简介：BasedonAVISO(archiving,validationandinterpretationofsatellitedatainoceanography)datafrom1993to2010,QuikSCAT(QuickScatterometer)datafrom2000to2008,andArgodatafrom2003to2008,theinterannualvariabilityoftheGreatWhirl(GW)andrelatedmechanismsarestudied.ItshowsthattheoriginandterminationtimesoftheGW,aswellasitslocationandintensity,havesignificantinterannualvariability.TheGWappearedearliest(latest)in2004(2008)andvanishedearliest(latest)in2006(2001),withtheshortest(longest)durationin2008(2001).Itscenterwasmostsouthward(northward)in2007(1995),whiletheminimum(maximum)amplitudeandareaoccurredin2003and2002(1997and2007),respectively.TheGWwasweakeranddisappearedearlierwithitslocationtendingtobeinthesouthwestin2003,whilein2005itwasstronger,vanishedlaterandtendedtobeinnortheast.TheabnormalyearswereoftennotthesameamongdifferentcharactersoftheGW,andwerenotallcoincidentwithENSO(ElNi?o-SouthernOscillation)orIOD(IndianOceanDipole)events,indicatingtheverycomplexnatureofGWvariations.MechanisminvestigationsshowsthattheinterannualvariabilityofintraseasonalwindstresscurlinGWregionresultsinthatoftheGW.ThegenerationoftheGWiscoincidentwiththearrivalofRossbywavesattheSomalicoastinspring;theintensityoftheGWisalsoinfluencedbyRossbywaves.TheterminationoftheGWcorrespondswelltothesecondoneofthetoptwopeaksinthebaroclinicenergyconversionrateinGWregion,andtheintensityandthepositionoftheGWarealsocloselyrelatedtothetoptwobaroclinicenergyconversionrates.

简介：Inthisstudy,weexaminedtheeffectofelevatedtemperatureontheexpressionpatternsofgenes,i.e.,nacrein,irr,n16,n19,andhsp70inthepearloysterPinctadafucata.Theexperimentwascarriedoutat4temperatures,i.e.,20℃(ambient,control),24,28℃,and32℃.TheexpressionlevelsoftargetgenesinP.fucatawereassayedat0,6,24,48,and96hviareal-timepolymerasechainreaction.Resultsshowedthattheexpressionlevelsofnacreinandirrhadnosignificantvariationsamongdifferenttimepointsbelow28℃,butsignificantlyincreasedovertimeat32℃.Theexpressionlevelsofn16andn19didnotchangemarkedlyat20℃.Theformerincreasedsignificantlyat6hand24hwhilethelattersubstantiallydecreasedduring6–96hat24,28and32℃.Amongdifferenttemperatures,thelevelofn16wassignificantlylowerat20℃thanatothertemperaturesduring6–96h,andthelevelofn19significantlyvariedamongdifferenttemperaturesat48hand96h.Theexpressionlevelofhsp70wassignificantlyhigherat32℃thanat20,24and28℃at24h.TheseresultsdemonstratedthatelevatedtemperatureimpactedthephysiologicalprocessesofP.fucataandpotentiallyinfluenceditsadaptabilitytothermalstress.

简介：AgeopulseseismicsystemwasimplementedtoobtainnewinformationonunderlyingstratabeneaththeYellowRiverDelta.However,owingtotheslippingofseafloorandshallowwater(<25m),freesurface-relatedmultiplesareconflictedwiththeflatprimaries,whichmayinstructtheinterpretersinawrongwayandmakewrongconclusions.Owingtothevariationofmultipleperiods,standardanti-multipletechniquesmaylosetheirpower,suchaspredictivedeconvolution.Surface-relatedmultipleeliminationmethodsareintroducedtoattenuatethemultiplesbasedontheunderlyingwave-equationprinciples.Theresultsjustifiedthemethodonthesingle-channelgeopulseprofile,revealingtherealnatureofthesubsurface.

简介：Calmodulinisacalciumbindingproteinthatmodulatestheactivityofdiversegroupsofproteinincludingsomeproteinkinase,adenylatecyclasesandATPase.HereweusethetotalDNAofSonneratiaparacaseolarisasthetemplateofthepolymerasechainreaction(PCR).ThePCRprimershavebeendesignedandsynthesizedaccordingtothe5-and3-terminaloligonucleotidesequencesofCalmodulingeneofplantsinGenbankandligatedwithcloningvectorpBsk(+).Therecombinantcloneshavebeenobtainedfromtheselectedmedium.TheresultsofDNAsequencesanalysisshowthatthenucleotidesequencesofORFsharemorethan85%homologiesascomparedwiththoseofcalmodulingenesofseveralotherplants.Similartoriceandapple,theORFisinterruptedbyanintronbehindthe75thnucleotide.

