简介:InordertorevealvariationandrevolutionofNPgenesofhumanavianH_5N_1influenzavirusstrains,theNPgeneofahumanavianH_5N_1influenzavirusstraininGuangdongwassequencedandtheglobalNPgenesofstrainswereretrieved.ThesequenceswereanalyzedbyDNAStar5.0,andtheevolu-tionaryspeedwasstudiedwithreferencetotheepidemiologicaldata.ItwasfoundthatNPgenesof45strainsduring1997-2006werehomologicallyclassifiedintothreegroups:strainsin1997-1998,strainsin2004-2005andstrainsfrom2003to2006.Therewere35substitutionsinNPsinallstrainsaccountingforaratioof7.03%(35/498).Anadditionalglycoproteindomain(NGT_(430-432))wasfoundinNPgenesinthestrainsof2003-2006,themutationofN_(370)SinGD-01-06resultedinoccurrenceofonemoreglyco-proteindomain(NES_(368-370)).Inthesynonymousvariation,K_svaluesinNPwere2.03×10~(-5)-2.55×10~(-5)Nt/dandK_avaluesinNPwere1.58×10(-6)-3.10×10~(-6)Nt/d.Theredidn′texistobviouslyselectivepres-sure.Anadditionalglycoproteindomainineverystrainof2003-2006andonemoreinstrainGD-01-06mightchangetheantigenicityofhumanavianH_6N_1influenzavirus.ThevariationonhumanavianH_5N_1influenzastrainsoccurredfrequentlyinthenaturalworld,whichwouldresultinhighprobabilityofhu-man-humantransmissionalongwiththenaturalevolutionofthevirus.
简介:ToinvestigatethephenotypicknockoutofHIV-1chemokinecoreceptorCXCR4andCCR5byintrakinesanditsinhibitoryeffectonHIV-1infection.PrimaryhumanPBLsweretransducedwiththerecombinantvectorpLNCX-R-K-S-K(△NGFR),followedbyanti-NGFR/anti-IgG-magneticbeadmethodselectionandFCMdetection.ThetransducedPBLswereinfectedwithDP1HIV-1virusthereafterenvelope-mediatedsyncytiumformationandp24detectionwerecarriedouttostudytheblockageofHIV-1infectionbyco-inactivationofCCR5andCXCR4.pLNCX-R-K-S-K(△NGFR)-transducedPBILswereisolatedwithananti-NGFR/anti-IgG-magneticbeadmethod.Afterisolation,about70%ofthePBLswerepositivefortheNGFRmarker.WhenthetransducedPBLswereinfectedwithDP1HIV-1virus,envelop-mediatedsyncytiumformationwasalmostcompletelyinhibitedbypLNCX-R-K-S-K(△NGFR)transfection.Also,p24antigenwasverylowintheculturesofpLNCX-R-K-S-K(△NGFR)transducedPBLs.pLNCX-R-K-S-K(△NGFR)transductioninhibitedtheproductionofDP1p24antigenby15%,43%and19%ondays4,7and10respectively.ThelymphocyteswiththephenotypicknockoutofCCR5andCXCR4couldprotectprimaryhumanPBLsfromDP1HIV-1virusinfection.
