简介:目的:研究在高糖条件下,从海藻中萃取的新型多糖化合物对高糖诱导的视网膜色素上皮(RPE)细胞异常增殖的保护作用.方法:将体外培养的RPE细胞分为空白组、高糖组和多糖化合物组,空白组为正常RPE细胞培养液,高糖组为含30mmol/L葡萄糖的培养液,多糖化合物组为含30mmol/L葡萄糖和200mg/L多糖化合物的培养液.应用MTT法测量36h内不同时间点(6,12,24和36h)高糖以及多糖化合物对RPE细胞增殖影响.结果:高糖导致RPE细胞异常增殖,多糖化合物组RPE的细胞异常增殖明显得到保护,与高糖组比较差异具有统计学意义(P〈0.01).结论:从海藻中萃取的新型多糖化合物可以明显保护高糖所导致的RPE细胞的异常增殖.
简介:目的:探讨中老年人膳食碳水化合物及脂肪摄入量与年龄相关白内障(age-relatedcataract,ARC)危险性之间的关系。方法:采用以医院为基础的病例对照研究方法。病例由45~85岁的360例白内障患者组成,对照由同期入住相同医院的360例非白内障的患者组成,采取1∶1匹配。采用自行设计的调查表对研究对象进行调查,内容包括人口学特征、生活方式、以及既往史等,同时还采用一份有效的半定量化食物频率调查表(FFQ),获取研究对象膳食营养素的摄入情况。应用多因素Logistic回归分析估计碳水化合物及脂肪摄入量与ARC关联的比值比(OR)及其相应的95%可信区间(CI)。结果:调整多种潜在性混杂因素后,碳水化合物摄入量增加可使发生ARC的危险性升高,与摄入量为最低四分位数的对照相比较,摄入量为最高四分位数的病例发生ARC的OR为2.161(95%CI:1.107~5.016,P〈0.001)。同样,多不饱和脂肪酸摄入量也与ARC呈正相关,摄入量为最高四分位数的病例发生ARC危险性是最低四分位数对照的3倍(OR=2.989,95%CI:1.764~5.062,P〈0.001)。总脂肪摄入量与ARC无关(P=0.778)。结论:膳食碳水化合物及多不饱和脂肪酸摄入量增加可使发生ARC的危险性升高,合理营养、平衡膳食可能有助于预防ARC。
简介:目的探讨制霉菌素泡腾片与湿润烫伤膏水浴后混合物(简称制霉菌素油剂)的广谱抗真菌、改善耳道内环境、恢复耳道上皮分泌及自洁功能的效果。方法以60例行真菌涂片滴入氢氧化钾镜于下检查确诊为真菌性外耳道炎的患者为研究对象,2周为1个疗程,不得中途退出。常规清洁耳道,待耳道干燥后涂布制霉菌素油剂,治疗3~5次后评估患者耳道不适症状的改善、分泌物质与量的变化、上皮充血糜烂的恢复、真菌的检测分型与培养药敏变化。结果药剂分布均匀,耳内分泌物易排出,患者的舒适度与上皮功能恢复改善快。结论制霉菌素有广谱杀真菌效果,合用湿润烫伤膏的油剂可改善耳道内环境。制霉菌素油剂治疗真菌性外耳道炎符合耳道生理病理治疗需求,效果良好。
简介:目的:观察小牛血去蛋白提取物眼用凝胶对复发性翼状胬肉术后角膜切口修复的应用疗效。方法:将68例68眼行复发性翼状胬肉术后发生角膜切口水肿的患者随机分为2组,每组34例。对照组常规应用双氯芬酸钠滴眼液加抗生素滴眼液同时加用贝复舒滴眼液交替点眼,4次/d。治疗组除每日应用双氯芬酸钠滴眼液加抗生素滴眼液交替点眼外,使用小牛血去蛋白提取物眼用凝胶,4次/d,疗程1mo,于用药前和用药后3,7,14d;1mo分别对患者主觉症状、体征、角膜切口修复情况进行对比分析。结果:复发性翼状胬肉术后3,7,14d,治疗组角膜切口水肿较对照组明显消退,切口修复较快,主觉症状在短时间消退,术后1mo,两组无显著性差异。结论:小牛血去蛋白提取物眼用凝胶对复发性翼状胬肉术后角膜切口的早期修复起效时间短、舒适度好、安全性良好,对减少术后感染性角膜病的发生及术后角膜散光有良好的作用。
简介:目的:评价超声乳化白内障吸除联合人工晶状体植入术后人工晶状体眼的波前像差,讨论不同设计的人工晶状体对术后人工晶状体眼波前像差的影响。方法:选择年龄相关性白内障患者62例(69眼),年龄41~84(平均63.4±4.0)岁。其中男24例(28眼),女38例(41眼),右眼38例,左眼31例。随机平均分为3组,使年龄性别相匹配。其中A组植入三片式人工晶状体(ACRYsof^@MA60BM),B组植入一片式人工晶状体(ACRYsof^@SA60AT),C组植入蓝光滤过型一片式人工晶状体(ACRYsof^@SN60AT)。同一术者,同一超声乳化仪(Alcon^@INFINITIVISIONSYSTEM)和手术显微镜(CarlZeissStativS88),术中均采用角膜曲率最高经线上宽3.2mm长1.75mm的角巩膜缘隧道切口。术后1mo使用客观型波前像差仪(NidekOPD-scanARK-10000)进行波前像差检测,得出总体高阶像差的均方根(RMSh)。结果:A组三片式(ACRYsof^@MA60BM)的RMSh平均达到0.702±0.090μm,B组一片式(ACRYsof^@SA60AT)的RMSh平均达到0.529±0.067μm,C组蓝光滤过型一片式(ACRYsof^@SN60AT)的RMSh平均达到0.566±0.066μm。三组比较采用单向方差分析,5.0mm瞳孔大小时A组的总体像差值最高(P〈0.01),其余两组没有显著性差异(P=0.126)。组间差异有统计学意义。结论:人工晶状体襻的设计对超声乳化白内障吸除联合人工晶状体植入术后人工晶状体眼的像差有明显影响,但光学区染色对单色像差的影响无统计学差异。这一结果对进一步完善白内障手术以及人工晶状体材料和设计的改善提供了有意义的信息。
