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简介：摘要目的调查研究血清miR-210（非编码单链RNA分子）在肺癌早期诊断中的作用及应用价值。方法随机选取在我院进行肺癌治疗的患者100例，抽取患者血清,根据其诊断指标的不同，将其分为两组,miR-210组50例；RNA组50例。最后对两组患者的诊断标准率进行比较。结果血清miR-210组对肺癌的早期诊断准确率明显高于RNA组，差异显著，统计学上有意义P<0.05，可见，miR-210对肺癌的早期诊断具有高度敏感性和特异性。结论血清miR-210在肺癌早期诊断中具有高度敏感性和特异性,临床上应用血清miR-210检测对肺癌早期诊断具有重要意义。

简介：BackgroundTheremaybedysregulationofcirculatingmicroRNAsinacutemyocardialinfarction(AMI),whichisanaging-relatedprocess.However,thedifferencebetweenyoungandelderlypeopleinexpressionlevelofcirculatingmiR-21inAMIpatientshasnotbeeninvestigated.MethodsThestudyincluded72consecutivepatientswithAMI.ThegroupIconsistedof43patientsagedequaltoorabove65yearsandthegroupIIconsistedof29patientsagedequaltoorbelow45years.Real-timeRT-PCRwasappliedtodetectserummiR-21expressionlevelsatthetimeofmechanicalreperfusionand12h,D1,D3andD7afterPCI,respectively.ResultsTheexpressionlevelofmiR-21inAMIpatientsincreasedmarkedly12hafterPCIandreachedthepeakatD1afterPCIinbothgroups.TherewasnodifferenceofmiR-21expressionbetweenGroupⅠandⅡatthetimeofmechanicalreperfusion(5.12±0.73vs.4.98±0.87)andD7afterPCI(1.28±0.75vs.1.94±0.89),However,groupⅠpatientsexhibitedhighermiR-21expressionlevelthangroupⅡat12h(7.96±0.78vs.4.23±0.77,P<0.05),D1(9.32±0.89vs.6.12±0.92,P<0.05)andD3(4.78±0.91vs.2.97±0.77,P<0.05)afterPCI,respectively.ConclusionOurdatarevealanincreaseofmiR-21inpatientswithAMImaybeamechanismofmyocardialischemiareperfusioninjury.TheexpressionofmiR-21wasrelatedtothedevelopmentandprogressionofAMI,andthereisanage-relatedchangeintheexpressionofmiR-21inacutemyocardialinfarctionpatients.

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简介：Objective:MicroRNA-21(miR-21)hasbeenshowntobeakeyregulatorofcarcinogenesis.TherewerefewreportsaboutthecomparisonofserummiR-21withconventionaltumormarkers.ThisstudyaimedtoexplorethediagnosticvalueofcirculatingmiR-21asatumormarkerinbreastcancer(BC)andcompareitwithCA153andcarcinoembryonicantigen(CEA).Methods:CirculatingmiR-16andmiR-21wereamplifiedandquantitativelydetectedbyreal-timePCRin89BCpatientsand55healthycontrols.ThelevelsofCA153andCEAweremeasuredthroughelectrochemiluminescenceassays.ThenthesensitivityindiagnosisofBCwascomparedamongmiR-21,CA153andCEA.Results:ThelevelofserummiR-21wassignificantlyhigherinBCpatientsthancontrols(P<0.001).ThesensitivityandspecificityofmiR-21were87.6%and87.3%,respectively,whereasthesensitivitiesofCEAandCA153wereonly22.47%and15.73%.Conclusions:ComparedwithCEAandCA153,serummiR-21hasahighersensitivityindiagnosisofBC.AlthoughnotcorrelatedwiththestatusofER,PRandclinicalstages,serummiR-21maybeapotentialdiagnosticindicatorforBC,especiallyfortheearlystage.

简介：目的：研究miR-152分子对NK细胞杀伤JEG-3细胞效应的影响。方法：在滋养细胞肿瘤细胞系JEG-3细胞中分别转染pre-miR-152（实验组）,Cy3标记的pre-miRNA-control（阴性对照）,空白对照（NC）为未转染的JEG-3细胞。转染48h进行NK细胞的细胞毒测定,观察各组NK细胞对JEG-3细胞的杀伤效应。结果：与对照组相比,实验组NK细胞对JEG-3细胞的平均杀伤率显著增高（P〈0.05）,且随着效靶比的增高,杀伤率也增大。空白对照组与阴性对照组之间的NK细胞杀伤能力无显著差异（P〉0.05）。结论：miR-152可以提高NK细胞对滋养细胞的杀伤作用。

