简介：ThepresentstudyinvestigatedthechemicalcompositionofethylacetateextractsfromanendophyticactinomyceteStreptomycessp.A0916anditshostPolygonumcuspidatum.Acomparativeanalysisoftheantimicrobialandantioxidantpropertiesoftheextractswasalsoconducted.32compoundsofP.cuspidatumand23compoundsofStreptomycessp.A0916wereisolatedandidentifiedbyGC/MS.Antimicrobialactivitiesoftheextractswereevaluatedusingeightmicrobialstrains(3Gram-positivebacteria,3Gram-negativebacteria,and2fungi).TheStreptomycessp.A0916extractsshowedawiderangeofantimicrobialactivitiesandpresentedgreaterantimicrobialeffectivenessthantheP.cuspidatumextracts.Theminimuminhibitoryconcentration(MIC)ofStreptomycessp.A0916extractsagainsttheampicillin-resistantstrainEnterococcusfaeciumSIIA843was32μg·mL-1.Furthermore,theextractshadgreaterantimicrobialeffectagainstGram-positivebacteriathanGram-negativebacteria.Finally,theantioxidantactivityoftheStreptomycessp.A0916extractswasequaltothatoftheP.cuspidatumextracts.Inconclusion,ourresultssuggestthattheendophyticactinomycetesofthemedicinalplantsareanimportantsourceofbioactivesubstances.

简介：无

简介：Pomegranateleaf(PGL)hasadefiniteroleinregulatinglipidmetabolism.However,pharmacokineticresultsshowthemainactiveingredient,ellagicacid,inPGLhasloweroralbioavailability,suggestingthatthelipid-loweringeffectofPGLmayactthroughinhibitinglipidabsorptioninthesmallintestine.Ourresultsdemonstratedthatpomegranateleafanditsmainactiveingredients(i.e.,ellagicacid,gallicacid,pyrogallicacidandtannicacid)werecapableofinhibitingpancreaticlipaseactivityinvitro.Incomputationalmoleculardocking,thefouringredientshadgoodaffinityforpancreaticlipase.AcutelipidoverloadexperimentsshowedthatalargedosageofPGLsignificantlyreducedserumtotalcholesterol(TG)andtriglycerides(TC)levelsinadditiontoinhibitingintestinallipaseactivity,whichdemonstratedthatPGLcouldinhibitlipaseactivityandreducetheabsorptionoflipids.WealsofoundthatPGLcouldreversethereducedtight-junctionproteinexpressionduetointestinallipidoverload,promoteOccludinandClaudin4expressioninthesmallintestine,andenhancetheintestinalmucosalbarrier.Inconclusion,wedemonstratedthatPGLcaninhibitlipidabsorptionandreducebloodTGandTCbytargetingpancreaticlipase,promotingtight-junctionproteinexpressionandtherebypreventingintestinalmucosadamagefromanoverloadoflipidsintheintestine.

简介：

简介：瞄准validatory屏蔽puffer鱼以puffer鱼皮肤和肝提取的Arothronimmaculatus为抗菌剂试金被使遭到的如此的一个方法被做的抗菌剂。屏蔽试金的方法抗菌剂用标准圆盘散开方法在十连续人的病原的细菌、真菌的病原体被做。结果证实了的结果对大多数病原体的阳性的测试使用了。对在肝摘录的2.5公里和对在皮肤摘录的Vibrio霍乱的抗菌剂效果的9.8公里的Staphyloccocusaureus的最大的抗菌剂效果被报导。没有抗真菌的效果。这个实验证实那条puffer鱼的结论是抗菌剂力量的来源。

简介：试图学习在里面vivo男白化体老鼠上的Tabernaemontanadivaricata叶摘录的反煽动性的活动。方法水的煎和甲醇叶摘录为他们减少巴豆的能力被测试在在热门申请以后的鼠标耳朵的导致油的浮肿。甲醇叶摘录dose-dependently禁止了巴豆在老鼠的导致油的耳朵浮肿(ID50<500?g

