简介：Longnon-codingRNAs(lncRNAs)refertoagroupofRNAsthatareusuallymorethan200nucleotidesandarenotinvolvedinproteingeneration.Instead,lncRNAsareinvolvedindifferentregulatoryprocesses,suchasregulationofgeneexpression.DifferentlncRNAsexistthroughoutthegenome.LncRNAsarealsoknownfortheirrolesindifferenthumandiseasessuchascancer.HOTAIRisanlncRNAthatplaysaroleasanoncogenicmoleculeindifferentcancercells,suchasbreast,gastric,colorectal,andcervicalcancercells.Therefore,HOTAIRexpressionlevelisapotentialbiomarkerfordiagnosticandtherapeuticpurposesinseveralcancers.ThisRNAtakespartinepigeneticregulationofgenesandplaysanimportantroleindifferentcellularpathwaysbyinteractingwithPolycombRepressiveComplex2(PRC2).Inthisreview,wedescribethemolecularfunctionandregulationofHOTAIRanditsroleindifferenttypesofcancers.

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简介：Objective:Toexploretheroleofthetexturefeaturesofimagesinthediagnosisofsolitarypulmonarynodules(SPNs)indifferentsizes.Materialsandmethods:Atotalof379patientswithpathologicallyconfirmedSPNswereenrolledinthisstudy.TheyweredividedintothreegroupsbasedontheSPNsizes:≤10,11-20,and>20mm.Theirtexturefeaturesweresegmentedandextracted.ThedifferencesintheimagefeaturesbetweenbenignandmalignantSPNswerecompared.TheSPNsinthesethreegroupsweredeterminedandanalyzedwiththetexturefeaturesofimages.Results:These379SPNsweresuccessfullysegmentedusingthe2DOtsuthresholdmethodandtheself-adaptivethresholdsegmentationmethod.ThetexturefeaturesoftheseSPNswereobtainedusingthemethodofgreylevelco-occurrencematrix(GLCM).Ofthese379patients,120hadbenignSPNsand259hadmalignantSPNs.Theentropy,contrast,energy,homogeneity,andcorrelationwere3.5597±0.6470,0.5384±0.2561,0.1921±0.1256,0.8281±0.0604,and0.8748±0.0740inthebenignSPNsand3.8007±0.6235,0.6088±0.2961,0.1673±0.1070,0.7980±0.0555,and0.8550±0.0869inthemalignantSPNs(allP<0.05).Thesensitivity,specificity,andaccuracyofthetexturefeaturesofimageswere83.3%,90.0%,and86.8%,respectively,forSPNssized≤10mm,andwere86.6%,88.2%,and87.1%,respectively,forSPNssized11-20mmand94.7%,91.8%,and93.9%,respectively,forSPNssized>20mm.Conclusions:Theentropyandcontrastofmalignantpulmonarynoduleshavebeendemonstratedtobehigherincomparisontothoseofbenignpulmonarynodules,whiletheenergy,homogeneitycorrelationofmalignantpulmonarynodulesarelowerthanthoseofbenignpulmonarynodules.Thetexturefeaturesofimagescanreflectthetissuefeaturesandhavehighsensitivity,specificity,andaccuracyindifferentiatingSPNs.ThesensitivityandaccuracyincreaseforlargerSPNs.更多还原

简介：Objective:Toinvestigatethepost-transcriptionalregulationofp21WAF1/CIP1byp53.Methods:TheMDA-MB-468cellshaveendogenousmutantp53andtheMCF7cellslineshavewtp53.Recombinantp53expressionandp21WAF1/CIP1inductionweredetectedbyWesternblotanalysis.Northernblotanalysiswascarriedouttoexaminewhetherchangesinp21WAF1/CIP1proteinlevelsinMCF7cellstreatedwithAdCMVp53arereflectedatthemRNAlevel.FlowcytometricanalysisofMCF7cellsfollowingoverexpressionofrecombination.Results:Theratioofp53:p21WAF1/CIP1wasbelow1attheearlystagesofAdCMVp53infection,butincreasedto1.6byday3andto9.7byday5post-infection.Asexpected,p21WAF1/CIP1expressionwasnotdetectableinMDA-MB-468cellsdespitethepresenceofhighlevelsofmutantp53protein.TheG1/SratiosinuntreatedcontrolsandAdCMVβgalinfectedMCF7cellswere1.10and1.35,respectively.ByNorthernblotanalyzingthep21WAF1/CIP1:GAPDHratiosatdifferenttimepointsagainsttheratioattimepoint0,amaximum3-foldinductionofp21WAF1/CIP1mRNAexpressionrelativetountreatedcontrolwasobservedonday1post-infection.TheflowcytometricanalysisindicatedthatMCF7cellsinfectedwithAdCMVp53undergoG1arrestatbothtimepointsstudied,withG1/Sratiosrangingfrom5.54atday1to5.65atday7.TheG1/SratiosinuntreatedcontrolsandAdCMVβgalinfectedMCF7cellswere1.10and1.35,respectively.Conclusion:Thisstudydemonstratedthatp53couldregulatep21WAF1/CIP1geneexpressionatboththetranscriptionalandpost-transcriptionallevelsinMCF7cells.Thelattermechanismmaybeinvolvedinorberesponsiblefor,theinductionofcellcyclearrestbytranscription-defectivemutantsofp53.

