简介:目的探讨骨髓象检验在急性白血病患者临床诊断中的应用情况。方法收集2013年—2016年治疗的120例急性白血病患者资料为观察组,均行骨髓象检验;另取120例同期健康体检者资料为对照组,比较两组检验结果和不同分型血象情况,计量资料组间比较采用检验,P〈0.05为差异有统计意义。结果观察组白血病患者LIM抗癌结构蛋白1指标明显较对照组低,结缔组织生长因子、血管内皮生长因子-C、抗凋亡基因水平则较对照组高(χ~2=70.584、85.129、71.698、38.457,均P〈0.05);M_1型血红蛋白水平较M_2、M_3型高(t=3.693、9.442,均P〈0.05),且M_2型又较M_3型高(t=4.385,P〈0.05);M_1、M_2型白细胞、血小板水平较M_3型高(t=3.251、2.830,均P〈0.05),且M_1型血小板水平较M_2型低(t=6.301,P〈0.05)。急性淋巴系白血病(acutelymphoblasticleukemia,ALL)血象分型中,L1型血红蛋白、白细胞水平较L2型高,血小板指标则较L2型低(χ~2=2.103、3.714、2.293,均P〈0.05)。结论骨髓象检验能够明确诊断白血病患者骨髓细胞及分型血象具体情况,为患者治疗提供有效参考数据。
简介:【摘要】在医学的不断发展中,对中医学进行有效的传承也是中医学发展和创新的基础,在中医学教学中,中医基础理论的教学是最为重要的部分,在中医基础理论教学中需培养学生的象思维,同时针对培养学生象维方式应进行相关研究。研究采用了对分课堂,在中医基础理论教学中培养学生象思维进行相关研究,旨在更好的培养中医基础理论教学中学生的象思维,能更好的为中医的传承提供教学理论的支持。
简介:摘要:目的:分析意象对话技术应用于非全身麻醉手术患者心理护理中的效果 。方法:选取 2018 年 6 月 ~2019 年 4 月 在 我院择期接受手术治疗的 78 例 患者作为观察对象 ,随机分为 A 组和 B 组,每组 39 例, 对两组均进行常规心理护理,对 A 组的常规心理护理应用 意象对话技术, 比较两组的护理前后 焦虑情绪量表( SAS )评分、 抑郁情绪量表( SDS )评分、血压及心率。结果: 在 SAS 评分和 SDS 评分方面,护理前两组无统计学差异 ( P > 0.05 )。两组护理后的各项评分均低于护理前( P < 0.05 )。 A 组护理后的各项评分均低于 B 组 P < 0.05 )。在 血压、心率方面,护理前两组无统计学差异 ( P > 0.05 )。两组护理后的各项数据均低于护理前( P < 0.05 )。 A 组护理后的各项数据均低于 B 组( P < 0.05 )。 结论:在对非全身麻醉手术患者的心理护理中应用 意象对话技术,可平复患者情绪,调整患者状态,提升治疗效果,对患者的手术治疗具有巨大价值。
简介:目的评价DNA分析在痰液涂片细胞学检查中作用和意义。方法对HE染色痰涂片褪色后行Feulgen染色,用HPIAS-1000型高清晰度彩色全自动分析系统作DNA分析。结果35例肺癌细胞及9例不典型支气管上皮细胞DNA均为异倍体(heteroploid,H)核型。两组细胞DNA二倍体,异倍体(2C/H)核型分别占37.1%和66.6%(P〈0.001),五倍体/非整倍体(5C/AN)核型分别占71.4%、0.0%(P〈0.001),非整倍体(AN)核型占97.1%、0.0%(P〈0.001);而增生期百分比(PF)分别占(83.8±19.7)%、(47.629±.5)%(P〈0.001)。癌细胞组29例非小细胞癌与6例小细胞癌的2C/H核型分别占44.8%和0.0%(P〈0.001),5C/AN占65.5%、100.0%(P〈0.001),非整倍体(AN)核型为96.5%、100.0%(P〉0.05);而PF值为(83.1±20.1)%、(95.0±2.0)%(P〈0.02)。结论痰液细胞DNA分析指标(倍体核型、增殖期百分比)可作为细胞学肺癌诊断的重要理论参考。
简介:Thedamageofhumancornealcellsencounterwiththeproblemofavailabilityofcornealcellsforreplacement.Limitationofthesourceofcornealcellshasbeenrealized.Anattemptofdevelopmentofcornealepithelial-likecellsfromthehumanskin-derivedprecursor(hSKPs)hasbeenmadeinthisstudy.Combinationofthreeessentialgrowthfactors:epidermalgrowthfactor(EGF),keratinocytegrowthfactor(KGF)andhepatocytegrowthfactor(HGF)coulddemonstratesuccessfullyinductionofhSKPstodifferentiationintocornealcells.Theinducedcellsexpressedtheappearanceofmarkersofcornealepithelialcellsasshownbythepresenceofkeratin3(K3)byantibodylabelandWesternblotassay.TheK3geneexpressionofinducedhSKPscellsasshownbyreversetranscription-polymerasechainreaction(RT-PCR)technologywasalsodemonstrated.ThepresenceofthesemarkersatbothgeneandproteinlevelscouldleadtoourconclusionthatthedirectionaltransdifferentiationofhSKPscellsintocornealepithelialcellswassuccessfullydoneunderthiscellinductionprotocol.Thefindingshowsanewlyavailablestemcellsourcecanbeobtainedfromeasilyavailableskin.Cellsfromautologoushumanskinmightbeusedforcornealdisordertreatmentinfutureclinicalapplication.
