity,specificity,positiveandnegativepredictivevaluesandlikelihoodratios,anddiagnosticaccuracywerecalculated.RESULTS:Themeanagesofpatientswere29.9±12.0yearsforGroup?Ⅰ,31.5±14.0yearsforGroupⅡ,and30.4±13.0yearsforGroupⅢ.Demographicfeaturessuchasageandgenderwerenotsignificantlydifferentamongthegroups.Whitebloodcellcount,neutrophilpercentageandplateletdistributionwidthweresignificantlyhigherinGroup?Ⅰ?comparedtogroupsⅡandⅢ(P<0.05).Diagnostically,thesensitivity,specificityanddiagnosticaccuracywere73.1%,94.0%,and78%forwhitebloodcellcount,70.0%,96.0%,and76.0%forneutrophilpercentage,29.5%,49.0%,and34.0%formeanplateletvolume,and97.1%,93.0%,and96.0%forplateletdistributionwidth,respectively.ThehighestdiagnosticaccuracydetectedwasforplateletdistributionwidthbetweenGroup?Ⅰ?andGroupⅢ(P<0.01).CONCLUSION:Plateletdistributionwidthanalysiscanbeusedfordiagnosisofacuteappendicitiswithoutrequiringadditionaltests,thusreducingthecostandlossoftime.

简介：ObjectiveTrauma在在沙特阿拉伯的年轻年龄组之中是死亡和病态的领先的原因并且开发国家。这研究试图为临床的严厉作为一个潜在的预言者与创伤的大脑损害(TBI)在孩子评估血小板计数的秋天，outcome.MethodsTotally，有TBI的74个病人从开始2008年1月到目的进入我们的医院的小儿科的特别护理单位(PICU)2010年3月(27个月)。基线注册标准是很长时间12年，在在损伤事件以后的4个小时以内的承认，和缩短的损害规模(AIS)(或)，3.1；相对风险(RR)，2.15。接收装置操作特征(巨鸟)曲线和Youden索引证明PFP的最佳截止点在51.5%.ConclusionPFP与TBI的严厉被增加，它能也为它的结果作为一个重要独立预言因素被拿。

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简介：AbstractBackground:Previous studies have shown that inflammation plays an important role in intracranial atherosclerotic stenosis (ICAS). The platelet-to-lymphocyte ratio (PLR) has recently emerged as a potential inflammatory biomarker. This study aimed to explore the association of the PLR with ICAS in a Chinese Han population.Methods:A total of 2134 participants (518 with ICAS, 1616 without ICAS) were enrolled in this study. ICAS was defined as atherosclerotic stenosis >50% or the occlusion of several main intracranial arteries. Multivariable logistic regression analyses were used to assess the association of the PLR with ICAS. Additional subgroup analyses were performed according to age (<60 vs. ≥ 60 years) and acute ischemic stroke.Results:Multivariate regression analysis showed that a high PLR was associated with a higher risk of ICAS in all participants (P < 0.001). Compared with the lowest quartile, the fourth PLR quartile was significantly associated with ICAS (OR 1.705, 95% confidence interval 1.278–2.275, P < 0.001). In the subgroups stratified by age, an association between the PLR and ICAS was found in the late-life group (P < 0.001), but not in the mid-life group (P = 0.650). In the subgroups stratified by acute ischemic stroke, the relationship between an elevated PLR and a higher risk of ICAS remained unchanged (stroke group, P < 0.001; non-stroke group, P = 0.027).Conclusions:An elevated PLR was associated with a higher risk of ICAS in a Chinese Han population. The PLR might serve as a potential biomarker for ICAS in the elderly population.

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简介：AbstractThe aim of this study was to evaluate the performance of an assay using dried plasma spot (DPS) and dried blood spot (DBS) samples for the serological detection of anti-hepatitis C virus (HCV) antibodies. Between January and July 2019, plasma, DPS and DBS specimens were collected from individuals at high-risk for HCV infection. Samples were tested for anti-HCV by ELISA, and the performance of DPS and DBS specimens was examined using results from the plasma testing, as the standard. Blood samples were collected from 329 persons, including 129 men who have sex with men and 200 intravenous drug users. Results from the plasma testing indicated that 118 samples (59.0%) were HCV positive. Data from the DPS sample testing showed sensitivity as 99.2% (95% confidence interval [CI]: 0.95-1.00) and specificity as 100% (95% CI: 0.98-1.00) for HCV detection, with Kappa of 99.3% (95% CI: 0.98-1.00) while in DBS sample testing the sensitivity as 98.3% (95% CI: 0.93-1.00) and specificity as 100% (95% CI: 0.98-1.00), with Kappa of 98.7% (95% CI: 0.97-1.00), respectively. Spearman’s correlation coefficients for the comparisons between plasma and DPS specimen, plasma and DBS specimens, DPS and DBS specimens were 0.857, 0.750, and 0.739, respectively. Compared with the results in plasma, 1 sample was not detected using the DPS specimens, and 2 samples were failed for the positive detection, using the DBS specimens. Both DPS and DBS samples were promising alternatives to plasma, for the detection of anti-HCV antibodies.