简介：类Cylindrotheca与一些由海洋的硅藻的一个小组组成描述的种类。十一当Cylindrothecadosteriummorphologically与他们小子单元的ribosomalRNA(SSUrDNA)和在这决定的编码叶绿体的rbcL基因序列学习的原子编码从Jiaozhou海湾被获得，识别的硅藻孤立。有趣地，SSUrDNA的很高的顺序分叉和rbcL基因在这些之中被发现孤立，并且相对引起推出的氨酸的很少变化的rbcL基因的众多的核苷酸变化定序。分别地，种系发生的分析基于SSUrDNA和rbcL基因组织了isolates进6clades。SSUrDNA的种系发生的树放了Cylindrotheca一起孤立的所有，清楚地把他们分开成二个系。系我由十一C组成。和另一C在这研究获得的dosteriumisolates。dosterium孤立，但是某clades很好没被支持。系II包含了二C。dosterium孤立并且一C。纺锤形孤立。Cylindrotheca孤立进二well-definedlineages的也分开的rbcL基因的种系发生的分析。十一C。dosterium孤立形成了一个系，所有clades强烈被支持。SSUrDNA的统计比较显示在我是的系以内的平均距离比另外的微水藻种类的显著地高(P<0.01)。这些结果在C以内建议了神秘种类的存在。dosterium。

简介：六份ISSR教材被采用显示不同阶段的多型性和红水藻Gracilarialemaneiformis的性，并且他们中的二个，P1和P3，放大不同乐队模式。女配偶体的教材P1放大的ISSR模式与那oftetrasporophyte相同，但是从男配偶体的不同。教材P3生产的乐队，一个人对女配偶体特定。三片词法上类似的复叶能容易用ISSR技术被识别。二个特定的标记，SM1andSF3与男配偶体和女配偶体有关，被克隆并且定序。SM1的相应序列被发现编码假想蛋白质。没有能在GenBank被发现的SF3的相应顺序。

简介：为模仿的一个提高的数字模型二维不可压缩粘滞有弄歪的免费表面的流动被报导。数字模拟彻底地被执行CIP(抑制插值侧面)基于方法，它在纸被描述。捕获计划的一个更精确的接口，VOF/WLIC计划(VOF：Volume-of-Fluid；WLIC：称的线接口计算)，作为捕获方法的接口被采用。估计发达算法和它的通用性，测试问题的一种选择被检验，即方形的波浪繁殖，Zalesaks僵硬身体旋转，有或没有障碍的水坝碎问题，在在极端波浪和漂浮的身体之间的一辆激动的波浪坦克和相互作用的波浪sloshing。当数字结果与相比时，优秀协议被获得可得到分析，试验性，并且另外的数字结果。这些例子证明在捕获的免费表面的VOF/WLIC计划的使用做更好的结果并且另外建议基于CIP的模型能够预言怪胎波浪相关的现象。

简介：TheDiverGasRecoverySystem(DGRS)isanimportantaccessoryequipmentofthe300mSaturationDiveSystem.Thesystemfacilitatestherecyclingofthebreathinggasesfordivers,therefore,ithasgreateconomicbenefits.InApril1995,a300munmannedtestanda100mmannedtestontheHY2031DGRSwerecarriedoutsuccessively.Tenmonthslater,anothermannedtestontheHY2032DGRS

