简介:几种细胞间的粘附分子是,这以前被报导仔细与长期的HBV感染有关。CD2和CD58的建筑群在提高T淋巴细胞的粘附到目标房间,并且支持T淋巴细胞的增生和激活起一个重要作用。在这研究,我们检测了在PBMC的表面上表示的CD2的水平,在PBMC和在有长期的HBV感染的病人的PBMC的CD2积极房间的百分比的CD2mRNA的表达式水平并且把他们与正常控制的表达式水平作比较。我们也与长期的HBV感染并且从正常控制从病人决定了浆液HBVDNA的水平。肝的功能的临床的特征也被测试。结果证明CD2的表示显著地与长期的HBV感染的严厉增加了,它建议CD2可能在长期的HBV感染贡献hepatocyte损坏。
简介:AIMTodeterminetheroleofcorticotropinreleasingfactorreceptor(CRF2)inepithelialpermeabilityandenterocytecelldifferentiation.METHODSForthispurpose,weusedratSpragueDawleyandvariouscoloncarcinomacelllines(SW620,HCT8R,HT-29andCaco-2celllines).ExpressionofCRF2proteinwasanalyzedbyfluorescentimmunolabelinginnormalratcolonandthenbywesternblotindissociatedcolonicepithelialcellsandinthelysatesofcoloncarcinomacelllinesorduringtheearlydifferentiationofHT-29cells(tenfirstdays).ToassesstheimpactofCRF2signalingoncoloniccelldifferentiation,HT-29andCaco-2cellswereexposedtoUrocortin3recombinantproteins(Ucn3,100nmol/L).Insomeexperiments,cellswerepre-exposedtotheastressin2b(A2b)aCRF2antagonistinordertoinhibittheactionofUcn3.Intestinalcelldifferentiationwasfirstanalyzedbyfunctionalassays:thetrans-cellularpermeabilityandthepara-cellularpermeabilityweredeterminedbyDextran-FITCintakeandmeasureofthetransepithelialelectricalresistancerespectively.Morphologicalmodificationsassociatedtoepithelialdysfunctionwereanalyzedbyconfocalmicroscopyafterfluorescentlabelingofactin(phaloidin-TRITC)andintercellularadhesionproteinssuchasE-cadherin,p120ctn,occludinandZO-1.Theestablishmentofmatureadherensjunctions(AJ)wasmonitoredbyfollowingthedistributionofAJproteinsinlipidraftfractions,afterseparationofcelllysatesonsucrosegradients.Finally,themRNAandtheproteinexpressionlevelsofcharacteristicmarkersofintestinalepithelialcell(IEC)differentiationsuchasthetranscriptionalfactorkrüppel-likefactor4(KLF4)orthedipeptidylpeptidaseIV(DPPIV)wereperformedbyRT-PCRandwesternblotrespectively.ThespecificactivitiesofDPPIVandalkalinephosphatase(AP)enzymesweredeterminedbyacolorimetricmethod.RESULTSCRF2proteinispreferentiallyexpressedinundifferentiatedepithelialcellsfromthecryptsofcolonandinhumancoloncarcinoma
简介:摘要:患儿,魏某,7岁,李某,7岁,均因“反复周期性发热”入院,发病时体温在38.0°~40.5°之间,发热呈明显周期性,魏某3周发作一次,李某4周发作一次,同时伴咽部不适、颈部淋巴结肿大,有时有口腔溃疡,无其余明显不适,给予抗生素后,上述症状无缓解,给予激素(地塞米松)治疗后症状可迅速缓解。在外院行淋巴结活检、风湿免疫指标,血常规,肝肾功等检查,均未见明显异常。二人分别就诊于山东第一医科大学附属医院,排除伴有其他周期性发热的自身免疫病、感染性疾病及恶性肿瘤疾病,诊断为“周期性发热 - 阿弗他口炎 - 咽炎 – 颈淋巴结炎(PFAPA)”,随后入院行“扁桃体切除术”,术后恢复正常,出院后行随访后发现两位患儿的周期性发热消失。