简介：

简介：客观；为了评估临床的申请并且在皮肤上讨论反向的背面的掌部的拍动和它的复合拍动的起作用的指示，hand.Methods背叛：从1990～2003，我们使用了反向的背面的掌部的拍动和它的复合拍动在122种情况中修理手指的软织物缺点，它与腱接枝，神经接枝或骨头接枝包括了反向的掌部的拍动的90个盒子和它的复合拍动的32个盒子。基于后续观察，我们分析了在比较tocontralateral的掌部的拍动和它的复合拍动，手术后的轮廓，拍动颜色和质地回顾地摸的颠倒的指示。结果：在122个盒子的系列，熬过的拍动和施主地点缺点直接被关上。后续时期从1-12年。拍动和它的复合拍动的Thepostoperative轮廓，颜色和质地类似于正常手指的那些，尽管线性疤留下了。根据感觉恢复的标准(英国医药研究委员会，BMRC)，拍动的感觉功能在操作以后恢复了S31年。在有拍动与腱接枝对待的腱缺点的10种情况中，手指的屈曲扩展的功能用全部的活跃运动的测量的方法与相反地侧面的手指相比恢复了50%-75%。在有不属于工会的phalangeal或拍动与骨头接枝对待的骨头缺点的7种情况中，联合为3months.Conclusions发生在操作以后：到有骨头或腱暴露的手指上的软织物缺点，反向的掌部的拍动和它的复合拍动是为修理的一种更好的选择。修理的范围直到手指的远侧的interphalangeal关节。第二根背面的掌部的动脉象脉管的小花梗的选择更一致、更大，与另外的背面的掌部的动脉比较。手术后的拍动颜色和质地类似于正常手指。

简介：

简介：位和手掌的degloving损害仍然是坚持的挑战。我们使用了在前面偏一边的大腿拍动与这个索引，中间和戒指手指的degloving损害对待一个18岁的、右手的男工人。拍动被指定包支撑degloving损害的三根手指的全部圆周并且形成露指手套手。三根手指的远侧的方阵的全部的长度被保留几乎完全。施主缺点被裂口厚度皮肤接枝盖住。在第一操作以后的三个月，X射线学揭示了终端phalanxexr

简介：

简介：讨论联合外部fixator和皮肤拍动调换的效果在上的目的暴露并且不属于工会的骨头。开的骨折在1998年3月的经期期间在我们的医院里对待到2008年6月的感染的不属于工会、暴露的骨头列在后面的12个盒子的数据被分析的方法。有10个男病人，2个女病人，其变老在19-52年之间并且平均28年。有10胫骨的破裂和2大腿骨的破裂。疾病的功课与19个月的吝啬的时期持续了12-39月。所有盒子被联合外部fixator和皮肤拍动调换对待。结果主要愈合在10种情况中被完成并且推迟在胫骨由于软织物缺点和因此本地的拍动调换在被暴露的2种情况中愈合被执行。所有12个盒子与8个月的平均时间在操作以后在6-12月以内有多骨的联合。他们被跟随在上面为1-3，年和所有破裂愈合了在上面与好功能和没有感染复发。联合外部fixator和皮肤拍动调换治疗在对待上看了最佳的效果的结论感染了，暴露并且不属于工会的骨头

简介：Objective:Toobservetherevascularizationandtheopportunityofcross-fingerflap.Methods:Ananimalmodelwasdevelopedtopermitdailymonitoringofneovascularizationoftheflapwithautoradiography,tissuetransparenttechnique,grossobservationandhistologicalexamination.Results:Therevascularizationoftheflapwaschieflyraisedfromthesurroundingtissues.Thepediclesof334cross-fingerflapsofthepatientsweresuccessfullydividedfrom1to5dafteroperations,averagely3.3d.Allofthecasesshowedsatisfatoryresultsaccordingtoafollow-upsurveyof3to72mon.theresultsfurtherprovedthattherevascularizationofthecross-fingerflapshadaccomplishedwithin3d.Conclusions:Itsuggeststhatthedivisionofacrossfingerflapcanbecarriedoutbetweenthe3rdand5thdayafteroperationwithahighdegreeofsafety.

