简介：AbstractBackground:Rheumatoid arthritis (RA), a systemic autoimmune disease characterized by synovial inflammation, can cause cartilage and bone damage as well as disability. The aim of this study was to explore whether serum glucose-6-phosphate isomerase (GPI) is correlated with disease activity and the value of GPI in the evaluation of infliximab treatment in patients with RA.Methods:Sixty-two patients with RA who had an inadequate response to methotrexate (MTX) were enrolled in Peking University People’s Hospital from July 1, 2016 to July 31, 2018. Infliximab (3 mg/kg, intravenous at weeks 0, 2, and 6 and then every 8 weeks) was administered to patients with stable background MTX therapy. Serum samples were obtained at baseline and week 18. Serum GPI levels were determined using enzyme-linked immunosorbent assay. The associations between serum GPI levels and clinical features were analyzed.Results:Serum GPI was positively correlated with Disease Activity Score in 28 joints (DAS28), swollen joint count, tender joint count and C-reactive protein level (P < 0.001, P < 0.001, P < 0.001, and P = 0.033, respectively). The change of DAS28 in GPI-positive patients was greater than that in GPI-negative patients (P < 0.001). Compared with those for patients receiving MTX monotherapy at baseline, the GPI levels were significantly declined when MTX was combined with infliximab (P < 0.001).Conclusion:Serum GPI is related to disease activity and clinical response to infliximab treatment.

简介：抽象Trehalose在保护有机体免受各种各样的压力的伤害起一个重要作用。Trehalose-6-phosphatesynthase(TPS)是在trehalose合成的关键酶，但是在昆虫，一些仅仅TPS基因被识别了，他们的功能很好没被描绘。更好在昆虫理解TPS的功能，完全的TPS互补DNA(cDNA)克隆从蝗虫草之小穗migratoriamanilensis的胖身体被获得(GenBank就职数字：EU131894)。全身的cDNA是2806bp，包括2442bp的一个开的读物框架，它与91976道尔顿的计算分子的重量和6.14的一个等电位的点编码813氨基酸蛋白质。推出的氨基酸顺序高度类似于另外的出版昆虫TPS，它的C终端也有对trehalose磷酸盐phsophatase(TPP)相应的一个区域。半量的分析显示TPS抄本不仅在胖身体，而且在内脏，血淋巴和腿肌肉被表示。这些数据可以在昆虫便于TPS功能的研究并且改进我们trehalose新陈代谢的理解。

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简介：螺旋体serovars引起的威胁生活的感染为设计反细螺旋体病药要求需要。现在的学习包含对螺旋体的phosphoheptoseisomerase(GmhA)探索禁止者，它为lipopolysaccharide(LPS)是重要的生合成并且通过减少性的genomic途径作为一个普通的药目标被识别。GmhA模型在Modeller9v7被造。预言的模型的结构的精炼和精力最小化用艺术大师9.0被执行。精制模型可靠性通过Procheck，ProSA，ProQ和侧面3D被估计。基于底层虚拟高产量的屏蔽(VHTS)在Ligand。信息元数据库工具产生了354底层的一个内部图书馆结构的类似物。而且，从有每ligand的不同符合构造的内部图书馆的基于结构的VHTS提供了14个新奇竞争禁止者。和从VHTS获得的卓见的模型将是为开发反细螺旋体病的一个有希望的起点竞争禁止者指向LPS生合成小径。

简介：AnovelphotoelectrochemicalbiosensorincorporatingnanosizedCdSsemiconductorcrystalswithenzymetoenhancephotochemicalreactionhasbeeninvestigated.CdSnanoparticlesweresynthe-sizedbyusingdendrimerPAMAMasinnertemplates.TheCdSnanoparticlesandglucoseoxidase(GOD)wereimmobilizedonPtelectrodevialayer-by-layer(LbL)techniquetofabricateabiological-inorganichybridsystem.Underultravioletlight,thephoto-effectoftheCdSnanoparticlesshowedenhancementofthebiosensortodetectglucose.PtnanoparticlesweremixedintotheNafionfilmtoimmobilizetheCdS/enzymecompositesandtoimprovethechargetransferofthehybrid.Experimentalresultsdemon-stratethedesirablecharacteristicsofthisbiosensingsystem,e.g.asensitivityof1.83A/(mMcm2),lowerdetectionlimit(1M),andacceptablereproducibilityandstability.

