简介：AninvivofluorescencediscriminationtechniqueforphytoplanktonpopulationwasdevelopedbyusingWaveletpackettransform,clusteranalysisandnon-negativeleastsquares.Thetechniquewasusedtoanalyzewatersamplesfromdifferentsearegions.Forsimulativemixtures,whendominantspeciesaccountfor60%,70%,80%,90%atthedivisionlevel,thecorrectdiscriminationratios(CDRs)are83.0%,99.1%,99.7%and99.9%withtherelativecontentsof58.5%,68.4%,77.7%and86.3%,respectively;whenthealgaedominanceare60%,70%,80%,90%,100%atthegenuslevel,theCDRsare86.1%,94.9%,95.2%,96.8%and96.7%,respectively.Forlaboratorymixtures,theCDRsare88.1%and78.4%atthedivisionandgenuslevel,respectively.Forfieldsamples,theCDRswere91.7%and80%atthedivisionandgenuslevel,respectively(mesocosmexperiments),andtheCDRswere100%and66.7%atthedivisionandgenuslevel,respectively(JiaozhouBay).ThefluorometrictechniquewasusedtoestimatethephytoplanktoncommunitycompositionandrelativeabundanceofdifferentclassesfortheApril2010cruiseintheYellowSeawiththeresultsagreeingwiththoseinpublishedpapersbyotherauthors.

简介：TostudytheTaiwanStrait(TS),anunusualseaarea,thenumericalmodelinmarginalseasofChinaisusedtosimulateandanalyzethetidalwavemotioninthestrait.ThenumericalmodelingexperimentsreproducetheamphidromicsystemoftheM2tideinthesouthendoftheTaiwanstrait,andconsequentlyconfirmtheexistenceofthedegenerateamphidromicsystem.Onthisbasis,furtherdiscussionisconductedontheM2systemanditsformationmechanism.ItcanbeconcludedthatthetidalwavesoftheTSisconsistedoftheprogressingwavefromthenorthentranceandthedegenerateamphidromicsystemfromthesouthentrance,inwhichtheprogressingwavefromthenorthentrancedominatesthetidalwavemotioninthestrait.Exceptfortheconvergenteffectcausedbythelandformandboundary,thedegenerateamphidromicsystemproducedinthesouthofthestraitisanotherimportantfactorforthefollowingphenomena:thelargetidalrangeinthemiddleofthestrait,theconcentrativezoneofco-amplitudeandco-phaselineinthesouthofthestrait.ThedegenerateamphidromicsystemismainlyproducedbytheincidentPacificOceantidalwavefromtheLuzonstraitandtheactionbytheshorelineandlandform.Thepositionoftheamphidromicpointiscompelledtomovetowardsouthwestuntildegeneratingbythepowerfulprogressingwavefromthenorthentrance.

简介：随着卫星遥感技术的不断发展,高分辨率卫星影像逐渐应用到水深遥感反演领域.利用Worldview-2高分辨率卫星数据和电子海图数据,基于双波段比值法,反演获得实验区域20m以浅的水深.实验表明,Worldview-2等高分辨率多光谱卫星数据,具有一定反演浅水水深的能力,但在5m以浅的水域反演误差较大;双波段比值法,这种半经验半理论的模型,在水深遥感反演中具有更好的适用性;对比了一次线性、二次多项式、指数、对数等拟合方法,发现对数拟合的方法获取绝对水深,其精度相对其他方法更高.

简介：Euphausiapacificisanimportantsourceofnaturalastaxanthin.StudieswerecarriedouttoassesstheextractabilityofastaxanthinfromE.pacificusingsubcritical1,1,1,2-tetrafluoroethane(R134a).Toexaminetheeffectsofmultipleprocessvariablesontheextractionyield,astaxanthinwasextractedundervariousconditionsofpressure(30-150bar),temperature(303-343K),time(10-50min),flowrate(2-10gmin-1),moisturecontent(5.5%-63.61%),andparticlesize(0.25-0.109mm).Theresultsshowedthattheextractionyieldincreasedwithtemperature,pressure,timeandflowrate,butdecreasedwithmoisturecontentandparticlesize.Amaximumyieldof87.74%wasobtainedunderconditionsof100bar,333K,and30minwithaflowrateof6gmin-1andamoisturecontentof5.5%.Thesubstantialastaxanthinyieldobtainedunderlow-pressureconditionsdemonstratesthatsubcriticalR134aisagoodalternativetoCO2forextractionofastaxanthinfromE.pacific.

