简介：ＥＬＥＭＥＮＴＡＬＳＴＵＤＹＯＦＧＥＮＥＴＨＥＲＡＰＹＷＩＴＨＴＨＲＯＭＢＯＰＯＩＥＴＩＮＬｕＣｈｅｎｇｒｏｎｇ陆承荣ＺｈａｏＪｉａｎｚｅｎｇ赵建增ＷａｎｇＸｉａｏｐｉｎｇ王小平Ｌｉｕｌｉ刘丽ＲｅｓｅａｒｃｈＣｅｎｔｅｒｏｆＭｏｌｅｃｕｌａｒＢｉｏｌ...

简介：Duetoconcernsregardingtheoverlappingfiguresinthisreviewthatareidenticaltothosecontainedinareviewarticlethatwehaveco-authoredandpublishedearlier,weretracttheabovepaperwepublishedinCellResearch.Weapologizeforanyconfusionthatmaybecausedbythismatter,althoughwestandbythescientificcontentscontainedintheCellResearchpaper.

简介：ＡｎａｌｙｓｉｓｏｎＤｅｆｉｎｉｔｉｏｎｓＲｅｌａｔｅｄｔｏＰａｒｔｉｃｉｐｔｉｏｎｉｎＳｏｃｉａｌＦｏｒｅｓｔｒｙTXＡｎａｌｙｓｉｓｏｎＤｅｆｉｎｉｔｉｏｎｓＲｅｌａｔｅｄｔｏＰａｒｔｉｃｉｐａｔｉｏｎｉｎＳｏｃｉａｌＦｏｒｅｓｔｒｙＢｙＺｕｏＴｉ...

简介：<正>Canadaisafederalcountrywithafederalgovernment,butaccordingtotheConstitutionAct,1867,"inandforeachProvincetheLegislaturemayexclusivelymakeLawsinrelationtoEducation".Educationisundertheexclusivejurisdictionofprovincialgovernments.Thereisnofederalministryofeducation,butthereisaCouncilofMinistersofEducationtoofferprovincialministriesanddepartmentsofeducationtheopportunitytoworktogether.Ineachprovincethereisaministryorde-

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简介：Inthispaper,theL^2-boundednessofaclassofparametricMarcinkiewiczintegralμa,h^pwithkernelfunctionΩinBq^0.0(s^n-1)forsomeq>1,andtheradialfunctionh(x)∈l∞(L^s)(R+)for1

简介：Directgenetransferintosomatictissueinvivoisadevelopingtechnologywithpotentialapplicationforcancergenetherapy.Retrovirusvector,whichwasaneffectivevehicle,stillhassomedisadvantagesingeneratinghightiterrecombinantvectorsandmanipulatingtomediateinvirogenetransfer.Inthispaper,recombinantvacciniavirusvectorencodinghuman

简介：Objective:Toobserveeffectsofarginineonarterialendotheliuminjuredbyischemia-reperfusion(IR),andexploreitspossiblemechanism.Methods:Fifty-fourratsweredividedinto3groupsandtreatedinrespectiveways:(1)drinkingtapwaterasthecontrol;(2)drinkingtapwatercontaining2.5%L-arginine;(3)drinkingtapwatercontaining2.5L-argininetogetherwithintraperitonealinjectionofN^G-nitro-L-argininemethylester5mg·kg^-1·d^-1.asegmentofthecommoncarotidarterywasoccludedfor1h,andthenreperfused.Samplestakenatdifferentpost-IRtimefromthesegmentwerepreparedfortheultrastructuralandCeH2O2cytochemicalobservation.Thenakedindex(NI)ofinternalelasticlamina(IEL)wasmeasuredforcomparingtheendothelialinjureextentanditsrepairprocess.Results:Lessdamageofendothelialcells(EC),moreplateletsadheringtonakedIELandmoreregeneratingECwereobservedinGroup2.TheNIvaluesofsamplestakenat1,2,3daftertheIRwererespectively0.92±0.08,0.88±0.03and0.41±0.02inGroup1,andreducedto0.52±0.05,0.19±0.08and0.06±0.01inGroup2(P<0.05-0.01).InGroup3,theendotheliumdamagewasnotalleviated,andsoweretheNI.TheCe-H2O2particlesdepositedonthelumensurfaceofendotheliumweremuchlessinGroup2thaninGroups1and3.Conclusions:L-argininepromotestherepairprocessofIR-injuredendotheliumprobablythroughtheremovalofoxygenfreeradicalsbyNO.