简介：BACKGROUND:ThepharmacologicalactionoftraditionalChinesemedicinecompoundisthecomprehensiveeffectofthevariousingredients,andtheinteractionsofvariousingredientsarecloselycorrelatedwiththefinaleffect.InordertorevealthecompatibilitymechanismofBHD'sprescriptionintreatingandpreventingischemiccerebrovasculardisease,weneededexploretheeffectandrelationofingredientsintheprescription.OBJECTIVE:ToobservetheeffectofBuyangHuanwudecoction(BHD)andAstragalusmongholicusontheactivityofplateletactivatingfactorreceptor(PAFR)intheplateletofrabbitsinvitro,andinvestigatethemechanismofAstragalusmongholicus.DESIGN:Adecomposedrecipesstudy.SETTING:GuangzhouUniversityofTraditionalChineseMedicine.MATERIALS:FiveNewZealandrabbits,weighing2-3kg,bothsexes,wereused.BHDwascomposedofShengHuangQi120g,DangGuiWei6g,ChiShao4.5g,ChuanXiong3g,DiLong3g,TaoRen3g,HongHua3g.TheprescriptionforactivatingbloodcirculationconsistedofDangGuiWei6g,ChiShao4.5g,ChuanXiong3g,DiLong3g,TaoRen3gandHongHua3g.Theprescriptionforinvigoratingqiconsistedof120gShengHuangQi.ThepreparedherbalpieceswerepurchasedfromthetraditionalChinesemedicineDispensaryofFoshanSecondPeople'sHospital,andappraisedbyProfessorXufromScienceofChineseMateriaMedicaCollege,GuangzhouUniversityofTraditionalChineseMedicine.3H-PAFwassuppliedbyAmershamCo.,Ltd.(specificactivity:6.475TBq/mmol;batchnumber:200402);PAFstandardbyBiomolCo.,Ltd.(batchnumber:P1318V).METHODS:TheexperimentswerecarriedoutintheLaboratoryofNuclearMedicine,GuangzhouUniversityofTraditionalChineseMedicinefromSeptembertoDecember2004.①InjectionsofBHD,prescriptionsforactivatingbloodcirculationandinvigoratingqiwerepreparedbythedecoctionandalcoholsedimentationtechnique.Rabbitcommoncarotidarteryblood(40mL)wasdrawnviaintubationtoprepareplateletsuspensionof(0.8-1.0)×1010L-1.②Determinationo

简介：AbstractBackground:Since the diagnostic value of aldosterone to renin ratio (ARR) calculated by plasma renin concentration (PRC) or plasma renin activity (PRA) is still inconclusive, we conducted a meta-analysis by systematically reviewing relevant literature to explore the difference in the diagnostic efficacy of ARR calculated by PRC or PRA, so as to provide guidance for clinical diagnosis.Methods:We searched PubMed, Embase, and Cochrane Library from the establishment of the database to March 2021. We included studies that report the true positive, false positive, true negative, and false negative values for the diagnosis of primary aldosteronism, and we excluded duplicate publications, research without full text, incomplete information, or inability to conduct data extraction, animal experiments, reviews, and systematic reviews. STATA 15.1 was used to analyze the data.Results:The pooled results showed that ARR (plasma aldosterone concentration [PAC]/PRC) had a sensitivity of 0.82 (95% confidence interval [CI]: 0.78-0.86), a specificity of 0.94 (95% CI: 0.92-0.95), a positive-likelihood ratio (LR) of 12.77 (95% CI: 7.04-23.73), a negative LR of 0.11 (95% CI: 0.07-0.17), and symmetric area under the curve (SAUC) of 0.982, respectively. Furthermore, the diagnostic odds ratio (DOR) of ARR (PAC/PRC) was 180.21. Additionally, the pooled results showed that ARR (PAC/PRA) had a sensitivity of 0.91 (95% CI: 0.86-0.95), a specificity of 0.91 (95% CI: 0.90-0.93), a positive LR of 7.30 (95% CI: 2.99-17.99), a negative LR of 0.10 (95% CI: 0.04-0.26), and SAUC of 0.976, respectively. The DOR of ARR (PAC/PRA) was 155.52. Additionally, we conducted a subgroup analysis for the different thresholds (<35 or ≥35) of PAC/PRC. The results showed that the DOR of the cut-off ≥35 groups was higher than the cut-off <35 groups (DOR = 340.15, 95% CI: 38.32-3019.66; DOR= 116.40, 95% CI = 23.28-581.92).Conclusions:The research results suggest that the determination of ARR (PAC/PRC) and ARR (PAC/PRA) was all effective screening tools for PA. The diagnostic accuracy and diagnostic value of ARR (PAC/PRC) are higher than ARR (PAC/PRA). In addition, within a certain range, the higher the threshold, the better the diagnostic value.

