简介：一个实验被进行用荧光灯的微分显示器(软式磁碟机)方法在干旱应激和正常条件下面在米饭叶子和根比较信使rna表达式差别。一积极碎片被H.A的联合孤立黄页(contained0.1%H.A。黄)分离和宏数组屏蔽方法。比作ArabidopsisthalianaNADPH氧化还原酶基因，它有96%身份。cDNA是1423bp，并且包含了与345氨基酸残余编码蛋白质的1048bp的一个完全的开的读物框架。而且，基因表示水平在正常条件下面比那在干旱应激下面是更高的。在干旱反应下面的NADPH氧化还原酶基因的可能的角色也被讨论。

简介：Interleukin-4isacytokineproducedbyactivatedTcells,mastcells,andbasophilsthatelicitsmanyimportantbiologicalresponses[1](seeTab1).TheseresponsesrangefromtheregulationofhelperTcelldifferentiation[2]andtheproductionofIgE[3]totheregulationoftheadhesivepropertiesofendothelialcellsviaVCAM-1[4],Inkeepingwiththesediversebiologicaleffects,high-affinitybindingsitesforIL-4(Kd20to300pM)havebeendetectedonmanyhematopoieticandnon-hematopoieticcelltypesatlevelsrangingfrom50to5000sitespercell[5].ThisreviewwillfocusonthediscretesignaltransductionpathwaysactivatedbytheIL-4recxeptorandthecoordinationoftheseindividualpathwaysintheregulationofafinalbiologicaloutcome.

简介：·NSGeodeGX1—300处理器3．5寸嵌入式主板，配有LCD，LVDS，视频输出，以太网，音频，CompactFlash，和USB口。

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简介：Objective:Toobservehumanneuronalapoptosissecondarytotraumaticbraininjury,andtoelucidateitsregulativemechanismandthechangeofexpressionofapoptosis-relatedgenes.Methods:Specimensofbrainwerecollectedfromcasesoftraumaticbraininjuryinhumans.Thehistologicalandcellularmorphologywasexaminedbylightandelectronmicroscopy.TheextentofDNAinjurytocorticalneuronswasdetectedbyusingTUNEL.ByinsituhybridisationandimmunohistochemistrythemRNAchangesandproteinexpressionofBcl-2,Bax,p53,andcaspase3p20subunitwereobserved.Results:Apoptoticneuronsappearedfollowingtraumaticbraininjury,peakedat24hoursandlastedfor7days.Innormalbraintissueactivatedcaspase3wasrare,butashorttimeaftertraumaitbecameactivated.Theactivitypeakedat20-28hoursandremainedhigherthannormalfor5-7days.TherewasnoexpressionofBcl-2mRNAandBcl-2proteininnormalbraintissuebut8hoursafterinjurytheirexpressionbecameevidentandthenincreased,peakedat2-3daysandremainedhigherthannormalfor5-7days.TheprimaryexpressionofBax-mRNAandBaxproteinwashighinnormalbraintissue.At20-28hourstheyincreasedandremainedhighfor2-3days;onthe7thdaystheyreturnedtoanormallevel.Innormalbraintissue,p53mRNAandP53wereminimallyexpressed.Increasedexpressionwasdetectedatthe8thhour,anddecreasedat20-28hoursbutstillremainedhigherthannormalonthe5thday.Conclusions:Followingtraumaticinjurytothehumanbrain,apoptoticneuronsappeararoundthefocusoftrauma.ThemRNAandproteinexpressionofBcl-2,Baxandp53andtheactivityofcaspase3enzymeareincreased.

简介：一个研究工作被进行在受影响的健康相关的元素的集中和分发调查变化环境并且在米饭谷物的genotypic差别。XieqingzaoB(indica米饭变化)和Xiushui110(装饰用的梨树米饭变化)的谷物被划分成：壳，糠和milled，基于常规米饭消费和过程。XieqingzaoB在四个不同地点被种，并且赎回地点，它在象Xiushui110的一样的地和季节里被种。另外，另一四indica和四个装饰用的梨树变化在一样的领域和时间里被栽培在milled分析元素。全部的P和phytic酸P的Theaverage集中在糠是最高的，由milled和壳列在后面；Zn，K，Mg，并且因为集中在糠是最高的，当Fe，Ca，和Cu集中在壳是最高的时，由壳和milled列在后面，但是在糠和milled类似。结果显示遗传型和环境显著地影响了所有测试元素的集中，当在谷物的上述元素的分发不在象集中的一样的顺序时。而且，除了97.7%Cu和93.2%ofFe的所有元素平均在壳被扔，也主要在糠(为K和phytic酸P的37.3%and57.7%)或在milled被散布(41.7%，42.6%，40.3%，49.8%为Zn，Mg，同样小孩高山，分别地)。

