简介：目的：开发一个新奇方法由DHPLC检测CpGmethylation。方法：在DNA与钠重亚硫酸盐被对待以后，失配修理基因hMLHl倡导者被聚合酶链反应(PCR)放大。DHPLC被用来在他们的部分使中毒的温度分开PCR产品。BstUI消化试金也为比较学习被使用。结果：A294bp乐队被PCR在结肠癌房间线RKO和胃的癌症房间线PACM82的每件DNA样品以后获得。这二个乐队能被DHPLC在53掳C(为RKO的保留时间6.7min对为PACM82的6.2min)完全分开。当PACM82不是methylated时，自从methylation能保护C的变换到T并且在重亚硫酸盐治疗以后使更高的C/G满意，我们断定在RKO房间的hMLHl倡导者是methylated，导致推迟的时间。从BstUI消化试金的与那些一致的这些结果。结论：在hMLHl的CpG岛的Methylation能被DHPLC在重亚硫酸盐修正以后方便地检测。

简介：六高并且从dominantsemi矮子异种(Y98149)导出的矮子near-isogenic线被选择学习高度表示和敏感到gibberellicacid(GA_3)。长度第4-5内部节点，第三，第二，1st内部分别地，从顶的节点和在isogenic附近的六个矮子的圆锥花序长度是在六高的97.2%那些，53.3%，65.1%，61.9%和94.7%显示主导的相形见绌半的基因显著地禁止了内部节点延伸。而且，Y98149(变异的类型)比Y98148(野生型)对GA_3更敏感，并且在植物有更低的GA_3集中，大约78%Y98148。

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简介：Ａ　Ｃｈｒｏｎｏｌｏｇｙ　ｏｆ　ｔｈｅ　Ｒｅｐａｉｒ　ｏｆ　ｔｈｅ　Ｐｏｔａｌａ　ＰａｌａｃｅＡＣｈｒｏｎｏｌｏｇｙｏｆｔｈｅＲｅｐａｉｒｏｆｔｈｅＰｏｔａｌａＰａｌａｃｅ￥ｂｙＷａｎｇＭｉｎｇｘｉｎｇ１９８９Ｏｃｔｏｂｅｒ１－７：ＴｈｅＰｏｔａｌａ...

简介：Immunobiologicalstudyisakeytorevealingtheimportantbasisoffacialnerverepairandregenerationforbothresearchanddevelopmentofclinictreatments.Themicroenvironmentalchangesaroundaninjuriedfacialmotoneuron,i.e.,theaggregationandexpressionofvarioustypesofimmunecellsandmoleculesinadynamicequilibrium,impenetratefromthestarttotheendoftherepairofaninjuredfacialnerve.Theconceptof'immunemicroenvironmentforfacialnerverepairandregeneration',mainlyconcernswiththedynamicexchangebetweenexpressionandregulationnetworksandavariatyofimmunecellsandimmunemoleculesintheprocessoffacialnerverepairandregenerationforthemaintenanceofaimmunemicroenvironmentfavorablefornerverepair.Investigationonmicroglialactivationandrecruitment,Tcellbehavior,cytokinenetworks,andimmunologicalcellularandmolecularsignalingpathwaysinfacialnerverepairandregenerationarethecurrenthotspotsintheresearchonimmunobiologyoffacialnerveinjury.Thecurrentpaperprovidesacomprehensivereviewoftheabovementionedissues.Researchoftheseissueswilleventuallymakeimmunologicalinterventionspracticabletreatmentsforfacialnerveinjuryintheclinic.

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简介：Ａ　Ｃｏｎｇｒａｔｕｌａｔｏｒｙ　Ｎｏｔｅ　ｏｎ　ｔｈｅ　ｓｕｃｃｅｓｓｆｕｌ　Ｒｅｐａｉｒ　ｏｆ　ｔｈｅ　Ｐｏｔａｌａ　ＰａｌａｃｅＡＣｏｎｇｒａｔｕｌａｔｏｒｙＮｏｔｅｏｎｔｈｅｓｕｃｃｅｓｓｆｕｌＲｅｐａｉｒｏｆｔｈｅＰｏｔａｌａＰａｌａｃｅ￥...

简介：ThispaperpresentspracticalresearchonrepairmechanismanditsfourrepairtrajectoriesinFLclassroominteraction.ThisshowsthatitiseffectiveandefficientinassistingFLlearnerstodeveloptheircommunicativecompetenceandunderstandtheprocessoflanguageacquisition.RepairstrategiesthatareofgreatvaluetoFLteachersinFLclassroomteachingarealsoex-pounded.

简介：Recently,manyresearchesontherepairofperipheralnervebytheaidofnerveregenerationconduitmethodwerecarriedoutandgoodprogresseswereattained.Itwasbelievedthattheconduitshouldbemadefrombiodegradablebiomaterial.