简介:〖摘要〗:易能垂象针法--紫白飞宫针理论源于北斗文化、河洛之理、黄老医学之“多言数穷,不如守中”、《素问》运气理论、以及《灵枢》之《九宫八风》、《岁露论》、《九针论》三篇。其独特针法理念可见一斑,临床疗效自是效如桴鼓。然不懂其理法切不可随意施针,恐伤患者,甚及医身。
简介:AIM:Toaddressissuesininteroperabilitybetweendifferentfundusimagesystems,weproposedawebeyepicturearchivingandcommunicationsystem(PACS)frameworkinconformancewithdigitalimagingandcommunicationinmedicine(DICOM)andhealthlevel7(HL7)protocoltorealizefundusimagesandreportssharingandcommunicationthroughinternet.METHODS:Firstly,atelemedicine-basedeyecareworkflowwasestablishedbasedonintegratingthehealthcareenterprise(IHE)EyeCaretechnicalframework.Then,abrowser/serverarchitectureeye-PACSsystemwasestablishedinconformancewiththewebaccesstoDICOMpersistentobject(WADO)protocol,whichcontainsthreetiers.RESULTS:Inanyclientsysteminstalledwithwebbrowser,clinicianscouldlogintheeye-PACStoobservefundusimagesandreports.Multipurposeinternetmailextensions(MIME)typeofastructuredreportissavedaspdf/htmlwithreferencelinktorelevantfundusimageusingtheWADOsyntaxcouldprovideenoughinformationforclinicians.Somefunctionsprovidedbyopen-sourceOviyamcouldbeusedtoquery,zoom,move,measure,viewOICOMfundusimages.CONCLUSION:Suchwebeye-PACSincompliancetoWADOprotocolcouldbeusedtostoreandcommunicatefundusimagesandreports,thereforeisofgreatsignificanceforteleophthalmology.
简介:AIM:Topresenttheoutcomeofmodifiedgridlaserphotocoagulation(GLP)indiffusediabeticmacularedema(DDME)ineyeswithoutextrafovealand/orvitreofovealtraction.METHODS:InclusioncriteriafortheretrospectivestudywereDDMEeyesofpatientswithtypeⅡdiabetesmellitusthathad≥4monthsoffollow-upfollowingGLP.Onlyoneeyeperpatientwasanalyzed.Using3-Dspectral-domainopticalcoherencetomography(3-DSDOCT),eyesthathadeitherextrafovealorvitreofovealtraction,orhadbeenpreviouslytreatedbyanintravitrealmedication(s)wereexcluded.TreatedDDMEeyesweredividedinto4groups:A)'Classic'DDMEthatinvolvedthecentralmacula;B)edemadidnotinvolvethemacularcenter;C)eyesassociatedwithcentralepiretinalmembrane(ERM);D)DDMEthatwasassociatedwithmacularcapillarydropout≥2disc-diameter(DD).RESULTS:GLPoutcomein35DDMEeyesafter4-24(mean,13.1±6.9)monthswasasfollows:GroupA)18eyeswith'classic'DDME.Followingoneor2(mean,1.2)GLPtreatments,best-correctedvisualacuity(BCVA)improvedby1-2Snellenlinesin44.4%(8/18)ofeyes,andworsenedby1linein11.1%(2/18).Centralmacularthickness(CMT)improvedby7%-49%(mean,26.6%)in77.8%(14/18)ofeyes.CausesofCMTworsening(n=4)werecommonlyexplainable,predominantly(n=3)associatedwithemergenceofextrafovealtraction,5-9monthspost-GLP.GroupB)GLP(s)inDDMEthatdidnotinvolvethemacularcenter(n=6)resultedinimprovedBCVAby1-2linesin2eyes.However,thecentralmaculabecameinvolvedintheedemaprocessaftertheGLPin3(50%)eyes,associatedwithanemergenceofextrafovealtractioninoneoftheseeyes4monthsfollowingtheGLP.GroupC)GLPfailedinall5eyesassociatedwithcentralERM.GroupD)GLPwasofpartialbenefitin2of6treatedeyeswithmacularcapillarydropout≥2DD.CONCLUSION:EyeswithDDMEthatinvolvedthemacularcenterwerefoundtoachievefavourableoutcomesafterGLP(s)duringmid-termfollow-up,unlesscomplicatedpre-GLPorpost-GLPbyvltreoretinalinterfaceabnormalities,oftenextrafovealtra