学科分类
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44 个结果
  • 简介:为了推进,改进高地大米变化Huhan3Huhan7,种子样品为约15d二艘可重获飞船被送到外层空间并且分别地为75代被宣传。Phenotypic分析表明词法特点蛋白质谷物直链淀粉内容变化了。由联系基因简单顺序重复(SSR)插入删除(InDel)genomic变化描述标记显示变化模式是很复杂。大多数变化在简单顺序重复碎片发生在碎片3结束或5结束。反向抄写聚合酶链反应(RT-PCR)试金证明在SSR那些部分变化影响了他们基因表示,显示那基因联系了标记将有用孤立功能基因。为繁殖地调查也表明有高产量,高质量干旱忍耐更多线能通过太空繁殖被选择。结果显示太空mutagenesis为米饭繁殖导致了分子变化,以及生理、词法变化。

  • 标签: 分子变异 航天诱变 旱稻 简单重复序列 突变基因 应用
  • 简介:Themajorstoragesubstanceinriceendospermisstarch,whichaccountsfor80%ofdrymatterweight.Inthisstudy,ricemutantflo7,selectedfromtheprogenyofNipponbare’stissueculture,displayedflouryandopaqueendosperm.Comparedwithitscorrespondingwildtype(WT)Nipponbare,themutantflo7producedlonger,narrower,thinnerandlightergrains.Thelevelsofglucose,fructoseandsucroseinthemutantflo7endospermwerehigherthanthoseintheWTendosperm,whereastheproteincontentwasnotaffected.Withrespecttobothamylosecontentandgelconsistency,themutantflo7waslowerthanWT,butitsalkalivaluewashigher.Scanningelectronmicroscopicexaminationsshowedthattheendospermofthemutantflo7containedirregular,looselypackedandcompoundstarchgranules.Geneticanalysisindicatedthatthemutantphenotypewasdeterminedbyasinglerecessivenucleargene.Theflo7locuswasmappedtoaregiononthelongarmofchromosome12,withina95.1kbintervaldefinedbythemarkersC2-11andC5-15.Thereare13openreadingframesinthemappinginterval.Transcriptionprofilingofthedevelopinggrainsshowedthatanumberofgenesinvolvedinstarchsynthesiswereaffecteddifferentlyinthemutantflo7.

  • 标签: 胚乳突变体 基因突变 迹映射 水稻 表型 直链淀粉含量
  • 简介:布朗planthopper(Nilaparvatalugens圣?l)大多数损坏害虫之在米饭正在引起hopper灼伤,并且从而减少生产率并且另外产品质量。控制这个害虫有效管理策略是到本地米饭栽培变种理想基因鉴定转移。为开发抵抗栽培变种最重要途径是标记鉴定,它能在更持久抵抗遗传型帮助标记选择帮助。易受影响父母IR50抵抗父母Ptb33,和他们F2人口是为有随机放大多态DNA抵抗基因鉴定使用inbulkedsegregant分析标记(RAPD)教材。教材OPC7OPAG14证明主导、易受影响特定banding模式那么叫了co主导标记。而且,OPC7697OPAG14680给抵抗特定乐队看了并且因此在联合分阶段执行,而OPC7846OPAG14650给易受影响特定genotypic乐队看了inbulkedsegregant分析。因此,联合阶段标记,OPC7697OPAG14680,被认为在在庄稼改进米饭遗传型帮助标记选择更有用。

  • 标签: 水稻褐飞虱 抗性基因 识别标记 群分析 分子标记辅助选择 水稻基因型
  • 简介:到在米饭germplasm91-1A2米饭胆量小蚊抵抗被识别并且遗传上分析了。米饭人口F1s从作为父母米饭材料Jinggui,TN1,W1263(Gm1),IET2911(Gm2),BG404-1(gm3),OB677(Gm4),ARC5984(Gm5)Duokang1(Gm6)交叉91-1A2被导出。到米饭胆量小蚊所有父母线F1,BC1F1F2人口抵抗被识别。结果证明91-1A2所有F1s中国米饭胆量小蚊遗传因子型IV抵抗。到在BC1F1F2易受影响抵抗植物分离比率被X2测试1:39:7规则给予,建议到中国米饭胆量小蚊遗传因子型IV91-1A2抵抗被是新抵抗基因二主导基因控制,已知米饭胆量小蚊抵抗基因非突变而产生之遗传因子。