简介：Thehomeobox(Hox)genesformanevolutionarilyconservedfamilyencodingtranscriptionfactorsthatplaymajorrolesinsegmentalidentityandorganspecificationacrossspecies.ThecanonicalgroupingofHoxgenespresentintheHOM-CclusterofDrosophilaorrelatedclustersinotherorganismsincludeseight"typical"genes,whicharelocalizedintheorderlabial(lab),proboscipedia(pb),Deformed(Dfd),Sexcombsreduced(Scr),Antennapedia(Antp),Ultrabithorax(Ubx),abdominalA(abdA),andAbdominalB(AbdB).ThemembersofHoxclusterareexpressedinadistinctanteriortoposteriororderintheembryo.AnalysisoftherelatednessofdifferentmembersoftheHoxgeneclustertoeachotherinfourevolutionarilydiverseinsecttaxarevealedthatthelocipb/DfdandAbdB,whicharefarthestapartinlinkage,hadahighdegreeofevolutionaryrelatedness,indicatingthatpb/DfdtypeanteriorgenesandAbdBareclosesttotheancestralanteriorandposteriorHoxgenes,respectively.ThegreaterrelatednessofotherposteriorgenesUbxandabdAtothemoreanteriorgenessuchasAntpandScrsuggestedthattheyarosebygeneduplicationsinthemoreanteriormembersratherthantheposteriorAbdB.

简介：<正>Acutepromyelocyticleukaemia(APL)hasbeenattractingawideinterestfarbeyondhematologicalfieldinthelastdozenyearsduetothepresenceofspecificchromosometranlocationsandclinicalresponsibilitytoall-transretinoicacid(ATRA)bydifferentiationinductionaswellastoarsenictrioxide(ATO).Most(>95%)APLpatientscarryspecificchromosometranslocationt(15;17),whichleadstodiscoveryofPMLgeneonchromosome15.SuchatranslocationcausesthefusionofPMLtoretinoicacidreceptor-alpha

简介：Astructuralgene(750bp),whichcodesforatypeIribosomeinactivatingprotein,trichosanthin,hasbeendesignedaccordingtothecodonusageofhighlyexpressedgeneinE.coliandchemicallysynthesized.Inthesynthesizedgene,twenty-sevenuniquerestrictionsiteswereevenlydispersedwithanaveragedistancebetweentwoadjacentsiteslessthan50bptofacilitateasystematicinvestigationonstructure-functionalrelationshipofthisproteinbysite-directedmuta-genesis.Tosynthesizeit,thewholegenewasdividedintothreelargefragments(EP,PNandNH)whichwereassembledfromseveralchemicalsyntheticoligonucleotidesbyenzymaticmethod.TheassemblyofboththefragmentEPfromsixoligonucleotides(A-F)andthefragmentPNfromfouroligomers(G-J)wascatalyzedbyT4DNAligaseinusingthesinglestrandedDNAmethod[Chen,H.-B.etal,Nucl.AcidsRes.,18,871(1990)].AndfragmentNHwasformedfromthreeduplexesK,LandMbytheclassicaldoublestrandedDNAmethod.Finally,

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简介：Nanogproteinisexpressedintheinteriorcellsofcompactedmorulaeandmaintainedtillepiblastsbutdownregulatedbyimplantationstage.Itisalsoexpressedinembryonicstemcells,embryoniccarcinomacellsandembryonicgermcellsbutdisappearedindifferentiatedEScells.Inthisstudy,wehaveisolated,sequenced,andperformedthefirstcharacterizationoftheNanogpromoter.Thetranscriptionstartsitesweremappedbyprimerextensionanalysis.TwopromoterregionswerefoundupstreamthetranscriptionstartsitesandtheexpressionofmajorNanogpromoter/reportergeneconstructisabolishedindifferentiatedF9ECcellsascomparedtotheundifferentiatedcounterpart.Wealsoshowedthataputativeoctamermotif(ATGCAAAA)isnecessaryforthemajorpromoteractivity.GelshiftandsupershiftassaysshowedthatOct-1,Oct-4andOct-6proteinselectivelybindtotheoctamermotif.

简介：Withthedevelopmentofgenomesequencingformanyorganisms,moreandmorerawsequencesneedtobeannotated.Genepredictionbycomputationalmethodsforfindingthelocationofproteincodingregionsisoneoftheessentialissuesinbioinformatics.Twoclassesofmethodsaregenerallyadopted:similaritybasedsearchesandabinitioprediction.Here,wereviewthedevelopmentofgenepredictionmethods,summarizethemeasuresforevaluatingpredictorquality,highlightopenproblemsinthisarea,anddiscussfutureresearchdirections.

