简介:Theworldeconomywassuffereddramaticallyin2008,becauseofthefinan-cialturnaoil.Itispredictedthatinthenextyears,Chinaeconomicgrowthwillslowdown,and2009wouldnotbethebottom,buttheeconomyfunda-mentalisstillhealthy.Asoft'U'turncanbeexpectedinthefuture.Macro-economyTwoofChinaeconomicgrowthpillarengines,exportsectorandreal-estateindustryhavebeensignificantlyinfluencedbytheglobalfinancialcrisis,trendsofthistwosectorsshowthatChinacouldnotbeanexceptioninthiseconomictur-moil.Theglobaleconomicturmoilhasasignificantnegativeimpactontheexportgrowth,anddomesticimportsalsoslowdownsincedomesticdemandhasbeenweaken,(SeeChart1)Asoneofthepillarindustry,realestateindustrysufferedalotundernationaldeclinelastyear,withanationalaveragedropof4.7percentonhousingprice.(SeeChart2)Domesticfixed-assetinvestmentgrowthhasalsosloweddown.Chinabusinesscyclesignalindexshowsthatthemacro-economicshasbeenslowingdown,andaccordingtothehistoryofChina'seconomy,thisdownturnwouldlastatleast3years,2009wouldnotbethecyclebottom.(SeeChart3)Thebusinesscyclesignalindexisdecliningfrom117.3inMayto94.7inOcto-ber,thefirsttimetodropunder100from1992,meaningthemacroeconomichasbeenslowingdown,butisstillinthestablezonesofar.LookingbackatthehistoryoverChina'spast30years,economicgrowthcycli-caldownturnwouldlastatleast3years.StandardCharteredBank'slatestreportreducedChinaGDPforecastfor2008to9.6percent,apoor7.9percentin2009,andevenlowerat7.1percentfor2010.(SeeChart4)ChinaisbelievedtobeshieldedfromchaoslikeUS,andfinancialwoeshavelimitedimpactonChina.Thecountryhadrichresourcereserves,greatmarketpo-tential,vigorousenterprisesandthegovernmenthadstrongmacro-controlabilities.Themajorindicatorsareinhealthycondition,whichcanhelpChinagovernmenttofighttheeconomyturmoilin2009.
简介:TheRepublicPartywoncomprehensivevictoryinthe2004U.S.generalelection.Notonlydiditkeeppresidency,expandseatsintheSenateandtheHouseofRepresentatives,italsoachievedcertainprogressingovernors'elections.ThevictoryinelectionbytheRepublicPartyreflectsthefactthatAmericasocietypaysutmostconcerntosecurityandthemainstreamofthetrendofthoughtisconservative.
简介:ThemaincauseofBush'ssuccessintheelectionandbeingre-electedPresidentisthattheRepublicansanditsconservatistideologyprevailsinAmerica.U.S.presidentialelectioniscontentionbetweenthecandidatesinpersonalcapacity,morality,experience,wealthandfamilyaswellascontentionbetweenthetwoparties.In2004election,BushisnotadvantageoustoKerryinpersonalfactors,butinthepartyitself.TheRepublicanshaveobviousadvantagestotheDemocraticParty.Themajorsourceoftheadvantagescomefromconservatistideology.
简介:摘要目的应用无血清培养基分离U2OS人骨肉瘤肿瘤干细胞,并鉴定相关肿瘤标志物表达。方法体外培养人骨肉瘤U2OS细胞株和U2OS人骨肉瘤肿瘤干细胞,免疫荧光检测U2OS人骨肉瘤肿瘤干细胞球中CD105表达。采用免疫印迹法检测人骨肉瘤U2OS细胞株和U2OS人骨肉瘤肿瘤干细胞Nanog和Oct4蛋白表达。采用半定量PCR检测人骨肉瘤U2OS细胞株和U2OS人骨肉瘤肿瘤干细胞SOX2、Nanog和Oct4mRNA的表达。结果荧光显微镜下可见,U2OS人骨肉瘤肿瘤干细胞球中部分细胞CD105呈阳性表达。免疫印迹检测结果显示人骨肉瘤U2OS细胞株的Nanog和Oct4蛋白相对表达量为0.853±0.404和0.603±0.153,均明显低于U2OS人骨肉瘤肿瘤干细胞0.973±0.305和0.827±0.322(均p<0.05)。半定量PCR检测结果显示U2OS人骨肉瘤肿瘤干细胞SOX2、Nanog和Oct4mRNA的相对表达量为0.190±0.027、0.178±0.025、0.170±0.021,均明显高于人骨肉瘤U2OS细胞株0.032±0.009、0.086±0.016、0.019±0.006(均p<0.05)。结论人骨肉瘤U2OS细胞株中存在肿瘤干细胞,无血清培养可用于分离培养人骨肉瘤肿瘤干细胞。