简介：Theanticoagulanteffectofleechsalivawastraditionallyemployedinthetreatmentofdiabetesmellituscomplicationssuchasperipheralvascularcomplications.Thisstudywascarriedouttoexaminetheeffectofleechsalivaextract(LSE)onbloodglucoselevelsinalloxan-induceddiabeticrats.First,LSEwascollectedfromleecheswhichwerefedonaphagostimulatorysolution.Second,totalproteinconcentrationwasestimatedusingtheBradfordassay.Third,diabeticratswereinjectedsubcutaneously(sc)withLSEatdosesof500and1000μg·kg1bodyweight(bw).Otherdiabeticratswereinjectedscwithinsulinatdosesof10and20U·kg1bw.AnothergroupwasinjectedsimultaneouslywithLSE(250μg·kg1bw)andinsulin(10U·kg1bw).Fastingbloodglucose(FBG)concentrationsweremonitoredduringastudyperiodofeighthoursatregularintervals.FindingsshowedthatbothdosesofLSEresultedinasignificantandgradualdecreaseinFBGstartingfrom10%18%downfallaftertwohoursofinjectionreachingthemaximalreductionactivityof58%aftereighthours.Remarkably,LSEwassufficienttobringtheratstoanearnorm-glycemicstate.Thehighdoseofinsulininducedaseverehypoglycemicconditionafter24hofinjection.ThelowerdosewasabletodeclineFBGfor26hinratswhichbecamediabeticagainafter8h.Ontheotherhand,theconcurrentinjectionoflowdosesofLSEandinsulinproducedahypoglycemiceffectwithallratsshowingnormalFBGlevels.Takentogether,thesefindingsindicatedthatthesubcutaneousinjectionofLSEofthemedicinalMalaysianleechwasabletoprovidebetterglycemiccontrolcomparedwithinsulin.Moreover,thesynergismbetweenLSEandinsulinsuggeststhatLSEcouldbeutilizedasanadjuvantmedicationinordertoreduceinsulindosageortoachievebettercontrolofbloodglucose.

简介：CheonggukjangisasoybeanpastemadebyfermentingwholecookedsoybeanswithBacillussubtilis.Cheonggukjangcontainsafibrinolyticenzymethatcouldprovideclinicalapplicationsforremovingbloodclots.Inthepresentstudy,theterm'cheonggukjangkinase'(CGK)wasusedtorefertothisfibrinolyticenzyme.ThethrombolyticeffectsofCGKwereanalyzedinaratmodelofcerebralembolicstrokeproducedbymiddlecerebralarteryocclusion(MCAO).Resultsfromfibrinandplatelet-richclotlysisassaysdemonstratedthatthrombolyticactivitywasgreatestinCGKs,whichwereculturedfor40hours.Inaddition,T50,thetimeneededtodecompose50%oftheclot,didnotchangewithplasminogentreatment,indicatingthatCGKwasnotaplasminogenactivator,butwasratherpresumedtoactasaplasmin-likeprotein.AnintravenousinfusionofCGK(1Uplasmin-likeactivity/100μgCGK/kg)at1hourafterMCAOresultedinremovalofclotsinaratmodelofcerebralembolicstroke.CGK-treatedgroupsexhibitedasignificantdose-dependentreductionininfarctvolume.CGKtreatmentalsoimprovedfunctionalrecovery,asassessedbyneurologicaldeficitscores.DecreasedinfarctvolumeandimprovedfunctionalrecoveryfollowingCGKtreatmentwasgreatercomparedwithrecombinanttissueplasminogenactivator(10mg/kg).TheseresultssuggestedthatCGKeffectivelyreducedinfarctvolumeandimprovedfunctionalrecoveryfollowingischemicbraininjury.CGKexhibitsanumberofpotentialclinicalapplicationsinthetreatmentofcerebralembolicstroke.

简介：CD59可以由绑定禁止补充的cytolytic活动到C8/C9并且保护主人房间膜免于相应的膜攻击建筑群(MAC)。然而，CD59是广泛地肿瘤房间上的overexpressed，它在tumorigenesis被含有。相对MAC的CD59的活跃地点仍然被糊涂。是报导MAC有约束力的地点在蛋白质的远侧的脸在残余W40附近集中了的膜上位于一条恐水病的沟的附近。这里，二指导地点的mutagenesis被重叠延期PCR执行删除残余W40地点(异种1，M1)或把C39W40K41改变到W39W40W41(异种2，M2)。然后，我们构造了变异的CD59真核细胞的表达式系统并且与野类型的CD59相比在CHO房间上调查了他们的生物函数。表示recombinant蛋白质的CHO房间的稳定的人口被immunotechnique屏蔽。在30段落culturing以后，蛋白质能被测试。染料版本试金建议M1CD59对补充失去这项活动，当M2CD59稍微增加反补充活动时。结果显示人的CD59的W40对它的活动重要，并且这个地点的禁止可以是增加补充活动并且治疗肿瘤的一个潜在的方法。