简介：Objective:Correctnutritionalassessmentisessentialforleukemiapatientsafterhematopoieticstemcelltransplantation(HSCT).ThisstudyaimedtoinvestigatethebestnutritionalassessmentmethodforleukemiapatientsafterHSCT,andfindthepossiblenutritionalriskofthepatientsduringthetransplantationprocessinordertointerveneinthepatientswithnutritionalrisksandundernourishedpatientstimely,sothattheentiretransplantationprocesscouldbesuccessfullycompleted.Methods:Aprospectivestudywasperformedin108leukemiapatientsafterHSCT,anddifferentnutritionalassessmentmethods,includingnutritionalriskscreening2002(NRS2002),mininutritionalassessment(MNA),subjectiveglobeassessment(SGA)andmalnutritionaluniversalscreeningtools(MUST),wereused.Theassociationsbetweennutritionalstatusofthesepatientsandnutritionalassessmentmethodswereanalyzed.Results:Atotalof108patientscompletedSGA,and99patientscompletedNRS2002,MNAandMUST.Duringthetreatmentprocess,85.2%ofthepatientslostweight,wherein,50%lostweightgreaterthan5%,and42.6%hadsignificantlyreducedfoodintake.Fornutritionalriskassessment,thepositiveratesofNRS2002,MNAandMUSTwere100%,74.7%and63.6%,respectively.Therewasasignificantdifference(P<0.05)amongthepositiveratesofNRS2002,MNAandMUST.Inundernutritionassessment,thepositiverateofSGA(83.3%)wassignificantlyhigherthanthatofMNA(17.2%)(P<0.05),andtheincidencerateofnutritionalriskamongleukemiapatients≤30yearsoldwasgreaterthanthatofpatients>30yearsold(P<0.05).Conclusions:PatientswithleukemiawereinpoornutritionalstatusduringandafterHSCT.Theleukemiapatients≤30yearsoldhadagreaterincidencerateofnutritionalrisk.Asnutritionalriskscreeningtool,thespecificityofNRS2002isnothigh,butitcanbeusedforevaluatingnutritionaldeficiencies.MNAisagoodnutritionalriskscreeningtool,butnotanadequatetoolfornutritionalassessment.Ifassessmentofundernutritionisneces

简介：目的：探讨p53家族新成员p63及p73在脑胶质瘤中的表达情况，以及在不同病理分级的表达变化。方法：采用免疫组化S-P方法检测66例不同病理分级胶质瘤p63及p73蛋白的表达情况，与置换一抗的空白对照组比较，并同时进行组间对照。结果：p63及p73在66例脑胶质瘤患者中明显表达，p63表达阳性率为42．42％，p73表达阳性率为31．82％。组间对照：p63组Ⅱ～Ⅳ级与I～Ⅱ级比较P<0．Ol：p73组Ⅲ～Ⅳ级与I～Ⅱ级比较P<0．05，Ⅱ—Ⅲ级与I～Ⅱ级比较，x^2=2.268，P>0．05。结论：p63及p73作为p53家族的新成员可能是候选的抑癌基因。

简介：Geneticchangesofseveraltumorsuppressorgenessuchasp53andpl6areinvolvedinthegenesisordevelopmentofglioma.["’]Recently,anovelgenewhichencodesaproteinwithsimilaritytop53throughoutitsDNA-binding,transactivation,andoligomerizationdomains,calledp73,wasident...

