简介：Objective:Toinvestigatethecorrelationbetweentraumaandpulmonarythromboembolism.Methods:Comminutedfracturesandextensivesoft-tissuecontusionatbothhindlimbsweremadebyafallingweightfromaheightin16rabbits.Lungperfusionscanningwasperformedtoobtaintheradioactivitycountsbeforetrauma,at1h,48hand96haftertrauma.Allthedataweredividedinto4groupsbasedontheabove4timepoints.Therabbitsweresacrificedwhenpositivefindingsonthepulmonaryperfnsionscanningappeared.Theirlungswereharvestedtobeparaffin-embeddedandstainedwithhematoxylin-erosinmethodforhistologicalexaminationofthromboembolism.TherandomizedblockdesignANOVAandthemethodofleastsignificantdifference(LSD)wereusedforstatisticalanalysisoftheradioactivitycounts.Results:Thehistologicalfindingsshowedthatpulmonaryembolismdevelopedin6ofthe16rabbits(37.5%).Fiveofthe6pulmonaryembolismrabbitspresentedneitherclinicalsymptomsnorpositivepulmonaryembolismmanifestationsinthelungperfusionscanning.Asignificantdifferencewasfoundinlungperfusionradioactivitybetweenthepre-traumatic,post-traumatic1hgroupsandpost-traumatic48hand96hgroups(P<0.05).Conclusions:Fracturesofthehindlimbsaccompaniedwithextensivesoft-tissuecontusionmaycausepulmonarymicro-embolismthatisnotsensitivetolungperfusionscanningandtendstohavenoclinicalsymptoms.Pulmonaryembolismdevelopmentmaytakemorethantwodaysaftertrauma.

简介：Objective:Tostudythecorrelationbetweenbrainedema,elevatedintracranialpressure(ICP)andcellapoptosisintraumaticbraininjury(TBI).Methods:Inthisstudy,totally42rabbitsin7groupswerestudied.Sixoftheanimalswereidentifiedasacontrolgroup,andtheremaining36animalswereequallydividedinto6TBIgroups.TBImodelswereproducedbythemodifiedmethodofFeeney.Aftertheimpact,ICPofeachsubjectwasrecordedcontinuouslybyanICPmonitoruntiltheanimalwassacrificedatscheduledtime.Theapoptoticbraincellsweredetectedbyanterminaldeoxynucleotide-transferase-mediateddUTP-digoxigeninnickendlabeling(TUNEL)assay.Cerebralwatercontent(CWC)wasmeasuredwithadryingmethodandcalculatedaccordingtotheElliottformula.Then,ananalysiswasconductedtodeterminethecorrelationbetweenthecountofapoptoticcellsandtheclinicalpathologicalchangesofthebrain.Results:Apoptoticcellcountbegantoincrease2haftertheimpact,andreacheditsmaximumabout3daysaftertheimpact.ThepeakvalueofCWCandICPappeared1dayand3daysaftertheimpact,respectively.ApoptoticcellcounthadapositivecorrelationwithCWCandICP.Conclusions:InTBI,occurrenceofbrainedemaandICPincreasemightleadtoapoptosisofbraincells.Anytherapywhichcanrelievebrainedemaand/ordecreaseICPwouldbeabletoreduceneuronapoptosis,therebytoattenuatethesecondarybraindamage.

简介：Objective:Toobservetherevascularizationandtheopportunityofcross-fingerflap.Methods:Ananimalmodelwasdevelopedtopermitdailymonitoringofneovascularizationoftheflapwithautoradiography,tissuetransparenttechnique,grossobservationandhistologicalexamination.Results:Therevascularizationoftheflapwaschieflyraisedfromthesurroundingtissues.Thepediclesof334cross-fingerflapsofthepatientsweresuccessfullydividedfrom1to5dafteroperations,averagely3.3d.Allofthecasesshowedsatisfatoryresultsaccordingtoafollow-upsurveyof3to72mon.theresultsfurtherprovedthattherevascularizationofthecross-fingerflapshadaccomplishedwithin3d.Conclusions:Itsuggeststhatthedivisionofacrossfingerflapcanbecarriedoutbetweenthe3rdand5thdayafteroperationwithahighdegreeofsafety.

简介：GenemodificationoftumorcellstoexpressavarietyofcytokinessuchasIL-2ortheco-stimulatorymoleculeB7.1ledtoincreasedimmunogenicityandreducedtumorigenicityoftumorshasseveralmodelsandthisprocessinvolvesTcells.WehavepreviouslyreporteddecreasedtumorigenicityofthemurineplasmacytomaJ558L(MHCclassⅠ~+andclassⅡ~-)expressingIL-2orB7.l.Whensystemicimmunitywasanalyzed,