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简介：Theintensitydistributionsofahigh-powerbroadbandlaserbeampassingthroughanonlinearopticalmediumwithdefectsandthenpropagatinginfreespaceareinvestigatedbasedonthegeneralnonlinearSchr¨odingerequationandthesplit-stepFouriernumericalmethod.Theinfluencesofthebandwidthofthelaserbeam,thethicknessofthemedium,andthedefectsonthelightintensitydistributionarerevealed.Wefindthatthenonlinearopticaleffectcanbesuppressedandthattheuniformityofthebeamcanbeimprovedforahigh-powerbroadbandlaserbeamwithappropriatewidebandwidth.Itisalsofoundthat,underthesameincidentlightintensity,athickermediumwillleadtoastrongerself-focusingintensity,andthattheinfluenceofdefectsintheopticalelementsontheintensityisstrongerforanarrowbandbeamthanforabroadbandbeam.

简介：目的探讨MesenPRORSMedium对人脂肪干细胞增殖与分化的影响。方法应用MesenPRORSMedium与DMEM＋10％FBS两种培养基培养人脂肪干细胞，比较两者在细胞形态、体外增殖能力、成纤维细胞集落形成（CFU—F）能力及多向分化潜能的差异。结果MesenPRORSMedium培养的人脂肪干细胞具有良好的细胞形态，在增殖速度、细胞集落，以及成脂、成骨及成软骨能力等方面均优于DMEM＋10％FBS。结论MesenPRORSMedium是人脂肪干细胞优良的培养基。

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简介：WiththeeconomicdevelopmentandtheaccelerationofmotorizationinChina,thenumberofprivatecarsincreasesrapidlyinurbanareas.However,thelimitofurbanresourcesandthecontradictionbetweentrafficsupplyanddemandareincreasinglyprominentinlargecities,whileprivatecarconsumptionhasbeenanew'light'inmediumandsmallcities.Consumers’behaviorsdifferfromregiontoregion.Aimingattheaboveproblems,weformulatestructuralequationmodelingandcarryoutempiricalresearch.Inthispaper,acomparativestudyaboutthemaininfluentialfactorsthataffecttheconsumptionofprivatecarsinlargeandsmallcitiesinChinaismade,anditishopedthatsomeguidanceforpolicyrecommendationscanbeobtained.

简介：Ⅰ.AnalysisofFactorsAffectingIntellectualPropertyPledgeFinancingSincetechnologyisamajordrivingforceforeconomicrevitalization,eachcountryattachesgreatimportancetotheadjustmentofindustrialstructure,highlightsthedevelopmentofhigh-technologyenterprises,andpursuestheroadofinnovationanddevelopment.

简介：BackgroundPrematureventricularcontractions(PVCs)arefrequentlyseeninchildren.However,therearelesssystematicandlongerfollowing-upstudiesexaminingtheprognosisofPVCsinchildren.TheaimofthisstudywastoevaluatethemediumtolongtermprognosisofPVCsinchildhoodandwhetherthereisadifferentialprognosisindifferentprimarydiseasesofPVCs.MethodsThisstudyreviewedthedataof106pediatricpatients(49F/57M,7.5±3.8years)seenattheAffiliatedHospitalofQingdaoUniversitywiththediagnosisofPVCsbetween1999and2005.Dataondemographics,clinicalpresentation,laboratorytests,andechocardiogramsofpatientswereextractedfromtheavailableclinicalrecords.ResultsAtotalof35(33.0%)childrenpresentedwithPVCsduetomyocarditis,7(6.6%)duetocardiomyopathies,7(6.6%)duetomitralvalveprolapse(MVP),10(9.4%)duetooperationforcongenitalheartdisease(O-CHD),16(15.2%)duetoleftventricularfalsetendons(LVFT),and31(29.2%)duetounknowncause.HolterdidnotshowPVCsduringfollow-upperiodin100%ofmyocarditispatients,57%ofcardiomyopathypatients,71%ofMVPpatients,60%ofO-CHDpatients,88%ofLVFTpatients,87%ofunknowncausepatients.ThePVCsdisappearedin93%ofpatientswhodidnotuseanti-arrhythmicdrugsandin76%ofpatientswhousedantiarrhythmicdrugs.Therewasnoasignificantdifferenceinprognosisbetweenmyocardialnutritioncombinedwithintravenousinjectionofimmunoglobulin(IVIG)groupandpropafenonegroup.ConclusionsPVCscausedbydifferentprimarydiseaseshasafavorableprognosisinchildren.Usually,thePVCswillreduceevendisappearduringfollow-up.ThepatientswithPVCsduetomyocarditisshouldbepreferredusemyocardialnutrientcombinedwithIVIG.

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简介：TheDonnelltheoryofshellisappliedtodescribeshellmotionandlayermotionisdescribedbymeansofthree-dimensionalNavierequations.Usingdeformationharmoniousconditionsoftheinterface,theeffectsofstiffenersandlayeraretreatedasreverseforcesandmomentsactingonthecylindricalshell.Instudyingtheacousticfieldproducedbyvibrationofthesubmergedring-stiffenedcylindricalcoatedshell,thestructuredynamicequation,Helmholtzequationinthefluidfieldandthecontinuousconditionsofthefluid-structureinterfacecomposethecou-plingvibrationequationofthesound-fluid-structure.Theextractofsoundpressurecomesdowntotheextractofcouplingvibrationequation.Byuseofthesolutionoftheequation,theinfluencesofhydrostaticpressure,physicalcharactersandgeometricparametersofthelayeronsoundradiationarediscussed.

