简介:摘要:目的 建立UPLC-MS/MS法同时测定大败毒胶囊中的芍药苷、绿原酸、橙皮苷3个成分的含有量。方法 Agilent poroshell 120 EC-C18(2.1mm×50mm,1.9μm)色谱柱,流动相A为乙腈,流动相B为0.02%甲酸水溶液,梯度洗脱,柱温为35℃,流速为0.2ml/min,进样量为5μl;电喷雾(ESI)离子源。结果 各组分在线性范围内线性关系良好,平均加样回收率98.1%-102.1%,RSD1.83%-4.42%。结论 该方法结果准确可靠,效率高,可为大败毒胶囊的质量控制研究提供科学依据。
简介:AbstractBackground:The human brain is the most complex organ in the body, and it is important to have a better understanding of how the protein composition in the brain regions contributes to the pathogenesis of associated neurological disorders.Methods:In this study, a comparative analysis of the frontal and temporal cortex proteomes was conducted by isobaric tags of relative and absolute quantification (iTRAQ) labeling and two-dimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS). Brain protein was taken from relatively normal tissue that could not be avoided of damage during emergent surgery of the TBI (traumatic brain injury) patients admitted in Beijing Tiantan Hospital from 2014 to 2017. Eight cases were included. Four frontal lobes and 4 temporal lobes proteome were analyzed and the proteins were quantitated. Gene Ontology (GO), Ingenuity Pathway Analysis (IPA), and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were used to analyze the biological function of identified proteins, unchanged proteins, and differentially expressed proteins (DEPs).Results:A total number of 2127 protein groups were identified in the frontal and temporal lobe proteomes. A total of 1709 proteins could be quantitated in both the frontal and temporal cortex. Among 90 DEPs, 14 proteins were screened highly expressed in the temporal cortex, including MAPT, SNCG, ATP5IF1, GAP43, HSPE1, STMN1, NDUFS6, LDHB, SNCB, NDUFA7, MRPS36, EPDR1, CISD1, and RALA. In addition, compared to proteins expressed in the frontal cortex, 14 proteins including EDC4, NIT2, VWF, ASTN1, TGM2, SSB, CLU, HBA1, STOM, CRP, LRG1, SAA2, S100A4, and VTN were a low expression in the temporal cortex. The biological process enrichment showed that unchanged proteins between the frontal and temporal cortex mainly take part in regulated exocytosis, axon guidance, and vesicle-mediated transport. The KEGG pathway analysis showed that unchanged proteins between the frontal and temporal cortex mainly take part in oxidative phosphorylation, carbon metabolism, Huntington's disease, and Parkinson's disease.Conclusions:The majority of proteins are unchanged between the frontal and temporal cortex, and unchanged proteins are closely related to its function. Among DEPs, MATP (tau) is upregulated in the temporal cortex, closely related to Alzheimer's disease (AD), and is one of the targets for the treatment of AD. CLU is downregulated in the temporal cortex which functions as an extracellular chaperone that prevents aggregation of non-native proteins. It was suggested that the temporal lobe may not be the "functional dumb area" of the traditional view, but could be involved in important neural metabolic circuits.
