简介:Objective:Puremucinousbreastcarcinoma(PMBC)isanuncommonhistologicaltypeofbreastcancercharacterizedbyalargeamountofmucinproduction.MicroRNA(miRNA)isalargeclassofsmallnoncodingRNAofabout22ntinvolvedintheregulationofvariousbiologicalprocesses.ThisstudyaimstoidentifythemiRNAexpressionprofileinPMBC.Methods:MiRNAexpressionprofilesin11PMBCswereanalyzedbymiRNA-microarrayandreal-timepolymerasechainreaction(PCR).Thirty-onePMBCsand27invasiveductalcarcinomaofnospecialtypes(IDC-NSTs)wereassessedbyimmunohistochemistryusingantibodiesagainstER,PR-progesteronereceptor,HER2,Ki-67,Bcl-2,p53,PCNA,andCK5and6.Results:WeanalyzedthemiRNAexpressionin11PMBCsandcorrespondingnormaltissuesusingmiRNA-microarrayandreal-timePCR,andfoundthatmiR-143andmiR-224-5pweresignificantlydownregulatedinmucinouscarcinomatissue.ComparedwithIDC-NSTs,PMBCshowedasignificantlyhigherERpositiverate,lowerHER-2positiverate,andlowercellproliferationrates.Conclusions:Toourknowledge,thisisthefirststudytodemonstratethemiRNAexpressionprofileofPMBC,andourfindingsmayleadtofurtherunderstandingofthistypeofbreastcancer.
简介:ObjectiveTocharacterizemicroRNA(miRNA)expressionprofileinmicrodissectedauditoryepitheliafromtheCorti'sOrganinnewbornandadultrats.MethodsTheTaqManMicroRNAArrayswereusedtoidentifyexpressionofmicroRNAinthenewbornandadultgroups.GOanalysiswasappliedtoanalyzethemainfunctionofthedifferentialexpressiongenesaccordingtotheGeneOntologywhichisthekeyfunctionalclassificationofNCBI.Similarly,PathwayanalysiswasusedtofindoutthesignificantpathwayofthedifferentialgenesaccordingtoKEGG,BiocartaandReatome.ResultsIncreasedexpressionwasseenin16miRNAsinmatureratcomparedtonewbornrats,withincreasedfoldingrangingfrom17to600folds.Expressionlevelsin2miRNAswerereducedinmaturerats,namelyrno-miR-29candrno-miR-29a.Thehigh-enrichmentGOstargetedbyover-expressedmiRNAswerenegativeregulationofepithelialcelldifferentiation,common-partnerSMADproteinphosphorylation,mesenchymal-epithelialcellsignaling,regulationoftransforminggrowthfactorbeta2production,etc.FunctionalanalysisofmiRNAsbyKEGGrevealedthat19signaltransductionpathwayswereupregulatedand14weredownregulated.ConclusionsThedifferenceinmiRNAexpressionpatternsintheorganofCortibetweenneonatalandadultratsmaybecloselyrelatedtomaturationoftheorganofCortiandlossofproliferativecapacityofinnerearhaircells,andTGFβsignalingmayplayanimportantroleinhaircellsregeneration.
简介:目的通过对结直肠癌患者血清进行实时定量PCR检测,筛选出结直肠癌转移相关的miRNA。方法通过文献检索,筛选出11个结直肠癌转移相关的miRNA(miRs-31,335,206,141,126,200b,200c,21,Let7a,Let7b和Let7c),收集2007年7月至2013年4月北京友谊医院收治的术前留置血清的结直肠癌转移(IV期)病例共计108例,其中结直肠肝转移患者72例,其他脏器转移患者36例;并纳入2008年1月至2010年6月术前留置血清的局灶性结直肠癌(L-CRC)(I-III期)病例共计116例作为对照组进行研究。通过对两组患者血清样本进行实时定量PCR检测结果找出结直肠癌转移相关miRNA。结果筛选出11个转移相关的miRNA中的7个miRNA(miRs-31、141、126、21、Let7a、Let7b和Let7c)可以从血清中被检测到。与局灶性结直肠癌患者相比,miR-141,miR-126,Let-7a和miR-21在转移性结直肠癌患者的血清中表达具有显著性差异(P〈0.0001,P〈0.0001,P=0.0120和P〈0.0001),可以作为结直肠癌转移相关miRNA。结论7个转移相关MiRNAs:miRs-31、141、126、21、Let7a、Let7b和Let7c可以从结直肠癌患者血清中被检测到;miR-141,miR-126,Let-7a和miR-21可以作为结直肠癌转移相关miRNA。
简介:MicroRNAs(miRNAs)aredynamicallyregulatedduringneurodevelopment,yetfewreportshaveexaminedtheirroleinspinabifida.Inthisstudy,weusedanestablishedfetalratmodelofspinabifidainducedbyintragastricallyadministeringoliveoil-containingall-transretinoicacidtodamsonday10ofpregnancy.Damsthatreceivedintragastricadministrationofall-transretinoicacid-freeoliveoilservedascontrols.ThemiRNAexpressionprofileintheamnioticfluidofratsat20daysofpregnancywasanalyzedusinganmiRNAmicroarrayassay.Comparedwiththatincontrolfetuses,theexpressionofmiRNA-9,miRNA-124a,andmiRNA-138wassignificantlydecreased(>2-fold),whereastheexpressionofmiRNA-134wassignificantlyincreased(>4-fold)intheamnioticfluidofratswithfetusesmodelingspinabifida.Theseresultswerevalidatedusingreal-timequantitativereverse-transcriptionpolymerasechainreaction.HierarchicalclusteringanalysisofthemicroarraydatashowedthatthesedifferentiallyexpressedmiRNAscoulddistinguishfetusesmodelingspinabifidafromcontrolfetuses.OurbioinformaticsanalysissuggestedthatthesedifferentiallyexpressedmiRNAswereassociatedwithmanycytologicalpathways,includinganervoussystemdevelopmentsignalingpathway.ThesefindingsindicatethatfurtherstudiesarewarrantedexaminingtheroleofmiRNAsthroughtheirregulationofavarietyofcellfunctionalpathwaysinthepathogenesisofspinabifida.Suchstudiesmayprovidenoveltargetsfortheearlydiagnosisandtreatmentofspinabifida.