简介:Theimmuneefficiencyofarecombinantadenovirustype5withtype35fibercontainingHIV-1gaggene(rAd5/F35-mod.gag)wasinvestigatedinBALB/cmice,inwhichtherAd5/F35-mod.gagwasfirstlyidentifiedwithPCR,thentransfectedto293cellsandtheinvitroexpressionlevelofGagproteinwasdeterminedbyWesternblottingandindirectimmuno-fluorescentassay.MicewereimmunizedwithintramuscularinjectionsofrAd5/F35-mod.gag,rAd5-mod.gagorDNAandwereboostedafter3weeks.Totesttheeffectofpre-existinganti-viralimmunityonimmunization,micewerealsoinjectedwithAd5-GFPvectorandthenimmunized4and7weekslaterwithAd5/F35-mod.gagvector.TheP24-specificIgGantibodyinseraofimmunizedmicewasdeterminedbyELISAandthespecificcytotoxicTlymphocyte(CTL)responsewasassayedbyintracellularcytokinestaining.ItwasdemonstratedthattherAd5/F35-mod.gagvectorcouldexpressefficientlytheHIVGagproteinin293cellsinvitroandinducestrongHIV-specificimmuneresponsesinvivo.ThestrongestCTLandserumIgGresponseoccurredwhenmicewereimmunizedtwicewithinjectionofrAd5/F35alone,buttheanti-Ad5antibodyafterprimaryinfectionwithadenoviruscouldinhibitthespecificimmuneresponsesinducedbyrAd5/F35vector.ItisconcludedthatsingleimmunizationwithrecombinantadenovirusrAd5/F35-mod.gagcaninducespecificCTLandserumIgGantibodyresponsesinmice,buttheimmunogenicityofrAd5/F35iscomparablyweakerthanthatofrAd5.
简介:【摘要】 目的 探讨单用阿比多尔及阿比多尔序贯使用洛匹那韦两种方案治疗普通型新型冠状病毒肺炎时对肝功能的影响及疗效的分析。 方法 回顾性分析2020年上半年某医院161名普通型新型冠状病毒肺炎患者资料,其中男性75例,女性86例,平均年龄56岁。分析不同的抗病毒治疗方案对患者肝功能的影响及其治疗效果的比较。 结果 两组治疗方案均有助于恢复患者合成白蛋白及谷丙转氨酶的功能,其中观察组方案优于对照组(P<0.05),两组方案对谷草转氨酶、胆红素的合成、排出及凝血功能紊乱改善不明显,无统计学意义(P>0.05)。对患者住院天数,观察组亦略少于对照组,可见随着年龄增加,患者住院天数增多(
简介:【摘要】2020年初,在湖北武汉爆发了新型冠状病毒感染的肺炎疫情,全国30个省启动了重大突发公共卫生事件一级响应。绵竹市为进一步做好全市医疗卫生应对呼吸道相关重大传染病的防控工作,提高对新冠肺炎防治水平和应对能力,切实有效地防止新冠肺炎疫情的扩散蔓延,提出了建立疫情防控的“1+6+N”三级医疗救治体系。本文以孝德卫生院围绕“1+6+N”开展的系列工作为例,阐述了“1+6+N”体系在疫情防控中的作用。
简介:【摘 要】目的:探讨 5-氨基酮戊酸动力疗法治疗寻常型痤疮过程中综合护理的应用价值。方法:对 2018年 1 月 -2019年 1 月我院收治的 150例寻常型痤疮患者分成两组进行研究,依据的是红蓝球法。全部病例的治疗均给予 5-氨基酮戊酸动力疗法,其中 75例对照组运用的是常规护理, 75例试验组运用的是综合护理,进行两组临床效果的比较。结果:对照组与试验组经治疗及不同的护理后,试验组的总有效率要比对照组高,两组有统计学差异( P< 0.05)。结论:寻常型痤疮接受 5-氨基酮戊酸动力疗法治疗期间辅助综合护理,有利于进一步改善病情和提高临床疗效,有推广和借鉴的价值。
简介:【摘要】目的: 探究心理护理对 1 型糖尿病患者负性心理情绪的影响以及对血糖水平的控制情况。 方法: 将我院收治的 1 型糖尿病患者 70 例纳入研究,以随机抽样法分为两组,两组均采用相同治疗,并给予常规护理,观察组同时加强心理护理,对比两组患者的血糖水平,以 SCL-90 评分量表评估患者的负性心理改善情况。 结果: 观察组患者的 SCL-90 量表各项指标均评分低于对照组( P<0.05 ),观察组患者的血糖水平低于对照组( P<0.05 ),存在统计学意义。 结论: 1 型糖尿病患者应用心理护理干预,可改善负性心理情绪,控制血糖水平,效果显著。