简介：目的：通过蛋白质组技术鉴定针刺抗哮喘血清中差异蛋白，明确针剌抗哮喘效应的物质基础。方法：采用蛋白质双向电泳技术，对针刺抗哮喘血清进行蛋白质组份差异分析，并对差异蛋白质点进行质谱鉴定。结果：双向电泳显示，与其它组相比，肾上腺切除哮喘模型针刺组血清与哮喘模型针刺组血清有一个明显深染的蛋白点（MW=44．1kD，PI=5．1），质谱结果为60S核糖体蛋白L13或假想蛋白FLJ32191；双向电泳显示，与其它组相比，肾上腺切除哮喘模型针刺组血清有两个高峰度蛋白点（MW=39．8kD，PI=4．9和MW=39．5kD，PI=5．0），质谱分析表明依次为锌指蛋白208、甲状腺激素受体相关蛋白；双向电泳显示，肾上腺切除哮喘模型针刺组血清中有两个明显区别于其他组的特有蛋白点（MW=17．3kD，PI〈4．5和16．9kD，PI〈4．5）。质谱分析表明依次为亲环蛋白A、锌指蛋白91。结论：针刺治疗哮喘，是整体调整的多靶点效应，与免疫调节、基因表达、蛋白质合成等密切相关。针刺应答蛋白质功能有待深入研究。

简介：Pleurotussajorcaju(P。sajorcaju)，一朵可食、无毒的蘑菇，作为抗氧化剂被评估，antitumor，反煽动性并且antihypertensive活动。P。sajorcaju是糖类，饮食的纤维，必要氨基酸，矿物质，维生素B，folic酸和类固醇的好来源。反煽动性，immunomodulatory和止痛活动水并且P的菌丝体的methanolic摘录。sajorcaju被调查(数据没被显示出)。这发现建议那P提取。sajorcaju能对煽动性、自体免疫的疾病被使用。那么，P。为它的antiarthritic活动检验的sajorcaju。植物与水和甲醇镇定、独立提取。为antiarthritic活动500和1000mg

简介：AbstractBackground:Fibroblast-like synoviocytes (FLSs), resident mesenchymal cells of synovial joints, play an important role in the pathogenesis of rheumatoid arthritis (RA). Dickkopf-1 (DKK-1) has been proposed to be a master regulator of bone remodeling in inflammatory arthritis. Here, potential impairation on the activity of FLSs derived from RA to small interfering RNAs (siRNAs) targeting DKK-1 was investigated.Methods:siRNAs targeting DKK-1 were transfected into FLSs of patients with RA. Interleukin (IL)-1β, IL-6, IL-8, matrix metalloproteinase (MMP) 2, MMP3, MMP9, transforming growth factor (TGF)-β1, TGF-β2 and monocyte chemoattractant protein (MCP)-1 levels in the cell culture supernatant were detected by enzyme-linked immunosorbent assay (ELISA). Invasion assay and 3H incorporation assay were utilized to investigate the effects of siRNAs targeting DKK-1 on FLSs invasion and cell proliferation, respectively. Western blotting was performed to analyze the expression of nuclear factor (NF)-κB, interleukin-1 receptor-associated kinase (IRAK)1, extracellular regulated protein kinases (ERK)1, Jun N-terminal kinase (JNK) and β-catenin in FLSs.Results:DKK-1 targeting siRNAs inhibited the expression of DKK-1 in FLSs (P < 0.01). siRNAs induced a significant reduction of the levels of IL-6, IL-8, MMP2, MMP3 and MMP9 in FLSs compared to the control group (P < 0.05). DKK-1 targeting siRNAs inhibited the proliferation and invasion of FLSs (P < 0.05). Important molecules of pro-inflammatory signaling in FLSs, including IRAK1 and ERK1, were decreased by the inhibition of DKK-1 in FLSs. In contrast, β-catenin, a pivotal downstream molecule of the Wnt signaling pathway was increased.Conclusions:By inhibiting DKK-1, we were able to inhibit the proliferation, invasion and pro-inflammatory cytokine secretion of FLSs derived from RA, which was mediated by the ERK or the IRAK-1 signaling pathway. These data indicate the application of DKK-1 silencing could be a potential therapeutic approach to RA.