简介：客观：在乳癌学习P-glycoprotein(P-gp)的临床的意义。方法：在乳癌的60种情况中的P-gp的表示被immunohistochemistry检验。到化疗的P-gp表示和反应比较地与变形乳癌在19个病人被调查。结果：P-gp在乳癌的60个案例中的48.3%个中是积极的。P-gp表示不与病人的年龄，月经地位，包含的腋的淋巴节点的数字，临床的阶段，组织学的类型，和神经质的受体地位有关(P>0.05)。转移(62.1%)和死亡(51.7%)的频率比在否定情况中在P-gp积极案例中是更高的(16.1%对12.9%，P<0.005)。P-gp积极案例(48.3%)的5年的幸存率比否定案例(87.1%)的显著地低(P<0.05)。在收到了辅助化疗的病人，远转移比在P-gp否定案例(57.1%)(P=0.0468)中更经常发生在P-gp积极案例(94.7%)中。更多的P-gp否定病人(7/9)对化疗(P=0.0055)比积极病人(1/10)应答。结论：P-gp表示的Immunohistochemical检查在在乳癌病人预言反应到化疗和预后是有用的。P-gp确实与差的预后被联系。

简介：p16是kamb和kolnik于1994年初首先报道的一种新型抑癌基因。已证实p16基因位于人9号染色体的p21区域，其由3个外显子和2个内含子组成。这个部位的缺失、突变易发生恶性肿瘤。p16基因的重要性不亚于p53和RB肿瘤抑制基因、甚至更为重要。为了探讨p16的表达及在胃癌中的意义，

简介：Objective:TostudythedifferencesandsimilaritiesoftheantisensedrugswithdifferentstructuresonthebiologicalfunctionsofK562cells.Methods:Cytotoxiceffectsweremeasuredbyuseofacellviabilityassay.FlowcytometricanalysisandagarosegelelectrophoresisofDNAfragmentationwerealsoperformed.Theexpressionlevelofproteinwasassayedbyimmunofluorescenceusingfluoresceisothiocyanatelabel.Results:PNAtargetingthecodingregionoftheBcl-2messengerRNAcouldeffectivelyinhibitK562cellviability,down-regulatethesynthesisoftheBcl-2proteinandincreasecellapoptosis.By72haftertheBcl-2antisensePNAtreatment,K562cellsshowedmorereductioninthelevelofBcl-2proteincomparedwithcellstreatedwiththeantisenseODN.Aftertreatmentwith10μmol/LofBcl-2antisensePNAorantisenseODNfor72h,apoptoticratesofK562cellswere13.15±1.13and11.72±1.12,respectively.Furthermore,therewassignificantdifferenceinthepercentageofapoptoticcellsbetweenantisensePNAgroupandantisenseODNgroup.Conclusion:TheresultssuggestthatantisensePNAtargetingthecodingregionofBcl-2mRNAhasbetterantisenseeffectsthantheantisenseoligonucleotidesoninducingapoptosisofK562cells.

简介：调查瘤的目的淋巴的联系转移的基因；它的分子的机制。方法22690鼠标染色体cDNA微数组(包括14500知道基因；4371EST)被用来比较；分析老鼠肝细胞癌房间线Hca-F(高度淋巴的转移潜力)的基因表示侧面；Hca-P(低潜力)。结果901基因；129EST至少是起来调整的在Hca-F的2褶层房间。在表示显示出重要改变的33基因被介绍，包括endoglin(EDG)，MCAM，Cdc42ep5，F2r，D7Ertd458e，Serpinh1(HSP47)，AXL，Areg；那么上。这些基因有血管生成的函数，房间粘附，信号转导变异，房间活动性，女伴活动，蛋白质激酶活动；受体绑定。微数组与淋巴的转移模型相结合的结论cDNA可能贡献新方法；淋巴的转移研究的瘤的线索。在表示基因上的一些可能提供新奇线索给瘤的分子的机制淋巴的转移。

