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简介：【摘要】目的：分析在产前胎儿脐带绕颈诊断中运用B超检查的应用价值。方法：此次研究筛选2019年12月-2020年12月期间我医院接收的产前检查孕妇40例作为本文中的分析对象，对所有孕妇采取产前B超检查，了解掌握胎儿是否存在脐带绕颈情况，并将检查结果与最终分娩结果进行详细对比。结果：最终对比结果显示,40例产妇产前应用B超检查结果中有10例胎儿存在脐带绕颈，其中包括脐带绕颈1周4例，绕颈2周6例；而产后结果显示40例产妇中胎儿存在脐带绕颈有11例，其中绕颈1周5例，绕颈2周6例，最终B超结果与产后结果比较符合，数据结果对比无统计学意义（P＞0.05）。结论：对产妇及时开展产前B超检查能够有效掌握胎儿脐带绕颈存在情况，提高产妇妊娠安全性。

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简介：ToinvestigatetheHLA-A,-BallelepolymorphisminHanpopulationofShandongprovinceandtoexplorethepossibilitytofindouttheHLA-A,-B-matchedcordblooddonorsforstemcelltransplantationtobeusedinotherareainChina,5844umbilicalcordbloodsamplesweretakenfromHanpopulationdonorsofShandongprovince,andassayedwithPCR-sequence-oligonucleotide(PCR-SSO)assay.InShandongHandonors,20allelesatHLA-Alocusand46allelesatHLA-Blocuscouldbedetectedasrevealedinthepresentstudy.Amongthe20allelesatHLA-Alocus,themostprevalentfiveallelesincludedA*02(0.3041),A*11(0.1443),A*24(0.1434),A*30(0.0975)andA*33(0.0859),while,thealleleswithlowergenefrequenciesincludedA*34(0.0006),A*25(0.0005),A*66(0.0005),A*74(0.0004)andA*(0.0001).Ofthe46HLA-Ballelesdetected,themostprevalentfivealleleswereB*13(0.1348),B*51(0.0713),B*62(0.0712),B*61(0.0676)andB*60(0.0642);whilealleleswithlowergenefrequenciesincludedB*77(0.0001),B*76(0.0002),B*47(0.0003),B*42(0.0003)andB*72(0.0004).IncomparisonwiththoseoftheotherHanpopulationinChina,theHLA-A,-BgenefrequenciesintheumbilicalcordbloodofShandongprovincepossessuniquedistributionfeaturesamongtheinvestigatedpopulationsfromvariousregionsofthesameraceorigin,andthedifferencesinvariousregionsofthesameracewerelessthanthoseamongthedifferentrace.ItisevidentthattheHLA-A,-BallelesoftheumbilicalcordbloodtakeninShangdongprovinceshowhighdegreeofpolymorphism,anditmightbepartofthoseofNorthernHanpopulationinChina.So,itisreasonableforpatientsofNorthernChinesetoreceiveHLAclassI-matchtransplantofcordbloodstemcellsfortissueandorgantransplantationfromShangdongumbilicalcordbloodbank.

简介：ThepurposeofthisstudywastoconstructaneukaryoticDNAvectorencodingamultipleepitopeantigen(MFC)ofhepatitisCvirus(HCV)andahepatitisBsurfaceantigen(HBsAg),andexploretheeffectofHBsAggeneontheimmunityofHCVmultiple-epitopeDNAconstructinvitroandinvivoinmice.AnHCVDNAvector(pVAX1-HBs-MFC)wasconstructedbyfusingHBsAggenetotheNterminalofanHCVmultiple-epitopeantigengene.ThepVAX1-HBs-MFCwastransfectedintoHEK293TcellsanditsexpressionwasmeasuredbyELISAandWesternblotting.BALB/cmicewereintramuscularlyimmunizedwiththepVAX1-HBs-MFC,andanELISAapproachwasappliedtodeterminethespecificantibodytitersandsubtypesinthemouseserum.Thecross-reactivityoftheantibodieswasalsocheckedwithtwosynthesizedHCVhypervariableregion1(HVR1)peptides.TheIFN-γproductionandcellproliferationofthemousespleencellswereevaluatedbyELISAandMTS(3-[4,5-dimethylthiazol-2-yl]-5-[3-carboxymethoxyphenyl]-2-[4-sulfophenyl]-2H-tetrazolium,innersalt)assays,respectively.TheexpressionofpVAX1-HBs-MFCwasdetectableinthetransfectedHEK293Tcells.TheserumantibodyresponsewaseffectivelyelicitedinBALB/cmiceinjectedwithpVAX1-HBs-MFC.ThehighesttiterofantibodyagainstHCV(MFC)was1:1280,andtheratioofIgG2a/IgG1was1.50±0.12atthefifthweekafterfirstimmunization.Moreover,thecollectedmouseserumantibodyhadtheabilitytocross-reactwiththetwosynthesizedHCVHVR1peptides.ThestimulationindexofthemousesplenocytestoMFCwas1.79±0.07,andtheIFN-γlevelwas287±6pg/mlatweek21afterfirstimmunization.ThehighesttiteroftheantibodyincontrolBALB/cmiceimmunizedwithpVAX1-MFCwas1:320,andtheratioofIgG2a/IgG1was1.33±0.11atweek5post-immunization.Furthermore,thestimulationindexofthemousesplenocytescellstoMFCwas1.52+0.06,andtheIFN-γlevelwas225±9.3pg/mlatweek21post-immunization.TheHBsAggenecanenhancetheeffectsofanHCVmultiple-epitope