简介:目的探讨一组特殊设计镜片对周边屈光度、周边清晰视力范围和主观感受的影响。方法3例(4眼)被试者,年龄22-31岁,屈光度-2.0D,按随机顺序分别在单眼配戴普通非球面镜片、成长乐镜片、视特保大、中、小光区镜片的情况下进行周边屈光度、周边清晰视力范围测量和主观评分,主观评分包括远距和近距戴镜舒适度、清晰度和接受度。结果配戴5种镜片时,被试眼相对周边屈光度均为远视状态,远视度数随周边角度增加而增加。配戴普通非球面镜片和视特保大光区镜片时周边远视度数最大,视特保中光区镜片其次,成长乐镜片和视特保小光区镜片最小;配戴普通非球面镜片和视特保大光区镜片时的周边清晰视力范围最大,其次是视特保中光区镜片,最小的是成长乐镜片和视特保小光区镜片;远距评分为普通非球面镜片最高,视特保大光区镜片其次,然后为视特保中光区镜片,成长乐镜片及视特保小光区镜片最差;近距评分视特保大光区镜片接近普通非球面镜片,视特保中光区镜片和成长乐镜片其次,视特保小光区镜片最差。结论不同设计的中周部加光镜片对改变周边屈光度的作用存在差异,其配戴后的顺应性与镜片改变周边屈光度的程度呈负相关。
简介:Thekeratoprosthesis(KPro;artificialcornea)isaspecialrefractivedevicetoreplacehumancorneabyusingheterogeneousformingmaterialsfortheimplantationintothedamagedeyesinordertoobtainacertainvision.Themainproblemsofartificialcorneaarethebiocompatibilityandstabilityofthetissueparticularlyinpenetratingkeratoplasty.Thecurrentstudiesoftissue-engineeredscaffoldmaterialsthroughcomprisingcompositesofnaturalandsyntheticbiopolymerstogetherhavedevelopedanewwaytoartificialcornea.Althoughawideagreementthatthelong-termstabilityofthesedeviceswouldbegreatlyimprovedbythepresenceofcorneacells,modificationofkeratoprosthesistosupportcorneacellsremainselusive.Mostofthestudiesoncornealsubstratematerialsandsurfacemodificationofcompositeshavetriedtoimprovethegrowthandbiocompatibilityofcorneacellswhichcannotonlyreducethestimulusofheterogeneousmaterials,butalsomoreimportantlycontinuousandstablecorneacellscanpreventthedestructionofcollagenase.Thenecrosisofstromaandspontaneousextrusionofthedevice,allowformaintenanceofaprecornealtearlayer,andplaytheroleofensuringagoodopticalsurfaceandresistingbacterialinfection.Asaresult,improvementincornealcellshasbeenthemainaimofseveralrecentinvestigations;someefforthasfocusedonbiomaterialforitswellbiologicalpropertiessuchaspromotingthegrowthofcorneacells.Thepurposeofthisreviewistosummarythegrowthstatusofthecornealcellsaftertheimplantationofseveralartificialcorneas.