简介：Objective:Toexploretheeffectsofpostmastectomyradiotherapy(PMRT)onthelocoregionalfailure-freesurvival(LRFFS)andoverallsurvival(OS)ofbreastcancerpatientsunderdifferenttumorstagesandwithonetothreepositiveaxillarylymphnodes(ALNs).Methods:Weconductedaretrospectivereviewof527patientswithonetothreepositivelymphnodeswhounderwentmodifiedradicalorpartialmastectomyandaxillarydissectionfromJanuary2000toDecember2002.ThepatientsweredividedintotheT1-T2N1andT3-T4N1groups.TheeffectsofPMRTontheLRFFSandOSofthesetwopatientgroupswereanalyzedusingSPSS19.0,Pearson’sχ2-test,Kaplan-Meiermethod,andCoxproportionalhazardmodel.Results:ForT1-T2N1patients,nostatisticalsignificancewasobservedintheeffectsofPMRTonLRFFS[hazardratio(HR)=0.726;95%confidenceinterval(CI):0.233-2.265;P=0.582]andOS(HR=0.914;95%CI:0.478-1.745;P=0.784)ofthegeneralpatients.Extracapsularextension(ECE)andhighhistologicalgradeweretheriskfactorsforLRFFSandOSwithstatisticalsignificanceinmultivariateanalysis.StratificationanalysisshowedthatPMRTstatisticallyimprovedtheclinicaloutcomesinhigh-riskpatients[ECE(+),LRFFS:P=0.026,OS:P=0.007;histologicalgradeIII,LRFFS:P<0.001,OS:P=0.007]butnotinlow-riskpatients[ECE(–),LRFFS:P=0.987,OS:P=0.502;histologicalgradeI-II,LRFFS:P=0.816,OS:P=0.296].ForT3-T4N1patients,PMRTeffectivelyimprovedthelocalcontrol(HR=0.089;95%CI:0.210-0.378;P=0.001)ofthegeneralpatients,whereasnostatisticaleffectwasobservedonOS(HR=1.251;95%CI:0.597-2.622;P=0.552).Absenceofestrogenreceptorsandprogesteronereceptors(ER/PR)(–)wasanindependentriskfactor.FurtherstratificationanalysisindicatedastatisticaldifferenceinLRFFSandOSbetweenthehigh-riskpatientswithER/PR(–)receivingPMRTandnotreceivingPMRT[ER/PR(–),LRFFS:P=0.046,OS:P=0.039].However,PMRThadabeneficialeffectonthereductionoflocoregionalr

简介：目的：检测信号分子JAK2／STAT3在食管鳞癌组织样本中的表达，并探讨成纤维细胞生长因子诱导14（Fn14）表达与JAK2／STAT3信号通路的相关性。方法采用免疫组织化学法检测118例食管癌组织及配对正常食管黏膜组织中Fn14、p-JAK2和p-STAT3蛋白的表达情况。分析Fn14与p-JAK2、p-STAT3蛋白表达的相关性及三者表达与食管鳞癌临床病理特征的关系。结果Fn14阳性表达主要定位于细胞膜和胞浆，p-JAK2、p-STAT3阳性表达主要定位于细胞质和胞核。Fn14、p-JAK2、p-STAT3在食管鳞癌中的阳性表达率分别为51．7％（61／118）、50．9％（60／118）、57．6％（68／118），均高于正常食管黏膜鳞状上皮组织的4．2％（5／118），差异有统计学意义（P＜0．05）。Fn14、p-JAK2及p-STAT3蛋白表达与分化程度、TNM分期有关（P＜0．05），而与性别、年龄均无关（P＞0．05）。Fn14与p-JAK2、p-STAT3在食管鳞癌不同分化程度组织中及不同TNM分期中均呈正相关（P＜0．05）。结论Fn14、p-JAK2、p-STAT3在食管鳞癌中高表达，可能与食管鳞癌的发生、发展有关。

简介：目的P16在宫颈癌中表达升高且很少发生突变。本文的研究旨在探讨P16在宫颈癌中的高表达是否受PRb、Bcl-2、P73表达的影响。方法对61例宫颈癌进行P16、PRb、Bcl-2、P73的免疫组化染色，观察P16表达与PRb的阳性率及作为PRb结合E2f功能指示器的Bcl-2和P73的阳性率的关系。结果P16高表达者在PRb、Bcl-2或P73阳性表达病例中分别占63．7％、61．0％、68．0％，而在PRb、Bcl-2或P73阴性病例中分别占43．6％、30．8％、38．9％；P16低表达者在PRb、Bcl-2或P73阳性表达病例中分别占36．3％，39．0％、32．0％，而在PRb、Bcl-2或P73阴性病例中分别占56.4％，70．2％、61．1％。经，检验分析，P16过表达与PRb含量关系不密切，但与Bcl-2和P73阳性率趋势一致。结论宫颈癌中P16表达升高不受PRb阳性率影响，而可能是由于PRb结合E2f功能减低所致。