  • 标签: 水稻种质 遗传分析 稻瘿蚊 抗性鉴定 显性基因控制 F2群体
  • 简介:Smallubiquitin-likemodifier(SUMO)-conjugatingenzymesareinvolvedinpost-translationalregulatoryprocessesineukaryotes,includingtheconjugationofSUMOpeptidestoproteinsubstrate(SUMOylation).SUMOylationplaysanimportantroleinimprovingplanttolerancetoabioticstresssuchassalt,drought,heatandcold.Herein,wereportedtheisolationofOsSCE1(LOC_Os10g39120)geneencodingaSUMO-conjugatingenzymefromrice(Oryzasativacv.Nipponbare)anditsfunctionalvalidationinresponsetodroughtstress.TheE2enzyme,OsSCE1,isoneofthreekeyenzymesinvolvedintheconjugationofSUMOtoitstargetproteins.ActivatedSUMOistransferredtothecysteineofanE2enzymeandthentothetargetlysineresidueofthesubstrate,withorwithoutthehelpofanE3SUMOligase.ExpressionofOsSCE1wasstronglyinducedbypolyethyleneglycol6000(PEG6000)treatment,whichsuggestedOsSCE1maybeinvolvedinthedroughtstressresponse.OverexpressionofOsSCE1(OsSCE1-OX)inNipponbarereducedthetolerancetodroughtstress.Conversely,thedroughttolerancewasslightlyimprovedbytheknockdownofOsSCE1(OsSCE1-KD).TheseresultswerefurthersupportedbymeasurementofprolinecontentinOsSCE1-OXandOsSCE1-KDtransgeniclinesunderinduceddroughtstress,whichshowedOsSCE1-KDtransgeniclinesaccumulatedhigherprolinecontentthanthewildtype,whereasOsSCE1-OXlinehadlowerprolinecontentthanthewildtype.ThesefindingssuggestedOsSCE1mayplayaroleasanegativeregulatorinresponsetodroughtstressinrice.

  • 标签: Oryza SATIVA drought stress small ubiquitin-like
  • 简介:Cd忍耐米饭幼苗translocation上H2O2预告处理效果用在Cd忍耐不同二米饭栽培变种(N07-6N07-63)被学习。malondialdehyde(MDA)内容,减少谷胱甘肽(GSH),非蛋白质thiols(NPT),phytochelatins(PC)谷胱甘肽S-transferase(GST)活动在暴露于各种各样处理二栽培变种之间被比较。结果证明50mol/LCd暴露显著地禁止了米饭生长,提高了GSH,NPT,PCMDA生产,并且增加了GST活动,并且二栽培变种之间有重要差别。更多Cd被搬运进N07-6射击。H2O2预告处理由进步在根增加GSH,NPTPC内容,以及GST活动减轻了Cd毒性。在N07-63这些参数增加度比在N07-6那些高,建议N07-63忍耐比N07-6更显著地被提高。氢过氧化物把Cdtranslocation归结为米饭射击,但是不同地在根影响了Cd内容。从上述结果,在到在二栽培变种之间H2O2预告处理Cddetoxification反应有显著差别,这可以被推测。

  • 标签: 水稻品种 过氧化氢 预处理 CD 谷胱甘肽S 个人电脑
  • 简介:阳离子exchangers(CAX)属于广泛地在最后十在植物tonoplasts被调查了cation/Ca2+exchanger总科。最近,在植物涉及重金属累积忍耐CAX角色为植物救治食物安全被学习了。在这微型评论,我们在Ca2+信号transduction总结Ca2+/H+antiporter角色,维持离子动态平衡并且扣押重金属进液泡。而且,我们在场在重金属detoxification血浆膜Ca2+/H+antiporter个可能角色。

  • 标签: 转运蛋白 逆向 质膜 耐受性 重金属积累 钙信号转导
  • 简介:有低glutelin内容瑞斯作为为肾失败影响病人功能食物合适。在米饭低glutelin内容基因Lgc1有在二高度类似的glutelin基因GluB4GluB5之间3.5-kb删除,它在染色体2短手臂上定位。在低glutelin内容米饭改进选择效率繁殖,指定为InDel-Lgc1-1InDel-Lgc1-2二个分子标记被开发检测低glutelin内容基因Lgc1。双PCR察觉显示二个标记联合使用能容易把Lgc1遗传型不同米饭变化区分开来。作为种简单、便宜技术,因此,分子标记能广泛地被用来Lgc1基因识别不同变化并且在低glutelin内容米饭帮助标记选择适用。