简介：客观：当在骨头织物工程(TE)和有钛蜂窝胃的公山羊的mandible的部分缺点的修理的脚手架增强了，调查使用自然poritos的可行性。方法：有在大约50%-65%的尺寸和孔的190-230μm的一个毛孔的自然poritos被塑造进小粒的形状5公里x5公里x在尺寸的5尼姑。扩展的自体同源的公山羊的髓间充质的干细胞被recombinant导致人的形态基因的protein-2(rhBMP2)到改进osteoblastic显型。然后，髓导出造骨细胞在4x10~7/ml的密度被播种进poritos并且在培植以前在vitro孵化了48个小时。然后，osteoblasticcells/poritos建筑群被植入进mandible的缺点，缺点被钛蜂窝胃增强。poritos的培植独自扮演了著名计算机生产厂商他控制。骨头新生被估计4，8，在用roentgenographicanalysis和组织学的观察的培植以后的16个星期在16个星期以后被做。结果：新骨头能是observedhistologically在表面上并且在在在cell-seedinggroup的所有标本的自然珊瑚的毛孔，而在控制组没有在构造的中心的成骨过程的证据。结果证明新骨头接枝16个星期成功地在培植以后被恢复。结论：当与髓移植的支架材料由TE方法导出造骨细胞，这研究建议使用多孔的珊瑚的可行性。借助于钛蜂窝胃加强，mandible的缺点能成功地被恢复。它显示出在诊所为骨头缺点的重建使用这个方法的潜力。

简介：

简介：客观：在旧王牌上与骨膜调查免费的肠骨的冠接枝的治疗学的有效性平坦缺点。方法：从1996年2月到2005年6月，9patients与骨膜与免费的肠骨的冠接枝被对待重建旧acetabulardefects。有7男性和2女性，平均年龄是41。3年。acetabulardefects被交通事故在3种情况中在6种情况和秋天损害中引起。处理的从损害的时间是4-13月并且平均8个月。Intraoperatively，我们第一移开以后的墙的theacetabular破裂碎片。大腿骨的头当时是reducted。骨头接枝与骨膜从肠骨的冠被收获，它与骨钳被雕刻遵循缺点。凹面(肠骨的窝)接枝的方面被放向大腿骨的头。Thegraft被二～三个腿螺丝钉安全地修理。结果：手术后的症候群没在任何盒子中被发现。哈里斯“20个系统证明分数从32涨了。3pointspreoperatively手术后地指向81。新潮的功能在3种情况中作为优秀被评估，在在2种情况中的4种情况和交易会中好。结论：尽管这个过程不能确切复制新潮的关节的解剖，它启用恢复以后的稳定性，为新潮的关节的providebone股票并且阻止大腿骨的头的脱臼。

简介：

简介：

简介：

简介：Objective:Tostudytheeffectofmildhypothermiaonglucosemetabolismandglycerolofbraintissueinpatientswithseveretraumaticbraininjury(STBI)usingclinicalmicrodialysis.Methods:Thirty-onepatientswithSTBI(GCS≤8)wererandomlydividedintohypothermicgroup(GroupA)andcontrolgroup(GroupB).Microdialysiscatheterswereinsertedintothecerebralcortexofperilesionalandnormalbraintissue.AllsampleswereanalyzedusingCMAmicrodialysisanalyzer.Results:Incomparisonwiththecontrolgroup,lactate/glucoseratio(L/G),lactate/pyruvateratio(L/P)andglycerol(Gly)inperilensionaltissueweresignificantlydecreased;L/Pinnormalbraintissuewassignificantlydecreased.Incontrolgroup,L/G,L/PandGlyinperilensionaltissuewerehigherthanthatinnormalbraintissue.Inthehypothermicgroup.L/Pinperilensionaltissuewashigherthanthatinrelativenormalbrain.Conclusions:MildhypothermiaprotectsbraintissuesbydecreasingL/G,L/PandGlyinperilensionaltissueandL/Pin'normalbrain'tissues.Theenergycrisisandmembranephospholipiddegradationinperilensionaitissueareeasiertohappenaftertraumaticbraininjury,andmildhypothermiaprotectsbrainbetterinperilensionaltissuethaninnormalbraintissue.

