简介：Overthedecade,ChineseBiomedicalEngineeringhasmaderemarkleprogressandaccumulatedmuchexperience.Animportantproblemfacingusishowtotaketheroadaheadofus.Firstofall,weshouldchangeourconventionalconceptandheadforthenewroad.Aboutthreeyearsago,wemadeaspecialreviewontheconnotationandchar-acteristicsofbiomedicalengineeringandpointedoutthatBiomedicalEngineeringwas

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简介：Theapplicationofhumanhomografthasbeenpopularincardiovascularsurgeryandshownperfecteffects.Butthedifficultytogetgraftgreatlylimiteditsclinicalap-plication.Thethawedhomograftcannotbeusedinsomecasessinceitisnotclearwhetheritcanbere-cryopreservedwithoutdamage.Weconductedthepresentstudytoknowthechangesinviabilityofthehomograftafterrefreezinginsixhumanpul-

简介：PACScanbeappliedinthenetworkarchitectureofClient/Server(C/S)andBrowser/Server(B/S)incurrentapplicationofhospitalinformationsystem.PACSViewerofB/Smodelisrealizedbymeansofthebrowsercommonly,butwebbrowserdoesnotsupportDICOMstandard.Inthisarticle,howtouseJavaApplettorealizethedisplayandoperationsofDICOMimageswillbediscussed.ThelightweightmethodtoviewDICOMimagesisveryfitfortelemedicaltreatment,andthesituationsnotrequirestrongabilityinimageoperations。

简介：Osteoblastsofratculturedinvitrowerestimulatedwithpulsed50Hzelectromagneticfieldandbasicfibroblastgrowthfactor(bFGF).TheMTTmethod,flowcytometryandhistochemistrystainingwereusedtodetectcellproliferation,cellcycleandalkalinephosphatase.Theresultsindicated:afterstimulatedby1mTelectromagneticfield,thecellsaremoreabundant,havemoreSphasepercentages,2mTelectromagneticfieldhavenoevidenteffectoncells'growth;comparedwithelectromagneticfield,thecellsstimulatedbybFGFaremoreabundantandhavelargerSphaseratios.ElectromagneticfieldandbFGFhavenoeffectoncells,alkalinephosphatase.Therefore,weconcludedthatelectromagneticfieldcanenhanceosteoblastsgrowthlikesomegrowthfactorsuchasbasicfibroblastgrowthfactor,andtheosteoblasts',characteristicswasnotchanged.

简介：Bodysurfacepotentialmapping(BSPM)isarecentlydevelopednoninvisivecar-dioelectrophysiologicdiagnostictechnique.Thepurposeofthepresentstudyistoe-valuatetheefficacyandsignificanceofpercutaneoustransluminalcoronaryangioplas-ty(PTCA)usingaBSPM-Ⅲmodelcomputersystem.7patientswitheoronaryheartdiseasewerestudiedpriortoandfollowingPTCAusing98leadsBSPMwithspecial

简介：ＴＨＥＡＰＰＬＩＥＤＳＴＵＤＹＯＦＴＨＥＴＯＴＡＬＨＩＰＲＥＰＬＡＣＥＭＥＮＴＦＯＲＴＨＥＡＤＵＬＴ＇ＳＣＯＮＧＥＮＩＴＡＬＤＩＳＬＯＣＡＴＩＯＮＯＦＴＨＥＨＩＰＡＴＴＨＥＰＲＩＭＡＲＹＡＣＥＴＡＢＵＬＵＭＴＨＥＡＰＰＬＩＥＤＳＴＵＤＹＯＦＴＨＥＴＯ...

简介：Objective:ThispaperistoexploreamethodoftransferringhumanSDF-1anditsmutantSDF-1/54intrakinegeneintoCOS-7cellsfordeterminingtheirexpressionandsubcelluarlocalizationofthefusionprotein.Thiscouldofferfeasibilityforinhibitingthemetastasisofmalignanttumorsbyphonotypicknockoutforblockingfunctionalexpressionofreceptoronthecell-surface.Methods:AmplifythetargetgenewithPCRfromtheconstructedplasimidSDF-WT-Gly×4-Dec/PET-30a(+)withaC-terminalretentionsignalfragmentKDEL.AfterthepcDNA3.1/SDF-1/KDEL,pcDNA3.1/SDF-1/54/KDEL,pEGFP/SDF-1/KDELandpEGFP/SDF-1/54/KDELeukaryoticexpressionvectorswereconstructedandtheDNAsequencewasaccurate,theyweretransferredintoCOS-7cellswithliposome.Theexogenousexpressionswereobserved,fusionproteinSDF-1/HisandSDF-1/54/HiswereconfirmedbyWesternblot,andtheSDF-1/EGFPandSDF-1/54/EGFPweredeterminedbyLaserScanningConfocalMicroscopy.Results:Fourexpressionvectorswereconstructedsuccessfully,thefusionproteinSDF-1/KDEL/HisandSDF-1/54KDEL/HisexpressedinCOS-7cells.SubcelluarlocalizationanalysisshowedthatSDF-1/KDEL/EGFPandSDF-1/54/KDEL/EGFPwerelocatedmainlyinendoplasmicreticulum.Conclusion:FourexpressionvectorspcDNA3.1/SDF-1/KDEL,pcDNA3.1/SDF-1/54/KDEL,pEGFP/SDF-1/KDELandpEGFP/SDF-1/54/KDELwereconstructedsuccessfully,whichcouldexpressineukaryoticcellandlocatemainlyintheendoplasmicreticulum.

简介：通过短跑和中长跑运动员在300W负荷时进行30s全速踏车试验,结果显示前15s的踏车速度与运动员的速度能力呈正相关,提示:300W负荷时进行30s全速踏车试验,可作为评定运动员速度能力的一种简易方法

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简介：Gliomahasthehighestincidenceinthebraintumors.Thoughtreatedwithsurgicalresection,externalbeamradiationtherapy,andsystemicchemotherapy,patientswithgliomahavepoorprognosisbecauseofgliomarecurrence.