简介：[ 摘要 ] 幼儿活动室凌乱不堪，区域材料摆放不整洁；墙上的物品损坏后，没有及时修补；学具柜面上、教师办公桌上堆满了各种各样的材料，活动室里的各个角落经常摆放着环境布置剩下的物品，想找件东西都觉得非常困难。相信这些常见的现象，每个幼儿园都存在。而这些现象极大地降低了教师的工作效率和质量，影响了教师日常工作的顺利开展，对幼儿良好行为习惯的培养也是有极大的影响。因此 ,在幼儿园推行 6S管理是非常重要的。

简介：摘要：“群文教学”和大单元背景下的“语文学习任务群”等概念的推行，使语文教学的过程性越来越向系统性、科学性靠拢。那么作为教学的最后一环——语文作业，也应当紧随改革的大潮，由散碎、组合向系统性、科学性靠拢，注重培养学生的语文核心素养。建立作业系统，让作业和课堂紧密衔接名，很显然在当下的教学活动，对于保障教学的完整性，对于学生核心素养的培养，是由重大意义的。

简介：【摘要】单元整体教学是一种以主题单元为教学内容，整体设计、组织教学活动的教学模式，集整体性、有序性、综合性为一体，广泛应用于各学科教学。在小学英语课堂教学中，单元整体教学有利于促进学生能力的发展。教师应根据小学英语教材的编写体系，站在单元整体的高度，准确把握教材各版块之间之间的联系，统筹各课时的教学，最大限度地促进学生英语语言能力的发展。

简介：[摘要]在设计工作中有时会遇到建筑物基础不在同一标高，存在高差，且工程要求先施工较浅基础，经过计算对建筑物之间的距离提出最小要求成为方案设计时结构工程师的必要工作，本文结合工程实例提出最小距离的确定过程。

简介：AbstractBackground:Epithelial to mesenchymal transition (EMT) is a key process in determining distant metastasis and intra-hepatic dissemination of hepatocellular carcinoma (HCC). Follistatin (FST) family members are considered to be an attractive therapeutic targets and prognostic indicators in cancers. As a derivative of FST, Follistatin Like 5 (FSTL5) may play a similar role in HCC cells. This study aimed to investigate the expression and function of FSTL5 in HCC and its role in EMT.Methods:FSTL5, E-cadherin and vimentin in HCC, and paracancerous tissues were detected by immunohistochemistry. Correlation of FSTL5 expression with overall survival was assessed. The proliferation and invasion of HCC cell lines SK-Hep1 and MHCC-LM3 were analyzed by cell counting kit-8 and Transwell assays. The expression of FSTL5, E-cadherin, and vimentin in HCC cells was examined by polymerase chain reaction and Western blot analysis. T-test was used to analyze the difference in proliferation and invasion ability between groups. The Spearman rank correlation test was used to detect the correlation between the expression of FSTL5 and E-cadherin or vimentin.Results:The expression of FSTL5 in HCC was lower than that in paracancerous tissues (9.97% vs. 82.55%, χ2 = 340.15, P < 0.001). Patients with high FSTL5 expression had a better prognosis (χ2= 8.22, P= 0.004) and smaller tumor diameter (χ2 = 45.52, P < 0.001), less lymph node metastasis (χ2 = 5.58, P= 0.02), earlier tumor node metastasis stage (χ2 = 11.29, P= 0.001), a reduced number of tumors (χ2 = 5.05, P= 0.02), lower alpha-fetoprotein value (χ2 = 24.36, P < 0.001), more probability of hepatitis carrying (χ2 = 40.9, P < 0.001), and better liver function grade (χ2 = 5.21, P = 0.02). Immunohistochemistry showed that FSTL5 expression in HCC tissues was positively correlated with E-cadherin expression (r = 0.38, P < 0.001) and negatively correlated with vimentin expression (r = -0.385, P < 0.001). Furthermore, over-expression of FSTL5 up-regulated the expression of E-cadherin and down-regulated the expression of vimentin in SK-Hep1 (negative control [NC] vs. FSTL5-interfering group [Lv-FSTL5]: E-cadherin [t= 45.03, P < 0.001], vimentin [t= 67, P < 0.001]) and MHCC-LM3 (NC vs. Lv-FSTL5: E-cadherin [t = 50, P < 0.001], vimentin [t = 72.75, P < 0.001]) cells at mRNA level. The same as protein level. In addition, the over-expression of FSTL5 inhibited the proliferation (NC vs. Lv-FSTL5: SK-Hep1, 3 d [t = 7.324, P = 0.018], 4 d [t = 6.23, P = 0.021], 5 d [t= 10.21, P= 0.003]; MHCC-LM3, 3 d [t= 4.32, P= 0.037], 4 d [t= 7.49, P= 0.012], 5 d [t= 9.3661, P = 0.009]) and invasion (NC vs. Lv-FSTL5: SK-Hep1, t= 21.57, P < 0.001; MHCC-LM3, t= 18.04, P < 0.001) of HCC cells.Conclusions:Down-regulation of FSTL5 may contribute to EMT of HCC, and FSTL5 is a potential target in the treatment of HCC.

