简介：GFAPisaspecificantigenofglialelement,butAlpha-1-antichymotrypsinhasnotbeenreportedintheliterature.Alpha-1-antichymotrypsinwasguidedbyGFAPusingPAPmethodtotheastrocytesof137gliomas.120(87%)gliomaswerepositiveforAlpha-1-antichymotrypsin.Ofthese120gliomas,86(72%)gavediffusedistribution,17(14%)gavefocaldistribution,and17(14%)gavescattereddistributions.Alpha-1-antichymotrypsiningliomatissuemaybeanimportanttumormarkerfordiagnosis.

简介：ThehistogensisofextramammaryPaget’sdiseasehasnotbeensolvedandremainedcontroversial.EightcasesofextramammaryPaget’sdiseaseofgeni-tocruralregionwereinvestigatedbyalcianblueandPASstainandimmunoreactionofanti-CEAandanti-keratin.Itwasfoundthatthepatternandintensityofalcianblue,PASstainingwereidenticalforPagetcellsandsecretorycellsofapocrinesweatgland;andCEAimmunoreactivitywasuniformlyobservedinbothPagetcellsandeccrinesweatgland.Thekeratinimmunoreactionwaspositiveinkeratinocytes,apocrineandeccrinesweatgland,whereasPagetcellswerenegative.TheseresultssuggestedthatPagetcellsofextramammaryPaget’sdiseasecouldbederivedfrommultipotentialepidermalgermcells.

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简介：Extrapulmonarysmallcellcarcinoma(EPSCC)isarareneoplasmcomprising2.5%to5%ofsmallcellcarcinomas(SCCs).BladderSCCisthemostcommonsiteofgenitourinarytract.PrimaryrenalSCCisextremelyrare.WereportacaseofprimarySCCofthekidneywhichisrarelyreportedintheurinarytractandpresentsanaggressiveclinicalpicture.A59-year-oldfemalevisitedaurologicclinicwithcomplaintofpersistentleftflanksoreness10yearsafterundergoingrenaltransplantation.Abdominalcomputedtomographyshowedaleftrenalpelvistumor.Afterthepatientreceivedleftnephroureterectomywithbladdercuffresection,herpathologyresultsshowedSCC.Aftersurgery,shereceivedadjuvantsystemicchemotherapy,andherrecoveryhasbeenuneventfulasof8months.PrimaryrenalSCCpresentswithanadvancedtumorstageandashortmediansurvivalperiod,thereforeearlyinterventionandclosefollow-uparerecommended.

简介：Renalcellcarcinoma(RCC)accountsforapproximately3%ofallcancercases.RCCsusuallymetastasizetothelungs,bones,liver,orbrain.Only<1%ofpatientswithbonemetastasesmanifestedclavicularRCCmetastases.Thus,clavicularmetastasisastheinitialpresentationofRCCisextremelyrare.WereportapatientwithRCCmetastasistotheleftclavicle,whichwasfirstpresentedwithpaincausedbyapathologicalfracture.Magneticresonanceimagerevealedarenaltumor,andtechnetium-99m–methylenediphosphonatebonescintigraphyshowedmultipleosseousmetastases.Thepatienteventuallyunderwentsurgerytoremovethelateralendoftheleftclavicleandrightkidney.Histopathologyrevealedrenaltumorandclearcellcarcinomaintheclavicle.Finally,wereview17casesofclavicularmetastasesoriginatingfromdifferentmalignancies.

简介：A60-year-oldwomanwithsquamouscellcarcinomaintherightlungwassuccessfullytreatedwithfourcyclesofcombinationchemotherapyaftersurgery,andcompleteremissionwasachieved.However,thepatientdevelopedmyelodysplasticsyndrome(MDS)RAEB-2withmyelofibrosisafterremission,possiblybecauseofchemotherapyorDNAmethylation.Thepatientrespondedwelltodacitabine(Dacogen),suggestingthatDNAhypomethylationagentscanbeapromisingtherapytoretardtheprogressionofasecondtumororcarcinoma.

简介：ＳＴＵＤＹＯＮＭＥＴＡＳＴＡＳＩＳＡＳＳＯＣＩＡＴＥＤＧＥＮＥＳＣＲＥＥＮＥＤＢＹＭＯＮＯＣＬＯＮＡＬＡＮＴＩＢＯＤＹＨＩＬＱｉＴｅｎｇｐｉｎｇ齐藤平；Ｚｈａｎｇｐｅｉｊｉ张沛基；ＷｅｉＳｈｕｇｕａｎｇ魏曙光；ＣｈｅｎＤｏｎｇ陈东；ＬｉＲｅｎ李仁；Ｗ...

