简介：AIMTo估计角膜的矩阵作为脚手架过去常重建的非细胞组成的驼鸟张力亢进的盐溶液与一个消化方法相结合的损坏cornea.METHODSA习惯于decellularize驼鸟角膜。非细胞组成的角膜的矩阵的微观结构被传播电子显微镜学(TEM)和hematoxylin和曙红观察(H&；E)染色。机械性质被一个电流计和一台紧张机器检测。非细胞组成的角膜的矩阵也被移植进一个兔子角膜和cytokeratin3被用来检查有免疫力的phenotype.RESULTSThe微观结构和驼鸟角膜的机械性质很好在decellularization过程以后被保存。在vitro，甲基thiazolyltetrazolium结果表明非细胞组成的驼鸟角膜(AOC)的摘录没在增长上有禁止的效果角膜上皮或endothelial房间或在keratocytes上。兔子当角膜的混浊和接枝溶解发生在非细胞组成的猪的角膜(APC)时，薄片状的keratoplasty证明移植AOC进主人角膜透明、完全合并移植。重建的角膜的显型类似于有3首先在表面的上皮的房间layer.CONCLUSIONWe使用了的cytokeratin的高表情的一个正常兔子角膜重建的脚手架损坏了角膜的AOC。与猪的角膜相比，驼鸟角膜的解剖结构人的角膜接近那些。根据原则，那结构决定功能，薄片状的keratoplasty也证实了的异种皮移植AOC移植与APC接枝的相比产生了优异结果。

简介：AIM:Toconstructfunctionalhumanfull-thicknesscornealreplacements.METHODS:Acellularporcinecornealmatrix(APCM)wasdevelopedfromporcinecorneabydecellulariztion.Thebiomechanicalpropertiesofanterior-APCM(AAPCM)andposterior-APCM(PAPCM)werecheckedusinguniaxialtensiletesting.Humancornealcellswereobtainedbycellculture.Suspendingringwasdesignedbydeformationofanacupunctureneedle.MTTcytotoxicityassaywasusedtocheckthecytotoxicityofsuspendingringsoakingsolutions.Anewthree-dimensionalorganculturesystemwasestablishedbycombinationofsuspendingring,48-wellplateandmediumtogether.Ahumanfull-thicknesscornealsubstitutewasconstructedfromhumancornealcellswithAAPCMinanorgancoculturesystem.Biochemicalmarkerexpressionoftheconstructwasmeasuredbyimmunofluorescentstainingandmorphologicalstructureswereobservedusingscanningelectronmicroscopy.Pumpfunctionandbiophysicalpropertieswereexaminedbypenetratingkeratoplastyandfollow-upclinicalobservations.RESULTS:TherewerenocellsintheAAPCMorPAPCM,whereascollagenfibers,Bowman’smembrane,andDescemet’smembranewereretained.ThebiomechanicalpropertyofAAPCMwasbetterthanPAPCM.HumancornealcellsgrewbetterontheAAPCMthanonthePAPCM.Therewasnocytotoxicityforthesuspendingringsoakingsolutions.Fortheconstructedfull-depthhumancornealreplacementskeratocytesscattereduniformlythroughouttheAAPCMandexpressedvimentin.TheepitheliallayerwaslocatedonthesurfaceofBowman’smembraneandcomposedofthreeorfourlayersofepithelialcellsexpressingcytokeratin3.OnelayerofendothelialcellscoveredthestromalsurfaceofAAPCM,expressedNa+/K+ATPaseandformedtheendotheliallayer.Theconstructwassimilartonormalhumancorneas,withmanymicrovilliontheepithelialcellsurface,stromalcellswithalongshuttleshape,andzonulaoccludensontheinterfaceofendothelialcells.Theconstructwithstoodsurgicalprocedu