简介:摘要目的探讨在基层医院,通过采用优质护理,提高手足口病的诊治成功率。方法对我院2014年1月-7月份住院的628例手足口病患儿进行优质护理,包括做好手足口病患儿宣传教育和疫情报告、切断传播途径、情绪和心理干预,同时密切观察患儿病情,争取尽早识别重症病例,并对我院住院的98例重症患儿的护理经验进行总结。结果530例手足口病(普通型)患儿全部治愈,98例重症患儿中,有5例危重型手足口病患儿转上级医院救治,其余93例患儿经住院系统治疗后各项生理指标均恢复正常,痊愈出院。普通型患儿平均住院6.2d;重症患儿平均住院8.7d。结论及时有效的优质护理干预、不断完善管理制度并落实到位,明显提高手足口病治愈率。
简介:摘要目的探讨自然流产与染色体异常的关系。方法抽取628对自然流产夫妇的1ml肝素抗凝外周静脉血,进行淋巴细胞培养,常规G显带,计算机软件进行核型分析,计数30个核型,异常核型者计数加倍,分析5个核型。结果628对自然流产夫妇中,检出染色体异常86例,异常检出率6.8%(86/1256),其中平衡易位28例(罗伯逊易位4例),9号臂间倒位15例,Y倒位2例,1号和16号次缢痕增加20例,XXX综合征2例,特纳综合征嵌合体1例,小Y6例,随体增加7例,13号和15号短臂增加5例。在86例染色体异常中,男性异常者40例,女性异常者46例,无一对夫妇同时存在染色体异常。结论染色体异常是导致自然流产最重要的原因之一。对于有自然流产史的夫妇,都应进行双方染色体检查,有组于病因的分析和诊断,并为生育指导提供重要的依据。
简介:摘要目的探讨无创产前检测(non-invasive prenatal testing,NIPT)对于筛查胎儿21、18、13号染色体三体及基因组拷贝数变异(copy number variation,CNV)的价值。方法选取40 628例单胎孕妇作为研究对象,利用高通量测序以及生物信息学分析对母体血浆中的胎儿游离DNA进行检测,为高风险孕妇提供介入性产前诊断,对低风险孕妇进行电话随访。结果最常见的检测指征依次为血清学筛查临界风险、血清学筛查高风险以及高龄。共检出21、18、13号三体高风险257例(分别为170、49、38例),其中227例接受了介入性产前诊断,分别确诊122例、28例、10例,阳性预测值(positive predictive value,PPV)分别为81.33%(122/150)、65.12%(28/43)、29.41%(10/34)。低风险人群随访发现2例18-三体假阴性。同时NIPT检出21、18、13号染色体CNV共46例(分别为15、16、15例),其中37例接受了介入性产前诊断,分别确诊5、3、5例,PPV分别为41.67%(5/12)、25%(3/12)、33.33%(5/15)。在假阳性样本中意外发现2例其他染色体CNV。结论NIPT检测指征中血清学筛查高风险组染色体异常率较其他组高,达52.02%。NIPT对于筛查胎儿21、18、13三体具有极高的敏感度和特异度,对于筛查胎儿CNV的准确性尚有待提高。作为一项筛查手段,NIPT具有较高的临床应用价值,但存在假阳性与假阴性结果,须做好检测前后的遗传咨询工作。
简介:摘要目的探讨微小RNA(miRNA,miR)-628-3p调控的DNA解螺旋酶对骨肉瘤细胞增殖的影响。方法首先收集2013年1月至2017年12月郑州大学第二附属医院骨科收治的股骨骨肉瘤患者60例,同期收集股骨创伤性骨折患者60例作为对照组,采用定量聚合酶链反应(qPCR)检测和比较外周血循环miR-628-3p的表达水平差异,并根据随访结果进行生存分析。以人骨肉瘤细胞U2OS和HOS为研究对象,过表达miR-628-3p,采用细胞计数试剂盒(CCK-8)和集落形成实验检测其增殖活性,采用流式细胞术检测其凋亡率。根据Starbase预测miR-628-3p可与Bloom综合征解螺旋酶(BLM)结合,故进一步采用蛋白质印迹法(Western blot)、qPCR和荧光素酶报告基因实验进行检测。两组间计量资料比较采用独立样本t检验,计数资料比较采用χ2检验。结果骨肉瘤患者外周血和肿瘤组织中miR-628-3p的表达水平(相对表达量分别为0.203±0.047、0.217±0.054)均明显低于正常对照组(相对表达量分别为0.951±0.106、1.013±0.072,P<0.01),差异有统计学意义,受试者工作特征(ROC)曲线分析miR-628-3p在骨肉瘤患者中的表达,曲线下面积(AUC)=0.707(P<0.01),K-M生存分析显示miR-628-3p低表达患者生存时间明显短于高表达患者。CCK-8和集落形成实验检测显示miR-628-3p过表达后可抑制骨肉瘤细胞U2OS和NOS的增殖;流式细胞术检测显示miR-628-3p过表达后可促进骨肉瘤细胞的凋亡;qPCR和Western blot检测显示miR-628-3p过表达后,BLM的表达水平明显低于miR-628-3p nc组(P<0.01),差异有统计学意义。荧光素酶报告基因实验显示miR-628-3p mimc与BLM-mt共转染骨肉瘤细胞U2OS和NOS后,其荧光值明显低于其他转染组(U2OS:0.208±0.017比1.082±0.046,t=4.698,P<0.01;HOS:0.224±0.047比0.966±0.053,t=5.548,P<0.01),差异有统计学意义。裸鼠成瘤证实miR-628-3p过表达后肿瘤大小和重量明显低于miR-628-3p nc组[(1.419±0.274) g比(2.825±0.148) g,t=3.717,P<0.01],差异有统计学意义。结论miR-628-3p在骨肉瘤患者中存在低表达现象,与患者的不良预后和生存时间相关,miR-628-3p过表达可下调DNA解螺旋酶BLM,抑制骨肉瘤细胞的增殖。
简介:AIM:Toinvestigatetheeffectsofhydrogen-richsaline(HRS)onmicrogliaactivationandSirtuintype1(Sirt1)inratswithN-methyl-N-nitrosourea(MNU)-inducedretinitispigmentosa(RP).METHODS:Ratsweredividedintonorm(N)group,model(M)groupandHRS(H)group.RatsinMandHgroupsweregivensalineandHRSrespectivelypriortoandafteradministrationofMNU.Atoneday(d1)andd3afterwards,electroretinogramandhistologicalexaminationwereperformedtoconfirmtheeffectsofHRSonretinalfunctionandstructureofMNU-inducedRP.Immunofluorescencestainingofanti-ionizedcalcium-bindingadaptermolecule1(Iba1),amakerofmicrogliacells,wasperformed,withquantitativereal-timepolymerasechainreaction(qRT-PCR)foritsmRNAquantification.Moreover,Sirt1mRNAandproteinexpressionintheretinasweredetectedbyWesternblotandqRT-PCR.RESULTS:HRSpreservedtheretinalfunctionandmitigatedthereductionofphotoreceptordegenerationinMNU-treatedretinas.ThepresenceofmicrogliacellswassomewhatmoreobviousinHgroupthanthatinMgroupatd1.HRSsuppressedthefurtheractivationofmicrogliacells,withthenumberofmicrogliacellslessthanthatofMgroupatd3.ResultsofqRT-PCRofIba1wereconsistentwiththoseofimmunofluorescencestaining,withthemRNAexpressionofIba1inHgroupmoreintensivethanthatofMgroupatd1(P<0.05),whilelessthanthatofMgroupatd3(P<0.05).Furthermore,theSirt1mRNAandproteinexpressiondecreasedafterMNUadministration,whileHRSmitigatedtheMNU-induceddownregulationofSirt1.CONCLUSION:HRScaneffectivelykeepmicrogliaactivationinducedbyMNUtoanappropriateextent,whileupregulateSirt1inMNU-inducedRP.
简介:QUALITATIVESTUDYOFSIALOMUCINSCHANGESDURINGN-METHYL-N-NITROSOUREA-INDUCEDCOLONICCARCINOGENESISINMICEWangQiang王强;WangYuanhe王元和;...
简介:AbstractObjective:The prevalence of midline birth defects, such as gastroschisis, has increased worldwide, over the last few decades. This study aims to explore the prevalence, maternal epidemiological characteristics, and natural history of neonates affected by gastroschisis at the University Hospital of León city, Nicaragua.Methods:Data were collected from the birth defect surveillance system of the Hospital Oscar Danilo Rosales (HEODRA). The analysis included all pregnancies that had gastroschisis complications between January 1 and December 31, 2020. The prevalence of gastroschisis was calculated according to maternal age. The mothers were interviewed, and the clinical records of the newborns were reviewed.Results:Among the 4,460 deliveries included in this study, four cases of gastroschisis were identified, including three live births and one stillbirth. The gastroschisis rate was 8.9 per 10,000 live births (95% confidence interval [CI]: 0.18-17.8). The prevalence among mothers younger than 20 years and those older than 20 years was 26.4 (95% CI: -3.43 to 56.25) and 3.01 (95% CI: 2.89-8.90)/10,000 births, respectively. Mothers of gastroschisis-affected fetuses were of rural origin (n = 3), had normal body mass indexes (n = 3), were exposed to tobacco and wood smoke (n = 2), and one was exposed to pesticides during the periconceptional period. Primary closure of the gastroschisis was performed on one patient, and complex gastroschisis for intestinal perforation was observed in another patient. The mean hospitalization duration was 33 days, and two patients were discharged alive.Conclusions:Gastroschisis was a significant birth defect among children delivered at HEODRA in 2020. Its prevalence in Nicaragua was higher than that in other countries in the region. All complicated pregnancies were young women with unplanned pregnancies, from rural areas, with exposure to secondhand smoke, and without vitamin supplements before or during the first trimester of pregnancy. Only 67% of infants survived after hospital discharge.
简介:摘要 目的:了解临床N0-N2级护士对生存质量的认知状况及其影响因素,从而促进护理质量的提高并起到推动优质护理服务的作用。方法:采用自设问卷,对我院和某三甲医院心血管内科、呼吸科、肾内科、消化内科、神经内科、肿瘤科、内分泌科、脑外科、肝胆外科和骨伤科护士进行问卷调查,内容包括一般资料、护士对生存质量的认知和态度以及领床护士开展评价生命质量的困难等。结果:①30.0%的护士知道生命质量,52.8%的不知道生存质量的概念,并且有58.6%的不知道目前国内生命质量的研究方向。②在临床上有81.4%从没有运用过生命质量。③56.5%很愿意进一步了解学习生命质量,并且有51.0%希望通过专题讲座的方式来学习。
简介:由乙酰苯胺经Vilsmeier反应步骤制备对甲酰基乙酰苯胺时,以86%收率意外得到N,N-二甲氨基甲基苯亚胺.产物结构经MS,IR1HNMR,13CNMR光谱及其盐酸盐和硫酸盐的C、H、N、元素分析给以确正.此法为N,N-二甲基脒类化合物提供了新的合成途径.