简介：TheallelicdistributionofEcoRIandBamHIfragmentsofrasfamilygenesbetweenthehumanprimarygastriccancertissuesandthecorrespondingadjacentnormaltissuesdidnotshowanydifferences.ThreegenotypesofBamHIrestrictionfragmentslengthpolymorphismofc-H-raswererevealed.NosignificantdifferencesintheRFLPswereobservedbetweennormalindividualsandgastriccancerpatients.Fourprotooncogenes,c-H-ras,N-ras,c-mycandc-fos,werefoundtobetranscriptionallyactiveinthegastriccancertissuesinsomecasesexamined.Thecomparisonoftheexpressionoftheseoncogenesbetweenthemalignanttissuesandthecorrespondingnormaltissuesshoweddifferentialpatterns.Theexpressionofc-H-rasatcellularlevelwasdetectedwithinsituhybridization.Theenhancedexpressionofc-H-rasinthegastriccancercellswasdemonstrated,butthedegreeoftheexpessionamongthecancercellswasshowntobeheterogeneous.Inaddition,theenhancedexpressionofc-H-raswasseeninthei

简介：Objective:Toexploretheclinicopathologicalfeatures,surgicaltreatmenttechniques,andprognosticriskfactorsofintrahepaticcholangiocarcinoma(ICC).Methods:Atotalof104ICCcaseswerecollectedfromJanuary2008toDecember2013atTianjinMedicalUniversityCancerInstituteandHospitalanddividedintothehepatichilumlymphadenectomy(HLL,21cases),extendedhepatichilumlymphadenectomy(EHLL,12cases),andnon-lymphadenectomy(NL,71cases)groups.Theclinicaldataofthepatientswereretrospectivelyanalyzed,andtheprognosticdifferenceswerecomparedamongdifferentgroups.Results:The1-,2-,and3-yearoverallsurvival(OS)ratesofallcaseswere72.1%,56.1%,and43.7%,respectively.Themediansurvivaldurationwas34months.The1-,2-,and3-yearOSratesoftheHLLgroup(42.9%,28.6%,and28.6%,respectively)weresignificantlylowerthanthoseoftheNLgroup(78.9%,62.5%,and47.8%,respectively).Meanwhile,the1-,2-,and3-yearOSratesoftheEHLLgroup(75.0%,56.1%,and33.3%,respectively)werenotsignificantlydifferentfromthoseoftheothertwogroups.Univariateanalysisshowedthatage,gender,AmericanJointCommitteeonCancer(AJCC)stage,differentiation,ferritin(Fer),carbohydrateantigen19-9(CA19-9)andcarcinoembryonicantigen(CEA)levels,lymphnodemetastasis(LNM),andlymphnodedissection(LND)wereprognosticfactorsforthelong-termsurvivalofICC.Meanwhile,multivariateanalysisrevealedthatage,AJCCstage,differentiation,Ferlevels,andLNMwereindependentriskfactorsforsurvival.Conclusions:ICCpatientswillnotbenefitfromlymphadenectomyintheabsenceofLNM.However,systematiclymphadenectomymayimproveICCoutcomesifthelocationoflymphaticmetastasisisknown.Age,AJCCstage,differentiation,Ferlevel,andLNMareindependentriskfactorsforsurvivalinICC.

简介：客观：为了学习表达式和apoptosis的临床的价值，在乳癌控制基因bcl-2和bax。方法：在在1996在我们的医院里从操作获得的41乳癌的蛋白质bax和bcl-2与正常的胸纸巾用ABCimmunohistochemical污点试金并且与10个盒子相比被检测。结果：在正常的胸组织的bax的积极的率是90%并且在乳癌是59%，与他们之间的重要统计差别(P<0.05)，但是在bcl-2没有统计差别蛋白质表示。在41乳癌之中，有淋巴节点转移(21个盒子)的组有显然低的bax表示(43%)和高bcl-2表示(76%)，给没有淋巴节点转移的组显示出重要差别(P<0.05)。结论：bcl-2的antiapoptosis功能是比在乳癌的bax强壮的。蛋白质bax和bcl-2试金可能在理解乳癌的生物行为是有用的。

简介：Pheochromocytomaisatumorarisingfromneuroectodermalchromaffintissuesintheadrenalglandorextra-adrenalparaganglia(paragangliomas).Theprevalenceofthetumoris0.1%-0.6%inthehypertensivepopulation,ofwhich10%-20%aremalignant.Pheochromocytomaproduces,stores,andsecretescatecholamines,aswellasleadstohypertensivecrisis,arrhythmia,angina,andacutemyocardialinfarctionwithoutcoronaryarterydiseases.Wereportacaseofacutecoronarysyndrome(ACS)withafinaldiagnosisofmultipleendocrineneoplasiawithpheochromocytomaandmedullarythyroidcarcinoma(MTC).