简介：【摘要】目的:研究并分析B超检查在腹部实质性脏器损伤诊断与治疗中的应用。方法：选取72例本院于2019年4月-2020年2月收治的腹部实质性脏器损坏患者，对上述患者均实施B超检查，并将手术病理诊断作为金标准，最终计算出B超检查的准确率。结果:经B超检查后可知，72例患者中B超检查中肾破裂（含左右肾脏），脾破裂、胰腺破裂、肝破裂的准确率分别为100.0%、92.9%、88.9%、94.1%，总准确率为97.22%。结论:通过对腹部实质性脏器损伤患者进行B超检查，具有较高的检出率，可应用于治疗前诊断与配合术中治疗，以提高患者的手术质量。

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简介：摘要：目的：了解3～6岁入托幼儿营养状况，膳食选择与饮食习惯的现状，为儿童科学合理的饮食和制定干预对策提供科学依据。方法：采用问卷调查辅以现场观察的方式获取我市入托幼儿的营养状况、膳食选择、膳食习惯等相关信息。结果：我市入托幼儿营养状况存在营养不良状况，超重肥胖率较高，营养不良性别上存在显著性差异(P

简介：【摘要】目的：分析B超在胎儿先天性心脏病初步筛查中的诊断价值。方法：选择2017年8月-2018年7月我院接受的200例孕妇作为案例，实施B超诊断，对结果分析。结果：在本次研究中对所有案例实施B超方式诊断，诊断出2例患者是先天性心脏病，确诊是先天性心脏病后，将其转到贵阳治疗。结论：B超在胎儿先天性心脏病初步检查中有重要的作用，整体检出几率高，诊断符合率高，对于简单的心脏病可以单独实施B超方式，可行性高。

简介：Toexploretheroleofnuclearfactor-κB(NF-κB)inthesignalpathwayofproteinkinaseC(PKC)regulatingtheproliferationandapoptosisofTlymphocytesinasthma.Tlymphocyteswereisolatedfromtheasthmaticmodelofguineapigsandtheasthmaticpatients.EithertheTcellsstimulatedwithPMAaloneorthosestimulatedwithPMAtogetherwithpyrrolidinedithiocarbamate(PDTC)wereincubatedfor1and24h.TheproliferationofandthepresenceofNF-κBinthecellsincubatedfor1hwereobservedbyMTTandimmunohistochemicalstaining,respectivelyAndthecellsincubatedfor24hwereobservedfortheapoptosisbyTUNEL.Alltheassayswereparalleledwithcontrols,andallthedatawereanalyzedstatisticallywiththesoftwareSAS.ThepercentageofcellsofnuclearpositivestainingofNF-scBandtheproliferationofTlymphocytesfromasthmaticguineapigsandasthmaticpatientsstimulatedwithPMAweresignificantlyhigherthanthoseofTlymphocytesfromasthmaticguineapigsandasthmaticpatientsstimulatedwithoutPMArespectively(P<0.01)andthoseofTlymphocytesfromnormalcontrolguineapigsandnormalcontrolpersonsstimulatedwithPMArespectively(P<0.01),andweresignificantlyreducedbyPDTC(P<0.01).TheapoptosisindexofTlymphocytesfromasthmaticguineapigsandasthmaticpatientsstimulatedwithPMAweresignificantlylowerthanthoseofTlymphocytesfromasthmaticguineapigsandasthmaticpatientsstimulatedwithoutPMArespectively(P<0.01)andthoseofTlymphocytesfromnormalcontrolguineapigsandnormalcontrolpersonsstimulatedwithPMArespectively(P<0.01),andweresignificantlyinducedbyPDTC(P<0.01).ThereweregoodpositivecorrelationbetweenthepercentageofcellsofnuclearstainingofNF-κBofTlymphocytesandtheproliferationofTlymphocytes(r=0.51-0.72,P<0.001),andalsogoodnegativecorrelationbetweenthepercentageofcellsofnuclearstainingofNF-scBandtheapoptosisindexofTlymphocytes(r=-0.55-0.71,P