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简介：Wedemonstratetheefficientgenerationofcoherentlightinafour-leveldouble-cascadeatomicmediumbycontinuous-wavelow-intensitylaserradiation.Wederivethecorrespondingexplicitanalyticalexpressionsforthegeneratedfour-wavemixing(FWM)field.DependenciesoftheintensityofthegenerateFWMfieldonthepropagationdistance,ontheinput-waveintensity,andonthephotondetuningareinvestigated.Toconclude,wealsogiveabriefdiscussionontheexperimentalrealizationoftheproposedscheme.

简介：WehavestudiedthefragmentationprocessinheavyioncollisionssystematicaIlybyusingIsospin-dependentQuantumMolecularDynamics(IQMD)modelandfoundthatthemultiplicityofintermediatemassfragmentsN_(imf)canbeusedasaprobetoextracttheinformationofin-mediumnmuleon-nucleoncrosssection.Fig.1shows

简介：Anumericalstudyofanon-Darcymixedconvectiveheatandmasstransferflowoveraverticalsurfaceembeddedinaporousmediumundertheefectsofdoubledispersion,melting,andthermalradiationisinvestigated.Thesetofgoverningboundarylayerequationsandtheboundaryconditionsistransformedintoasetofcouplednonlinearordinarydiferentialequationswiththerelevantboundaryconditions.ThetransformedequationsaresolvednumericallybyusingtheChebyshevpseudospectralmethod.Comparisonsofthepresentresultswiththeexistingresultsintheliteraturearemade,andgoodagreementisfound.Numericalresultsforthevelocity,temperature,concentrationprofiles,andlocalNusseltandSherwoodnumbersarediscussedforvariousvaluesofphysicalparameters.

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简介：TheeffectofTPA,apotenttumorpromoter,onSSV-NIH3T3cellsinserum-freemediumwasinvestigated.TPAstimulatedDNAsynthesisofSSV-NIH3T3cellsonthethirddayofcultureinSFM.InSDS-PAGFofmediumconditionedbyTPA-treatedSSV-NIH3T3cells(inSFM+TPA),theamountsoffourproteinsof31.0Kd,28.5Kd,25.5Kdand13.5Kdstrikinglyincreasedoverthatofnon-TPA-treatedcounterpart(inSFM).ThePDGF-likeactivitywasalsodetectedinCMofSFM+TPA.WheninsulinandEGFweredrownofftheSFM+TPA(SFM-Ins-EGF+TPA),TPAlostitsabilitytostimulateDNAsynthesisofSSV-NIH3T3cellsonthethirddayandSDS-PAGEoftheconditionedmediumshowedthattheamountsofthefourproteinsnotedabovegratelyreduced.However,cellsinSFM-Ins-EGF+TPAwereinalmostthesamegrowthconditionascellsincompleteSFM+TPAonthethirddayofculture.Resultswerediscussedinthepaper.

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简介：AprotocolformicropropagationusingnodalexplantsfrommaturePinusmassonianatreeshasbeendeveloped.Timeofexplantcollectioniscrucialfortheinitialsuccessofasepticculture.ExplantscollectedinearlyMarchgavethehighestpercentageofexplantsurvival(64.5%)andshoot-formingpercentage(52.3%).Thidiazuron(TDZ)concentrationsignificantlyinfluencedshootformation;4lMTDZwasoptimum,with4.8shootsproducedperexplantwithameanlengthof7.1cmafter120daysofculture.Regeneratedshootsrootedfor60daysinbasicmediumwith1lMNAAwerereadyforgrowthinpots.ThisisthefirstreportonplantletregenerationinvitrofrommaturetreesofP.massonianathatprovidesareliablemethodforpropagatingselectedelites.

简介：Inthisletterthethree-dimensionalnonlinearHelmholtzequationisinvestigated,whichdescribeselectro-magneticwavepropagationinanonlinearKerr-typemediumsuchthatsixteenfamiliesofnewJacobiellipticfunctionsolutionsareobtained,byusingourextendedJacobianellipticfunctionexpansionmethod.Whenthemodulusm→1or0,thecorrespondingsolitarywavesincludingbrightsolitons,darksolitonsandnewlinesolitonsandsinglyperiodicsolutionscanbealsofound.

简介：AbstractThere is increasing evidence that cell-free DNA (cfDNA) in spent culture media (SCM) can be amplified for genetic testing. Therefore, this paper reviews the characteristics of cfDNA, including its fragment size, amount, origin, as well as some factors affecting the success rate of its amplification, together to provide researchers with a more comprehensive perspective on embryonic cfDNA. The origin of cfDNA in SCM is complicated and poses challenges to the interpretation of genetic test results. Advanced molecular techniques should distinguish between embryonic and contaminated DNA to maximize the success rate of amplification and analysis. Recent data showed that the type of culture medium, assisted hatching or not, the type of amplification kit, and fresh or thawed embryos were not related to the success rate of amplification, but the length of culture time might affect the success rate. The longer culture time, the more cfDNA is available in the SCM. Then we focused on the concordance between trophectoderm (TE), inner cell mass, whole embryo, and embryonic cfDNA. Despite successful amplification, the concordance between TE and embryonic cfDNA was low. In summary, non-invasive genetic testing using SCM could represent a major advance in future single embryo selection, however, contamination and timing for media collection are key factors affecting the results, and current non-invasive cfDNA testing should not be directly applied to clinical practice. Further research is needed to improve the methods used for testing techniques and genetic analysis to achieve greater accuracy and trace its origins before it can be used in the clinics.