简介：OnthebasisofdatacollectedintheJiaozhouBayinJuneandJuly2003,theDICdistributioninseawaterisstudied,andanaverageair-seafluxofCO2isestimated.TheresultsshowthatthecontentofDICinsidethebayismarkedlyhigherthanoutsidethebayinJune,butthecontentofDICoutsidethebayismarkedlyhigherthaninsidethebayinJuly.ThetrendofDICdistributioninsidethebayissimilar,viz.thecontentisthemaximuminthenortheast,thendecreasesgraduallytowardthewest,andthecontentistheminimuminthewest.Thetotaltrendofverticaldistributionistoincreasegraduallyfromsurfacetobottom.ThischaracteristicofDICdistributionisdeterminedbyJiaozhouBayhydrologyandthereisacloserelationbetweenDICandparticulateN,P.AverageCO2fluxacrossthesourceforatmosphericCO2inJuneandJuly,andthetotalCO2fluxfromseawaterintoatmosphereisabout740tinJuneand969tinJuly.

简介：一个全球海洋碳周期模型基于海洋将军发行量模型流行音乐和改进biogeochemical模型，OCMIP-2被采用在历史上观察的大气的CO2集中和不同未来情形(叫的代表性的集中小径，或RCP)下面模仿碳周期过程。在这份报纸的RCP为第五份评价报告(AR5)在气候变化(IPCC)上跟随Intergovernmental面板的设计。模型结果证明海洋从空气吸收CO2，被吸收将在四RCP下面在第21世纪继续。由空至海的CO2流动在历史的时间期间增加了并且到达的网1.87Pg/a(由碳计算了)在2005；然而，它将在第21世纪到达山峰然后减少。海洋主要专心于CO2中间的纬度，并且在赤道区域释放CO2。然而，在南极围绕天极的水流(ACC)区域，海洋将在升起的CO2集中下面从来源改变到水池，包括RCP4.5，RCP6.0，和RCP8.5。在2100，人为的碳将被搬运到40摥漠敶?桴?湥楴敲礠慥?獩爠畯桧祬?呼??湡?桴?潲敬漠???獩琠敨猠慭汬獥t

简介：Asimplethree-dimensionaltidalmodelisusedtoexaminetheM2tidalcurrentdistributioninanortheasternpartoftheEastChinaSea,especiallytheverticalvariationofthecurrentintheregion.ComputedM2currentiscomparedwithobservationsavailableandfoundtobeingoodagreement.Mainfeaturesofthecalculatingmethodinthisstudyare:(1)Verticalvariationofthetidalcurrentistakenasafunetionofthedepth-meanvelocity:(2)themethodisapplicabletoavarietyoftheverticaleddyviscosities;(3)ithasafineverticalresolution,especiallyneartheseabootom.So,thismethodnotonlyenablesustogetasteadystatesolutioneasilybutalsodepictseffectsofthefrictionontheverticalvariationofthecurrentmuchbetter.

简介：Althoughtheannualglobalsea-airCO2fluxhasbeenestimatedextensivelywithvariouswind-dependent-kparameterizations,uncertaintystillexistsintheestimates.Thesea-state-dependent-kparameterizationisexpectedtoimprovetheuncertaintyexistingintheseestimates.Inthepresentstudy,theannualglobalsea-airCO2fluxisestimatedwiththesea-state-dependent-kparameterizationproposedbyWoolf(2005),usingNOAA/NCEPreanalysiswindspeedandhindcastwavedatafrom1998to2006,andanewestimate,-2.18GtCyear-1,isobtained,whichiscomparablewithpreviousestimateswithbiochemicalmethods.Itisinterestingtonotethattheaveragedvalueofpreviousestimateswithvariouswind-dependent-kparameterizationsisalmostidenticaltothatofpreviousestimateswithbiochemicalmethodsbyvariousauthors,andthatthenewestimateisquiteconsistentwiththeseaveragedestimates.

简介：Thetyphoon,asamaturetropicalcyclonethatdevelopsinthewesternpartoftheNorthPacificOceanwithhighwindspeedandheavyrainfall,isoneofthemostlethalandcostlyofnaturaldisastersforthedenselypopulatedcountriesofEastAsia.Itcanbeeasilydetectedbyspace-bornesensorsoperatedatmicrowave,visibleorinfraredbands(Liuetal.,2014).SyntheticApertureRadar(SAR)is

简介：景观地图landscapemap表示地表多种自然地理要素的空间分布和规律的综合地图.环境地图environmentalmap反映自然环境、人类活动对自然环境的影响和环境对人类的危害及环境治理等内容的地图.