简介：摘要目的调查四川省自贡市6个月～6岁儿童缺铁性贫血的状况。方法测定2017年5月至2019年11月在自贡市妇幼保健院儿童保健门诊进行健康体检的2 092例6个月～6岁儿童的血红蛋白值，分析儿童缺铁性贫血的影响因素。结果缺铁性贫血检出率为7.55%，以轻度贫血为主；男童、女童缺铁性贫血检出率比较差异未见统计学意义(P>0.05)。6～12个月组儿童缺铁性贫血检出率最高，不同年龄组间缺铁性贫血检出率比较差异有统计学意义(P<0.05)。母亲孕期贫血、母亲孕期未合理补铁、低出生体质量、早产、母亲文化程度低及未合理添加辅食是儿童缺铁性贫血的相关因素。结论儿童定期健康检查非常重要，应早期发现、早期诊断、早期治疗缺铁性贫血，以降低贫血对儿童健康的危害。

简介：【摘要】近些年来，幼儿事故发生得越来越频繁，这些悲剧使我们警觉，国家、社会、幼儿园和家庭对于幼儿的安全教育力度和广度都远远不够，安全教育迫在眉睫。本论文采用了文献综述法和访谈法对马兰幼儿园的3届大班幼儿掌握得安全知识进行了调查。调查结果表明大班幼儿掌握的安全知识现状不是特别好，不但幼儿个体之间差异性很大，而且安全教育的内容以偏概全，导致幼儿对某些方面的安全知识掌握得很好，而某些方面几乎一无所知。

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简介：摘要葡萄糖-6-磷酸脱氢酶(G6PD)是磷酸戊糖途径的关键酶，参与体内多种代谢过程及氧化还原平衡。近年来研究发现，G6PD在多种肿瘤组织中活性升高，其可调控肿瘤细胞的增殖和凋亡、血管生成、远处转移及放化疗抵抗等，在肿瘤发生发展中起着重要作用，G6PD有望成为新兴的肿瘤治疗靶点。

简介：【摘要】目的：分析0～6岁儿童保健的举措，分析具体的结果。方法：选择2017年01月-2018年12月我院接受的0-6岁的80例儿童为对象，随机实施分组，对照组40例不采用任何干预举措，观察组的40例实施的是保健干预指导，保健指导后对具体的数据分析，总结效果。结果：对比两组儿童的自身发育情况，观察组的PDI、MDI指标值高于对照组。观察组的疾病发生几率是5%，对照组是12.5%，观察组的疾病发生几率低于对照组，数据对比具备统计学意义（P＜0.05）。结论：针对0-6岁的儿童实施保健干预指导，整体上有重要的作用，需要及时落实，确保儿童的身心健康发展。

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简介：摘要：随着新课程改革制度的不断深化，在当前的课堂教学中教师更加注重自身教学方式的调整。核心素养对于学科教学来说是一个较为关键的因素，以核心素养为背景进行的小学英语单元整体教学设计能够促进班级学生在课堂中有更为出色的表现；以单元为整体深度挖掘教材内容，开展课堂教学，确定英语课堂学习的目标和任务，促进学生在课堂上对于英语知识有一个深刻的理解。因此在本文中笔者结合自身的教学经验，探讨一下如何以核心素养为背景开展小学英语单元整体教学设计研究。

