简介：〈七绝夏夜〉孤枕浮心梦不长,夏蛙声咽使天凉。离愁挥去成朝露,应恨窗花不肯香。＂我说你个老头子,有话不能慢慢说,还动起手了？小洁如果出什么事,看我和你没完不……哎,哎,老头子,你怎么了？你怎么了？＂洁母看到女儿哭着跑出去,心疼代替了是非,只见她从衣架上摘下一条围巾,一边责备洁父,一边想追出去给洁。

简介：摘要目的了解河南地区部分归国人员恶性疟疾患者的临床特点及治疗方法。方法回顾性分析我院2010年元月-2011年8月重症医学科收治的19例输入性恶性疟疾患者的临床特点、治疗方法、转归。结果19例患者均有不同程度的发热（100%），其他临床表现复杂。用青蒿素为基础的综合治疗，18例存活，1例死亡。结论早期确诊、病原治疗、支持对症治疗是抢救恶性疟疾成功的根本措施。

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简介：有西方的音乐评论家做过这样的描述：“如果说歌剧作品是浓墨重彩写意的油画，那么艺术歌曲则是精雕细琢的工笔或清新雅致的山水画”。艺术歌曲以其简洁严谨的曲式结构、优美的语言、高度的艺术性，成为理想的音乐会曲目及声乐教材。像贝里尼这样的歌剧大师所创作的艺术歌曲是重要的音乐文献。虽然为数不多，但各有其独到之处。在已经出版的国内外作品集里，我们可以找到19首。每一首作品都有着贝里尼音乐中所呈现出的精致的旋律，细腻的情感。

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简介：AbstractBackground:The pandemic of the coronavirus disease 2019 (COVID-19) has caused substantial disruptions to health services in the low and middle-income countries with a high burden of other diseases, such as malaria in sub-Saharan Africa. The aim of this study is to assess the impact of COVID-19 pandemic on malaria transmission potential in malaria-endemic countries in Africa.Methods:We present a data-driven method to quantify the extent to which the COVID-19 pandemic, as well as various non-pharmaceutical interventions (NPIs), could lead to the change of malaria transmission potential in 2020. First, we adopt a particle Markov Chain Monte Carlo method to estimate epidemiological parameters in each country by fitting the time series of the cumulative number of reported COVID-19 cases. Then, we simulate the epidemic dynamics of COVID-19 under two groups of NPIs: (1) contact restriction and social distancing, and (2) early identification and isolation of cases. Based on the simulated epidemic curves, we quantify the impact of COVID-19 epidemic and NPIs on the distribution of insecticide-treated nets (ITNs). Finally, by treating the total number of ITNs available in each country in 2020, we evaluate the negative effects of COVID-19 pandemic on malaria transmission potential based on the notion of vectorial capacity.Results:We conduct case studies in four malaria-endemic countries, Ethiopia, Nigeria, Tanzania, and Zambia, in Africa. The epidemiological parameters (i.e., the basic reproduction number R0 and the duration of infection DI) of COVID-19 in each country are estimated as follows: Ethiopia (R0 = 1.57, DI = 5.32), Nigeria (R0 = 2.18, DI = 6.58), Tanzania (R0 = 2.47, DI = 6.01), and Zambia (R0 = 2.12, DI = 6.96). Based on the estimated epidemiological parameters, the epidemic curves simulated under various NPIs indicated that the earlier the interventions are implemented, the better the epidemic is controlled. Moreover, the effect of combined NPIs is better than contact restriction and social distancing only. By treating the total number of ITNs available in each country in 2020 as a baseline, our results show that even with stringent NPIs, malaria transmission potential will remain higher than expected in the second half of 2020.Conclusions:By quantifying the impact of various NPI response to the COVID-19 pandemic on malaria transmission potential, this study provides a way to jointly address the syndemic between COVID-19 and malaria in malariaendemic countries in Africa. The results suggest that the early intervention of COVID-19 can effectively reduce the scale of the epidemic and mitigate its impact on malaria transmission potential.

简介：AbstractThis study aimed to determine the clinical characteristics of patients diagnosed with the coronavirus disease 2019 (COVID-19). Clinical data of COVID-19 patients diagnosed between January 28, 2020 and February 23, 2020 at the Beijing You'an Hospital were summarized and analyzed. Overall, 45 (18 men and 27 women) patients were included in this study. The average age of patients was 58 years (range, 7–94 years). Furthermore, 21 patients (47%) experienced underlying chronic diseases, with another four patients (9%) having three or more chronic diseases simultaneously. The first symptoms appeared at the onset of illness onset include fever in 36 patients (80%), cough in 23 patients (51%), and expectoration in 15 patients (33%), respectively. Patients may experience hepatic and renal injury as well as abnormal myocardial enzymes in varying degrees. Senior patients (≥58) and accompanying chronic diseases were considered as independent predictors for developing a severe and critically ill population with increased mortality. Laboratory results regarding the NEU percentage, NLR, ALC, and C-reactive protein levels were considered significant in predicting clinically critical disease or for prognosis assessment and thus require further studies. COVID-19 may affect multiple organs of the human body. Glucocorticoid is considered effective in the treatment of patients diagnosed with severe COVID-19.