  • 标签: 蛋白基因 水稻育种 分子标记 蛋白含量 ORYZA PCR检测
  • 简介:到怀有基因Pi-d2从pCB6.3kb,pCB5.3kbpZH01-2.72kb三不同表示向量转变了米饭强风抵抗转基因米饭线米饭强风抵抗被分析。有Pi-d2基因九根先进产生转基因米饭线显示了各种各样抵抗到39米饭强风紧张,并且最高疾病抵抗频率到达了91.7%。有Pi-d2基因四根早产生同型结合转基因线展出了抵抗到58米饭强风紧张中超过81.5%个,显示出宽光谱抵抗特征。当在文化媒介粗略毒素集中增加了,米饭强风真菌粗略毒素选择转基因胚胎calli证明从转基因米饭植物不成熟胚胎胼胝正式就职率减少了。当粗略毒素集中到达了40%时,从转基因线不成熟胚胎胼胝正式就职率是49.3%,并且受体控制是5%。在正式就职下面的地里转基因大米线颈强风疾病发生是0%~50%,显示转基因大米线大米强风抵抗比受体控制高得多。

  • 标签: 转基因水稻植株 水稻稻瘟病 抗性基因 Pi 幼胚愈伤组织 胚性愈伤组织
  • 简介:Tiller角度,个很必要农学特点,在米饭繁殖是重要,特别在植物类型繁殖。控制2个tiller角度(tac2)异种被乙醇甲烷磺酸盐mutagenesis从restorer线Jinhui10获得。tac2异种在幼苗阶段显著地在tillering阶段增加tiller角度显示了正常显型。初步生理研究显示异种GA敏感。因此,TAC2TAC1可能以方法控制tiller角度,这被推测。基因分析证明变异特点被主要后退基因控制并且位于用SSR标记染色体9。在TAC2和它最近标记RM3320RM201之间基因距离分别地是19.2厘米16.7厘米。

  • 标签: 分蘖角度 遗传分析 水稻育种 隐性基因 突变体 SSR标记
  • 简介:Rice(Oryzasativa)issensitivetosalinity,butthesalttoleranceleveldiffersamongcultivars,whichmightresultfromnaturalvariationsinthegenesthatareresponsibleforsalttolerance.High-affinitypotassiumtransporter(HKTs)hasbeenproventobeinvolvedinsalttoleranceinplants.Therefore,wescreenedfornaturalnucleotidepolymorphisminthecodingsequenceofOsHKT1,whichencodestheHKTproteinineightVietnamesericecultivarsdifferinginsalttolerancelevel.Intotal,sevennucleotidesubstitutionsincodingsequenceofOsHKT1werefound,includingtwonon-synonymousandfivesynonymoussubstitutions.Furtheranalysisrevealedthatthesetwonon-synonymousnucleotidesubstitutions(G50TandT1209A)causedchangesinaminoacids(Gly17ValandAsp403Glu)atsignalpeptideandtheloopofthesixthtransmembranedomain,respectively.Toassessthepotentialeffectofthesesubstitutionsontheproteinfunction,the3DstructureofHKTproteinvariantswasmodelledbyusingPHYRE2webserver.Theresultsshowedthatnodifferencewasobservedwhencomparedthosepredicted3DstructureofHKTproteinvariantswitheachother.Inaddition,thecodonbiasofsynonymoussubstitutionscannotclearlyshowcorrelationwithsalttolerancelevel.Itmightbeinterestingtofurtherinvestigatethefunctionalrolesofdetectednon-synonymoussubstitutionsasitmightcorrelatetosalttoleranceinrice.

