简介:把动物红细胞输用给人,即异种输血,可以缓解目前血源紧张的矛盾,但牛和猪的红细胞与人的血浆发生强烈的凝集反应,不能直接输用.本研究首次对牛和猪红细胞表面抗原进行修饰改造,并对改造后的猪、牛红细胞进行比较,从而为开拓丰富来源、安全有效的人血代用品奠定基础.使用基因重组的咖啡豆α-半乳糖苷酶清除牛和猪红细胞表面的主要异种抗原-α-Gal抗原,结合使用化学材料甲氧基聚乙二醇(mPEG)修饰遮蔽牛和猪红细胞表面的非主要异种抗原-non-αGal抗原,以实现对牛和猪红细胞表面异种抗原的改造.结果:改造后的牛、猪红细胞与人混合血浆的盐水凝集反应消失,具有和人红细胞相似的血清学特征;牛红细胞比猪红细胞脆性低,异种抗原的表达量低,及其他的一些特征说明牛红细胞比猪红细胞更适合作为人红细胞代用品.结论:改造后的牛红细胞与人血浆相匹配,有可能成为人红细胞代用品.
简介:目的探讨在ConA诱导肝细胞损伤过程中的Fas抗原的表达情况以及CsA干预对其损伤的影响。方法尾静脉注射ConA(20mg·kg^-1)于BALB/c小鼠作为试验组;提前半小时予以CsA(25mg·kg^-1)后再按试验组处理作为CsA组。观察血清中ALT、AST含量动态变化及肝组织细胞Fas抗原表达。结果试验组血清中ALT、AST进行性升高,在12h时与对照组、CsA组比较均P<0.01。试验组有大量Fas抗原表达的肝细胞,且数量逐渐增多、信号逐步增强。对照组和CsA组血清中ALT、AST变化不大,肝细胞Fas抗原表达不明显。结论Fas配体-抗原系统介导了ConA所致肝损伤,细胞毒性T淋巴细胞的激活是其肝细胞损伤的重要机理。
简介:AbstractBackground:Differential diagnosis of active tuberculosis (ATB) and latent tuberculosis infection (LTBI) has been a challenge for clinicians in high TB burden countries. The purpose of this study was to improve the accuracy of differential diagnosis of ATB and LTBI by using fluorescent immunospot (FluoroSpot) assay to detect specific Th1 cell immune responses. The novel mycobacterium tuberculosis (MTB) latency-associated antigens Rv1733c and synthetic long peptides derived from Rv1733c (Rv1733c SLP) were used based on virulence factors early secreting antigen target-6 (ESAT-6) and culture filtrate protein-10 (CFP-10).Methods:Fifty-seven ATB cases, including 20 pathogen-confirmed ATB and 37 clinically diagnosed ATB, and 36 LTBI cases, were enrolled between January and December 2017. FluoroSpot assay was used to detect the interferon γ (IFN-γ) and interleukin 2 (IL-2) secreted by the specific T cells after being stimulated with MTB virulence factors ESAT-6 and CFP-10, MTB latency-associated antigens Rv1733c and Rv1733c SLP. The receiver operating characteristic (ROC) curve was used to define the best cutoff value of latency-associated antigens in the use of differentiating ATB and LTBI. The sensitivity, specificity, predictive value, and likelihood ratio of ESAT-6 and CFP-10-FluoroSpot combined with latency-associated antigen in the differential diagnosis of ATB and LTBI were also calculated.Results:Following the stimulation with Rv1733c and Rv1733c SLP, the frequency of single IL-2-secreting T cells stimulated by Rv1733c SLP had the largest area under the ROC curve, which was 0.766. With a cutoff value of 1 (spot-forming cells [SFCs]/2.5 × 105 peripheral blood mononuclear cells) for frequency, the sensitivity and specificity of distinguishing ATB from LTBI were 72.2% and 73.7%, respectively. ESAT-6 and CFP-10-FluoroSpot detected the frequency and proportion of single IFN-γ-secreting T cells; the sensitivity and specificity of distinguishing ATB from LTBI were 82.5% and 66.7%, respectively. Combined with the frequency of single IL-2-secreting T cells stimulated by Rv1733c SLP on the basis of ESAT-6 and CFP-10-FluoroSpot, the sensitivity and specificity increased to 84.2% and 83.3%, respectively.Conclusion:Rv1733c SLP, combined with ESAT-6 and CFP-10, might be used as a candidate antigen for T cell-based tuberculosis diagnostic tests to differentiate ATB from LTBI.
简介:Themoditicationoftumorcellsoreffcorcellsusingcytokinegenesasastrategytoenhancehostantitumorimmunityhasbeenstudiedintensivelyoverthe
简介:Objective:Toanalyzesubtypesandquasi-speciesofisolatedvirusesfromHIV-1infectedindividualsamongthepopulationepidemiologicaldynamicsoflocalHIV-1isolates,thuslayingafoundationfordesigningacandidateAIDSvaccine.Methods:Byhetero-duplexmobilityassay(HMA)andsinglestrandconformationpoly-morphism(SSCP)analysisonampliconsfromsingle-primedpolymerasechainreaction(SP-PCR),subtypesandquasi-speciesoftestedHIV-1isolateswereelucidated,andampliconsweresequencedforconfirmation.Results:Specificampliconsfromdifferentsubtypesandquasi-speciesofHIV-1couldbediscerniblebyHMAandSSCPanalysis.HIV-1isolatesfromdifferentpatientsmightbeeitheradifferentsrbtypeoranidenticalsubtype,andHIV-1isolatesfromanindividualwerepresentinapopulationofquasi-species.
简介:摘要目的探讨血清微小核糖核酸-34a(miR-34a)、癌抗原15-3(CA15-3)和癌抗原125(CA125)联合检测在乳腺癌复发转移监测中的价值。方法根据患者影像学及病理组织学检查将2016年1月至2020年12月武汉科技大学附属普仁医院诊治的96例女性乳腺癌患者分为复发转移组(39例)与未复发转移组(57例),实时荧光定量反转录-聚合酶链反应(qRT-PCR)检测血清miR-34a相对表达量,酶联免疫吸附法检测血清CA15-3和CA125水平。采用受试者操作特征曲线(ROC)分析血清miR-34a、CA15-3和CA125及三者联合检测对乳腺癌患者术后复发转移监测的作用。计量数据比较采用t检验,计数资料比较采用χ2检验。结果复发转移组乳腺癌患者血清miR-34a相对表达量显著低于未复发转移组乳腺癌患者(miR-34a相对表达量0.41±0.04比0.97±0.06),血清CA15-3和CA125水平显著高于未复发转移组乳腺癌患者[CA15-3为(79.62±3.77) U/ml比(14.89±2.14) U/ml,CA125为(152.56±13.77) U/ml比(19.32±2.13) U/ml],差异均有统计学意义(t=20.807、9.478、36.663,P值均<0.05)。血清miR-34a预测乳腺癌患者复发转移的敏感度为87.4%,特异性为69.5%;血清CA15-3预测乳腺癌患者复发转移的敏感度为89.1%,特异性为72.5%;血清CA125预测乳腺癌患者复发转移的敏感度为72.4%,特异性为82.7%。miR-34a、CA15-3和CA125三者联合检测敏感度为91.8%,特异性为89.1%。结论乳腺癌复发转移患者血清miR-34a表达显著降低,血清CA15-3和CA125水平显著升高,三者联合检测在乳腺癌复发转移监测中具有重要临床价值。