简介：Inthepresentpaper,theauthorsreviewrecentsituationsofdisordersofthedigestivesystemanditsrelatedresearchprogresses.Inclinicalpractice,Neiguan(PC6)isusuallyselectedasoneofthemainintra-gastricexamination,cesareansection,etc.ConcerningexperimentalindicatedthatNeiguan(PC6)isanimportantandeffectiveacupointfortreatmentofgastrointestinaldisordersinclinic.StimulationofNeiguan(PC6)inducedfavorableregulationofboththeperipheralnervoussystemandcentralnervoussystem,andchangesofthegastrointestinalhormonesecretionmaycontributetoitseffectsintreatingvariousdisorders.

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简介：Thispaperdisccussestheinversescatteringproblemforone-dimensionalSchrodingeroperatorsrelatedtothegeneralStarkeffect.WeprovideaganeralframeworkwhichcanbeappliedbothtotheStark-effectoperatorandtheordinarySchrodingeroperator.

简介：Emodin(1,3,8-trihydroxy-6-methylanthraquinone)couldenhancethesensitivityoftumorcellstoarsenictrioxide(As2O3)-inducedapoptosisviagenerationofROS,butthemolecularmechanismhasnotbeenelucidated.Here,wecarriedoutcDNAmicroarray-basedglobaltranscriptionprofilingofHeLacellsinresponsetoAs2O3/emodincotreatment,comparingwithAs2O3-onlytreatment.Theresultsshowedthattheexpressionofanumberofgeneswassubstantiallyalteredattwotimepoints.Thesegenesareinvolvedindifferentaspectsofcellfunction.Inadditiontoredoxregulationandapoptosis,ROSaffectgenesencodingproteinsassociatedwithcellsignaling,organellefunctions,cellcycle,cytoskeleton,etc.ThesedatasuggestthatbasedonthecytotoxicityofAs2O3,emodinmobilizeeverygenomicresourcethroughwhichtheAs2O3-inducedapoptosisisfacilitated.

简介：Inthepastdecade,usesofantisepticsanddisinfectantsinhospitalsandotherhealthcarecentersarerathercommon,butthechancetodevelopresistancetoantisepticsanddisinfectantsisalsoincreased.Acinetobacterbaumanniiisoneoftheopportunisticbacteriainvolvinginthenosocomialinfection.Inthepresentstudy,thecorrelationoftheantisepticresistanceinA.baumanniiandtheantisepticresistancegeneqacEΔ1wasinvestigatedbymeansofdeterminationofMICs.Meanwhile,theMICsofglutaraldehyde,chlorhexidine,benzalkoniumbromide,iodophorandtrichloroisocyanurateto80clinicalisolatesofA.baumanniiweredetectedbytubedilutionassayandtheresistancegenesintI1andqacEΔ1intheseisolateswereamplifiedbyPCRandverifiedbyDNAsequencer.ItwasfoundthattheMIC50forthese5antisepticstestedwere32,8,8,4and1μg/mlrespectively,andthedetectionratesofintI1andqacEΔ1genewere60.0%and77.6%respectively.Inaddition,55%ofthe80isolatessimultaneouslypossessedbothintllandqacEΔ1gene,andthepercentageofantisepticresistanceofA.baumanniicarringbothgenestobenzalkoniumbromidewerehigherthanthatwithoutthesetwogenes,however,therewasnosignificantdifferencebetweenintllandqacEΔ1gene.Theresultinbactericidalefficiencyassayindicatedthatchlorhexidinecouldstillproducerapidandstrongbactericidaleffectatconcentrationof1MICafter10minexposure.TheseresultssuggestthattheantisepticresistanceofA.baumanniitovariousantisepticsiscorrelatedwiththepresenceoftheantisepticresistancegenesqacEΔ1inbacteria,thuswarningthattheincreaseoftheantisepticresistanceshouldnotbeignoredandtherelativehighconcentrationorprolongedapplicationtimeisrequiredtoachieveasufficientbactericidaleffect.