简介：Objective:TheUnionforInternationalCancerControl(UICC)Node(N)classificationisthemostcommonusedstagingmethodfortheprognosisofgastriccancer.Itdemandsadequate,atleast16lymphnodes(LNs)tobedissected;thereforedifferentstagingsystemswereinvented.Methods:BetweenMarch2005andMarch2010,164patientswereevaluatedattheDepartmentofGeneralSurgeryintheKenézyGyulaHospitalandattheDepartmentofGeneral,ThoracicandVascularSurgeryintheKaposiMórHospital.The6th,7thand8thUICCN-stagingsystems,thenumberofexaminedLNs,thenumberofharvestednegativeLNs,themetastaticlymphnoderatio(MLR)andthelogoddsofpositiveLNs(LODDS)weredeterminedtomeasuretheir5-yearsurvivalratesandtocomparethemtoeachother.Results:Theoverall5-yearsurvivalrateforallpatientswas55.5%withamedianoverallsurvivaltimeof102months.Thetumorstage,gender,UICCN-stages,MLRandtheLODDSweresignificantprognosticfactorsforthe5-yearsurvivalwithunivariateanalysis.The6thUICCN-stagedidnotfollowtheadequateriskincomparingN2vs.N0andN3vs.N0withmultivariateinvestigation.ComparisonofperformancesoftheresidualNclassificationsprovedthattheLODDSsystemwasfirstinthepredictionofprognosisduringtheevaluationofallpatientsandincaseswithlessthan16harvestedLNs.TheMLRgavethebestprognosticpredictionwhenadequate(morethanorequalto16)lymphadenectomywasperformed.Conclusions:WesuggesttheapplicationofLODDSsystemroutinelyinwesternpatientsandtheusageofMLRclassificationincaseswithextendedlymphadenectomy.

简介：Objective:Toidentifydifferentiallyexpressedlongnon-codingRNAs(lncRNAs)involvedinthemetastasisofepithelialovariancancer.Methods:AninvitroinvasionassaywasperformedtovalidatetheinvasivecapabilityofSKOV3andSKOV3.ip1celllines.TotalRNAwasthenextracted,andmicroarrayanalysiswasperformed.Moreover,ninelncRNAswereselectedforvalidationusingRT-qPCR.Results:ComparedwiththeSKOV3cells,theSKOV3.ip1cellssignificantlyimprovedintheinvitroinvasiveactivity.Ofthe4,956lncRNAsdetectedinthemicroarray,583and578lncRNAswereupregulatedanddownregulated,respectively,inSKOV3.ip1cells,comparedwiththeparentalSKOV3cells.SevenoftheanalyzedlncRNAs(MALAT1,H19,UCA1,CCAT1,LOC645249,LOC100128881,andLOC100292680)confirmedthederegulationfoundbymicroarrayanalysis.Conclusion:LncRNAsclustersweredifferentiallyexpressedinovariancancercellswithvaryingmetastaticpotentials.ThisresultindicatesthatsomelncRNAsmightexertapartialorkeyroleinepithelialovariancancermetastasis.FurtherstudiesshouldbeconductedtodeterminetherolesoftheselncRNAsinovariancancermetastasis.

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简介：先于尖锐myeloid白血病(AML)的Myeloid肉瘤(MS)是稀罕的，作为尖锐针的绳索压缩介绍是它甚至稀罕。这里，我们报导myeloid肉瘤病人，其结果不同的二个新案例。有针的MS前面的AML的27个病人迄今为止被报导了，包括在这篇文章介绍的二个案例。外科的解压缩在27个病人中的25个被执行，并且这些中的23个收到了另外的anti-AML治疗。就我们在文学的病人和出版案例而言，我们建议immunohistochemical学习在到达MS，和那个紧急情况手术的正确诊断到针的MS是的将切除起一个必要作用做神经功能恢复的可得到的治疗，并且疾病必须与类似于过去常在肿瘤的切除术或照耀以后尽快对待AML那的集中的化疗被治疗。

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简介：目的:探讨p16基因外显子2的缺失和突变与胃癌发生发展的关系.方法:应用新鲜组织标本基因组DNA抽提、PCR-SSCP分析的方法,对30例胃癌及癌旁组织中p16基因外显子2的缺失和突变进行检测.结果:胃癌组织样本中p16基因外显子2的缺失率为10.00%,突变率为10.00%,两者之和为20.00%,癌旁组织样本中未发现缺失和突变,统计学分析有显著性差异(P<0.01).结论:p16基因外显子2的缺失和突变与胃癌的发生具有一定的相关性.