  • 标签: 水稻科学 农业
  • 简介:ThecompleteopenreadingframeofOsPIN1awasamplifiedthroughreversetranscriptase-polymerasechainreaction(RT-PCR)basedonthesequencedepositedinGenBanktoexploretherelationshipbetweentheauxineffluxproteinOsPIN1aandthenegativephototropismofriceroots.SequencingresultsshowedthattheGCcontentofOsPIN1awas65.49%.ThefusionexpressionvectorpCAMBIA-1301-OsPIN1a::GFPcontainingtheOsPIN1ageneandacodinggreenfluorescentprotein(gfp)genewasconstructed.ThefusionvectorwastransferredintoonionepidermalcellsbyAgrobacteriumtumefacienstransformation.ThetransientexpressionofOsPIN1a-GFPwasmainlylocatedinthenucleusandcellmembrane.Moreover,thetransgenicplantswereobtainedbyAgrobacterium-mediatedgenetictransformation.MoleculardetectionperformedbyusingPCRandβ-glucuronidasestainingshowedthatthetargetconstructwasintegratedintothegenomeofrice.Thenegativephototropiccurvaturesofthetransgenicricerootswerehigherthanthoseofthewildtype.Similarly,theexpressionlevelsofOsPIN1ainthetransgenicplantswereconsiderablyhigherthanthoseinthewild-typeplants.TheseresultssuggestthatOsPIN1aiscrucialinthenegativephototropiccurvatureofriceroots.

  • 标签: RICE OsPIN1a green FLUORESCENT protein TRANSIENT
  • 简介:Byusing304recombinantinbredlinesderivedfromindicaricecrossZhong156/Gumei2,alinkagemapconsistingof177markerlociandcovering12ricechromosomeswasconstructedandemployedformappinggenesconferringblastresistanceinrice.GenomiclocationofgenePi25(t)conferringneckblastresistancetotheChineseisolate92-183(raceZC15)wasverifiedtobelocatedbetweenmarkersA7andRG456onchromosome6,withgeneticdistancesof1.7cMand1.5cMtoA7andRG456,respectively.LeafblastresistanceofGumei2tothePhilippineisolateCa89(lineage4)wasfoundtobecontrolledbyasinglegene.ThegenetentativelydesignatedasPi26(t)waslocatedbetweenmakersB10andR674onchromosome6,withgeneticdistancesof5.7cMand25.8cMtoB10andR674respectively.ResistantallelesatbothgenelociwerederivedfromGumei2,indicatinganexistenceofresistancegeneclusterinGumei2.

  • 标签: 水稻 稻瘟病 抗病性 分子标记 基因簇 Gumei
  • 简介:Membersoftheactivityofbc1complex(ABC1)familyareproteinkinasesthatarewidelyfoundinprokaryotesandeukaryotes.PreviousstudiesshowedthatseveralplantABC1genesparticipatedintheabioticstressresponse.Here,wepresentthesystematicidentificationofriceandArabidopsisABC1genesandtheexpressionanalysisofriceABC1genes.Atotalof15and17ABC1genesfromthericeandArabidopsisgenomes,respectively,wereidentifiedusingabioinformaticsapproach.Phylogeneticanalyseso...

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  • 简介:米饭镉(Cd)敏感变异cadB-1用Agrobacteriumtumefaciens被获得调停系统。在cadB-1野类型(WT)暴露以后米饭幼苗到为有增加外部Cd集中cadB-1WT10d,在根积累到高水平Cd,茎叶子Cd集中个范围,并且在cadB-1幼苗生长抑制比在WT更严肃。氢过氧化物累积在cadB-1叶子根是更高。减少谷胱甘肽(GSH)比率/oxidized谷胱甘肽(GSSG),ascorbate(ASC)/dehydroascorbate(DHA)减少菸碱腺嘌dinucleotide磷酸盐(NADPH)/oxidized菸碱腺嘌dinucleotide磷酸盐(NADP+)在高Cd层次下面在叶子根两个都比在WT在cadB-1是更低。ascorbateperoxidase(APX)活动,谷胱甘肽peroxidase(GR),dehydroascorbatereductase(DHAR)monodehydroascorbatereductase(MDHAR)在Cd高水平处理下面在叶子根两个都比在WT在cadB-1是也更低。我们结果建议在Cd应力下面,ASC-GSH周期更严重比在WT在cadB-1被禁止,显示变异cadB-1不太能清除反应氧种类并且Cd敏感。

  • 标签: 脱氢抗坏血酸还原酶 还原型谷胱甘肽 镉(CD) 水稻幼苗 突变体 还原型烟酰胺腺嘌呤二核苷酸