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简介：BackgroundTheclinicalcharacteristicsofstrokepatientstreatedwithdoubleanti-platelettherapy(DAPT)afterpercutaneouscoronaryintervention(PCI)isnotclear.MethodsIntotal,2675patientsunderwentPCIandDAPTinGuangdongGeneralHospital,and68outofthemwerehospitalizedduetosuspectedstroke,ofwhom23werediagnosedashavingstroke.Dataofthe23strokepatientswerecollectedandtraditionalriskfactorsassociatedwithstrokewereanalyzedretrospectively.ResultsThemeanageofthesepatientswas75.6±8.7years,and20(87.0%)weremales.Notably,19patientswerecomplicatedwithhypertension,7withdiabetesmellitus,7withprevioushistoryofstroke,nonewithatrialfibrillation(AF)orpatentforamenovale(PFO).Specifically,22patientswerediagnosedwithischemicstroke,and1patientwithhemorrhagicstroke.ConclusionStrokeinpatientstreatedwithDAPTafterPCIwascorrelatedwithadvancedage,gender,hypertension,diabetesmellitus,strokehistory.Longtermelectrocardiography(ECG)maybeneededforthediagnosisofAF,whiletrans-esophagealechocardiography(TEE)maybeneededforPFO.

简介：AbstractImportance:Acute necrotizing encephalopathy (ANE) is a rare disease with high mortality. Plasma exchange (PLEX) has recently been reported to treat ANE of childhood (ANEC), but its efficacy is uncertain.Objective:This study aimed to investigate the effectiveness of PLEX on ANEC.Methods:A retrospective study was conducted in four pediatric intensive care units from December 2014 to December 2020. All patients who were diagnosed with ANEC were included; however, these patients were excluded if their length of stay was less than 24 h. Participants were classified into PLEX and non-PLEX groups.Results:Twenty-nine patients with ANEC were identified, 10 in the PLEX group and 19 in the non-PLEX group. In the PLEX group, C-reactive protein, procalcitonin, alanine aminotransferase, and aspartate aminotransaminase levels were significantly lower after 3 days of treatment than before treatment (13.1 vs. 8.0, P = 0.043; 9.8 vs. 1.5, P = 0.028; 133.4 vs. 31.9, P = 0.028; 282.4 vs. 50.5, P = 0.046, respectively). Nine patients (31.0%, 9/29) died at discharge, and a significantly difference was found between the PLEX group and non-PLEX group [0 vs. 47.4% (9/19), P = 0.011]. The median follow-up period was 27 months, and three patients were lost to follow-up. Thirteen patients (50.0%, 13/26) died at the last follow-up, comprising three (33.3%, 3/9) in the PLEX group and ten (58.8%, 10/17) in the non-PLEX group, but there was no significant difference between the two groups (P = 0.411). Three patients (10.3%, 3/29) fully recovered.Interpretation:PLEX may reduce serum C-reactive protein and procalcitonin levels and improve liver function in the short term. PLEX may improve the prognosis of ANEC, and further studies are needed.

简介：ＴＨＥＣＯＲＲＥＬＡＴＩＯＮＳＴＵＤＹＢＥＴＷＥＥＮＰＬＡＳＭＡＶＩＳＣＯＳＩＴＹＡＮＤＦＩＢＲＩＮＯＧＥＮＣＯＡＧＵＬＡＢＩＬＩＴＹＴＨＥＣＯＲＲＥＬＡＴＩＯＮＳＴＵＤＹＢＥＴＷＥＥＮＰＬＡＳＭＡＶＩＳＣＯＳＩＴＹＡＮＤＦＩＢＲＩＮＯＧＥＮＣＯＡＧ...