简介：Wehaveintroducedtheglousesupporter(GS)formicrosurgerysince1991andreporteditsexperimentalstudiesin1992,andnowwepresentitsresultsonclinicalapplicationsinthispaper.GSismadefromglucose,sodiumchlorideanddextranuminproperratio.Ithasbeenprovedtohavenomaterialtoxicityonanimalsandhuman

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简介：ＡＮＥＷＴＹＰＥＯＦＧＬＵＣＯＳＥＳＥＮＳＯＲＦＯＲＣＬＩＮＩＣＡＬＴＥＳＴＹｉｋａｉＺｈｏｕ，ＨｏｎｇＬｉ，ＳｈｕＲｅｎ（ＤｅｐａｒｔｍｅｎｔｏｆＥｎｖｉｒｏｎｍｅｎｔａｌＭｅｄｉｃｉｎｅＴｏｎｇｊｉＭｅｄｉｃａｌＵｎｉｖｅｒｓｉｔｙ，Ｗｕｈａｎ，...

简介：Viaseveraltechniquestomodifysensitivematrixforinhabitingtheleakageoffluorescentindicator,anewstablesensingmaterialformonitoringphosphateshasbeenpreparedandappliedtothemeasurementofphosphatesinartificialseawater.ItisbasedonthereactionofPO3-4withAl(Ⅲ)-Morinthatleadstothefluorescencequenchingofthecompositematrix.AtpHvalue4.0andthesalinityvalue25.0,theresponsetimeofoutputsignalhavingreachedthesteadystateislessthan300seconds.ItscalibrationgraphisgainedintherangeofH2PO-4massconcentrationfrom1.50to7.00μg/mLwiththelimitofdetection(3σ/K)0.02μg/mL.Whenitwasusedtomeasurephosphatesinartificialseawater,therecoveryrangedfrom96.78%to102.09%.Beingstoredunderthepropercondition,themembranesmaintainsensitivefor90-120days,andareabletobeusedfor50-80timeswithindicatorsupplement.

简介：Astudyonthetransportcharacteristicsofzincinlousoilwithphosphateatdifferentconcentrationswascarriedoutbythemethodofstepinput.Theeffectsofphosphateandtemperatureonzinctransportwerestudiedthroughanalysingthediffusion-dipsersioncoefficients(D)andtheretardationfactor(R)obtainedbytheprogramCXTFIT.TheresultsshowedthatDdecreasedandRincreasedwithincreasigconcentrationofphosphatesothativwasdifficultforzinctobreakthroughthesoilcolumn,andzincstoppedtobreakthroughthecolumnathightemperature.Oneorderequation,doubleconstantequationandtheElovichequationwereallsuitablefortheescriptionofzincdynamics.Effectsofphosphateandtemperatureonzinctransportwerefurtherconfirmedbytheanalysisonpseudo-thermodynamicparametersofzinctransport.

简介：Weinvestigatehigh-modulusdegradablematerialsintendedtoreplacemetalsinbiomedicalapplications.Thesearetypicallycompositescomprisingapolylactide(PLA)matrixreinforcedwithphosphateglassfibres,whichprovidereinforcementsimilartoE-glassbutareentirelydegradableinwatertoproduce,principally,calciumphosphate.Wehavemadecompositesusingavarietyoffibrearchitectures,fromnon-wovenrandommatstounidirectionalfibretapes.Flexuralpropertiesintheregionof30GPamodulusand350MPastrengthhavebeenachieved-directlycomparabletoquotedvaluesforhumancorticalbone.Incollaborationwithothergroupswehavebeguntoconsiderthedevelopmentoffoamedsystemswithstructuresmimickingcancellousboneandthishasshownsignificantpromise.Thefibresinthesefoamedstructuresprovideimprovedcreepresistanceandreinforcementoftheporewalls.Todatethematerialshaveexhibitedexcellentcellularresponsesinvitroandfurtherstudiesareduetoincludeconsiderationofthesurfacecharacterofthematerialsandtheinfluenceofthisoncellinteraction,bothwiththecompositesandtheglassfibresthemselves,whichshowpromiseasastandaloneporousscaffold.

简介：CunanoclusterswereelectrochemicallydepositedonthefilmofaNafion-solubilizedmulti-wallcarbonnanotubes(CNTs)modifiedglassycarbonelectrode(CNTs-GCE),whichfabricatedaCu-CNTscompositesensor(Cu-CNTs-GCE)todetectglucosewithnon-enzyme.Thelinearrangeis7.0×10-7to3.5×10-3mol/Lwithahighsensitivityof17.76μA/(mmolL),withalowdetectionlimit2.1×10-7mol/L,fastresponsetime(within5s),goodreproducibilityandstability.