简介:摘要: 目的 采用UHPLC-MS/MS法测定中成药中西地那非、伐地那非、他达那非。方法 采用Agilent C18 (1.8μm,2.1×50mm) 色谱柱;流速:0.2mL/min ;流动相: A为含0.1%乙酸的0.02mol/L乙酸铵溶液,B相为甲醇,梯度洗脱;柱温35℃。结果 三种物质在所建立的色谱条件下均能较好分离, 在 20ng/mL~200ng/mL浓度范围内线性关系良好,相关系数均大于0.995,平均回收率97.3%~102.0%。结论 该方法前处理简单、选择性强,灵敏度高,可用于测定中成药中3种非法添加的壮阳类化学药物。
简介:摘要:建立了一种全新的以QuEChERS法为样品前处理技术结合UPLC-TQS法快速测定动物源性食品多种兽药残留的方法。样品经甲醇-乙腈混合溶液提取并通过DISQUETM净化管净化,上清液经正己烷除去脂肪、样品浓缩后用色谱柱进行分离,以甲醇5mmol/L醋酸铵含0.1%甲酸水溶液为流动相进行梯度洗脱,多反应监测(MRM)模式检测,外标法定量。结果表明:目标物在1~50μg/kg的空白添加浓度范围内均有良好线性关系,相关系数r≥0.99;样品中多种药物的检出限(LOD)均为0.5μg/kg,定量限(LQD)均为1.0μg/kg;在5~20μg/kg的添加水平范围内平均回收率为75.8%~93.2%,(RSD)为3.1%~13.2%。该方法具备快速、简便、准确性高、用性强等特点,适合动物源性食品中多类药物残留的定性定量检测。
简介:摘要:本文建立了一种利用液相色谱-串联质谱法LC-Ms/Ms测定蜂产品中氯霉素残留量的方法。样本提取、净化后,经乙腈-水流动相体系洗脱,C18色谱柱分析后,利用三重四级杆液相色谱质谱联用仪灵敏度高、专属性强、定量准确来检测氯霉素,可以用来快速筛查蜂蜜和蜂王浆中的氯霉素残留。
简介:摘要目的总结Spitz样肿瘤的临床及组织病理特征。方法回顾2005年1月至2020年1月西京皮肤医院确诊的320例Spitz样肿瘤患者的临床及病理资料。结果320例患者中,男141例,女179例,年龄0 ~ 65(12.5 ± 11.7)岁,病程1个月至30年;其中,Spitz痣307例,不典型Spitz肿瘤(AST)8例,Spitz痣样黑素瘤(SM)5例。皮损多为单发,可见于头面部、躯干和四肢,边界均清楚。307例Spitz痣皮损以黑色(132例,43.0%)和红色(108例,35.1%)为主,多数色素均匀(262例,85.3%)且表面平滑(272例,88.6%)。Spitz痣存在特殊临床亚型,11例(3.6%)发生在斑痣上,11例(3.6%)呈簇发性,6例(2.0%)播散性,7例(2.3%)结节性,1例(0.3%)为瘢痕疙瘩样。Spitz痣特征性病理表现包括表皮内痣细胞呈Paget样扩散(123例,40.1%),真表皮交界处出现Kamino小体(74例,24.1%),痣细胞呈水平带状(177例,57.8%)及楔形分布(118例,38.4%),痣细胞巢周围出现裂隙(177例,57.8%),可见生理性核分裂象(117例,38.1%),核染色质均细腻。根据特殊组织病理表现,Spitz痣又分为色素性上皮样Spitz痣(9例,2.9%)、结缔组织增生性Spitz痣(13例,4.2%)、血管瘤样Spitz痣(8例,2.6%)、疣状Spitz痣(12例,3.9%)、黏液样Spitz痣(10例,3.3%)、晕痣样Spitz痣(4例,1.3%)等。4例AST皮损为黑色,7例色素均匀,3例皮损表面粗糙;特征病理表现包括细胞均有轻度至中度的异型性,均可见核分裂象(7例为2 ~ 6个/mm2),5例核染色质粗糙。3例SM皮损呈红色,4例色素不均匀,3例表面粗糙;特征病理表现包括黑素细胞呈Paget样扩散(3例),瘤细胞呈无极性浸润性生长且均未见成熟现象,均有明显异型性,并可见病理性核分裂象(3例,> 6个/mm2),核染色质均粗糙且核膜明显着色。结论Spitz样肿瘤的临床及组织病理表现具有特征性,Spitz痣的临床及病理亚型繁多,AST同时具有Spitz痣和黑素瘤的临床及组织学特征。
简介:摘要: 飞机风挡加温功能与飞行安全密切相关,风挡温度的准确采集直接关乎风挡加温功能的正常工作。TMS320F28377片内包含丰富的AD转换资源,利用片内AD实现了多路风挡温度传感器的高速采集。该设计与传统的利用外置AD的设计方法相比,简化了电路设计,提高了电路的基本可靠性。经工程实践证明,该设计采集精确,工作稳定可靠。