简介：BACKGROUND:ThepharmacologicalactionoftraditionalChinesemedicinecompoundisthecomprehensiveeffectofthevariousingredients,andtheinteractionsofvariousingredientsarecloselycorrelatedwiththefinaleffect.InordertorevealthecompatibilitymechanismofBHD'sprescriptionintreatingandpreventingischemiccerebrovasculardisease,weneededexploretheeffectandrelationofingredientsintheprescription.OBJECTIVE:ToobservetheeffectofBuyangHuanwudecoction(BHD)andAstragalusmongholicusontheactivityofplateletactivatingfactorreceptor(PAFR)intheplateletofrabbitsinvitro,andinvestigatethemechanismofAstragalusmongholicus.DESIGN:Adecomposedrecipesstudy.SETTING:GuangzhouUniversityofTraditionalChineseMedicine.MATERIALS:FiveNewZealandrabbits,weighing2-3kg,bothsexes,wereused.BHDwascomposedofShengHuangQi120g,DangGuiWei6g,ChiShao4.5g,ChuanXiong3g,DiLong3g,TaoRen3g,HongHua3g.TheprescriptionforactivatingbloodcirculationconsistedofDangGuiWei6g,ChiShao4.5g,ChuanXiong3g,DiLong3g,TaoRen3gandHongHua3g.Theprescriptionforinvigoratingqiconsistedof120gShengHuangQi.ThepreparedherbalpieceswerepurchasedfromthetraditionalChinesemedicineDispensaryofFoshanSecondPeople'sHospital,andappraisedbyProfessorXufromScienceofChineseMateriaMedicaCollege,GuangzhouUniversityofTraditionalChineseMedicine.3H-PAFwassuppliedbyAmershamCo.,Ltd.(specificactivity:6.475TBq/mmol;batchnumber:200402);PAFstandardbyBiomolCo.,Ltd.(batchnumber:P1318V).METHODS:TheexperimentswerecarriedoutintheLaboratoryofNuclearMedicine,GuangzhouUniversityofTraditionalChineseMedicinefromSeptembertoDecember2004.①InjectionsofBHD,prescriptionsforactivatingbloodcirculationandinvigoratingqiwerepreparedbythedecoctionandalcoholsedimentationtechnique.Rabbitcommoncarotidarteryblood(40mL)wasdrawnviaintubationtoprepareplateletsuspensionof(0.8-1.0)×1010L-1.②Determinationo

简介：Objective:ToexplorethemechanismofEcdysterone(ECR)inpreventionoflearningandmemorydysfunctionoftheratsinducedbyβ-amyloidpeptide(Aβ25-35).Methods:NinetyWistarmaleratswererandomlydividedintofivegroups,thecontrolgroup,themodelgroup,thetreatedgroups(ECR4mg·kg^-1andECR8mg·kg-1andNimodipine

简介：试图从粗略的摘录和从东北泰国收集的Phellinus蘑菇的十种的粗略的部分调查抗氧化剂活动和全部的酉分的内容。样品向2为他们的激进的清除活动被测试的方法，2-diphenyl-1-pricylhydrazyl(DPPH)激进分子(DPPH方法)和全部的酉分的内容(Folin-Ciocalteu方法)。一些调查摘录展出了有势力基清除的结果有从的IC50的活动(7.30来慲整?楷桴琠敨?湩漠摲牥琠?湩牣慥敳琠敨物瀠潲畤瑣癩瑩y

简介：

简介：Thepurposeofthissubjectwastoinvestigatemolecularepidemiologyofoxacillin-resistantStaphylococcusaureus(MRSA)isolatedfromhospitalizedpatients,andtosurveytheinvitroactivityofteicoplanin,vancomycinandother9antibioticsagainstStaphylococcusspecies.MRSAweredetectedbyoxacillin-NaCl-containingscreenagar.ThehomologyofnosocomialMRSAfromICUandRCUwasdeterminedbypulse-fieldgelelectrophoresis.Agardiffusion,EtestandagardilutionwereusedtocomparetheinvitroactivityofteicoplaninandvancomycinagainstStaphylococcussppfrom2001to2003atPekingUnionMedicalCollegeHospital.WHONET-5.3softwarewasusedtoanalyzetheantimicrobialsusceptibilitydata.From2001to2003,theprevalencesofMRSAwere56.5%,65.3%,64.7%,respectively.PFGEfoundmostofMRSAfromICUandRCUwerecloselyrelated.AllofS.aureusandS.epidimidisisolatesweresusceptibletoteicoplaninandvancomycinfrom2001to2003.However,1isolateofS.haemolyticuswasresistantand9isolatesintermediatetoteicoplanin.Theminimalinhibitoryconcentrationofteicoplanindidnotcorrelatewellwithzonediameter,wheninoculumincreasedby100folds,thezonediametersofteicoplanindecreasedmoregreatlythanthoseofvancomycin.In2002,severeoutbreakscausedbyMRSAstrainshadbeenfoundinICUandRCUwards.TeicoplaninandvancomycinhadgoodactivityagainstclinicalisolatesofStaphylococcispp.TeicoplaninwaslessactivethanvancomycinagainstS.haemolyticus.MostofS.haemolyticusisolateswereintermediatetoteicoplanin.Antimicrobialsusceptibilitytestingofteicoplaninwasinfluencedbythediffusionspeedintheagarandinoculumsize.