简介:AIM:Todescribethedesignandpreliminaryresultsofthehospitalbasedepidemiologicalstudyfordiabeticretinopathy(HBESDR),anongoingepidemiologicalstudytoestimatetheprevalenceofdiabeticretinopathy(DR)andtoelucidatetheclinical,anthropometric,biochemicalandanyotherriskfactorsassociatedwithdiabeticretinopathy.METHODS:Totally2000diabeteswillberecruitedfromtheDiabeteseyeclinicintheFirstAffiliatedHospitalofChinaMedicalUniversity.AllsubjectsunderwentbloodsugarestimationandOralGlucoseToleranceTesttodiagnosediabetes.Alldiabeteswouldundergocompletequestionnaire,acomprehensiveeyeexamination.Bloodandurinewouldbecollectedforbiochemicalinvestigations.AllfundusphotographsforanyDRwillbegraded.Participantswhoneedtreatmentwillbesenttotheophthalmicclinicandfollow-upintervalprogramforallsubjectswillbesuggested.Acomputerizeddatabaseiscreatedfortherecords.RESULTS:Todate,1174diabeteshavebeenrecruited,therewere350(29.81%)DRinalldiabetes,mostofthemwerewithmildnon-proliferativediabeticretinopathy(NPDR)(139,39.71%);71(20.29%)moderateNPDR,66(18.86%)severeNPDR,74(21.14%)proliferativediabeticretinopathy(PDR).Females,longerdurationofdiabetes,familyhistoryofdiabetesandhypertensionhadastatisticallysignificantincreaseinriskofanyDR.CONCLUSION:ThestudyisexpectedtoprovideanestimateoftheoverallprevalenceofDRandtheprevalencewithdifferentdurationofdiabetesandalsoabetterunderstandingoftheriskfactorsassociatedwithDR.
简介:AIM:Todemonstratethemorphologyandstructureofinvitroreconstructedtissue-engineeredhumancornealepithelium(TE-HCEP)withseedercellsfromanuntransfectedHCEPcellline.·METHODS:TheTE-HCEPswerereconstructedinvitrowithseedercellsfromanuntransfectedHCEPcellline,andscaffoldcarriersofdenudedamnioticmembrane(dAM)inair-liquidinterfaceculturefor3,5,7and9days,respectively.Thespecimenswereexaminedwithhematoxylin-eosin(HE)stainingofparaffin-section,immunocytochemicalstaining,scanningandtransmissionelectronmicroscopy.·RESULTS:DuringinvitroreconstructionofTE-HCEP,HCEPcellsformeda3-4,6-7and8-10layersofanHCEP-likestructureondAMsinair-liquidinterfaceculturefor3,5and7days,respectively.Butthecellsdeceasedto5-6layersandthestructureofstraifiedepitheliumbecamelooseatday9.Andthecellsmaintainedpositiveexpressionofmarkerproteins(keratin3andkeratin12),cell-junctionproteins(zonulaoccludens-1,E-cadherin,connexin43andintegrinβ1)andmembranetransportproteinofNa+-K+ATPase.TheHCEPcellsinTE-HCEPwererichinmicrovillionapicalsurfaceandestablishednumerouscell-cellandcell-dAMjunctionsatday5.·CONCLUSION:ThemorphologyandstructureofthereconstructedTE-HCEPweresimilartothoseofHCEPinvivo.TheHCEPcellsinthereconstructedTE-HCEPmaintainedthepropertiesofHCEPcells,includingabilitiesofformingintercellularandcell-extracellularmatrixjunctionsandabilitiesofperformingmembranetransportation.TheuntransfectedHCEPcellsanddAMscouldpromisinglybeusedinreconstructionHCEPequivalentforclinicalcornealepitheliumtransplantation.