简介：Objective:ToprovideahighlyefficientadenoviralvectorAd-CMV-hTGFβ1forthestudyofgenetherapyforreversionoftheintervertebraldiscdegeneration.Methods:Anewlydevelopedrecombinantadenoviralvectorconstructionsystemwasusedinthestudy.ThecDNAofhTGFβ1wasfirstsubclonedintoashuttleplasmidpShuttle-CMV.TheresultantplasmidwaslinearizedbydigestingwithrestrictionendonucleasePmeI,andsubsequentlytransformedintoE.coll.BJ5183cellswithanadenoviralbackboneplasmidpAdEasy-1.Recombinantswereselectedbykanamycinresistanceandconfirmedbyrestrictionendonucleaseanalysis.Finally,therecombinantplasmidlinearizedbyPmeIwastransfectedinto293cells.Recombinantadenovirusesweregeneratedwithin2weeks.Results:TherecombinantadenoviralplasmidswerecutbyBamHIandPacIrespectively,andthediagnosticfragmentsappearedin0.8%agaroseelectrophoresis.Theinfected293cellsshowedevidentcytopathlceffect(CPE).TheproductionsofPCRconfirmedthepresenceofrecombinantadenovirus.TheexpressionofhTGFβ1wasverifiedbyimmunohistochemicalstaining.Conclusions:ThesuccessfulgenerationoftheadenoviralvectorAd-CMV-hTGFβ1andtheconfirmationoftheinterestgeneexpressionmakeitpossiblefortheexperimentalstudyofthereversionoftheintervertebraldiscdegenerationbygenetherapy.

简介：Objective:Todetectsuperoxideanion(O-2)signalsintheheart,liver,lungandkidneytissuesafterburns.Methods:Twenty-fourmaleratswererandomizedinto4groups.Theratsofexperimentalgroupswereimmersedin100℃waterfor15secondsand25%third-degreeburnwascreated.Thoracotomyorlaparotomywasperformedat5,10,15,20and30minutesafterburn,andspecimensoftheheart,lung,liverkidneywereobtainedforburnedrats.Thespecimenswerethenpreservedinliquidnitrogenforcryo-preservationanddetectedimmediatelyusingEPR.Results:ThesignalsofsuperoxideOradicalappearedintheheart,lung,liverandkidneyspecimens10-15minutesafterburn.Conclusions:Thereisadirectevidenceofoxygenfreeradicals(OFRs)injurytovisceraofburnedratsbetween10-15minutesafterburn.

简介：摘要目的探讨Tissue-Tek®全自动染色封片工作站在常规病理染色中的应用及与手工染色封片的区别。方法通过Tissue-Tek®全自动染色封片工作站，设定程序进行HE染色封片处理，并与手工染色封片结果进行对比分析。结果与手工染色封片相比，Tissue-Tek®全自动染色封片工作站制作的石蜡切片特点总结为细胞质、细胞核结构清晰可见并对比明显，染色均匀，色彩鲜明，无气泡、溢胶现象。且更换不同操作者上机操作，不同批次间组织切片染色质量均衡。结论应用Tissue-Tek®全自动染色封片工作站，能够缩短染色时间，确保染色目标及时实现，从整体上优化染色质量。符合相关标准以及要求，还能降低人为操作失误率。