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简介：ShortinterferingRNA(siRNA)iswidelyusedforstudyingpost-transcriptionalgenesilencingandholdsgreatpromiseasatoolforbothidentifyingfunctionofnovelgenesandvalidatingdrugtargets.TwosiRNAfragments(siRNA-aand-b),whichweredesignedagainstdifferentspecificareasofcodingregionofthesametargetgreenfluorescentprotein(GFP)gene,wereusedtosilenceGFPexpressioninculturedgfptransgeniccellsofrice(OryzasativaL.;OS),cotton(GossypiumhirsutumL.;GH),Fraserfir[Abiesfraseri(Pursh)Poir;AF],andVirginiapine(PinusvirginianaMill.;PV).DifferentialgenesilencingwasobservedinthebombardedtransgeniccellsbetweentwosiRNAs,andtheseresultswereconsistentwiththeinactivationofGFPconfirmedbylaserscanningmicroscopy,Northernblot,andsiRNAanalysisintestedtransgeniccellcultures.ThesedatasuggestthatsiRNA-mediatedgeneinactivationcanbethesiRNAspecificindifferentplantspecies.TheseresultsindicatethatsiRNAisahighlyspecifictoolfortargetedgeneknockdownandforestablishingsiRNA-mediatedgenesilencing,whichcouldbeareliableapproachforlarge-scalescreeningofgenefunctionanddrugtargetvalidation.

简介：Computationalanalysisisessentialfortransformingthemassesofmicroarraydataintoamechanisticunderstandingofcancer.Herewepresentamethodforfindinggenefunctionalmodulesofcancerfrommicroarraydataandhaveappliedittocoloncancer.First,acoloncancergenenetworkandanormalcolontissuegenenetworkwereconstructedusingcorrelationsbetweenthegenes.Thenthemodulesthattendedtohaveahomogeneousfunctionalcompositionwereidentifiedbysplit-tingupthenetwork.Analysisofbothnetworksrevealedthattheyarescale-free.Comparisonofthegenefunctionalmodulesforcoloncancerandnormaltissuesshowedthatthemodules’functionschangedwiththeirstructures.

简介：ObjectiveChronictinnitusisahighlyprevalentconditionandhasbeenhypothesizedtoresultfromaninnatedisturbanceincentralnervousserotonergictransmission.Giventhefrequentcomorbiditywithmajordepressionandanxiety,wearguethatcandidategenesforthesedisordersarelikelytooverlap.Thepresentstudyaddressesthegeneencodingforthe5-HT1Areceptorasaputativeriskfactorfortinnitus.MethodsIn88subjectswithadiagnosisofchronicsubjectivetinnituswhounderwentadetailedneurootologicalexamination,theentire5-HT1AgenewasamplifiedusingoverlappingPCRproducts.Ampliconswerecustomsequencedbidirectionallyandwerescreenedforvariantsinmultiplealignmentsagainstthehumangenomereference.ResultsWeidentifiedasynonymousC>Texchangeatresidue184(Pro)in7/88subjects,butdetectednomissensevariantsinthepopulationunderstudy.Specifically,thefollowingresidueswerefullyconserved:16(Pro),22(Gly),28(Ile),98(Val),220(Arg),267(Val),273(Gly),and418(Asn).DiscussionThepresentdatacountagainstthecausationofchronictinnitusbyachangeinthe5-HT1Areceptor'saminoacidsequence.However,theallelefrequencyforthe184Prominorallele(0.04)reachedtwicethefrequencyreportedincontrolcohortsfromthesameethnicity.Additionalinvestigationsareinvitedtoclarifytheroleofthe5-HT1Apolymorphisminlargersamples,andtocontrolforcomorbidaffectivedisorders.

简介：AIMToexpressandpurifyLipoprotein-associatedphospholipaseA2(Lp-PLA2),andtoestablishascreeningmodelforLp-PLA2inhibitorsthroughtherecombinantLp-PLA2.METHODSThefull-lengthgeneofLp-PLA2wasclonedfromthedifferentiatedTHP-1cellsbyRT-PCRandPCR.TheLp-PLA2genewassubclonedintothePichiaexpressionvectorpPIC9andintroducedasequenceencodingaC-terminalstretchofsixhistidineresiduesatthesametime.TherecombinantplasmidwastransformedintoPichiapastorisGS115byspheroplastingandthegenewasthenintegratedintotheGS115genome.Lp-PLA2wasexpressedintheyeaststrainGS115byinducingwith0.5%methanol.

