简介:【摘要】目的:分析荧光定量RT-PCR在流感病毒检测上的应用价值。方法:对23例确诊感染乙型流感病毒的咽拭子、相关机构提供的甲型流感病毒及乙型流感病毒进行检测分析,使用荧光定量RT-PCR检测,分析临床应用的价值及检测的准确率。结果:荧光定量RT-PCR检测乙型流感病毒呈阳性,且结果与其他病毒未见交叉情况。荧光定量RT-PCR检测不同浓度乙型流感病毒敏感度较高,检测的准确率是86.96%(20/23)。结论:荧光定量RT-PCR检测流感病毒的敏感度、准确性较高,可以作为疾病筛查的依据,建议推广应用。
简介:Thepurposeofthestudyistoestablishafluorescencequantitativereversetranscriptionpoly-merasechainresponse(FQ-RT-PCR)methodforthequantitativedeterminationofIL-2mRNAandIL-4mRNAinThcells,withwhichtheThcellsstatusofthepatientswithgynaecologicaltumorsandchronicrenalfailure(CRF)canbeanalyzed.IL-2cDNAandIL-4cDNAwereprepared,andtheplasmidpMD18carryingIL-2cDNAorIL-4cDNAfragmentwasconstructedandclonedasthetemplateforquantitativedetermination.Theprimersandprobeslabelledwith6-carboxy-fluorescein(FAM)and6-carboxy-tetrarnethylrhodamine(TAMRA)wereprepared,andtheexperimentalconditionswereoptimizedtosetuptheFQ-RT-PCRmethodforquantitativedeterminationofIL-2mRNAandEL-4mRNA.Thcellsenrichedfromperipheralbloodmononuclearcells(PBMCs)of20healthyvolunteers(HVs),16gynaecologicalbenign(GB)cases,18gynaecologicalmalignant(GM)tumorcasesand16chronicrenalfailure(CRF)patientsweretestedforIL-2mRNAandIL-4mRNAbyFQ-RT-PCR.Thehouse-keepinggeneβ-actinwasusedastheinternalcontrolgeneoftheexperiment.Thestandardcurveforlogconcentrationofseriesofquantitativetemplatesvsthresholdcycle(CT)wasestablishedbylinearregression,andthelinearrangewas102-107copies/μl.TheimprecisiontestshowedtheCVofinter-assayandintra-assayofahighcontentsamplebyFQ-RT-PCRwere7.8%and12.5%,respectively.TheCVofinter-assayandintra-assayofalowcontentsamplewere10.8%and19.5%,respectively.TheIL-2mRNAexpressionsinThofthepatientswithgynaecologicalmalignanttumor(comparedwiththeHVsandthepatientswithgynaecologicalbenigndisease)andinThoftheCRFpatients(comparedwiththeHVs)weredeclinedsignificantlyandatthesametimetheIL-4mRNAexpressionincreasedsignificantly(P<0.001).Asimple,sensitiveandaccurateFQ-RT-PCRmethodforthequantitativedetectionofIL-2mRNAandIL-4mRNAhasbeenestablished.TheIL-2mRNAandIL-4m
简介:【摘要】目的:分析重组织型纤溶酶原激活物(rt-PA)经皮静脉溶栓治疗急性缺血性脑卒中的临床应用价值。方法:将2018年11月01日至2020年03月01日在本院就诊的34例急性缺血性脑卒中患者视为研究对象,以双盲法划入参照组与研究组(n=17)。参照组常规药物治疗,研究组在此基础上使用重组织型纤溶酶原激活物(rt-PA)经皮静脉溶栓治疗,对比患者的治疗效果。结果:治疗后研究组患者的症状恢复率是94.12%,参照组的症状恢复率是76.47%,研究组与参照组对比有统计学意义(P<0.05)。研究组患者治疗期间出现1例并发症,参照组患者治疗期间出现4例并发症,研究组的并发症发生率相对较低(