简介：Objective:Tostudytheeffectofhyperventilationonbraintissueoxygenpressure(PtiO2),braintissuecarbondioxidepressure(PtiCO2),pHvalueandintracranialpressure(ICP)duringintracranialhypertensioninpigs.Methods:Autologousarterialblood(5.5ml±0．5ml)wasinjectedintotheleftfrontallobebymicropumptoestablishthemodelofintracerebralhematomainpigs.Afterbloodinjection,theanimalswerehyperventilatedfor15minutestodecreasethepressureofcarbondioxideinarterialblood(PaCO2)to27.35mmHg±11.97mmHg(1mmHg=0.133kPa).Themeanarterialpressure(MAP),intracranialpressure(ICP),cerebralperfusionpressure(CPP),PtiO2,PtiCO2,pHvalueand[HCO3^-]werecontinuouslymonitoredandthebloodgaswasanalyzed.Results:Afterhyperventilation,theICPsignificantlydecreased(P<0.01),theCPPsignificantlyincreased(P<0.05),whilethePtiO2greatlydecreasedtotheischemiclevel(8.20mmHg±2.50mmHg)(P<0.01),thePtiCO2decreased(P<0.01)andthepHvalueincreased(P<0.01).Atthesametime,bloodgasanalysisshowedthatthePaCO2greatlydecreasedandthepHvalueincreased.Conclusions:HyperventilationcandecreasetheICPandthePtiO2significantly.Therefore,hyperventilationshouldbeavoidedearlyafterbraininjury.ThePtiO2monitoringwillbehelpfulfordetectingcerebralischemiaearly.

简介：Objective:Tostudythechangesofpartialpressureofbraintissueoxygen(PbtO2)andbraintemperatureinacutephaseofsevereheadinjuryduringmildhypothermiatherapyandtheclinicalsignificance.Methods:Onehundredandsixteenpatientswithsevereheadinjurywereselectedanddividedintoamildhypothermiagroup(n=58),andacontrolgroup(n=58)accordingtooddandevennumbersofhospitalization.WhilemildhypothermiatherapywasperformedPbtO2andbraintemperatureweremonitoredfor1-7days(mean=86hours),simultaneously,theintracranialpressure,rectumtemperature,cerebralperfusionpressure,PaO2andPaCO2werealsomonitored.Thepatientswerefollowedupfor6monthsandtheprognosiswasevaluatedwithGOS(Glasgowoutcomescale).Results:ThemeanvalueofPbtO2within24hourmonitoringinthe116patientswas13.7mmHg±4.94mmHg,lowerthanthenormalvalue(16mmHg±40mmHg)ThetimeofPbtO2recoveringtothenormalvalueinthemildhypothermiagroupwasshortenedby10±4.15hourscomparedwiththecontrolgroup(P<0.05).Thesurvivalrateofthemildhypothermiagroupwas60.43%,higherthanthatofthecontrolgroup(46.55%).Aftertherecoveryofthebraintemperature,PbtO2increasedwiththeriseofthebraintemperature.Conclusions:Mildhypothermiacanimprovethesurvivalrateofsevereheadinjury.ThetechniqueofmonitoringPbtO2andthebraintemperatureissafeandreliable,andhasimportantclinicalsignificanceinjudgingdiseaseconditionandinstructingclinicaltherapy.

简介：Objective:Toobservetheinfluenceofintra-articularinjectionofsodiumhyaluronate(HA)onthemRNAexpressionsofmatrixmetalloproteinase-1,-3(MMP-1,-3)andtissueinhibitorofmetalloproteinase-1(TIMP-1)incartilageandsynoviumoftraumaticosteoarthritis(OA).Methods:Sixteenwhiterabbitsunderwentunilateralanteriorcruciateligamenttransection(ACLT)weredividedinto2groupsrandomly5weeksaftertransection.Theexperimentalgrouprabbitsreceived0.3mlof1%HAbyintra-articularinjectiononceaweek.Animalsinthecontrolgroupweretreatedunderthesameconditionsusingphysiologicalsaline.Tenweeksfollowingsurgery,cartilageandsynoviumwereharvested.ThemRNAexpressionsofMMP-1,MMP-3andTIMP-1wereanalyzedusingreversetranscription-polymerasechainreaction(RT-PCR).Results:Insynovium,themRNAexpressionofMMP-3wassuppressedintheHAinjectiongroup.HAtreatmenthadnoeffectontheMMP-3expressionincartilage.NosignificantdifferenceofMMP-1andTIMP-1expressionsincartilageandsynoviumwasfoundbetweentheHAinjectiongroupandthecontrolgroup.Conclusions:OneofthemechanismsofthetherapeuticeffectofHAmaybetheinhibitionofexpressionofMMP-3insynoviumduringearlystageoftraumaticOA.