简介：摘要目的通过检测79例COVID-19患者血清新型冠状病毒(2019-nCoV)特异性IgM和IgG抗体水平，了解这两种抗体在患者体内的变化规律。方法选取杭州市西溪医院临床确诊为COVID-19的患者79例，收集不同时期(发病后≤10 d、10<～20 d、20<～30 d、>30 d)血清样本共167份，化学发光免疫分析法检测2019-nCoV特异性IgM和IgG抗体水平并结合临床资料进行分析。结果重型和普通型患者IgM抗体平均水平高于轻型患者[21.77 (10.18～128.65)和13.13 (6.08～35.14) vs 3.01 (1.69～8.69)；χ2＝27.442，P<0.01]。重型和普通型患者IgG抗体平均水平高于轻型患者[(124.22±36.79)和(120.04±63.25)vs(52.31±53.68)，F/χ2＝27.295，P<0.01)。康复出院后，重型和普通型患者的IgM、IgG抗体阳性率均高于轻型患者(P<0.01)。IgM和IgG抗体水平受检测时间影响，发病后10<～20 d的IgM抗体水平明显高于≤10 d的水平(P<0.05)，发病后>10 d的IgG抗体水平明显高于≤10 d的水平(P<0.01)。结论重型COVID-19患者体内能检测到更高水平的IgM和IgG抗体，且IgM和IgG抗体水平与患者发病后的检测时期密切相关。

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简介：湖北省15家企业19个项目获国家批准，在未来5年中将逾2000万吨二氧化碳的减排指标卖给国际买家，交易额高达16亿元．这19个项目涉及工业节能降耗，可再生能源开发、燃料替代等领域。其中，武钢4个项目每年250多万吨的二氧化碳减排量，被意大利国家电力公司购得，年获利达2亿元。

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简介：目的研究细胞角蛋白20mRNA（CK20mRNA）在腹腔镜结直肠癌手术患者外周血中的表达情况，探讨腹腔镜治疗结直肠癌手术对肿瘤微转移的影响。方法将44例结直肠癌患者采用单盲随机方法分为腹腔镜组25例与开腹组19例，采用逆转录聚合酶链反应检测患者术前、术中、术后外周血中CK20mRNA的表达，对132例次标本进行CK20mRNA检测的研究。结果腹腔镜组CK20mRNA阳性表达25例次，阴性表达50例次；开腹组：CK20mRNA阳性表达22例次，阴性表达35例次；2组间比较无统计学意义。结论腹腔镜结直肠癌手术患者外周血术前、术后CK20mRNA表达与开腹手术无统计学意义。腹腔镜结直肠癌手术在肿瘤微转移方面是安全、可行、有效的。

简介：摘要：针对桁架钢筋混凝土叠合板和预制钢筋混凝土板式楼梯在广西壮族自

简介：摘要目的构建结核分枝杆菌（MTB）16kD-38kD-19kD融合基因重组质粒，并对融合蛋白进行表达及纯化。方法利用PCR方法分别扩增16kD、38kD及19kD基因，经过双酶切依次与pET28a载体连接，构建pET28a-16kD-38kD-19kD融合基因重组质粒，转化至大肠杆菌表达株BL21中，经IPTG诱导获得目标蛋白，并进行镍柱纯化。结果经过酶切及测序鉴定证实pET28a-16kD-38kD-19kD重组质粒构建正确，并经原核表达及纯化获得融合蛋白。结论成功构建并表达纯化16kD-38kD-19kD融合蛋白，为结核病特异性诊断抗原的开发奠定了基础。

简介：摘要嵌合抗原受体T细胞（CAR-T）免疫疗法是治疗血液系统肿瘤的有效方法，尤其在B细胞血液肿瘤中表现突出。虽然在临床中观察到较高的缓解率，但大部分患者在CAR-T治疗后仍会复发。随着CAR-T疗法在B细胞肿瘤和其他血液恶性肿瘤中的广泛应用，需要对其复发机制和治疗策略进行进一步的临床研究，以提升临床疗效，改善患者预后。

简介：摘要CD19嵌合抗原受体T细胞疗法是治疗B细胞血液肿瘤的新兴免疫疗法，并取得了较好的疗效。随着其使用的增加，免疫效应细胞相关细胞因子释放综合征的发生率也相应升高，迫切需要对其精确机制和治疗进行进一步的临床研究。文章总结了细胞因子释放综合征的机制、临床表现、分级系统、治疗以及管理策略。

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简介：摘要目的探讨顺铂对肝癌细胞衰老的诱导作用。方法使用2 μg/ml顺铂处理人肝癌细胞系HepG2，采用MTS法检测细胞增殖、流式细胞系检测细胞周期变化、RT－PCR和Western blot检测衰老相关基因p19、p21表达量、免疫荧光法检测p21表达情况；裸鼠移植人肝癌模型腹腔注射相应药物，检测肝癌生长转移及p21蛋白表达。结果随着顺铂处理时间延长，顺铂组增殖率较对照组降低，差异有统计学意义(t＝8.958，P<0.05)。流式细胞检测显示，顺铂组和对照组G2期细胞分别为19.90%±0.42%、12.57%±0.84%，细胞停滞于G2期(t＝14.415，P<0.05)。顺铂组与对照组p19基因相对表达量分别为(2.23±0.05)、(1.00±0.02)；p21基因相对表达量分别为(3.26±0.11)、(1.00±0.02)，p19及p21表达均升高(分别t＝43.750，56.541，均P<0.05)；顺铂组裸鼠瘤体体积较对照组缩小，p21蛋白表达水平较对照组增加(P<0.05)。结论顺铂可通过p19/p21相关途径诱导肝癌细胞衰老。