简介:Objective:Tounderstandwhetherverapamil(VER)resistancedevelopmentinthemultidrug-resistantcelllineanditsmechanism.Methods:K562/ADM/VERcellsublineresistanttoverapamilwasestablishedthroughagradualincreaseofVERconcentrationinthemedia.MTTmethodwasusedtoassayresistancetoVER,crossresistancetodipyriamole(DPM),cyclosporinA(CsA)inthecells,andHPLCandspectrofluorometertodetectintracellularaccumulationofVERorADMrespectively,aswellasS-Pimmunocytochemicaltechniquefordetectionofgenesexpression.Results:Itwereobservedthat7.9-foldincreaseinVERresistance,significantlyreducedintracellularaccumulationofVERorADMandalsodevelopacrossresistancetoDPMandCsAinK562/ADM/VERcells,comparedwithitsparentcell,K562/ADM.High-levelofp-glycoprotein(pgp),middle-levelofp53,p16,waspresentintwocelllineswithoutexpressionofGSTPI,C-myc,C-myc,C-fosandC-erbB-2.Bc1-2proteinexpressionwasfoundonlyinK562/ADMcells.Conclusion:K562/ADMcellswerecapableofbeinginducedtodevelopresistancetoVER.
简介:摘要目的分析恩施地区2005至2014年眼球摘除原因,并比较前5年与后5年原因的变化趋势.方法分析在2005-2009年(136例)与2010-2014年(213例)期间于我院行眼球摘除手术的349例患者的主要原因,比较前5年与后5年眼球摘除原因构成比的差异.结果分析比较眼外伤组、恶性肿瘤组及内眼手术组二者有显著性差异,眼外伤组及内眼手术组有明显降低趋势,恶性肿瘤组增高.结论眼外伤的预防与积极的救治是挽救眼球的有力措施.眼科医疗技术的提高降低了丧失眼球的风险.关键词恩施地区;眼球摘除;原因;回顾性研究