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简介：β-N-乙酰-D-氨基葡萄糖苷酶与南美白对虾的食物消化吸收、蜕壳生长有着密切关系.海水里存在的有机污染物将影响酶生理功能,从而进一步影响虾的正常蜕壳,严重将导致对虾的死亡.甲醛是常用的有机溶剂,故本文应用动力学方法研究了南美白对虾β-N-乙酰-D-氨基葡萄糖营酶在甲醛溶液中以pNP-NAG为底物时酶活力的变化规律.表明酶在甲醛浓度低于2.0mol·L-1,酶的失活过程是可逆的,测得甲醛对酶抑制的IC50为1.05mol·L^-1.用双倒数作图法测定甲醛对酶的抑制类型,结果显示甲醛是酶的竞争性抑制剂,甲醛只能与游离酶（E）结合,底物对酶的失活具有保护作用.用底物反应动力学方法观测在不同底物浓度下酶在0.0、0.5、1.0、1.5、2.0mol·L^-1的醋酸酐溶液中的失活过程,分别测定了酶的微观失活速度常数k＋0及复活速度常数k-0并加以比较,结果表明甲醛对酶的影响是缓慢结合同时导致失活的过程,比较微观失活速度常数k＋0及复活速度常数k-0,结果暗示在高浓度的甲醛溶液中,酶将完全失活.

简介：β-N-乙酰-D-氨基葡萄糖苷酶与南美白对虾的食物消化吸收、蜕壳生长有着密切关系。海水里存在的有机污染物将影响酶生理功能,从而进一步影响虾的正常蜕壳,严重将导致对虾的死亡。甲醛是常用的有机溶剂,故本文应用动力学方法研究了南美白对虾β-N-乙酰-D-氨基葡萄糖苷酶在甲醛溶液中以pNP-NAG为底物时酶活力的变化规律。表明酶在甲醛浓度低于2.0mol?L-1,酶的失活过程是可逆的,测得甲醛对酶抑制的IC50为1.05mol?L-1。用双倒数作图法测定甲醛对酶的抑制类型,结果显示甲醛是酶的竞争性抑制剂,甲醛只能与游离酶(E)结合,底物对酶的失活具有保护作用。用底物反应动力学方法观测在不同底物浓度下酶在0.0、0.5、1.0、1.5、2.0mol?L-1的醋酸酐溶液中的失活过程,分别测定了酶的微观失活速度常数k+0及复活速度常数k-0并加以比较,结果表明甲醛对酶的影响是缓慢结合同时导致失活的过程,比较微观失活速度常数k+0及复活速度常数k-0,结果暗示在高浓度的甲醛溶液中,酶将完全失活。

简介：TheeffectsofBacillussubtilis2-1fromtheintestineofhealthyseacucumberonthegrowth,digestiveenzymeactivitiesandintestinalmicrobiotaofjuvenileseacucumber(Apostichopusjaponicus)weredeterminedinthepresentstudy.SeacucumberwasfedwithSargassumthunbergiipowdersupplementedwithB.subtilis2-1atdifferentconcentrationsvaryingamong0(control),105,107,and109CFUg-1for8weeks.ResultsshowedthatthegrowthperformanceandintestinalamylaseandtrypsinactivitiesweresignificantlyincreasedbydietaryB.subtilis2-1at109CFUg?1(P<0.05).However,dietaryB.subtilis2-1hadnosignificantinfluenceonthelipaseactivityinseacucumber(P>0.05).Thepolymerasechainreactiondenaturinggradientgelelectrophoresisand16SrRNAgenesequencinganalysisindicatedthatdietaryB.subtilis2-1at105and107CFUg-1inhibitedmostoftheProteobacteriaincludingthoseingenusVibrio.DietaryB.subtilis2-1at109CFUg-1notonlydecreasedtheabundanceandspeciesofgenusVibrio,butalsoincreasedtheintensityofgeneraPsychrobacterandBacillus.AspecificdosageofdietaryB.subtilis2-1couldincreasethegrowthandmodulatetheintestinalmicrobiotaofseacucumber;thusitmightbeanovelprobioticforkeepingthehealthofseacucumber.