简介：BiofilmformationplaysamajorroleinthepathogenesisofnosocomialinfectionscausedbyStaphylococcusepidermidis(S.epidermidis).Ithasbeensuggestedthatproteinencodedbythefoe(fibrinogenbindingprotein)geneofS.epidermidisenhancesbacterialadherencetomedicaldevicesandbiofilmformationbybindingtohostfibrinogen(Fg).Inthisstudy,a1.7kbfoegenefragmentwasamplifiedin111of115strainsofS.epidermidischnicalisolatesusingPCR.Contrarytoexpectations,only14strainsshowedmarginallyincreasedadherencetoFg-coatedpolystyrenewellscomparedwithBSAcoatedwells.Quantitativereal-timePCRrevealednostatisticallysignificantdifferenceinFbeexpressionbetweenFgbindingstrainsandFgnon-bindingstrains.Fttahermore,inthepresenceofsolubleFg,S.epidermdisbiofilmformationdecreasedinadose-dependentmanner.Incontrast,theStaphylococcusaureus(S.aureus)strainCowanIandother5S.aureusclinicalisolatesshowedasubstantialincreaseinbothadherenceandbiofilmformationinthepresenceofFg.TheresuitssuggestthatinS.epidermidisthefoegenemaynotbeassociatedwithbacterialadherenceandbiofilmformation.

简介：Aricepopulationconsistingof90TN1/GulyiguF3lineswasemployedtoanalyzethelinkagebetweenDNAmarkersandanewgeneWbph6(t)conferringresistancetowhitebackedplanthopper,Sogatellafurcifera.Byusingthemappingapproachofbulkedextremesandrecessiveclass,Wbph6(t)wasmappedontotheshortarmofchromosome11withageneticdistanceof21.2cMtoSSLPmarkerRM167.

简介：Objective:Toinvestigatetheexpressionofneurotrophin-3(NT-3)geneinSchwanncellsofratsciaticnerveintroducedbyanadenovirusvectorinvivo.Methods:ArecombinantadenovirusvectorforNT-3(Ad-NT-3)waspropagatedin293packagingcellsandtiteredwithtissuecultureinfectiousdose50(TCID50).Ad-NT-3wasinjecteddirectlyintotheratsciaticnerveaftertransectionandimmediaterepair.ImmunohistochemicalstainingwasemployedtodeterminetheexpressionofNT-3inSchwanncellsinratsciaticnerveandtheexpressiveintensityofthetissueslicesofthesciaticnervewasmeasuredwithLEICAM550imageanalysissystem.Results:Onthe2nddayafterinjectionofAd-NT-3,positivestainintheSchwanncellswasapparentinthevicinityofanastomosis.NT-3expressionincreasedsignificantlyonthe7thday(P<0.01)andthendecreased14-28daysafterinjection(P<0.01).TherewasnosignificantdifferenceofNT-3expressionbetweenthe14thand28thdaygroups(P>0.05).Comparedwiththe2nddaygroup,the14thand28thdaygroupsstillmaintainedarelativelyhighlevelofNT-3(P<0.01).Intactandrepairednerves,whichwereinjectedwithadenovirusencodingLacZgenes(Ad-LacZ)orphysiologicalsalineservedascontrols,showednoNT-3-positiveSchwanncells.Conclusions:AnadenovirusvectorcanbeusedtoinduceefficientlytheexpressionofNT-3geneinSchwanncellsofratperipheralnervesfollowingnerveinjuryandrepair,whichsuggeststhatneurotrophicfactorscanbeintroducedintoSchwanncellswithanadenovirusvectortopromoteperipheralnerveregeneration.

简介：雷文东，张汝刚，阎水忠，王秀琴，牟巨伟，张大为，吴Ｎｍ２３ＧＥＮＥＥＸＰＲＥＳＳＩＯＮＡＮＤＩＴＳＣＯＲＲＥＬＡＴＩＯＮＷＩＴＨＬＹＭＰＨＮＯＤＥＭＥＴＡＳＴＡＳＩＳＩＮＨＵＭＡＮＬＵＮＧＣＡＮＣＥＲ￥ＬｅｉＷｅｎｄｏｎｇ；ＺｈａｎｇＲｏｕｇｉｎｇ；...