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简介：CRTCornercuttingtubeisconsideredasadefinitiverejectandwillbesenttoreclaimatalltimes.Itcausedlotsofmaterialloss.Inthispaper,anoveltechnologyusedtosaveCRTscornercuttingtubeispresentedfirstly.AsetofrepairsystemhasbeensuccessfullydevelopedtorepairCRTscornercuttingtube.Then,theprocesstechnologyforcornercuttingrepairhasbeenexcogitatedbyempiricalstudies.Finally,thecorrelativeperformancesofrepairedtubeareevaluated.Theexperimentalresultsshowthatthequalityofrepairedtubehasnodifferencewiththatofnormalproduct.Therepairyieldhasreachedabove97percent.Thisnewtechnologyhascontributedtoimprovingtheproductionyield,reducingthematerialconsumptionanddecreasingthecostofcolorCRTproduction.

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简介：Wehavereviewedthegenetherapyingastrointestinaldiseases^[1].GastriccanceriscommoninChina^(2-20),anditsearlydiagnosisandtreatmentarestilldifficultuptonow^(13-36).Theex-pressionofanexogenousgeneintroducedbygenetherapyintopa-tientswithgliomascanbemonitorednon-invasivelybypositron-emissiontornography^[4].

简介：Objective:TostudythechangesofthegeneexpressionpatternofspinalcordtissuesintheearlystageafterinjurybyDNAmicroarray(genechip).Methods:ThecontusionmodelofratspinalcordwasestablishedaccordingtoAllen'sfallingstrikemethodandthegeneexpressionpatternsofnormalandinjuredspinalcordtissueswerestudiedbygenechip.Results:Theexpressionof45geneswassignificantlychangedintheearlystageafterspinalcordinjury,inwhich22genesup-regulatedand23genesdown-regulated.Conclusions:Theexpressionofsomegeneschangessignificantlyintheearlystageafterspinalcordinjury,whichindicatesthecomplexityofsecondaryspinalcordinjury.

简介：Thestudyofsmalldrugmoleculesinteractingwithnucleicacidsisanareaofintenseresearchthathasparticularrelevanceinourunderstandingofrelativemechanisminchemotherapeuticapplicationsandtheassociationbetweengenetics(includingsequencevariation)anddrugresponse.Inthiscontribution,wedemonstratehowthesequence-specificbindingofananticancerdrugDacarbazine(DTIC)tosinglebase(A-G)mismatchcouldbesensitivelydetectedbycombiningelectrochemicaldetectionwithbiosensingsurfacebasedongoldnanoparticles.

简介：ThemainpurposeofthispaperistostudyaproblemrelatedtoGolomb'sconjectures,andgiveaninterestingasymptoticformula.

简介：BuddhahallsandsutrahallsofmonasteriesarewheretheTibetanspaysacrificestothedeities.Inaddition,theTibetanscreatenichesforthesamepurposeathome.

简介：Schroedingeroperatorisacentralsubjectinthemathematicalstudyofquantummechanics.ConsidertheSchrSdingeroperatorH=-Δ+VonR,whereΔ=d^2/dx^2andthepotentialfunctionVisrealvalued.InFourieranalysis,itiswell-knownthatasquareintegrablefunctionadmitsanexpansionwithexponentialsaseigenfunctionsof-Δ.AnaturalconjectureisthatanL^2functionadmitsasimilarexpansionintermsof"eigenfunctions"ofH,aperturbationoftheLaplacian(see[7],Ch.XIandthenotes),undercertainconditiononV.

简介：客观；在忍受肝癌症的老鼠调查鼠科的IL-12基因和HSV-TK基因治疗的synergistic反肿瘤效果。方法：老鼠肝癌症MM45T李(H-2d)房间是有包含IL-12基因或HSV-TK基因的retroviral向量的transfected插入修改基因的肝癌症房间，MM45TLi/IL-12和MM45TLi/TK，与IL-12和TK的稳定的表示被获得。Balb/c老鼠与2X105MM45T李房间皮下地被接种。当肿瘤到达了0.5鈥?.0厘米的一种尺寸时，MM45T.Li/TK房间的混合物照耀and60Co的MM45TLi/IL-12房间intratumoraly被注射。Ganciclovir(GCV)是注射ip(40mg.kg?1.d?1)为10天。Intratumoral注射照耀of60Co的MM45TLi/IL-12房间分开在一个星期内被重复两次。有远肿瘤的老鼠根据一样的协议被对待。怒气房间的CTL活动是测量by51Cr版本试金和由染色的immunohistochemical渗入淋巴细胞的肿瘤的显型。结果：在与MM45T对待的老鼠，Li/IL-12或MM45TLi/TK+GCV个别地在肿瘤生长，而是两个都不导致了中等减小能根除肿瘤完全，当时在加GCV与MM45TLi/IL-12和MM45TLi/TK细胞的混合物对待的60%老鼠，完全的肿瘤回归被观察，没有为二个月的肿瘤复发。远肿瘤的生长也在同样对待的老鼠显著地被禁止。大多数收到的老鼠加GCV联合了基因治疗有的丰富的CD4+，CD8+T淋巴细胞渗入。他们的CTL活动比在老鼠显著地高收到的单个基因治疗。有加GCV的IL-12基因和HSV-TK基因的结论联合治疗为老鼠肝癌症是有效的。