简介:TheCurietemperatureof(Nd1-xPrx)2Fe14CoyBcompounds(x=0,0.2,…,1.0andy=O,2,4,6,14)hasbeendeterminedwithACinitialsusceptibilitymeasurement.ItwasfoundthatthevariationsofCurietempera-turewithxisnotlinearfory=0and14,whereasitislinearforothervaluesofy.Thelatticeconstantsof(Nd1-xPrx)2Fet14BweredeterminedbyusingX-raydiffraction.Thelatticeexpansionwasmostlyalongthecaxis,whereasthatalongthea-axisremainedpracticallyzeroforthewholeseries.ItissuggestedthatPratomsmayshowsitepreferenceinR2M14Bcompounds(M=FeorCo).DuetothesubstitutionofCoforFe.thetendencyofsitepreferenceofPrbecomeslesspronounced,whichmaybeattributedtothedecreaseofdiffer-enceofcrystalelectricfield(CEF)actingonthetworareearthsiteswiththeintroductionofCoorFe.Incon-trasttothatofthePratoms,thesitepreferenceofCoiu(Nd(1-x)Prx)2Fe(14-y)CoyBcompoundsdoesnotdependonthecompositionoftherareearthsublattice(R=NdorPr).
简介:Objective:TodeterminewhetherInterferon-alpha-2b(IFN-α2b)canmodulatetheautophagicresponseinhepatocellularcarcinomacells.Methods:HepatocellularcarcinomacellsweretreatedwithIFN-α2b.Autophagywasassessedbyacridineorangestaining,GFP-LC3dottedassay,transmissionelectronmicroscopyandimmunoblotting.Results:AcridineorangestainingshowedthatIFN-α2btriggeredtheaccumulationofacidicvesicularandautolysosomesinHepG2cells.TheacridineorangeHepG2cellratioswere(4.3±1.0)%,(6.9±1.4)%,and(13.1±2.3)%,respectively,aftertreatmentwith100,1,000,and10,000IU/mLIFN-α2bfor48h.AmarkedlypunctatepatternwasobservedinHepG2cellstreatedwith10,000IU/mLIFN-α2bfor48h,butonlydiffuseandweaklyfluorescentGFP-LC3punctawasobservedincontrolcells.HepG2cellstreatedwith10,000IU/mLIFN-α2bfor48hdevelopedautophagosome-likecharacteristics,includingsingle-ordouble-membranevacuolescontainingintactanddegradedcellulardebris.TheBeclin1andLC3-IIproteinexpressionwasup-regulatedbyIFN-α2btreatment.Conclusion:Autophagycanbeinducedinadose-dependentmannerbytreatmentwithIFN-α2binHepG2cells,andtheBeclin1signalingpathwaywasstimulatedbyIFN-α2b.
简介:瞄准:检验在精子DNA损坏和精子之间的关系原子嘘染色的(H2B)。方法:我们从14连续asthenoteratozoospermic评估了精子样品不肥沃的人和六连续肥沃的控制。精子原子嘘染色的(H2B)和精子染色质正直(由精子染色质结构试金估计了并且表示了使用(i)DNA破碎索引的百分比[%DFI]并且(ii)高DNA污点能力[%HDS)])被评估。结果:HistoneH2B免疫细胞化学表明了二个原子染色模式:(i)焦点的有小斑点的染色;并且(ii)扩散染色。不肥沃的人有展出弥漫的H2B染色比的精子的一个更高吝啬的百分比做了肥沃的人(7.7%±4.6%vs.1.6%±1.2%,分别地P<0.01)。我们观察了在精子的比例之间的重要关系与弥漫原子嘘一染色和两精子%DFI(r=0.63,P<0.01)并且精子%HDS(r=0.63,P<0.01)。结论:数据证明不肥沃的人有精子的一个更高的比例与弥漫嘘一H2B与做肥沃的人并且建议精子DNA损坏力量比,至少部分地,由于反常地高嘘一个H2B层次。
简介:瞄准:在老鼠男性检验不可分的膜蛋白质2b(Itm2b)的表示和规定繁殖纸巾在性成熟期间并且在不同治疗由下面在situ杂交。方法:睾丸,epididymis,和管推迟ens在天1-70上被收集在性成熟期间检验Itm2b表示。为了推进,检验Itm2b的规定,成年老鼠经历了外科的阉割和cryptorchidism。乙烯暗淡乙烷sulfonate和busulfan治疗被执行在Leydig房间和细菌房间的破坏以后测试Itm2b的规定。结果:在睾丸,Itm2b表示中等在天1-10上在生精的绳索的广告钠区域被检测,并且在天在spermatogonia在底层检测了20和30。Itm2b水平显著地从到白天70的白天20在Leydig房间被增加。在epididymis和管推迟ens,Itm2b从出生不满一月的婴儿被检测到逐渐地根据性成熟增加的成年人,和信号。Itm2b表示是显著地击倒在epididymis和管调整了推迟阉割的老鼠的ens,并且当阉割的老鼠与睾丸激素被对待时,强烈刺激了。Cryptorchidism在睾丸和头epididymis导致了Itm2b表示的重要衰落。在epididymis和管的Itm2b表示推迟在Leydig房间被乙烯破坏以后,ens显著地被减少暗淡乙烷sulfonate。Busulfan处理没在epididymis或管在Itm2b表示生产明显的变化推迟ens。结论:我们的数据建议那个Itm2b表达式起来在老鼠男性复制由睾丸激素和力量玩调整了一个角色。
简介:Inthiswork,DSCandSEMstudiesindicatethation-ligandinteractioncanbeutilizedtoenhancetheinteractionofpoly(styrene-block-2-vinylpyridine)[P(S-b-2VP)]andpolyethylenebasedionomer(Surlyn).Thecompatibilityforthisblendingsystemcanbeimprovedbythisspecialinteractionand20/80wtistheoptimumblendingcompositionwithgoodcompatibility.FTIRresultsfurthercertifythatstronginteractionsexistintheblendingsystem.
简介:TwoseriesofterritremBanalogues(10a-10cand18)havebeendesignedandsynthesizedfromjujubogenin5awhichwaspreparedfromjujubogeninglycosides5bobtainedfromtheleavesofZizyphusjujuba.Thestructuresofthenewcompoundswereconfirmedby1H-,13C-NMRandMSdata.Compounds10cand18showedweakinhibitoryeffectonAChEat10^-4mol/E.