Combiningtheinhibitedaptazymeandmolecularbeacon(MB),wedevelopedaversatilesensingstrategyforamplifieddetectionofadenosine.Inthisstrategy,theadenosineaptamerlinkstothe8-17DNAzymetoformanaptazyme.Ashortsequence,denotedasinhibitor,isdesignedtoformaduplexspanningtheaptamer–DNAzymejunction,whichblocksthecatalyticfunctionoftheDNAzyme.Onlyinthepresenceoftargetadenosine,theaptamerbindstoadenosine,thustheinhibitordissociatesfromtheaptamerportionoftheaptazymeandcannolongerformthestableduplexrequiredtoinhibitthecatalyticactivityoftheaptazyme.ThereleasedDNAzymedomainwillhybridizetotheMBandcatalyzethecleavageinthepresenceofZn2+,makingthefluorophoreseparatefromthequencherandresultinginfluorescencesignal.Theresultsshowedthatthedetectionmethodhasadynamicrangefrom10nmol/Lto1nmol/L,withadetectionlimitof10nmol/L.