简介：Thepropertiesofimmobilizedglucoseoxidase(GOD)bythecomplexesofdiethylaminoethylcellulose(DEAEC)withdifferentpolymers,suchaspolymethylacrylicacid(PMAA),polyacrylicacid(PAA),polystyrenesulfonicacid(PSSA),polyvinylaleohol(PVA),polyethyleneoxide(PEO)andstyrene-maleicacidcopolymer(PSMA)wereinvestigated.TheactivityofimmobilizedGODwasobviouslyinfluencedbythecomponentoftheDEAECcomplexes.TherelativeactivityoftheimmobilizedGODreachedtomaximumandover90%ofthenativeGOD.whentheDEAEC-PMAADEAEC-PAAcomplexeswereusedasacarrierwiththemolarratioofDEAECandpolyacidofaboutone.Michaelisconstants(Km)oftheimmobilizedenzymesofDEAEC-GOD-PMAAandDEAEC-GOD-PAAweredeterminedtobe1.25and1.00,respectively.Moreover,theimmobilizedGODhasagoodstoragestabilityandcycliclife.

简介：Anewtypeoffiberopticglucosebiosensorbasedonfluorescencequenchinghasbeendesignedanditspropertieshavebeenstudied.Glucosecanbeoxidizedbyoxygenwhenglucoseoxidaseareusedasthecatalyst,therefore,theconcentrationofglucosecanbemeasuredbydetectingtheconsumptionofoxygen.Forthedetectionofoxygenconcentration,theruthenium(Ⅱ)complex,Ru(bpy)3Cl2,wereusedasthefluorescenceindicatoranditsfluorescencelifetimeweredetectedbylock-intechnology.Thedetectingrangeofthesensoris50-500mg/dlanditsresponsetimeis30seconds,showingthatthiskindofsensorsispossibletobeusedinclinicaldiagnosisanddetection.

简介：ＩｎｔｒｏｄｕｃｔｉｏｎＩｎ１９６３，ＭｏｏｒＡ．Ｍ．［１］ｆｉｒｓｔｌｙｒｅｐｏｒｔｅｄｔｈｅｐｈｏｓｐｈａｔｅｅｆｅｃｔ，ｉ．ｅ．，ｉｎｔｈｅＵＶｉｒｒａｄｉａｔｉｏｎ（２５３．７ｎｍ）ｏｆａｑｕｅｏｕｓｓｏｌｕｔｉｏｎｓｏｆ４－ａｍｉｎｏ－２－...

简介：INTRODUCTIONBonebiomaterialshavebeenwelldevelopedinrecentyearsandfrequentlyusedclinicallyinorthopedic,traumaticandodontologicalsurgeries.Amongst,bioceramicsissimilartothemineralconstituentsofourskeletonandhighlyappraisedduetoitsexcellentcharacteristicsinbio-compatibility,mechanicalandbio-degradableproperties,andbone-inductivity.

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简介：Theceramicsettingbindersobtainedbymeansoftherawmixmechano-chemicalprocessing(mechanicaldispersionandchemicalinteractionprocessescombination),usedforbondingrefractoryconcretes,becomemoreavailablewithasubsequentincreasedinterestintheirpropertiesbecausetheydevelopinitialstrengthduringaheatupoftheunitswithin8-10h,incontrastto24hafterplacementforhighAl2O3cements(HAC).Thisstrengthdevelopmentenableshightemperatureprocessingunitstoberelinedwithminimumturnaroundtime,thusprovidingafavoredcost/performanceratio.Thepresentpaperidentifiesthepredominantphosphatephasesresponsiblefordevelopinggoodmechanical-strengthpropertiesofzirconiaandaluminaconcretesbondedwithmechano-chemicalbinders.Itsetsoutthecolloidandcrystallinephasesresultedfrominterphaseinteractionthatprovidethebasisforhighconcentratedbondingsuspensions(HCBS)technologyusefultotherefractoryindustry.

简介：INTRODUCTIONCalciumphosphatecements(CPC)overcomethepracticaldisadvantagesofblocksorgranuleslcanbehandledasapasteandsitinsitu.TheirstructureandcompositionclosetothatofHAPmakethembiocompatiblematerials.2Theconventionalcalciumphosphatecementhadsomeproblemssuchaslongsettingtime(30～60min)andlowcompressivestrength,etc.Inoursystem,anα-TCP/TTCPpowdermixturewasmixedwithwatercontainingcitricacidtocontrolthesettingtimeandcompressivestrength.Inthispaper,theeffectsofvariousconcentrationcitricacidsolutionsonthepropertiesofthecementarereported.

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