简介：EvolutionaryevidencesuggeststhatSox3,amemberofthehigh-mobility-group(HMG)familyoftranscriptionfactors,isanancestralprecursorofSryandisinvolvedinsexdeterminationsimilartoSry.However,thereislimitedinformationregardingtheSOX3geneoftheblackrockfish(Sebastesschlegeli).Inthisstudy,wefirstisolatedSOX3genefromthegonadsofS.schlegelibyhomologycloning.Thefull-lengthofS.schlegeliSOX3(SsSOX3)cDNAwas1386bp,comprisinga906-bpopenreadingframe,whichencodesapeptideshowing93.6%and93.9%homologywiththeSox3proteinsofEpinepheluscoioidesandOryziaslatipe,respectively.ComparisonofthecDNAsequenceoftheSsSOX3genewiththecorrespondinggenomicDNAfragmentrevealedthattheSsSOX3geneconsistsofasingleexon.PhylogeneticanalysisdemonstratedtheevolutionaryrelationshipofSsSOX3withotherknownSOXB1genesinfishandtetrapods.ThepromoterregioncontainsbindingsitesofseveraltranscriptionalfactorsthatmightparticipateintheregulationofSsSOX3expression.Quantitativereal-timePCRanalysisindicatedthatSsSOX3wasexpressedinalltheinvestigatedlarvaldevelopmentalstagesfrom1to35daysafterbirthandthelevelofexpressiongraduallydecreasedasthedevelopmentproceeded.SsSOX3exhibitedsexuallydimorphicexpressioninadultgonads,withhighexpressionintheovarybutlowexpressioninthetestis.InsituhybridizationrevealedthatSsSOX3wasstronglyexpressedinoocytesandfollicularcellsofovariesbutslightlyexpressedingermcellsoftesticulartissues.Therefore,thisstudysuggeststhatSsSOX3playsanimportantroleinoogenesisandovarydifferentiationinS.schlegeli.

简介：Onthebisisofdeterminingthethereelementsofthemocline(depth[upperbounddepth],thicknessandintensity)andthemaximumverticaltemperaturegradientofthesurveyingstation,thepapercalculatesthemeantemperatureoftheNanshadeep-watersurveyingstationwithintheupper-bounddepthlayerofthermoclineandthemeantemperaturebelowthelowerbounddepthofthermoclinebetweenthe300mand800mlayers,respectively.AnalysisindicatesthatthehorizontaldistributionofmeanseawatertemperatureshowsadistincttrendofthelowtemperatureseawaterslowlymovingfromtbenortheasttothesoutheastofNansha,whichseemstohavebeendrivenbytheNortheastMonsoon.Thelargertheverticaltemperatureradientis,thegreaterisitscapabilityofpreventingtheheatoftheupperseawaterfromdiffusingintothedeeperlayersontheverticaldirection.

简介：ThisstudyexaminedtheeffectofsalinityontheexpressionofNa+/K+-ATPase(NKA)α-subunitandvacuolar-typeH+-ATPase(V-ATPase)β-subunitgeneinthegillofLitopenaeusvannamei.Semi-quantitativereversetranscription-polymerasechainreaction(RT-PCR)assayshowedthattheexpressionofNKAα-subunitandV-ATPaseβ-subunitgenewassignificantlyinfluencedbysalinity.ItwasfoundthattheNKAactivitysignificantlyvariedwithsalinityintimeanddosedependentmanner;whereastheV-ATPaseactivitydidnot.TheabundanceofNKAα-subunitgenetranscriptincreasedrapidlywhenthesalinitydecreasedfrom26bto21,andslowlywhenthesalinitydecreasedfrom26to31withinthefirst24h.Whenthesalinitydecreasedfrom26to21,thetranscriptionofNKAα-subunitgeneingillepitheliumwashigherat12hthanthatat0h,whichwasconsistentwiththeresultofimmunoblottingassayofNKAα-subunit.Inaddition,salinityhadasignificanttime-anddose-dependenteffectontheconcentrationofbiogenicaminesinbothhemolymphandgill.Ascomparedtootherparameters,theconcentrationofdopamine(DA)and5-hydroxytryptamine(5-HT)variedindifferentpatternswhenthesalinitydecreasedfrom26to21orincreasedfrom26to31,suggestingthatDAand5-HTplayeddifferentregulatoryrolesinosmoticadaptionandmodulationofshrimpwhensalinityvaries.

简介：完全的mitochondrial细胞色素氧化酶子单元II(COII)基因ofPenaeinae虾Fenneropenaeuschinensis被克隆并且定序。基因为229氨酸是在长度和代码的688bp。它显示出83.2%，87.0%和83.8%顺序类似到Marsupenaeusjaponicus，Penaeus单音穿上并且Farfantepenaeusnotialis分别地。在鳕鱼ons的第三个位置的整个基因和那的A+T内容分别地是64.7%和78.2%。在F之间的种系发生的关系。chinensis和代表类Farfanatepenaeus，Marsupenaeus和Penaeus的三另外的种被分析。结果证明在四taxaranged从之中的基因距离0.1440～0.2005，超过与COI和部分16SrRNA基因估计的那些在Marsupenaeus，Litopenaeus和Melicertus之中定序，并且因此比在潜水艇类之中的价值大。COII基因比COI基因和部分16SrRNA基因的有更快的进化的率，这被建议了并且能在类或种水平被用于种系发生的分析。现在的学习的结果显示Farfantepenaeus，Fenneropenaeus，Marsupenaeus和Penaeus比亚属在更高种系发生的水平，它支持四个潜水艇类的种系发生的地位的举起的意见到类水平。

