Screening and Primary Characterization of New Antigen Genes of Schistosoma Japonicum-中国期刊网

摘要 TofindSchistosomajaponicum(S.j)newantigengenethusprovidemoreusefulvaccinecandidates,thecDNAlibraryofS.jadultwormwasscreenedwithseraofrabbitsimmtmizedwiththemembraneantigensofSchistosomajaponicumhepato-portalschistosomula(SjHmAg).ThepositivecloneswereamplifiedbyPCRandsequenced,thenthesequencesofcloneswerecomparedwithallsequencesinGenBankdatabaseusingBlastprocess.ThenewclonesweresubmittedtoGenBankforaccessionnumbers.Fifteenpositivecloneswereobtainedafterthreeroundsofimmunoscreening.ThesizeofS.jcDNAfragmentsinpositiveclonesrangedfrom0.7kb-3.0kbafterautomaticallyexcisedwiththehelperphage.SequenceanalysisrevealedthatpartialsequenceofcloneM5hadsignificanthomologywithS.jmitochondriarnRNA,theotherpositivecloneswerenewS.jgenes.M2clonesequence(GenBankaccessionnumberAF502579)was730bplongithada117bpopenreadingframe(ORF).ThesequenceofM15(GenBankaccessionnumberAF502582)hasnotransmembraneregionandencodes92aminoacids,anditsproteincontainsaferredoxinsiron-sulfurbindingregionsignatureandtwoVWFCsignalregions.ThesizeofM1,M8,M9,M12(GenBankaccessionnumbers:AF502578,AF502580,AF500622,AF502581)rangesfrom402bpto766bp.ItconcludedthattheserafromrabbitimmunizedwithSjHmAgcouldrecognizeS.jspecificantigensmolecules,andtheseantigensmayinducetheprotectiveimmunityagainstS.jinfection.

1陈欲晓;易新元;曾宪芳;袁仕善;张顺科;LarryMCREYNOLDS. Isolation and Characterization of 44.6 kDa Protein from Schistosoma japonicum Male Worm.基础医学,2004-02.
2Jing Xu,Shi-Zhu Li,Jia-Gang Guo,Xiao-Nong Zhou,Amadou Garba Djirmay. The WHO new guideline to control and eliminate human schistosomiasis: implications for the verification of transmission interruption and surveillance of Schistosoma japonicum in China.医药卫生,2022-12.
3王敏;易新元;曾宪芳;李先平;周东明;张顺科;章洁. Selection of the Mimic Epitopes of Antigens from Schistosoma japonicum by Using Phage Display Techniques.基础医学,2003-01.
4Li Wang;Peng Li;Xiong Guo. Screening of methylation genes in age-related cataract.眼科,2018-07.
5Yin-Long Li,Hui Dang,Su-Ying Guo,Li-Juan Zhang,Yun Feng,Song-Jun Ding,Xiao-Wei Shan,Guang-Ping Li,Min Yuan,Jing Xu,Shi-Zhu Li. Molecular evidence on the presence of Schistosoma japonicum infection in snails along the Yangtze River, 2015-2019.医药卫生,2022-12.
6陈欲晓;易新元;曾宪芳;王林纤;唐连飞;章洁;McreynoldsLarry. Selection of Immunogenic Peptide Mimics of Male Worm Origin of Schistosoma Japonicum using Phage Display Peptide Library.基础医学,2004-01.
7Shen Hang,Xu Zhi-Xin,Li Qi-Fu. New advances in the diagnosis of primary aldosteronism.医药卫生,2020-08.
8. Identification and Characterization of 177 Unreported Genes Associated with Liver Regeneration.分子生物学,2004-02.
9Zheng Jin-Xin,Xia Shang,Lv Shan,Zhang Yi,Bergquist Robert,Zhou Xiao-Nong. Infestation risk of the intermediate snail host of Schistosoma japonicum in the Yangtze River Basin: improved results by spatial reassessment and a random forest approach.医药卫生,2021-08.
10Raafat El-Namaky;Saber Sedeek;Yonnelle Dea Moukoumbi;Rodomiro Ortiz;Baboucarr Manneh. Microsatellite-Aided Screening for Fertility Restoration Genes (Rf) Facilitates Hybrid Improvement.作物学,2016-03.

Screening and Primary Characterization of New Antigen Genes of Schistosoma Japonicum

来源期刊

相关推荐

同分类资源更多

相关关键词

Screening and Primary Characterization of New Antigen Genes of Schistosoma Japonicum

来源期刊

相关推荐

同分类资源 更多

相关关键词

同分类资源更多