简介：AbstractBackground:Pancreatic cancer (PC) is a highly deadly malignancy with few effective therapies. We aimed to unmask the role that long non-coding RNA small nucleolar RNA host gene 6 (SNHG6) plays in PC cells by targeting far upstream element binding protein 1 (FUBP1) via microRNA-26a-5p (miR-26a-5p).Methods:SNHG6 expression was predicted by bioinformatics, followed by verification via reverse transcription quantitative polymerase chain reaction. Then, the interactions among SNHG6, miR-26a-5p, and FUBP1 were detected through online software analysis, dual luciferase reporter assay and RNA pull-down. After that, cells were treated with different small interfering RNAs and/or mimic to determine the interactions among SNHG6, miR-26a-5p, and FUBP1 and their roles in PC cells. Finally, the role of SNHG6 in tumor growth in vivo was evaluated by measuring the growth and weight of transplanted tumors in nude mice. A t-test, one-way and two-way analysis of variance were used for data analysis.Results:Compared with that in normal tissues, SNHG6 was highly expressed in PC tissues (1.00 ± 0.05 vs. 1.56 ± 0.06, t= 16.03, P < 0.001). Compared with that in human pancreatic duct epithelial cells (HPDE6-C7), SNHG6 showed the highest expression in PANC-1 cells (1.00 ± 0.06 vs. 3.87 ± 0.13, t= 34.72, P < 0.001) and the lowest expression in human pancreatic cancer cells (MIAPaCa-2) (1.00 ± 0.06 vs. 1.41 ± 0.07, t= 7.70, P= 0.0015). Compared with the levels in the si-negative control group, SNHG6 (0.97 ± 0.05 vs. 0.21 ± 0.06, t = 16.85, P < 0.001), N-cadherin (0.74 ± 0.05 vs. 0.41 ± 0.04, t= 8.93, P < 0.001), Vimentin (0.55 ± 0.04 vs. 0.25 ± 0.03, t= 10.39, P < 0.001), and β-catenin (0.62 ± 0.05 vs . 0.32 ± 0.03, t= 8.91, P < 0.001) were decreased, while E-cadherin (0.65 ± 0.06 vs. 1.36 ± 0.07, t= 13.34, P < 0.001) was increased after SNHG6 knockdown or miR-26a-5p overexpression, accompanied by inhibited cell proliferation, migration, and invasion. SNHG6 overexpression exerted the opposite effects. SNHG6 upregulated FUBP1 expression by sponging miR-26a-5p. Silencing SNHG6 blocked the growth of PC in vivo.Conclusion:Silencing SNHG6 might ameliorate PC through inhibition of FUBP1 by sponging miR-26a-5p, thus providing further supporting evidence for its use in PC treatment.

简介：摘要目的探讨CK5/6、DSG3、P40、TTF-1、CK7、NapsinA在非小细胞肺癌(NSCLC)活检小标本中鳞状细胞癌(SCC)与腺癌(AC)的表达及其鉴别意义。方法采用免疫组化SP法检测2016年1月至2017年12月在滕州市中心人民医院住院治疗的非小细胞肺癌(NSCLC)120例活检小标本中CK5/6、DSG3、P40、TTF-1、CK7、NapsinA表达，并结合NSCLC的临床特征进行分析。结果CK5/6、DSG3和P40在肺鳞状细胞癌(SCC)组织中的表达率分别为100.0%(56/56)、89.3%(50/56)和96.4%(54/56)，特异度分别为90.6%、100.0%和100.0%；NapsinA、TTF-1、CK7在肺腺癌(AC)组织中的表达率分别为81.3%(52/64)、90.6%(58/64)和93.8%(60/64)，特异度分别为100.0%、92.9%和96.4%。CK5/6、DSG3和P40在SCC组织中的表达与在AC组织中的表达差异有统计学意义(P<0.05)，NapsinA、TTF-1、CK7在AC组织中的表达较SCC组织中的表达差异有统计学意义(P<0.05)。结论CK5/6、DSG3、P40、TTF-1、CK7、NapsinA在非小细胞肺癌活检小标本SCC与AC的鉴别诊断中具有重要意义。

简介：摘要： 复习课作为小学数学三种课型之一，在教学中具有重要的地位。目前教学现状是以老师为主导，课堂上老师引导学生回顾知识，再老师出题让学生练习，很大程度上把复习课上成练习课的感觉。为了改变现状，发挥学生的自主性，让学生能主动地、有选择性地参与单元复习。笔者从以下四个方面进行了改变：一、自主整理知识，形成知识网络； 二、 自主分析错误，弥补知识漏洞；三、自主选遍练习，提升知识运用；四、自主选择评价，改善导向功能。

简介：摘要：五星教学模式是当代著名教育技术理论家梅瑞尔提倡的教学理论，其核心是在聚焦问题的宗旨下，通过循环激活旧知、示证新知、应用新知、融会贯通四个教学环节，循序渐进地提升学生的学习兴趣，帮助学生熟练掌握相应的知识技能，能够有效改善目前课堂教学中只重视信息呈现、不注重有效教学的弊端。本文以《Web前端开发技术》中的“HTML5的表单元素”为例，对五星教学模式在混合式课堂中的实际应用进行了探讨。

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简介：摘 要：构建基于标准的单元整体教学活动是我们提升初中数学授课质量的有效方法。要想教好初中数学知识，我们就需要尊重数学学科特点，立足课程标准、立足单元整体，优化数学课堂。

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