简介：BACKGROUND:RecentstudieshavesuggestedthatmitochondrialATP-sensitiveK+channelopenerscouldreducemyocardiuminfarctsize,andprotectthefunctionofthemitochondria.OBJECTIVE:ToinvestigatethechangesofcerebralinfarctionvolumeandtheactivityofmarkerenzymesinbrainmitochondriaofratsgiventheATP-sensitiveK+channelopener,nicorandil,beforefocalcerebralischemia/reperfusion(I/R).DESIGN,TIMEANDSETTING:Randomized,controlledanimalexperiment,completedattheBrainScientificResearchCenteroftheAffiliatedHospitalofQingdaoUniversityfromJulytoNovember2007.MATERIALS:SixtyhealthymaleWistarratsweighing280–300g.Nicorandil,5-hydroxydecanoate(5-HD)andcytochromeCwerepurchasedfromSigmaintheUSA.Standardmalondialdehyde(MDA)andproteinwerepurchasedfromNanjingJianchengBiotechnologyInstitute.METHODS:Sixtyratswererandomlydividedintoashamoperationgroup,amiddlecerebralarteryocclusion(MCAO)group,anicorandilgroupandanicorandil+5-HDgroup.MCAOfor2hourswasperformedintheMCAOgroup,nicorandilgroupandnicorandil+5-HDgroup.Atotalof5mLsalineweregiventotheMCAOgroupbeforeMCAO.ThenicorandilgroupwasinjectedwiththeATP-sensitiveK+channelopenernicorandil10mg/kgintraperitoneally30minutesbeforeMCAO.Thenicorandil+5-HDgroupwasinjectedwith5-HD10mg/kgintravenously15minutesbeforethesametreatmentasthenicorandilgroup.MAINOUTCOMEMEASURES:Infarctvolumebytotalbrainslicecalculation,activitiesofsuccinatedehydrogenase(SDH)andcytochromeoxidase(CO),andcontentofMDAwereobservedat22hoursofreperfusionafter2hoursMCAO.RESULTS:Sixtyratswereincludedinthefinalanalysis,withoutanyloss.(1)Infarctvolume:comparedwiththeMCAOgroupandnicorandil+5-HDgroup,thepercentageofinfarctvolumewassignificantlydecreasedinthenicorandilgroup(P<0.01).(2)ThecontentofMDA,expressionofSDHandCOinbrain:theexpressionsofSDHandCOintheshamop

简介：

简介：AbstractBackground:Early body composition changes, associated with physical inactivity and disease advancement are devastating for patient-related outcomes in predialysis chronic kidney disease (CKD), thus warranting a detailed analysis of body composition beyond conventional measures.Methods:The study included 40 subjects diagnosed with CKD; recruited between January to May 2021. Body composition was measured using the multifrequency analyzer, InBody 770. International Physical Activity Questionnaire-Short Form was used to assess physical activity. Suitable statistical analyses were performed using SPSS 21.0.Results:The mean age of the subjects was 58.68 ± 12.24 years. Sarcopenic obesity was prevalent in 62.5% of the subjects. Body mass index under identified obesity by 15% compared to percent body fat, especially in subjects with low muscle mass. The decline in a unit of estimated glomerular filtration rate (eGFR) significantly correlated with a decrease in weight (p = 0.02), body fat mass (p = 0.05), visceral fat area (p = 0.05), and phase angle (p = 0.01) with marginal changes in waist–hip ratio and extracellular water/total body water. The effect of physical activity on skeletal muscle mass was homogeneous between low and moderate levels, but significantly different from high activity level.Conclusion:Changes in fat and fluid compartment were associated with eGFR decline, whereas higher physical activity positively affected body composition.