简介：DearFriendsandColleaguesOnbehalfoftheorganiingCommittee,wearepleasedtowelcomeyoutoparticipateinthe2~(nd)InternationalSymposiumonTranslationalCancerResearchtobeheldonAugust18~(th)-20(th),2016inTianjin,China.In2002,TianjinMedicalUniversityCancerInstituteandHospital,togetherwithTheUniversityof

简介：Acombinedhistopathological,mucinhistochemi-calandimmunohistochemicalstudyofthetransitionalmucosa(TM)adjacenttocolorectalcancerispresented.Twenty-sixresectedspecimenswerestudiedbyhematoxylinandeosin(HE)andhighirondiamine-alcianblue(HID-AB).Carcinoem-bryonicantigen(CEA)wasdemonstratedbyperoxi-daseantiperoxidass(PAP)technique.TheappearanceoftheTMisusuallythicker,longeranddilatedcryptswithincreasedimmatureandintermediatecells.VariableamountofsialomucinsanddecreasesulphomucinscontentaswellasincreasedCEAcontentarefoundintheTM.Thesechangesarenotseeninnon-transitionalzoneandnormalcolorectalmucosa.ItissuggestedthatthemucinchangesandexpressionofCEAintheTMmayindicateanearlyprimarypremalignantchangesandmaybeoneofthereasonsfortheTMaffectingtheprognosisofpatientswithlargebowelcancerafterradicalresection.

简介：Objective:TostudytheeffectofantisenseVEGFRNAonratC6gliomasinvivoandfindoutthefeasibilityofantiangiogenesistherapywithantisenseVEGFRNAformalignantgliomas.Methods:ParentalratC6gliomacellsandC6cellstransfectedwithantisenseVEGFcDNAwereimplantedintracerebrallyandsubcutaneouslyintoSDratsascontrolandtransfectedgroup.RatsbearingcerebralandsubcutaneousC6gliomasweretreatedwithantisenseVEGFcDNAastreatedgroupandsenseVEGFcDNAandemptyvectorascontroloftreatedgroup.Thegeneralmanifestation,survivaltime,MRIandhistopathologicalchangesofallratswereobserved.Thevolumeofsubcutaneouslyimplantedtumorswasdeterminedregularly.InsituhybridizationandimmunohistochemicalstainingwereusedfordetectionofVEGFgeneexpressionofgliomaswhilePCNAimmunostainingandTUNELmethodforexaminationofproliferationactivityandapoptosisofgliomas,respectively.Results:Thesurvivaloftheratsintransfectedandtreatedgroupwasprolonged.Thereweretworatssurvivingover90dinthetreatedgroupandtheirtumorsdisappeared.TheVEGFgeneexpression,thenumberofmicrovesselsandtheproliferationactivityweredecreasedandalargeamountofapoptoticcellscouldbefoundincerebralandsubcutaneousgliomasintreatedandtransfectedgroups.Conclusion:VEGFisoneofthecandidategenesforgenetherapyofmalignantgliomas.AntisenseVEGFRNAcombinedwithothertherapiesshouldbestudiedfurtherforenhancingthetherapeuticeffectofmalignantgliomas.

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简介：Toobservetheprocessofinvasion,retinaofratwasusedasamodeltosubstitutetheinnerlimitingmembraneofretinaforthebasementmembrane.RetinainvadedbyesophagealcarcinomacellsandB16melanomacellsupontheinnerlimitingmembranewasstudiedbyscanningandtransmissionelectronmicroscopy.Theresultsshowedthattheinnerlimitingmembranewasdestroyedbybothkindsoftumorcells.Theprocessofdestructionwasfollowedbyaseriesoftransformationsintheinnerlimitingmembrane,i.e.folding,swelling,thickening,andgranularchange.Theinnerlimitingmembranewasdissolvedfocallyasaresultoftransformation,andthentumorcellsinvadedtheretinathroughthesedissolvedregions.Itseemsthat,asabarrier,theinnerlimitingmembraneplaysasimilarroleasthebasementmembrane.

简介：AmethodisdescribedwhichpermitstransmissionelectronmicroscopeofsinglecellstreatedwithHpDpluslasermicroirradiation.Thepreselectedsinglecellthatwasirradiatedbylaserunderlightmicroscopeandfollowedfixation,embeddedandsectioningisexaminedunderelectronmicroscope.Theresultsdemonstratedthatatthelightdoseof1.88ml/μm2notonlytheirradiatednucleolusappearedtransparentregion,buttheotherpartssuchasnon-irradiatedmitochondriaincytoplasmcanalsobedamaged.Whenpartialcytoplasmisirradiatedwiththelightdoseof4.50ml/μm2,thedamagesappearinallcytoplasm,butthereislittlechangeinthenucleus.Theexperimentalresultsalsodemonstratethatcytoplasmismoresensitivethannucleus.ItisthemitochondriaincytoplasmthatareverysensitivetoHpDpluslaser.

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