简介：obtainaninitialoverviewofgenediversityandexpressionpatterninporcinethymus,11,712ESTs(ExpressedSequenceTags)from100-day-oldporcinethymus(FTY)weresequencedand7,071cleanedESTswereusedforgeneexpressionanalysis.ClusteredbythePHRAPprogram,959contigsand3,074singletswereobtained.Blastsearchshowedthat806contigsand1,669singlets(totally5,442ESTs)hadhomologuesinGenBankand1,629ESTswerenovel.AccordingtotheGeneOntologyclassification,36.99%ESTswerecatalogedintothegeneexpressiongroup,indicatingthatalthoughthefunctionalgene(18.78%indefensegroup)ofthymusisexpressedinacertaindegree,the100-day-oldporcinethymusstillexistsinadevelopmentalstage.Comparativeanalysisshowedthatthegeneexpressionpatternofthe100-day-oldporcinethymusissimilartothatofthehumaninfantthymus.

简介：Objective:Tostudythevaccinepotencyofgene-modifiedtumorcells.Methods:TheEL-4lymphomawastransducedwithrecombinantretroviruscontainingthemurineGM-CSFgeneorB7-1gene.Theeffectofgenetransductiononantitumorimmunitywasinvestigated.Results:Flowcytometryanalysisshowedthatexpressionoftheirsurfacemarkerbetweenwild-typeEL-4cellsandgenetransducedtumorcellswasthesameexceptforCD80positiveinB7-1genetransducedcells.GM-CSFgeneorB7-1genetransducedEL-4cellsresultedinremarkablelossoftumorigenicityinsyngeneticmice.ThesystemicprotectiveimmunitywasinducedagainstthechallengewithEL-4/wtcells.TherapeuticvaccinewithEL-4/GM-CSForEL/7-1cellscouldretardthegrowthofestablishedearly-stageEL-4/wttumorsignificantly,butnotretardthegrowthoflate-stageEL-4/wttumor.IrradiatedGM-CSFgenetransducedEL-4cellsshowedstrongvaccineeffectagainstEL-4cellchallenge,butirradiatedB7-1genetransducedEL-4cellsshowedweakvaccineeffect.RemarkablecooperativeantitumoreffectagainstEL-4cellchallengewasobservedwhenbothirradiatedEL-4/GM-CSFandEL-4/B7-1wereinoculatedtogether.Conclusion:GM-CSFgeneorB7-1genetransducedcombinationofthetwokindsofvaccinemayhavepotentialapplicationvalueinhumancancertreatment.

简介：Inthispaper,theso-calledlocallikelihoodmethodissuggestedforsolvingthechangepointproblemswhenthedataaredistributedasmultivariatenormal.Thedetectionproceduresproposednotonlyprovidestronglyeonsistentestimatesforthenumberandlocationsofthechangepoints,butalsosimplifysignificantlythecomputation.

简介：有Hopf代数学的至少二种归纳，即pre鈥揌opf代数学和弱Hopf代数学。相应地，我们有二种概括bialgebras，几乎bialgebra和弱bialgebra。让=(，?，我，一，l，r)是一个张肌范畴。由放弃我，l，r并且保留?，一在里面，第一个作者那么得到了鈥揷alledpre鈥搕ensor范畴=(，?，一)并且使用它在Comm描绘几乎bialgebra和pre鈥揌opf代数学。在代数学，32(2)：397鈥?41(2004)。我们在这份报纸的目的是概括张肌范畴=(，?，我，一，l，r)由削弱自然同晶型l，r，即交换自然同晶型ll?1=rr?1个=标志进常规自然转变ll?l=l，rr?有一些另外的条件的r=r并且那么得到鈥揷alled弱张肌范畴以便描绘弱bialgebra和弱Hopf代数学。在这些之间的关系概括了(bialgebras)Hopf代数学和二种类型概括了张肌范畴将被图使用描述。而且，关于弱张肌范畴的一些相关概念和性质将被讨论。关键词弱张肌范畴-弱Hopf代数学-Pre鈥揌opf代数学-Strictization先生(2000)题目分类16W30-18D10-16W35工程在大学里为新世纪优秀才能由节目支持了(号码04鈥?522)，中国(号码10571153)的国家自然科学基础和中国(号码102028)的浙江省的自然科学基础

简介：ＩＮＳＩＴＵＬＡＢＥＬＩＮＧＡＰＯＰＴＯＳＩＳＩＮＢＲＥＡＳＴＣＡＮＣＥＲＡＳＲＥＬＡＴＥＤＴＯＰＲＯＧＮＯＳＩＳＷｕＪｉｏｎｇ吴炅ＳｈａｏＺｈｉｍｉｎ邵志敏ＪｉａｎｇＭｉｎｇ江明ＨａｎＱｉｘｉａ韩企夏ＺｈａｎｇＴｉｎｇｑｉｕ张廷ＳｈｅｎＺｈｅｎｚ...

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