简介:摘要家庭内部56天内多人毒鼠强中毒较少见。该文报道5例患者因误服人为毒鼠强蓄意投毒食物引起毒鼠强中毒,主要临床表现为头痛,头晕、乏力、恶心、呕吐、四肢阵发性强直性抽搐、昏迷、呼吸衰竭,发作时全身抽搐、口吐白沫、小便失禁、意识丧失,实验室检查毒鼠强阳性,结合患者的临床表现以及特异性实验室检查,排除其他疾病,毒鼠强中毒诊断成立。本组病例因服毒病史不明确而误诊3例,误诊率高。本文所提5例患者3例死于呼吸衰竭,2例临床治愈。应提请广大临床医生注意人为投毒引起的毒鼠强中毒因病史不清,发病急,特别容易引起患者死亡,要及时确诊、早期洗胃、有效地控制惊厥及二巯基丙磺酸钠的应用。
简介:AbstractBackground:Epithelial to mesenchymal transition (EMT) is a key process in determining distant metastasis and intra-hepatic dissemination of hepatocellular carcinoma (HCC). Follistatin (FST) family members are considered to be an attractive therapeutic targets and prognostic indicators in cancers. As a derivative of FST, Follistatin Like 5 (FSTL5) may play a similar role in HCC cells. This study aimed to investigate the expression and function of FSTL5 in HCC and its role in EMT.Methods:FSTL5, E-cadherin and vimentin in HCC, and paracancerous tissues were detected by immunohistochemistry. Correlation of FSTL5 expression with overall survival was assessed. The proliferation and invasion of HCC cell lines SK-Hep1 and MHCC-LM3 were analyzed by cell counting kit-8 and Transwell assays. The expression of FSTL5, E-cadherin, and vimentin in HCC cells was examined by polymerase chain reaction and Western blot analysis. T-test was used to analyze the difference in proliferation and invasion ability between groups. The Spearman rank correlation test was used to detect the correlation between the expression of FSTL5 and E-cadherin or vimentin.Results:The expression of FSTL5 in HCC was lower than that in paracancerous tissues (9.97% vs. 82.55%, χ2 = 340.15, P < 0.001). Patients with high FSTL5 expression had a better prognosis (χ2= 8.22, P= 0.004) and smaller tumor diameter (χ2 = 45.52, P < 0.001), less lymph node metastasis (χ2 = 5.58, P= 0.02), earlier tumor node metastasis stage (χ2 = 11.29, P= 0.001), a reduced number of tumors (χ2 = 5.05, P= 0.02), lower alpha-fetoprotein value (χ2 = 24.36, P < 0.001), more probability of hepatitis carrying (χ2 = 40.9, P < 0.001), and better liver function grade (χ2 = 5.21, P = 0.02). Immunohistochemistry showed that FSTL5 expression in HCC tissues was positively correlated with E-cadherin expression (r = 0.38, P < 0.001) and negatively correlated with vimentin expression (r = -0.385, P < 0.001). Furthermore, over-expression of FSTL5 up-regulated the expression of E-cadherin and down-regulated the expression of vimentin in SK-Hep1 (negative control [NC] vs. FSTL5-interfering group [Lv-FSTL5]: E-cadherin [t= 45.03, P < 0.001], vimentin [t= 67, P < 0.001]) and MHCC-LM3 (NC vs. Lv-FSTL5: E-cadherin [t = 50, P < 0.001], vimentin [t = 72.75, P < 0.001]) cells at mRNA level. The same as protein level. In addition, the over-expression of FSTL5 inhibited the proliferation (NC vs. Lv-FSTL5: SK-Hep1, 3 d [t = 7.324, P = 0.018], 4 d [t = 6.23, P = 0.021], 5 d [t= 10.21, P= 0.003]; MHCC-LM3, 3 d [t= 4.32, P= 0.037], 4 d [t= 7.49, P= 0.012], 5 d [t= 9.3661, P = 0.009]) and invasion (NC vs. Lv-FSTL5: SK-Hep1, t= 21.57, P < 0.001; MHCC-LM3, t= 18.04, P < 0.001) of HCC cells.Conclusions:Down-regulation of FSTL5 may contribute to EMT of HCC, and FSTL5 is a potential target in the treatment of HCC.
简介:摘要目的调查四川省自贡市6个月~6岁儿童缺铁性贫血的状况。方法测定2017年5月至2019年11月在自贡市妇幼保健院儿童保健门诊进行健康体检的2 092例6个月~6岁儿童的血红蛋白值,分析儿童缺铁性贫血的影响因素。结果缺铁性贫血检出率为7.55%,以轻度贫血为主;男童、女童缺铁性贫血检出率比较差异未见统计学意义(P>0.05)。6~12个月组儿童缺铁性贫血检出率最高,不同年龄组间缺铁性贫血检出率比较差异有统计学意义(P<0.05)。母亲孕期贫血、母亲孕期未合理补铁、低出生体质量、早产、母亲文化程度低及未合理添加辅食是儿童缺铁性贫血的相关因素。结论儿童定期健康检查非常重要,应早期发现、早期诊断、早期治疗缺铁性贫血,以降低贫血对儿童健康的危害。
简介:目的探讨整合素αvβ6通过细胞外调节蛋白激酶介导胃癌细胞对5-氟尿嘧啶的耐受作用.方法胃癌AGS细胞培养至对数生长期后分4组处理.(1)对照组:向细胞悬液加入含5-氟尿嘧啶的培养基培养24h.(2)10D5组:向细胞悬液内加入0.1g/L鼠抗人αvβ6单克隆抗体10D5,培养6h后换液加入含5-氟尿嘧啶的培养基培养24h.(3)IgG2a组:加入10D5对照剂IgG2a后用5-氟尿嘧啶处理,余同10D5组.(4)PD98059组:向细胞悬液内加入含5-氟尿嘧啶和20μmol/L细胞外调节蛋白激酶抑制剂PD98059的培养基培养24h.MTT法检测细胞增殖,流式细胞仪检测细胞凋亡,Westernblot检测Bcl-2和caspase-3蛋白的表达情况.采用单因素方差分析和LSD检验.结果对照组、10D5组、IgG2a组和PD98059组的抑制率分别为28.1%±2.7%、84.5%±1.6%、31.4%±5.2%和86.7%±5.2%,组间比较差异有统计学意义(F=342.5,P〈0.05);凋亡率分别为6.6%±1.4%、30.6%±2.4%、8.1%±1.3%和36.0%±4.0%,组间比较差异有统计学意义(F=105.4,P〈0.05).caspase-3和Bcl-2的表达水平组间比较,差异有统计学意义(F=292.1,181.6,P〈0.05).结论整合素αvβ6可通过细胞外调节蛋白激酶介导胃癌细胞对5-氟尿嘧啶产生耐受.