简介：Newsatellite-derivedlatentandsensibleheatfluxesareperformedbyusingWindSatwindspeed,WindSatseasurfacetemperature,theEuropeanCentreforMedium-rangeWeatherForecasting(ECMWF)airhumidity,andECMWFairtemperaturefrom2004to2014.The55mooredbuoysareusedtovalidatethembyusingthe30minand25kmcollocationwindow.Furthermore,theobjectivelyanalyzedair-seaheatfluxes(OAFlux)productsandtheNationalCentersforEnvironmentalPrediction-NationalCenterforAtmosphericResearchreanalysis2(NCEP2)productsarealsousedforglobalcomparisons.ThemeanbiasesofsensibleandlatentheatfluxesbetweenWindSatfluxresultsandbuoyfluxdataare–0.39and–8.09W/m~2,respectively.Inaddition,therootmean-square(RMS)errorsofthesensibleandlatentheatfluxesbetweenthemare5.53and24.69W/m~2,respectively.TheRMSerrorsofsensibleandlatentheatfluxesareobservedtograduallyincreasewithanincreasingbuoywindspeed.Thedifferenceshowsdifferentcharacteristicswithanincreasingseasurfacetemperature,airhumidity,andairtemperature.ThezonalaveragelatentfluxeshavesomehighregionswhicharemainlylocatedinthetradewindzoneswherestrongwindscarrydryairinJanuary,andthemaximumvaluecentersarefoundintheeasternwatersofJapanandontheUSeastcoast.Overall,theseasonalvariabilityispronouncedintheIndianOcean,thePacificOcean,andtheAtlanticOcean.Thethreesensibleandlatentheatfluxeshavesimilarlatitudinaldependencies;however,somedifferencesarefoundinsomelocalregions.

简介：Intertidalmarinemacroalgaeexperienceperiodicalexposuresduringlowtideduetotheirzonationaldistribution.ThedurationofsuchemersionleadstodifferentexposuresoftheplantstolightandaerialCO2,whichthenaffectthephysiologyofthemtodifferentextents.TheecophysiologicalresponsestolightandCO2wereinvestigatedduringemersionintworedalgaeGloiopeltisfurcataandGigartinaintermedia,andtwobrownalgaePetaloniafasciaandSargassumhemiphyllum,growingalongtheShantoucoastofChina.Thelight-saturatednetphotosynthesisinG.furcataandP.fasciashowedanincreasefollowedbyslightlydesiccation,whereasthatinG.intermediaandS.hemiphyllumexhibitedacontinuousdecreasewithwaterloss.Inaddition,theupper-zonatedG.furcataandP.fascia,exhibitedhigherphotosynthetictolerancetodesiccationandrequiredhigherlightleveltosaturatetheirphotosynthesisthanthelower-zonatedG.intermediaandS.hemiphyllum.Desiccationhadlesseffectondarkrespirationinthesefouralgaecomparedwithphotosynthesis.Thelight-saturatednetphotosynthesisincreasedwithincreasedCO2concentrations,beingsaturatedatCO2concentrationshigherthanthepresentatmosphericlevelinG.furcata,G.intermediaandS.hemiphyllumduringemersion.ItwasevidentthattherelativeenhancementofphotosynthesisbyelevatedCO2inthosethreealgaeincreased,thoughtheabsolutevaluesofphotosyntheticenhancementowingtoCO2increasewerereducedwhenthedesiccationstatusesbecamemoresevere.However,inthecaseofdesiccatedP.fascia(waterlossbeinggreaterthan20%),lightsaturatednetphotosynthesiswassaturatedwithcurrentambientatmosphericCO2level.ItisproposedthatincreasingatmosphericCO2willenhancethedailyphotosyntheticproductioninintertidalmacroalgaebyvariedextentsthatwererelatedtothespeciesandzonation.

简介：《海洋环境保护法》第89条第2款本身并非关于诉讼的直接规定,该款规定的内容也不符合民事公益诉讼的内涵特征,将该款解释为关于海洋环境民事公益诉讼的规定不利于我国立法内部的横向协调。我国司法实践总体上也不认可《海洋环境保护法》第89条第2款规定的内容属于海洋环境民事公益诉讼。《海洋环境保护法》第89条第2款应是关于损害赔偿请求权的特别规定,不是我国海洋环境民事公益诉讼的法律依据,相应的法律依据应是《环境保护法》第58条、《民事诉讼法》第55条以及相关司法解释。