简介：Usingultrashort-pulseradarwithcompactvivaldiantennas,thesimulationexperimentofbreastcancerdetectionhasbeenperformedinasyntheticbreastphantom.Twometallicballswith9mmand6mmindiameterareusedastumorsinourexperiment.Theimagereconstructionsofthebreastmodelswithtumorsusingreflectiondatahavebeenpresentedbyaconfocalmicrowaveimagingtechnique.Themethodcanbeusedforapulsecompressiontechniquetoreflectedwavesforclearimageformation.

简介：Breastcanceristheleadingcauseofdeathamongwomenattheagesof20-59years[1].TheincidenceofbreastcancerinAsiaisalsoshowntobeincreasingrecently,especiallyinthemoreaffluentcities,andthepeakincidenceofbreastcancerisattheageof45-50years[2].BreastcancerpreventionhasstartedfromsecondarypreventionstrategyofearlydetectionofthediseasewithdifferentmodalitiesinAsia[3-5]despitetheabsenceofunifiednationwidescreeningprogram.Therearealotof

简介：Objective:Puremucinousbreastcarcinoma(PMBC)isanuncommonhistologicaltypeofbreastcancercharacterizedbyalargeamountofmucinproduction.MicroRNA(miRNA)isalargeclassofsmallnoncodingRNAofabout22ntinvolvedintheregulationofvariousbiologicalprocesses.ThisstudyaimstoidentifythemiRNAexpressionprofileinPMBC.Methods:MiRNAexpressionprofilesin11PMBCswereanalyzedbymiRNA-microarrayandreal-timepolymerasechainreaction(PCR).Thirty-onePMBCsand27invasiveductalcarcinomaofnospecialtypes(IDC-NSTs)wereassessedbyimmunohistochemistryusingantibodiesagainstER,PR-progesteronereceptor,HER2,Ki-67,Bcl-2,p53,PCNA,andCK5and6.Results:WeanalyzedthemiRNAexpressionin11PMBCsandcorrespondingnormaltissuesusingmiRNA-microarrayandreal-timePCR,andfoundthatmiR-143andmiR-224-5pweresignificantlydownregulatedinmucinouscarcinomatissue.ComparedwithIDC-NSTs,PMBCshowedasignificantlyhigherERpositiverate,lowerHER-2positiverate,andlowercellproliferationrates.Conclusions:Toourknowledge,thisisthefirststudytodemonstratethemiRNAexpressionprofileofPMBC,andourfindingsmayleadtofurtherunderstandingofthistypeofbreastcancer.

简介：Objective:Laser-inducedCoulombexplosionofgoldnanoparticlesforbreastcancerhasbeenstudiedbynanophotolysistechnique.Thisstudyaimedtoinvestigatewhetherlaser-inducedbubbleformationduetoCoulombexplosioncanprovideaneffectiveapproachforselectivedamageofbreastcancerwithgoldnanoparticles.Method:Numericalmethodinvolveslaser-inducedCoulombexplosionofgoldnanoparticles.Differentparametersrelatedtonanophotolysissuchaslaserfluence,tumordepth,clusterradius,laserpulseduration,andbubbleformationisstudiednumerically.NumericalsimulationwasperformedusingMatlab.Results:Thegoldnanoparticlesof10,20,30,40,and50nminradiuscouldpenetrateintotumor1.14,1.155,1.189,1.20and1.22cmindepthrespectively.Themaximumpenetrationdepthintumorcouldbeobtainedwithnanoparticlesof50nmradius.Shortlaserpulseof40nswithnanoparticlesof10nmradiuscouldpenetrateintotumor1.14cmindepth.Bubbleswitharadiusof9μmcouldeffectivelykillbreastcancercellswithoutdamaginghealthyones.Thebubbleradiusincreasedfrom4to9μmwithanincreaseinpulsedurationintherangeof10to30ns.Conclusions:Goldnanoparticleswithincreasingradiusandbubbleformationforselectivedamageofbreastcancercellsaresuccessfullyprobed.Thepresentcalculatedresultsarecomparedwithotherexperimentalfindings,andgoodcorrelationisfoundbetweenthepresentworkandpreviousexperimentalvalues.Itwasdemonstratedthatbubbleformationintumormayfurtherincreasetheefficacyofbreastcancertreatment.

简介：Objective:MicroRNA-21(miR-21)hasbeenshowntobeakeyregulatorofcarcinogenesis.TherewerefewreportsaboutthecomparisonofserummiR-21withconventionaltumormarkers.ThisstudyaimedtoexplorethediagnosticvalueofcirculatingmiR-21asatumormarkerinbreastcancer(BC)andcompareitwithCA153andcarcinoembryonicantigen(CEA).Methods:CirculatingmiR-16andmiR-21wereamplifiedandquantitativelydetectedbyreal-timePCRin89BCpatientsand55healthycontrols.ThelevelsofCA153andCEAweremeasuredthroughelectrochemiluminescenceassays.ThenthesensitivityindiagnosisofBCwascomparedamongmiR-21,CA153andCEA.Results:ThelevelofserummiR-21wassignificantlyhigherinBCpatientsthancontrols(P<0.001).ThesensitivityandspecificityofmiR-21were87.6%and87.3%,respectively,whereasthesensitivitiesofCEAandCA153wereonly22.47%and15.73%.Conclusions:ComparedwithCEAandCA153,serummiR-21hasahighersensitivityindiagnosisofBC.AlthoughnotcorrelatedwiththestatusofER,PRandclinicalstages,serummiR-21maybeapotentialdiagnosticindicatorforBC,especiallyfortheearlystage.

简介：Osteopontin(OPN)，多功能的glycoprotein，有在vitro在肿瘤有不同角色的三个抄本。OPN抄本是否在vivo在肿瘤进程有不同函数，是不清楚的。有免疫力的导出房间的OPN能支持肿瘤形成，这被报导了。我们建议导出肿瘤的OPN可以便于的一个假设由影响有免疫力的房间区别和功能的肿瘤免疫者逃跑。在这研究，我们构造了OPN抄本和transfected的lentiviral表示向量他们进MCF-7房间线。有OPN抄本的MCF-7房间transfected被注入裸体老鼠的腋窝，并且肿瘤生长被监视。结果证明所有OPN抄本支持了本地肿瘤形成，而是那在抄本之中没有重要差别。我们也调查了coculturing单核白血球肿瘤房间与肿瘤上层清液在单核白血球区别上表示的OPN的效果。我们与OPN相比发现OPN-cupregulatedCD163层次--andOPN-b；然而，任何一个都没抄本影响HLA医生和CD206层次。所有OPN抄本显著地禁止了TNF-α；并且由单核白血球提高了IL-10生产。而且，我们发现了OPN抄本的overexpression显著地upregulatedTGF-β；1并且由肿瘤房间的MCP-1生产。用抵销抗体和recombinantcytokines，我们发现由肿瘤房间的OPNoverexpressed调整TNF-α的生产；并且由单核白血球的IL-10部分经由MCP-1和TGF-β；1分别地。一起，我们的结果证明OPN抄本没在vivo在乳癌形成有不同角色。我们也证明OPN经由TGF-β调整单核白血球的其他的激活；1并且MCP-1，它可以为肿瘤代表另外的机制免疫者逃跑。