简介:Receptortyrosinekinases(RTKs)suchastheepidermalgrowthfactorreceptor(EGFR)regulatecellularhomeostaticprocesses.EGFRactivatesdownstreamsignalingcascadesthatpromotetumorcellsurvival,proliferationandmigration.DysregulationofEGFRsignalingasaconsequenceofoverexpression,amplificationandmutationoftheEGFRgeneoccursfrequentlyinseveraltypesofcancersandmanybecomedependentonEGFRsignalingtomaintaintheirmalignantphenotypes.Consequently,concertedeffortshavebeenmountedtodeveloptherapeuticagentsandstrategiestoeffectivelyinhibitEGFR.However,limitedtherapeuticbenefitstocancerpatientshavebeenderivedfromEGFR-targetedtherapies.Awell-documentedobstacletoimprovedpatientsurvivalisthepresenceofEGFR-inhibitorresistanttumorcellvariantswithinheterogeneoustumorcellmasses.Here,wesummarizethemechanismsbywhichtumorsresistEGFR-targetedtherapiesandhighlighttheemergingroleofmicroRNAs(miRs)asdownstreameffectormoleculesutilizedbyEGFRtopromotetumorinitiation,progressionandthatplayaroleinresistancetoEGFRinhibitors.WealsoexamineevidencesupportingtheutilityofmiRsaspredictorsofresponsetotargetedtherapiesandnoveltherapeuticagentstocircumventEGFR-inhibitorresistancemechanisms.
简介:AbstractIn recent years, an increasing number of young women have been diagnosed with cancer, including some nulliparous women. Therefore, many young patients with early-stage cancer desire to preserve fertility after cytotoxic oncological treatments. It is important to develop a multidisciplinary approach to achieve the best outcomes for each patient. On the other hand, there has been a sharp increase in microRNAs (miRNAs) as potential biomarkers for the diagnosis, prognosis, and evaluation of treatment efficacy of several diseases. MiR-543 has been reported to affect the pathogenesis and progression of diseases via complex mechanisms. Understanding the regulatory role of miR-543 may aid comprehension of the pathogenesis and treatment of a broad range of diseases. Therefore, we provide an overview of the biogenesis, function, and role of miR-543 in various systems. These results shed light on the anticancer and endometrial protection role of miR-543 in young patients with gynecologic tumors and highlight the clinical potential of miR-543-based applications and related challenges.
简介:摘要角膜新生血管可继发于多种眼部疾病,是影响角膜透明度的主要原因之一。微小RNA(microRNA, miRNA)与病理性角膜新生血管密切相关,可通过调控多种细胞因子的表达和信号传导途径影响角膜新生血管生成。抑制角膜新生血管的miRNA包括miR-184、miR-204,促进角膜新生血管的miRNA包括miR-126、miR-132、miR-21和miR-27a/b。调控这些miRNA有望成为角膜新生血管的治疗方法。对促进角膜血管新生的miRNA,可使用反义miRNA寡核苷酸antagomir抑制内源性miRNA作用。(国际眼科纵览,2020, 44: 192-196)
简介:Epithelialovariancancer(EOC)istheleadingcauseofdeathamongallgynecologicalmalignancies.Despitethetechnologicalandmedicaladvancesoverthepastfourdecades,suchasthedevelopmentofseveralbiologicalmarkers(mRNAandproteinsbiomarkers),themortalityrateofovariancancerremainsachallengebecauseofitslatediagnosis,whichisspecificallyattributedtolowspecificitiesandsensitivities.Underthiscompulsivescenario,recentadvancesinexpressionbiologyhaveshiftedinidentifyinganddevelopingspecificandsensitivebiomarkers,suchasmicroRNAs(miRNAs)forcancerdiagnosisandprognosis.MiRNAsareanovelclassofsmallnon-codingRNAsthatderegulategeneexpressionattheposttranscriptionallevel,eitherbytranslationalrepressionorbymRNAdegradation.Thesemechanismsmaybeinvolvedinacomplexcascadeofcellulareventsassociatedwiththepathophysiologyofmanytypesofcancer.MiRNAsareeasilydetectableintissueandbloodsamplesofcancerpatients.Therefore,miRNAsholdgoodpromiseaspotentialbiomarkersinovariancancer.Inthisreview,weattemptedtoprovideacomprehensiveprofileofkeymiRNAsinvolvedinovariancarcinomatoestablishmiRNAsasmorereliablenon-invasiveclinicalbiomarkersforearlydetectionofovariancancercomparedwithproteinandDNAbiomarkers.