简介:摘要目的为了提高本院检验科尿液分析的准确性,有效性,比较分析GEB-600尿干化学分析仪(简称GEB-600),及IQ200尿沉渣仪(简称IQ200)及手工法显微镜镜检(简称手工法)检测尿液红细胞、白细胞结果。方法随机抽取本院住院患者304份新鲜的尿液标本,分别用GEB-600,IQ200及手工法进行检测,比较分析检测结果。结果GEB-600,IQ200及手工法检测RBC的阳性率分别为21.4%、19.4%、18.8%;WBC的阳性率分别为21.7%、22.7%、22.0%。经χ2检验RBC、WBC的检出率差异无统计学意义(P>0.05)。结论有效的结合尿液干化学分析仪,尿沉渣分析仪及手工显微镜镜检对尿液进行检测,可以快速,准确的为临床提供有效可靠地检验结果。
简介:AIM:ToinvestigatetheroleoftheoverexpressionofB7-H3inapoptosisincolorectalcancercelllinesandtheunderlyingmolecularmechanisms.METHODS:SW620cellsthathighlyoverexpressedB7-H3(SW620-B7-H3-EGFP)andHCT8cellsstablytransfectedwithB7-H3shRNA(HCT8-shB7-H3)werepreviouslyconstructedinourlaboratory.CellstransfectedwithpIRES2-EGFPwereusedasnegativecontrols(SW620-NCandHCT8-NC).Real-timePCRandwesternblottinganalysiswereusedtodetectthemRNAandproteinexpressionsoftheapoptosisregulatorproteinsBcl-2,Bcl-xlandBax.Acellproliferationassaywasusedtoevaluatethesurvivalrateanddrugsensitivityofthecells.Theeffectofdrugresistancewasdetectedbyacellcycleassay.Activecaspase-3westernblottingwasusedtoreflecttheanti-apoptoticabilityofcells.WesternblottingwasalsoperformedtodeterminetheexpressionofproteinsassociatedwiththeJak2-STAT3signalingpathwayandtheapoptosisregulatorproteinsafterthetreatmentwithAG490,aJak2specificinhibitor,inB7-H3overexpressingcells.ThedatawereanalyzedbyGraphPadPrism6usinganon-pairedt-test.RESULTS:WhetherbyoverexpressioninSW620cellsordownregulationinHCT8,B7-H3significantlyaffectedtheexpressionofanti-andpro-apoptoticproteins,atboththetranscriptionalandtranslationallevels,comparedwiththenegativecontrol(P<0.05).AcellproliferationassayrevealedthatB7-H3overexpressionincreasedthedrugresistanceofcellsandresultedinahighersurvivalrate(P<0.05).Inaddition,theresultsofcellcycleandactivecaspase-3westernblottingprovedthatB7-H3overexpressioninhibitedapoptosisincolorectalcancercelllines(P<0.05).B7-H3overexpressionimprovedJak2andSTAT3phosphorylationand,inturn,increasedtheexpressionofthedownstreamanti-apoptoticproteinsB-cellCLL/lymphoma2(Bcl-2)andBcl-xl,basedonwesternblotting(P<0.05).AftertreatingB7-H3overexpressingcellswiththeJak2-specificinhibitorAG490,thephosphorylationofJak2andSTAT3,andtheexp
简介:Er3+/Yb3+codopedzincateBaGd2ZnO5phosphorsweresynthesizedviaatraditionalsolidstatereaction.ThecrystalstructureandphasepuritywerecheckedbymeansofX-raydiffraction(XRD),andtheresultsshowedthatpurephaseBaGd2ZnO5phosphorswithvariousEr3+,Yb3+concentrationswereobtained.TheinfluenceofEr3+andYb3+dopingconcentrationsonthegreenandredupconversionemissionswasstudied.Itwasfoundthatbothgreenandredupconversionemissionsunder980nmexcitationweretwo-photonprocessesindependentfromtherareearthdopingconcentrations.However,theupconversionluminescenceintensitiesgreatlydependedontherareearthdopingconcentration.Furthermore,thepopulationprocessesofupconversionluminescenceandthequenchingmechanismswereanalyzed.Thetemperature-dependentgreenupconversionluminescencewasstudied,andthetemperaturequenchingprocessoftwogreenupconversionemissionswasmodeled.Thethermalquenchingprocessesofthegreenupconversionemissionscouldbewellexplainedbythemodelweproposed.
简介:Zirconiagraphiterefractoryisusedasacore-nozzleinthethin-stripcastingofsteel.Post-mortemanalysisofusedrefractorywasperformedinanefforttoestablishthefailuremechanism.CorrosionbehaviorwasstudiedagainstmoltensteelwithMnO-SiO2basedliquidinclusions.CorrosionofthesematerialsinvolvesdissolutionandoxidationofgraphiteinthematrixfollowedbypenetrationofliquidslagleadingtodegradationofZrO2particles.Thermodynamicequilibriumcalculationswereperformedtostudythiscorrosionmechanism.
简介:尼曼匹克病C型(NPC)是一种常染色体隐性遗传的溶酶体脂质贮积病,主要累及内脏器官和神经系统,自婴幼儿至成人均可发病,儿童期多见。新生儿期持续存在的胆汁淤积性黄疸、脾脏肿大、猝倒发作和垂直性核上性眼肌麻痹为该病的特征性表现,因发病年龄不同,首发的神经系统症状不一致,临床有明显的异质性。NPC基因缺陷导致游离胆固醇转运障碍,在细胞内大量沉积是疾病的始发因素,细胞的自噬功能障碍、钙稳态失平衡、氧化应激等均参与疾病的病理生理过程。通过皮肤成纤维细胞培养发现异常沉积的游离胆固醇或行基因检查发现NPC的致病性突变可确诊该病。美格鲁特是唯一被批准上市的特效药物,早期应用可以改善神经系统症状和延缓疾病的进展。