简介：Folliclestimulatinghormoneβ(FSHβ)ofJapaneseflounder(Paralichthysolivaceus)playsakeyroleintheregulationofgonadaldevelopment.ThisstudyaimedtoinvestigatemoleculargeneticcharacteristicsoftheFSHβgeneandelucidatetheeffectsofsinglenucleotidepolymorphisms(SNPs)ofFSHβonreproductivetraitsinJapaneseflounder.Weusedpolymerasechainreactionsingle-strandconformationpolymorphism(PCR-SSCP)andsequencingoftheFSHβgenein60individuals.WeidentifiedonlyanSNP(T/C)inthecodingregionofexon3ofFSHβ.TheSNP(T/C)didnotleadtoaminoacidchangesattheposition340bpofFSHβgene.StatisticalanalysisshowedthattheSNPwassignificantlyassociatedwithtestosterone(T)levelandgonadosomaticindex(GSI)(P<0.05).IndividualswithgenotypeTCoftheSNPhadsignificantlyhigherserumTlevelsandGSI(P<0.05)thanthatofgenotypeCC.Therefore,FSHβgenecouldbeausefulmolecularmarkerinselectionforprominentreproductivetraitinJapaneseFlounder.

简介：

简介：18SribosomalDNA基因(18SrDNA)序列(近似在长度的1300bp)从从与线虫大批从Qingdao海岸的内部潮汐的沉积收集的免费生活的海洋的线虫提取的DNA被放大特定的教材。PCR产品被克隆，重新放大，与Rsa消化了我和Hin6我限制endonucleases和分开的inagarose胶化。在17种限制碎片长度类型之中，打1，2和6盖住61.2%，分别地，当时，克隆的14.4%and9.3%分析了留下14仅仅盖住21克隆，它说明了15.1%总数。24代表性的克隆andphylogenetically被定序由指在RDP和GenBank数据库当前可得到的那些分析了。尽管它是难的由于已知的海洋的免费生活的线虫的18SrDNA顺序数据的缺乏把这些序列分到已知的种类或类，获得的序列被分到Adenophorea的线虫。在他们之中，12个序列接近了PontonemavulgareandAdoncholaimussp，四到Daptonemaprocerus并且二(相同)到Enoplusbrevis。我们的结果证明免费生活的海洋的线虫差异能被检索的PCR决定，18SrDNA的分析定序，仅当免费生活的海洋的线虫的18SrDNA序列被积累到某程度，一个18SrDNA序列能被分到种或一个类。

简介：在转基因的过程，外国基因能集成于在二个方向的主机染色体，它可以影响它的表达式。以便学习insertalorientation的效果，gfp记者基因在Synechococcussp的isiAB地点被插入。PCC7942冷淡的方向，和GFP表示层次和转基因的水藻的生长被比较。gfp基因能在每个方向表示，这被显示出，并且没有重要差别在在二recombinant水藻之间的海藻的生长和GFP表示层次上被检测。

简介：Saccharina是最重要的无热水设备生活水兵褐之一海藻的类。在这研究，我们分析了S的transcriptome。装饰用的梨树，它属于1000植物(OneKP)工程，由使用定序技术的下一代的高产量的DNA。未加工的数据的大约5.16GB被产生，并且有454bp的平均长度的65536脚手架与肥皂denovo汇编方法被装配。总共，19040unigenes被强风识别；25734脚手架被聚类进37基因本体论功能的组；6760脚手架被分类进25个轮牙范畴，以及被分到306条KEGG小径的2665脚手架。unigenes的多数比另外的cyanobacteria，海洋的硅藻，和植物包括棕色的水藻和硅藻展出了更多的类似到水藻。Saccharina装饰用的梨树有突出的能力积累象Br那样的卤素，我从海水经由halogenation处理。我们在S获得了42不同钒依赖者haloperoxidases(vHPO)。装饰用的梨树transcriptome数据，包括钒依赖者iodoperoxidase(vIPO)的5个片断和钒依赖者bromoperoxidase(vBPO)的37个片断。识别fulllengthS的复杂分析。装饰用的梨树vBPO1和S。装饰用的梨树vBPO2在在海洋的海藻的vBPOs和vIPOs之间的棕色的水藻和强壮的关系的种类之中揭示了vBPO的重要性。这研究将提高我们生物特征的理解和S的经济价值。装饰用的梨树种类。