简介：目的研究miR-155在结肠癌组织中的表达并探讨其表达与结肠癌临床病理特征的关系。方法收集2011年3月至9月襄阳市中心医院手术切除的原发性结肠癌标本57例，分别提取结肠癌和正常黏膜组织中总RNA并行逆转录反应，应用实时荧光逆转录聚合酶链反应检测肿瘤组织及正常黏膜组织中miR-155的表达水平，探讨其表达与结肠癌临床病理参数间的关系。配对标本采用非参数Wilcoxon秩和检验，两独立样本采用u检验。结果miR-155在结肠癌组织中相对表达水平为0．421（0．016～1．241），显著高于其在正常黏膜组织中表达的0．128（0．000～0．337），两者比较，差异有统计学意义（M=3．783，19〈0．05）；miR-155的表达增高与TNM分期、肿瘤浸润程度、淋巴结转移、肿瘤分化程度明显相关（u=2．364，2．152，2．338，2．214，P〈0．05）。结论miR-155在结肠癌组织中表达增高，miR-155可能作为原癌基因参与结肠癌的发生、发展，与结肠癌的侵袭、转移密切相关。

简介：目的研究miR-146a是否参与新生隐球菌感染免疫应答过程.方法采用RT-PCR检测了6例新生隐球菌性脑膜炎患者和6名健康个体外周血单个核细胞（PBMC）中miR-146a的表达.以热灭活新生隐球菌刺激来自健康个体的PB-MC,并加入Dectin-1抑制剂昆布多糖,采用RT-PCR检测热灭活新生隐球菌和昆布多糖对PBMC中miR-146a表达的影响.结果新生隐球菌性脑膜炎患者PBMC中miR-146a的表达较健康个体明显增高.热灭活新生隐球菌可以上调PBMC中miR-146a的表达,昆布多糖可以削弱其上调miR-146a表达的能力.结论热灭活新生隐球菌可以通过Dectin-1受体上调miR-146a的表达.miR-146a参与了新生隐球菌感染免疫应答过程,值得进一步研究.

简介：Objective:B-celllymphoma2(Bcl-2)isanimportantmemberoftheBcl-2familyofproteinsthatregulatetheinductionofapoptosis.ThisstudyaimstoinvestigatewhetherBcl-2smallinterferingRNA(siRNA)combinedwithmiR-15aoligonucleotides(ODN)couldenhancemethotrexate(MTX)-inducedapoptosisinRajicells.Methods:ChemicallysynthesizedmiR-15aODNandBcl-2siRNAweretransfectedinRajicellsbyusingaHiPerFectTransfectionReagentandthencombinedwithMTX.ExpressionlevelsofBcl-2proteinweredetectedbyWesternblot.CellproliferationwasdeterminedbyCCK8assay.TherateofcellapoptosiswasdeterminedbyAnnexinV/PIdoublestaining.ThemorphologyofapoptoticcellswasobservedbyHoechst-33258staining.Results:AfterthecellsweretransfectedwithmiR-15aODNcombinedwithBcl-2siRNA,Bcl-2proteinlevelswereevidentlydecreased.CCK8assayshowedthatcellproliferationwassignificantlydecreasedandwassignificantlylowerinmiR-15aODNcombinedwithBcl-2siRNAplusMTXgroupthaninmiR-15aODNwithmethotrexategroup,Bcl-2siRNAwithMTXgroup,andsingleMTXgroup(P<0.05).Hoechst33258stainingrevealednumerousapoptoticcells.AnnexinV/PIdoublestainingshowedthattheapoptoticrateswere(13.13±1.60)%,(34.47±2.96)%,(32.87±3.48)%,and(45.47±2.16)%inMTX,Bcl-2siRNAplusMTX,miR-15aODNplusMTX,andmiR-15aODNcombinedwithBcl-2siRNAplusMTXgroups,respectively.Amongthesegroups,theapoptoticrateofmiR-15aODNcombinedwithBcl-2siRNAplusMTXgroupwasthehighest;thisapoptoticratewasalsosignificantlydifferentfromthatofmiR-15aODNorBcl-2siRNAplusMTX(P<0.05).Conclusions:Bcl-2siRNAcombinedwithmiR-15aODNcouldenhanceMTX-inducedapoptosisinRajicells.Bcl-2siRNAandmiR-15acombinedwithMTXmaybeausefulapproachtoimprovethetreatmenteffectsonlymphoma.更多还原

简介：帕金森病（Parkinson＇sdisease，PD）是一种严重危害人类健康的神经退行性疾病，其主要的病理学特征是黑质致密区（SNc）多巴胺能（DA）神经元的进行性丢失和α-突触核蛋白（α—synuclein）异常聚集和沉积形成的Lewy小体。

简介：<正>下肢动脉缺血性疾病是各种原因造成动脉狭窄或闭塞使远端组织循环灌注量减少的一系列疾病。随着人民生活水平日益提高、饮食结构及人口老龄化等因素的改变,慢性下肢缺血性疾病的发病率逐渐增高。如何重建患者肢体循环,降低截肢率,是血管外科医生们亟需解决的问题。慢性下肢缺血性疾病的传统治疗方法包括一般病因治疗、药物治